{"id":"https://openalex.org/W4413385803","doi":"https://doi.org/10.1093/nar/gkag451","title":"An in vivo parallelized reporter assay to uncover tissue-specific splicing regulatory sequences in a multicellular animal","display_name":"An in vivo parallelized reporter assay to uncover tissue-specific splicing regulatory sequences in a multicellular animal","publication_year":2026,"publication_date":"2026-05-02","ids":{"openalex":"https://openalex.org/W4413385803","doi":"https://doi.org/10.1093/nar/gkag451","pmid":"https://pubmed.ncbi.nlm.nih.gov/42179040","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/13199693"},"language":"en","primary_location":{"id":"doi:10.1093/nar/gkag451","doi":"https://doi.org/10.1093/nar/gkag451","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkag451","pdf_url":null,"source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1093/nar/gkag451","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5066484147","display_name":"Sanjana Bhatnagar","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Sanjana Bhatnagar","raw_affiliation_strings":["Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Jade Ho","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1330342723","display_name":"Mayo Clinic","ror":"https://ror.org/02qp3tb03","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1330342723"]},{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA","US"],"is_corresponding":false,"raw_author_name":"Jade Ho","raw_affiliation_strings":["Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5","Mayo Clinic Alix School of Medicine, Mayo Clinic College of Medicine and Science 00 1st SW, Rochester MN, 55905"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Mayo Clinic Alix School of Medicine, Mayo Clinic College of Medicine and Science 00 1st SW, Rochester MN, 55905","institution_ids":["https://openalex.org/I1330342723"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Nour H Sadek","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]},{"id":"https://openalex.org/I2801317318","display_name":"Hospital for Sick Children","ror":"https://ror.org/057q4rt57","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801317318"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Nour H Sadek","raw_affiliation_strings":["Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5","Genetics and Genome Biology Program, The Hospital for Sick Children, 170 Elizabeth St, Toronto, ON M5G 1E8"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Genetics and Genome Biology Program, The Hospital for Sick Children, 170 Elizabeth St, Toronto, ON M5G 1E8","institution_ids":["https://openalex.org/I2801317318"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Isha Singh","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Isha Singh","raw_affiliation_strings":["Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111398411","display_name":"Michael Zoberman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Michael Zoberman","raw_affiliation_strings":["Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065958637","display_name":"Bina Koterniak","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Bina Koterniak","raw_affiliation_strings":["Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100761345","display_name":"Yufang Liu","orcid":"https://orcid.org/0000-0003-4698-8301","observed_orcids":["https://orcid.org/0000-0003-4698-8301"]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Yufang Liu","raw_affiliation_strings":["Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005571486","display_name":"Daniel D. Fusca","orcid":"https://orcid.org/0009-0007-1679-2398","observed_orcids":["https://orcid.org/0009-0007-1679-2398"]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Daniel D Fusca","raw_affiliation_strings":["Department of Ecology and Evolutionary Biology , 25 Willcocks Street, Toronto, Ontario, M5S 3B2 ,","Department of Ecology and Evolutionary Biology, 25 Willcocks Street, Toronto, Ontario, Canada, M5S 3B2"],"raw_orcid":"https://orcid.org/0009-0007-1679-2398","affiliations":[{"raw_affiliation_string":"Department of Ecology and Evolutionary Biology , 25 Willcocks Street, Toronto, Ontario, M5S 3B2 ,","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Department of Ecology and Evolutionary Biology, 25 Willcocks Street, Toronto, Ontario, Canada, M5S 3B2","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011015519","display_name":"Asher D. Cutter","orcid":"https://orcid.org/0000-0001-7141-0013","observed_orcids":["https://orcid.org/0000-0001-7141-0013"]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Asher D Cutter","raw_affiliation_strings":["Department of Ecology and Evolutionary Biology , 25 Willcocks Street, Toronto, Ontario, M5S 3B2 ,","Department of Ecology and Evolutionary Biology, 25 Willcocks Street, Toronto, Ontario, Canada, M5S 3B2"],"raw_orcid":"https://orcid.org/0000-0001-7141-0013","affiliations":[{"raw_affiliation_string":"Department of Ecology and Evolutionary Biology , 25 Willcocks Street, Toronto, Ontario, M5S 3B2 ,","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Department of Ecology and Evolutionary Biology, 25 Willcocks Street, Toronto, Ontario, Canada, M5S 3B2","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086545231","display_name":"Alan M Moses","orcid":"https://orcid.org/0000-0003-3118-3121","observed_orcids":["https://orcid.org/0000-0003-3118-3121","https://orcid.org/0000-0001-7099-8257"]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Alan M Moses","raw_affiliation_strings":["Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5"],"raw_orcid":"https://orcid.org/0000-0003-3118-3121","affiliations":[{"raw_affiliation_string":"Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5","institution_ids":["https://openalex.org/I185261750"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5063166108","display_name":"John A. Calarco","orcid":"https://orcid.org/0000-0002-2197-7801","observed_orcids":["https://orcid.org/0000-0002-2197-7801"]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]}],"countries":["CA"],"is_corresponding":true,"raw_author_name":"John\u00a0A Calarco","raw_affiliation_strings":["Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5"],"raw_orcid":"https://orcid.org/0000-0002-2197-7801","affiliations":[{"raw_affiliation_string":"Department of Cell and Systems Biology , 25 Harbord Street, Toronto, Ontario, M5S 3G5 ,","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Department of Cell and Systems Biology, 25 Harbord Street, Toronto, Ontario, Canada, M5S 3G5","institution_ids":["https://openalex.org/I185261750"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5063166108"],"corresponding_institution_ids":["https://openalex.org/I185261750"],"apc_list":{"value":3992,"currency":"USD","value_usd":3992},"apc_paid":{"value":3992,"currency":"USD","value_usd":3992},"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.00466249,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"54","issue":"10","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alternative-splicing","display_name":"alternative splicing","score":0.925000011920929},{"id":"https://openalex.org/keywords/rna-binding-proteins","display_name":"RNA-binding proteins","score":0.8640000224113464},{"id":"https://openalex.org/keywords/caenorhabditis-elegans-neurons","display_name":"Caenorhabditis elegans neurons","score":0.9580000042915344},{"id":"https://openalex.org/keywords/targeted-mutagenesis","display_name":"targeted mutagenesis","score":0.6840000152587891}],"concepts":[{"id":"https://openalex.org/C74318829","wikidata":"https://www.wikidata.org/wiki/Q36458","display_name":"Multicellular organism","level":3,"score":0.7984215617179871},{"id":"https://openalex.org/C54458228","wikidata":"https://www.wikidata.org/wiki/Q237218","display_name":"RNA splicing","level":4,"score":0.623680830001831},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.541680634021759},{"id":"https://openalex.org/C161733203","wikidata":"https://www.wikidata.org/wiki/Q911828","display_name":"Reporter gene","level":4,"score":0.5184049606323242},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.512822687625885},{"id":"https://openalex.org/C194583182","wikidata":"https://www.wikidata.org/wiki/Q21114084","display_name":"Alternative splicing","level":4,"score":0.4785400331020355},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.45893263816833496},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.40162983536720276},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3140343427658081},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.23045381903648376},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.18749195337295532},{"id":"https://openalex.org/C36823959","wikidata":"https://www.wikidata.org/wiki/Q373027","display_name":"Exon","level":3,"score":0.08763393759727478},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004742","descriptor_name":"Enhancer Elements, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007438","descriptor_name":"Introns","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D009474","descriptor_name":"Neurons","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016601","descriptor_name":"RNA-Binding Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017173","descriptor_name":"Caenorhabditis elegans","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D017398","descriptor_name":"Alternative Splicing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D017930","descriptor_name":"Genes, Reporter","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D032342","descriptor_name":"Muscle Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D037941","descriptor_name":"Silencer Elements, Transcriptional","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059372","descriptor_name":"Nucleotide Motifs","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1093/nar/gkag451","doi":"https://doi.org/10.1093/nar/gkag451","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkag451","pdf_url":null,"source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"doi:10.1101/2025.08.16.670696","doi":"https://doi.org/10.1101/2025.08.16.670696","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2025.08.16.670696","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2025/08/19/2025.08.16.670696.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nd","license_id":"https://openalex.org/licenses/cc-by-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:42179040","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42179040","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:13199693","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13199693/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Res","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1093/nar/gkag451","doi":"https://doi.org/10.1093/nar/gkag451","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkag451","pdf_url":null,"source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2399786665","display_name":"The mechanisms, function, and evolution of neuronal alternative splicing in Caenorhabditis species","funder_award_id":"rgpin-2017-06573","funder_id":"https://openalex.org/F4320334593","funder_display_name":"Natural Sciences and Engineering Research Council of Canada"},{"id":"https://openalex.org/G4442291104","display_name":null,"funder_award_id":"180365","funder_id":"https://openalex.org/F4320334506","funder_display_name":"Canadian Institutes of Health Research"},{"id":"https://openalex.org/G5894058321","display_name":null,"funder_award_id":"156300","funder_id":"https://openalex.org/F4320334506","funder_display_name":"Canadian Institutes of Health Research"}],"funders":[{"id":"https://openalex.org/F4320334506","display_name":"Canadian Institutes of Health Research","ror":"https://ror.org/01gavpb45"},{"id":"https://openalex.org/F4320334593","display_name":"Natural Sciences and Engineering Research Council of Canada","ror":"https://ror.org/01h531d29"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4413385803.pdf","grobid_xml":"https://content.openalex.org/works/W4413385803.grobid-xml"},"referenced_works_count":158,"referenced_works":["https://openalex.org/W1488980960","https://openalex.org/W1944127002","https://openalex.org/W1963918734","https://openalex.org/W1968849815","https://openalex.org/W1978883675","https://openalex.org/W1991703748","https://openalex.org/W1992451058","https://openalex.org/W1997158110","https://openalex.org/W2005609509","https://openalex.org/W2007526989","https://openalex.org/W2008807546","https://openalex.org/W2011920499","https://openalex.org/W2015646405","https://openalex.org/W2015809578","https://openalex.org/W2020766456","https://openalex.org/W2023648355","https://openalex.org/W2029979788","https://openalex.org/W2034691490","https://openalex.org/W2037367916","https://openalex.org/W2038921819","https://openalex.org/W2040148139","https://openalex.org/W2040481343","https://openalex.org/W2041677964","https://openalex.org/W2041922277","https://openalex.org/W2043829280","https://openalex.org/W2056764302","https://openalex.org/W2059230843","https://openalex.org/W2060297895","https://openalex.org/W2064350570","https://openalex.org/W2066665143","https://openalex.org/W2067791724","https://openalex.org/W2077845478","https://openalex.org/W2078108088","https://openalex.org/W2088536227","https://openalex.org/W2090521141","https://openalex.org/W2093506762","https://openalex.org/W2095998476","https://openalex.org/W2096236346","https://openalex.org/W2100668965","https://openalex.org/W2102046311","https://openalex.org/W2102337459","https://openalex.org/W2102619694","https://openalex.org/W2103534177","https://openalex.org/W2103777723","https://openalex.org/W2105694586","https://openalex.org/W2106882534","https://openalex.org/W2109936378","https://openalex.org/W2113956516","https://openalex.org/W2116911290","https://openalex.org/W2118494021","https://openalex.org/W2122163057","https://openalex.org/W2126846097","https://openalex.org/W2131187502","https://openalex.org/W2136696605","https://openalex.org/W2137351340","https://openalex.org/W2139312181","https://openalex.org/W2139480055","https://openalex.org/W2140952049","https://openalex.org/W2142676859","https://openalex.org/W2145510868","https://openalex.org/W2147842901","https://openalex.org/W2148542126","https://openalex.org/W2151462466","https://openalex.org/W2151616152","https://openalex.org/W2153562609","https://openalex.org/W2153833484","https://openalex.org/W2154565932","https://openalex.org/W2154730237","https://openalex.org/W2158020008","https://openalex.org/W2159686118","https://openalex.org/W2165954869","https://openalex.org/W2166900174","https://openalex.org/W2167279371","https://openalex.org/W2170074416","https://openalex.org/W2174355534","https://openalex.org/W2179282661","https://openalex.org/W2275300015","https://openalex.org/W2275450346","https://openalex.org/W2300235659","https://openalex.org/W2402519263","https://openalex.org/W2413854329","https://openalex.org/W2415174329","https://openalex.org/W2460850269","https://openalex.org/W2464779428","https://openalex.org/W2515399590","https://openalex.org/W2747545374","https://openalex.org/W2749613778","https://openalex.org/W2751583588","https://openalex.org/W2767275417","https://openalex.org/W2767345597","https://openalex.org/W2772439186","https://openalex.org/W2805745501","https://openalex.org/W2889059385","https://openalex.org/W2909466649","https://openalex.org/W2919897246","https://openalex.org/W2950993016","https://openalex.org/W2961228863","https://openalex.org/W2963836763","https://openalex.org/W2972184354","https://openalex.org/W2979636931","https://openalex.org/W2980949872","https://openalex.org/W2988396470","https://openalex.org/W3006983446","https://openalex.org/W3034598984","https://openalex.org/W3090512713","https://openalex.org/W3096050955","https://openalex.org/W3114870859","https://openalex.org/W3132153836","https://openalex.org/W3133222916","https://openalex.org/W3135803502","https://openalex.org/W3180956051","https://openalex.org/W3187843892","https://openalex.org/W3202118355","https://openalex.org/W3203180787","https://openalex.org/W4200017269","https://openalex.org/W4206315232","https://openalex.org/W4206761551","https://openalex.org/W4210267605","https://openalex.org/W4220731393","https://openalex.org/W4221102787","https://openalex.org/W4225981439","https://openalex.org/W4281741958","https://openalex.org/W4282958555","https://openalex.org/W4295009037","https://openalex.org/W4295513676","https://openalex.org/W4315465090","https://openalex.org/W4315779271","https://openalex.org/W4361286211","https://openalex.org/W4364381330","https://openalex.org/W4376871066","https://openalex.org/W4385449623","https://openalex.org/W4385620410","https://openalex.org/W4387303364","https://openalex.org/W4387362445","https://openalex.org/W4387935443","https://openalex.org/W4388035024","https://openalex.org/W4390918072","https://openalex.org/W4392155646","https://openalex.org/W4392968897","https://openalex.org/W4401685629","https://openalex.org/W4403090369","https://openalex.org/W4404241566","https://openalex.org/W4406385639","https://openalex.org/W4406951711","https://openalex.org/W4407913745","https://openalex.org/W4408139723","https://openalex.org/W4408213332","https://openalex.org/W4408298126","https://openalex.org/W4409292568","https://openalex.org/W4409348451","https://openalex.org/W4410447208","https://openalex.org/W4410594806","https://openalex.org/W4411192972","https://openalex.org/W4412413559","https://openalex.org/W4412707885","https://openalex.org/W6893368469","https://openalex.org/W6949985536","https://openalex.org/W7118166689"],"related_works":["https://openalex.org/W2079643255","https://openalex.org/W1982169734","https://openalex.org/W1775564965","https://openalex.org/W7096377182","https://openalex.org/W1992451058","https://openalex.org/W2121816001","https://openalex.org/W2116185451","https://openalex.org/W4412631489","https://openalex.org/W2164194877","https://openalex.org/W2117477756"],"abstract_inverted_index":{"Introns":[0],"play":[1],"a":[2,169,183,197],"critical":[3],"role":[4,188],"in":[5,43,56,62,89,192],"regulating":[6,87],"alternative":[7,35,127],"splicing.":[8,178],"However,":[9],"identifying":[10,172],"functional":[11,136],"intronic":[12,72,140,166,190],"motifs":[13,76,103,111,121],"is":[14],"challenging":[15],"due":[16],"to":[17,49,69,122,185],"their":[18,47,130,135],"short":[19],"and":[20,66,74,99,109],"degenerate":[21],"sequence":[22],"composition.":[23],"Massively":[24],"parallel":[25],"reporter":[26,59],"assays":[27,60],"have":[28],"provided":[29],"insights":[30],"into":[31],"cis-regulatory":[32,174],"logic":[33],"governing":[34],"splicing,":[36],"but":[37],"these":[38,90,147,154],"approaches":[39],"are":[40],"generally":[41],"performed":[42,161],"cell":[44],"culture,":[45],"limiting":[46],"ability":[48],"capture":[50],"tissue-specific":[51,193],"contexts.":[52],"Here,":[53],"we":[54,160],"implemented":[55],"vivo":[57],"parallelized":[58],"(PRA)":[61],"Caenorhabditis":[63],"elegans":[64],"neurons":[65],"muscle":[67],"cells":[68],"screen":[70],"for":[71],"enhancer":[73],"silencer":[75],"among":[77],"thousands":[78],"of":[79,146,150,153,164,176,189],"randomized":[80],"sequences.":[81],"We":[82,92],"identified":[83],"nearly":[84],"200":[85],"sequences":[86,155],"splicing":[88,194],"tissues.":[91],"uncovered":[93],"core":[94],"sub-sequences":[95],"with":[96,112],"tissue-biased":[97,126],"enhancing":[98],"silencing":[100],"activity,":[101],"including":[102],"recognized":[104],"by":[105,156],"well-characterized":[106],"RNA-binding":[107,157],"proteins,":[108],"unmapped":[110],"no":[113],"obvious":[114],"cognate":[115],"binding":[116],"protein.":[117],"Mapping":[118],"our":[119,180],"PRA-derived":[120,165],"native":[123],"introns":[124],"flanking":[125,168],"exons":[128],"revealed":[129],"conservation":[131],"across":[132],"nematodes,":[133],"supporting":[134],"relevance.":[137],"Additionally,":[138],"individual":[139],"regions":[141,191],"frequently":[142],"contained":[143],"diverse":[144],"combinations":[145],"motifs,":[148],"indicative":[149],"complex":[151],"engagement":[152],"proteins.":[158],"Finally,":[159],"targeted":[162],"mutagenesis":[163],"enhancers":[167],"neuronal":[170],"microexon,":[171],"key":[173],"determinants":[175],"microexon":[177],"Together,":[179],"study":[181],"provides":[182],"framework":[184],"explore":[186],"the":[187],"regulation":[195],"within":[196],"multicellular":[198],"organism.":[199]},"counts_by_year":[],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2025-10-10T00:00:00"}
