{"id":"https://openalex.org/W4413148317","doi":"https://doi.org/10.1016/j.xgen.2026.101302","title":"Compound delivery of eVLPs enhances prime editing for targeted genome engineering and high-throughput screening","display_name":"Compound delivery of eVLPs enhances prime editing for targeted genome engineering and high-throughput screening","publication_year":2026,"publication_date":"2026-07-14","ids":{"openalex":"https://openalex.org/W4413148317","doi":"https://doi.org/10.1016/j.xgen.2026.101302","pmid":"https://pubmed.ncbi.nlm.nih.gov/42447864","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/13477025"},"language":"en","primary_location":{"id":"doi:10.1016/j.xgen.2026.101302","doi":"https://doi.org/10.1016/j.xgen.2026.101302","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xgen.2026.101302","pdf_url":null,"source":{"id":"https://openalex.org/S4210194377","display_name":"Cell Genomics","issn_l":"2666-979X","issn":["2666-979X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell Genomics","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W4413148317"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1016/j.xgen.2026.101302","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5030724031","display_name":"Jethro Langley","orcid":"https://orcid.org/0000-0002-7644-4631","observed_orcids":["https://orcid.org/0000-0002-7644-4631"]},"institutions":[{"id":"https://openalex.org/I168635309","display_name":"University of Calgary","ror":"https://ror.org/03yjb2x39","country_code":"CA","type":"education","lineage":["https://openalex.org/I168635309"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Jethro Langley","raw_affiliation_strings":["The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Robson DNA Science Centre, Calgary, AB T2N 1N4, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada","Arnie Charbonneau Cancer Institute","Robson DNA Science Centre","The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Robson DNA Science Centre, Calgary, AB T2N 1N4, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada","institution_ids":["https://openalex.org/I168635309"]},{"raw_affiliation_string":"Arnie Charbonneau Cancer Institute","institution_ids":[]},{"raw_affiliation_string":"Robson DNA Science Centre","institution_ids":[]},{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada","institution_ids":["https://openalex.org/I168635309"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043439701","display_name":"Lou Baudrier","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I168635309","display_name":"University of Calgary","ror":"https://ror.org/03yjb2x39","country_code":"CA","type":"education","lineage":["https://openalex.org/I168635309"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Lou Baudrier","raw_affiliation_strings":["The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Robson DNA Science Centre, Calgary, AB T2N 1N4, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada","Arnie Charbonneau Cancer Institute","Robson DNA Science Centre","The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Robson DNA Science Centre, Calgary, AB T2N 1N4, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada","institution_ids":["https://openalex.org/I168635309"]},{"raw_affiliation_string":"Arnie Charbonneau Cancer Institute","institution_ids":[]},{"raw_affiliation_string":"Robson DNA Science Centre","institution_ids":[]},{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada","institution_ids":["https://openalex.org/I168635309"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084885800","display_name":"J.E. Curry","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I168635309","display_name":"University of Calgary","ror":"https://ror.org/03yjb2x39","country_code":"CA","type":"education","lineage":["https://openalex.org/I168635309"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Jada Curry","raw_affiliation_strings":["The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Robson DNA Science Centre, Calgary, AB T2N 1N4, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada","Arnie Charbonneau Cancer Institute","Robson DNA Science Centre","The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Robson DNA Science Centre, Calgary, AB T2N 1N4, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada","institution_ids":["https://openalex.org/I168635309"]},{"raw_affiliation_string":"Arnie Charbonneau Cancer Institute","institution_ids":[]},{"raw_affiliation_string":"Robson DNA Science Centre","institution_ids":[]},{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada","institution_ids":["https://openalex.org/I168635309"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089143806","display_name":"Kiran Narta","orcid":"https://orcid.org/0000-0002-0645-6581","observed_orcids":["https://orcid.org/0000-0002-0645-6581"]},"institutions":[{"id":"https://openalex.org/I168635309","display_name":"University of Calgary","ror":"https://ror.org/03yjb2x39","country_code":"CA","type":"education","lineage":["https://openalex.org/I168635309"]},{"id":"https://openalex.org/I4389425471","display_name":"Alberta Children's Hospital Research Institute","ror":"https://ror.org/00gmyvv50","country_code":"CA","type":"facility","lineage":["https://openalex.org/I168635309","https://openalex.org/I4389425471"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Kiran Narta","raw_affiliation_strings":["The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Robson DNA Science Centre, Calgary, AB T2N 1N4, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada; Alberta Children's Hospital Research Institute, Calgary, AB, Canada","Alberta Children's Hospital Research Institute, Calgary, AB, Canada","Arnie Charbonneau Cancer Institute","Robson DNA Science Centre","The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Robson DNA Science Centre, Calgary, AB T2N 1N4, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada; Alberta Children's Hospital Research Institute, Calgary, AB, Canada","institution_ids":["https://openalex.org/I168635309","https://openalex.org/I4389425471"]},{"raw_affiliation_string":"Alberta Children's Hospital Research Institute, Calgary, AB, Canada","institution_ids":["https://openalex.org/I4389425471"]},{"raw_affiliation_string":"Arnie Charbonneau Cancer Institute","institution_ids":[]},{"raw_affiliation_string":"Robson DNA Science Centre","institution_ids":[]},{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada","institution_ids":["https://openalex.org/I168635309"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5041082034","display_name":"Hayley M. Todesco","orcid":"https://orcid.org/0000-0002-6905-3667","observed_orcids":["https://orcid.org/0000-0002-6905-3667"]},"institutions":[{"id":"https://openalex.org/I168635309","display_name":"University of Calgary","ror":"https://ror.org/03yjb2x39","country_code":"CA","type":"education","lineage":["https://openalex.org/I168635309"]},{"id":"https://openalex.org/I4389425471","display_name":"Alberta Children's Hospital Research Institute","ror":"https://ror.org/00gmyvv50","country_code":"CA","type":"facility","lineage":["https://openalex.org/I168635309","https://openalex.org/I4389425471"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Hayley M. Todesco","raw_affiliation_strings":["The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada; Alberta Children's Hospital Research Institute, Calgary, AB, Canada; Snyder Institute for Chronic Disease, Calgary, AB, Canada; Department of Microbiology, Immunology and Infectious Disease, Calgary, AB, Canada","Alberta Children's Hospital Research Institute, Calgary, AB, Canada","Arnie Charbonneau Cancer Institute","Snyder Institute for Chronic Disease, Calgary, AB, Canada","The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada; Alberta Children's Hospital Research Institute, Calgary, AB, Canada; Snyder Institute for Chronic Disease, Calgary, AB, Canada; Department of Microbiology, Immunology and Infectious Disease, Calgary, AB, Canada","institution_ids":["https://openalex.org/I168635309","https://openalex.org/I4389425471"]},{"raw_affiliation_string":"Alberta Children's Hospital Research Institute, Calgary, AB, Canada","institution_ids":["https://openalex.org/I4389425471"]},{"raw_affiliation_string":"Arnie Charbonneau Cancer Institute","institution_ids":[]},{"raw_affiliation_string":"Snyder Institute for Chronic Disease, Calgary, AB, Canada","institution_ids":[]},{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada","institution_ids":["https://openalex.org/I168635309"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078116896","display_name":"Kyle G. Potts","orcid":"https://orcid.org/0000-0002-4991-6402","observed_orcids":["https://orcid.org/0000-0002-4991-6402"]},"institutions":[{"id":"https://openalex.org/I168635309","display_name":"University of Calgary","ror":"https://ror.org/03yjb2x39","country_code":"CA","type":"education","lineage":["https://openalex.org/I168635309"]},{"id":"https://openalex.org/I4389425471","display_name":"Alberta Children's Hospital Research Institute","ror":"https://ror.org/00gmyvv50","country_code":"CA","type":"facility","lineage":["https://openalex.org/I168635309","https://openalex.org/I4389425471"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Kyle Potts","raw_affiliation_strings":["The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada; Alberta Children's Hospital Research Institute, Calgary, AB, Canada; Snyder Institute for Chronic Disease, Calgary, AB, Canada; Department of Microbiology, Immunology and Infectious Disease, Calgary, AB, Canada","Alberta Children's Hospital Research Institute, Calgary, AB, Canada","Arnie Charbonneau Cancer Institute","Snyder Institute for Chronic Disease, Calgary, AB, Canada","The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada; Alberta Children's Hospital Research Institute, Calgary, AB, Canada; Snyder Institute for Chronic Disease, Calgary, AB, Canada; Department of Microbiology, Immunology and Infectious Disease, Calgary, AB, Canada","institution_ids":["https://openalex.org/I168635309","https://openalex.org/I4389425471"]},{"raw_affiliation_string":"Alberta Children's Hospital Research Institute, Calgary, AB, Canada","institution_ids":["https://openalex.org/I4389425471"]},{"raw_affiliation_string":"Arnie Charbonneau Cancer Institute","institution_ids":[]},{"raw_affiliation_string":"Snyder Institute for Chronic Disease, Calgary, AB, Canada","institution_ids":[]},{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada","institution_ids":["https://openalex.org/I168635309"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5023884007","display_name":"A. Sorana Morrissy","orcid":"https://orcid.org/0000-0001-8526-562X","observed_orcids":["https://orcid.org/0000-0001-8526-562X"]},"institutions":[{"id":"https://openalex.org/I168635309","display_name":"University of Calgary","ror":"https://ror.org/03yjb2x39","country_code":"CA","type":"education","lineage":["https://openalex.org/I168635309"]},{"id":"https://openalex.org/I4389425471","display_name":"Alberta Children's Hospital Research Institute","ror":"https://ror.org/00gmyvv50","country_code":"CA","type":"facility","lineage":["https://openalex.org/I168635309","https://openalex.org/I4389425471"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Sorana Morrissy","raw_affiliation_strings":["The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Robson DNA Science Centre, Calgary, AB T2N 1N4, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada; Alberta Children's Hospital Research Institute, Calgary, AB, Canada; Cumming School of Medicine, Calgary, AB T2N 2T8, Canada","Alberta Children's Hospital Research Institute, Calgary, AB, Canada","Arnie Charbonneau Cancer Institute","Robson DNA Science Centre","The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Robson DNA Science Centre, Calgary, AB T2N 1N4, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada; Alberta Children's Hospital Research Institute, Calgary, AB, Canada; Cumming School of Medicine, Calgary, AB T2N 2T8, Canada","institution_ids":["https://openalex.org/I168635309","https://openalex.org/I4389425471"]},{"raw_affiliation_string":"Alberta Children's Hospital Research Institute, Calgary, AB, Canada","institution_ids":["https://openalex.org/I4389425471"]},{"raw_affiliation_string":"Arnie Charbonneau Cancer Institute","institution_ids":[]},{"raw_affiliation_string":"Robson DNA Science Centre","institution_ids":[]},{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada","institution_ids":["https://openalex.org/I168635309"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006500798","display_name":"Douglas J. Mahoney","orcid":"https://orcid.org/0000-0002-0642-992X","observed_orcids":["https://orcid.org/0000-0002-0642-992X"]},"institutions":[{"id":"https://openalex.org/I168635309","display_name":"University of Calgary","ror":"https://ror.org/03yjb2x39","country_code":"CA","type":"education","lineage":["https://openalex.org/I168635309"]},{"id":"https://openalex.org/I4389425471","display_name":"Alberta Children's Hospital Research Institute","ror":"https://ror.org/00gmyvv50","country_code":"CA","type":"facility","lineage":["https://openalex.org/I168635309","https://openalex.org/I4389425471"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Douglas J. Mahoney","raw_affiliation_strings":["The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada; Alberta Children's Hospital Research Institute, Calgary, AB, Canada; Snyder Institute for Chronic Disease, Calgary, AB, Canada; Department of Microbiology, Immunology and Infectious Disease, Calgary, AB, Canada; Cumming School of Medicine, Calgary, AB T2N 2T8, Canada","Alberta Children's Hospital Research Institute, Calgary, AB, Canada","Arnie Charbonneau Cancer Institute","Snyder Institute for Chronic Disease, Calgary, AB, Canada","The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada; Alberta Children's Hospital Research Institute, Calgary, AB, Canada; Snyder Institute for Chronic Disease, Calgary, AB, Canada; Department of Microbiology, Immunology and Infectious Disease, Calgary, AB, Canada; Cumming School of Medicine, Calgary, AB T2N 2T8, Canada","institution_ids":["https://openalex.org/I168635309","https://openalex.org/I4389425471"]},{"raw_affiliation_string":"Alberta Children's Hospital Research Institute, Calgary, AB, Canada","institution_ids":["https://openalex.org/I4389425471"]},{"raw_affiliation_string":"Arnie Charbonneau Cancer Institute","institution_ids":[]},{"raw_affiliation_string":"Snyder Institute for Chronic Disease, Calgary, AB, Canada","institution_ids":[]},{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada","institution_ids":["https://openalex.org/I168635309"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5108610568","display_name":"Pierre Billon","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I168635309","display_name":"University of Calgary","ror":"https://ror.org/03yjb2x39","country_code":"CA","type":"education","lineage":["https://openalex.org/I168635309"]}],"countries":["CA"],"is_corresponding":true,"raw_author_name":"Pierre Billon","raw_affiliation_strings":["The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Robson DNA Science Centre, Calgary, AB T2N 1N4, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada; Cumming School of Medicine, Calgary, AB T2N 2T8, Canada. Electronic address: pierre.billon@ucalgary.ca","Arnie Charbonneau Cancer Institute","Robson DNA Science Centre","The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, AB T2N 4N1, Canada; Robson DNA Science Centre, Calgary, AB T2N 1N4, Canada; Arnie Charbonneau Cancer Institute, Calgary, AB T2N 4Z6, Canada; Cumming School of Medicine, Calgary, AB T2N 2T8, Canada. Electronic address: pierre.billon@ucalgary.ca","institution_ids":["https://openalex.org/I168635309"]},{"raw_affiliation_string":"Arnie Charbonneau Cancer Institute","institution_ids":[]},{"raw_affiliation_string":"Robson DNA Science Centre","institution_ids":[]},{"raw_affiliation_string":"The University of Calgary, Cumming School of Medicine, Department of Biochemistry and Molecular Biology, 3330 Hospital Drive N. W., Calgary, Alberta T2N 4N, Canada","institution_ids":["https://openalex.org/I168635309"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5108610568"],"corresponding_institution_ids":["https://openalex.org/I168635309"],"apc_list":{"value":8900,"currency":"USD","value_usd":8900},"apc_paid":{"value":8900,"currency":"USD","value_usd":8900},"fwci":6.5119,"has_fulltext":true,"cited_by_count":5,"citation_normalized_percentile":{"value":0.96381276,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":97,"max":98},"biblio":{"volume":"6","issue":"8","first_page":"101302","last_page":"101302"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9907000064849854,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9907000064849854,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.006599999964237213,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/prime-editing","display_name":"prime editing","score":0.9829999804496765},{"id":"https://openalex.org/keywords/off-target-editing","display_name":"off-target editing","score":0.75},{"id":"https://openalex.org/keywords/transgene-silencing","display_name":"transgene silencing","score":0.7580000162124634},{"id":"https://openalex.org/keywords/high-throughput-functional-genomics","display_name":"high-throughput functional genomics","score":0.7400000095367432}],"concepts":[{"id":"https://openalex.org/C157764524","wikidata":"https://www.wikidata.org/wiki/Q1383412","display_name":"Throughput","level":3,"score":0.7331938743591309},{"id":"https://openalex.org/C184992742","wikidata":"https://www.wikidata.org/wiki/Q7243229","display_name":"Prime (order theory)","level":2,"score":0.698054313659668},{"id":"https://openalex.org/C51323132","wikidata":"https://www.wikidata.org/wiki/Q626251","display_name":"High-throughput screening","level":2,"score":0.586140513420105},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.4898347556591034},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.46760693192481995},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.43595874309539795},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.28617608547210693},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.1967020034790039},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.09163370728492737},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08924469351768494},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.08520624041557312},{"id":"https://openalex.org/C555944384","wikidata":"https://www.wikidata.org/wiki/Q249","display_name":"Wireless","level":2,"score":0.0},{"id":"https://openalex.org/C114614502","wikidata":"https://www.wikidata.org/wiki/Q76592","display_name":"Combinatorics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014771","descriptor_name":"Virion","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016086","descriptor_name":"Lentivirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D016159","descriptor_name":"Tumor Suppressor Protein p53","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D057166","descriptor_name":"High-Throughput Screening Assays","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D057809","descriptor_name":"HEK293 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1016/j.xgen.2026.101302","doi":"https://doi.org/10.1016/j.xgen.2026.101302","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xgen.2026.101302","pdf_url":null,"source":{"id":"https://openalex.org/S4210194377","display_name":"Cell Genomics","issn_l":"2666-979X","issn":["2666-979X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell Genomics","raw_type":"journal-article"},{"id":"doi:10.1101/2025.08.11.669692","doi":"https://doi.org/10.1101/2025.08.11.669692","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2025.08.11.669692","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmh:oai:pubmedcentral.nih.gov:13477025","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13477025/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13477025/pdf/","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell Genom","raw_type":"Text"},{"id":"pmid:42447864","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/42447864","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell genomics","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/j.xgen.2026.101302","doi":"https://doi.org/10.1016/j.xgen.2026.101302","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xgen.2026.101302","pdf_url":null,"source":{"id":"https://openalex.org/S4210194377","display_name":"Cell Genomics","issn_l":"2666-979X","issn":["2666-979X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell Genomics","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5608680069","display_name":null,"funder_award_id":"508188","funder_id":"https://openalex.org/F4320334506","funder_display_name":"Canadian Institutes of Health Research"}],"funders":[{"id":"https://openalex.org/F4320310537","display_name":"University of Calgary","ror":"https://ror.org/03yjb2x39"},{"id":"https://openalex.org/F4320313420","display_name":"Lotte and John Hecht Memorial Foundation","ror":"https://ror.org/032v4f614"},{"id":"https://openalex.org/F4320334506","display_name":"Canadian Institutes of Health Research","ror":"https://ror.org/01gavpb45"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4413148317.pdf","grobid_xml":"https://content.openalex.org/works/W4413148317.grobid-xml"},"referenced_works_count":108,"referenced_works":["https://openalex.org/W60416080","https://openalex.org/W2044433163","https://openalex.org/W2048607934","https://openalex.org/W2050870581","https://openalex.org/W2051978340","https://openalex.org/W2057040546","https://openalex.org/W2059300459","https://openalex.org/W2075230442","https://openalex.org/W2093456378","https://openalex.org/W2097933346","https://openalex.org/W2100122648","https://openalex.org/W2116534141","https://openalex.org/W2139195068","https://openalex.org/W2142630559","https://openalex.org/W2144654038","https://openalex.org/W2160051211","https://openalex.org/W2169456326","https://openalex.org/W2179438025","https://openalex.org/W2336828812","https://openalex.org/W2570621777","https://openalex.org/W2744559176","https://openalex.org/W2752453466","https://openalex.org/W2760582164","https://openalex.org/W2766599608","https://openalex.org/W2804348137","https://openalex.org/W2809042973","https://openalex.org/W2887637071","https://openalex.org/W2891731267","https://openalex.org/W2895586011","https://openalex.org/W2913088976","https://openalex.org/W2915459742","https://openalex.org/W2949383259","https://openalex.org/W2981137429","https://openalex.org/W3010674213","https://openalex.org/W3012036607","https://openalex.org/W3035764705","https://openalex.org/W3037601773","https://openalex.org/W3085175179","https://openalex.org/W3099862724","https://openalex.org/W3121977414","https://openalex.org/W3122603135","https://openalex.org/W3126160703","https://openalex.org/W3127207441","https://openalex.org/W3127333316","https://openalex.org/W3141751158","https://openalex.org/W3164457455","https://openalex.org/W3200735940","https://openalex.org/W3202931255","https://openalex.org/W3206354438","https://openalex.org/W3212971201","https://openalex.org/W4206238555","https://openalex.org/W4206390074","https://openalex.org/W4211123163","https://openalex.org/W4211219428","https://openalex.org/W4213232054","https://openalex.org/W4221083162","https://openalex.org/W4225688524","https://openalex.org/W4226280654","https://openalex.org/W4283070781","https://openalex.org/W4288732582","https://openalex.org/W4290673775","https://openalex.org/W4303199315","https://openalex.org/W4308486212","https://openalex.org/W4308770459","https://openalex.org/W4308772150","https://openalex.org/W4312195490","https://openalex.org/W4366163388","https://openalex.org/W4367311810","https://openalex.org/W4379930708","https://openalex.org/W4382515361","https://openalex.org/W4386090744","https://openalex.org/W4386528923","https://openalex.org/W4387095975","https://openalex.org/W4388532130","https://openalex.org/W4390063959","https://openalex.org/W4390670214","https://openalex.org/W4390750892","https://openalex.org/W4391406569","https://openalex.org/W4391925485","https://openalex.org/W4392166215","https://openalex.org/W4392705635","https://openalex.org/W4393902863","https://openalex.org/W4394727560","https://openalex.org/W4394759822","https://openalex.org/W4394873238","https://openalex.org/W4395675467","https://openalex.org/W4399087850","https://openalex.org/W4404252661","https://openalex.org/W4404252802","https://openalex.org/W4404333818","https://openalex.org/W4404497408","https://openalex.org/W4404824804","https://openalex.org/W4405896537","https://openalex.org/W4406061191","https://openalex.org/W4406150887","https://openalex.org/W4408243981","https://openalex.org/W4408732759","https://openalex.org/W4409190839","https://openalex.org/W4409283383","https://openalex.org/W4411575906","https://openalex.org/W4413645171","https://openalex.org/W4414947571","https://openalex.org/W4417031746","https://openalex.org/W4417310329","https://openalex.org/W7128701559","https://openalex.org/W7130701762","https://openalex.org/W7161813637","https://openalex.org/W7162026039"],"related_works":["https://openalex.org/W7202272246","https://openalex.org/W7221528009","https://openalex.org/W4390670214","https://openalex.org/W7164862100","https://openalex.org/W7212262458","https://openalex.org/W7131218237","https://openalex.org/W4409957833","https://openalex.org/W4220928625","https://openalex.org/W4409283383","https://openalex.org/W4404497408"],"abstract_inverted_index":{"Engineered":[0],"virus-like":[1],"particles":[2],"(eVLPs)":[3],"enable":[4],"transgene-free":[5],"ribonucleoprotein":[6],"delivery":[7,89],"for":[8,14,37,148],"genome":[9],"editing,":[10,76],"yet":[11],"optimized":[12],"strategies":[13],"high-throughput":[15,149],"applications":[16],"remain":[17],"unexplored.":[18],"Prime":[19],"editing":[20,46,64,97,115],"enables":[21,94],"precise":[22],"genomic":[23,68],"modifications":[24],"but":[25],"suffers":[26],"from":[27],"limited":[28],"efficiency.":[29],"Here,":[30],"we":[31],"present":[32],"PRIME-VLP":[33,56,93,111],"(Progressive":[34],"Repeated":[35],"Infections":[36],"Maximized":[38],"Editing":[39],"via":[40],"Virus-Like":[41],"Particles),":[42],"which":[43],"exploits":[44],"eVLP-mediated":[45],"kinetics":[47],"through":[48,90],"multiple":[49],"sequential":[50],"sub-saturating":[51],"transductions":[52],"at":[53],"optimal":[54],"intervals.":[55],"achieves":[57],"1.5-":[58],"to":[59,120],"2.9-fold":[60],"improvements":[61],"in":[62,141],"prime":[63,96],"efficiency":[65],"across":[66],"diverse":[67],"targets":[69],"and":[70,87,99,116],"cell":[71],"types":[72],"without":[73],"increasing":[74],"off-target":[75],"compromising":[77],"cellular":[78],"viability":[79],"or":[80],"causing":[81],"transcriptional":[82],"perturbations.":[83],"By":[84],"decoupling":[85],"pegRNA":[86],"editor":[88],"pegRNA-free":[91],"eVLPs,":[92],"pooled":[95],"screens":[98],"circumvents":[100],"transgene":[101],"silencing":[102],"limitations.":[103],"Using":[104],"a":[105],"6,000-pegRNA":[106],"library":[107],"targeting":[108],"TP53":[109,125],",":[110],"achieved":[112],"2.8-fold":[113],"higher":[114],"improved":[117],"reproducibility":[118],"compared":[119],"conventional":[121],"lentiviral":[122],"delivery,":[123],"identifying":[124],"loss-of-function":[126],"variants":[127],"conferring":[128],"Nutlin-3":[129],"resistance.":[130],"This":[131],"work":[132],"expands":[133],"the":[134],"versatility":[135],"of":[136],"eVLPs":[137],"beyond":[138],"their":[139,146],"current":[140],"vivo":[142],"therapeutic":[143],"applications,":[144],"demonstrating":[145],"promise":[147],"functional":[150],"genomics.":[151]},"counts_by_year":[{"year":2026,"cited_by_count":5}],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2025-10-10T00:00:00"}