{"id":"https://openalex.org/W4412795339","doi":"https://doi.org/10.3791/31430","title":"Fluorescence Staining of Neural Crest Cells Cultured on Hydrogels of Varying Stiffness","display_name":"Fluorescence Staining of Neural Crest Cells Cultured on Hydrogels of Varying Stiffness","publication_year":2025,"publication_date":"2025-07-31","ids":{"openalex":"https://openalex.org/W4412795339","doi":"https://doi.org/10.3791/31430"},"language":"en","primary_location":{"id":"doi:10.3791/31430","doi":"https://doi.org/10.3791/31430","is_oa":false,"landing_page_url":"https://doi.org/10.3791/31430","pdf_url":null,"source":{"id":"https://openalex.org/S7407064559","display_name":"Journal of Visualized Experiments","issn_l":"3065-5374","issn":["3065-5374"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.15470688,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10379","display_name":"Cellular Mechanics and Interactions","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10343","display_name":"Hydrogels: synthesis, properties, applications","score":0.0015999999595806003,"subfield":{"id":"https://openalex.org/subfields/1313","display_name":"Molecular Medicine"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10608","display_name":"Neurogenesis and neuroplasticity mechanisms","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2806","display_name":"Developmental Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/neural-crest-cells","display_name":"neural crest cells","score":0.9990000128746033},{"id":"https://openalex.org/keywords/hydrogels","display_name":"hydrogels","score":0.8790000081062317},{"id":"https://openalex.org/keywords/substrate-stiffness","display_name":"substrate stiffness","score":0.8209999799728394},{"id":"https://openalex.org/keywords/mechanotransduction","display_name":"mechanotransduction","score":0.7540000081062317},{"id":"https://openalex.org/keywords/cytoskeleton-organization","display_name":"cytoskeletal organization","score":0.8489999771118164},{"id":"https://openalex.org/keywords/phalloidin-staining","display_name":"phalloidin staining","score":0.9729999899864197},{"id":"https://openalex.org/keywords/cell-culture","display_name":"cell culture","score":0.3889999985694885},{"id":"https://openalex.org/keywords/fluorescence-microscopy","display_name":"fluorescence microscopy","score":0.5389999747276306}],"concepts":[{"id":"https://openalex.org/C126749454","wikidata":"https://www.wikidata.org/wiki/Q1348455","display_name":"Neural crest","level":3,"score":0.7844812870025635},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.6963129639625549},{"id":"https://openalex.org/C108586683","wikidata":"https://www.wikidata.org/wiki/Q17164826","display_name":"Self-healing hydrogels","level":2,"score":0.684540867805481},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.6420671939849854},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5041245222091675},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.4729696810245514},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4655735194683075},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4047716557979584},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.3247099220752716},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3100852370262146},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.19014135003089905},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.1279817521572113},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.12572252750396729},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.06069028377532959},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.3791/31430","doi":"https://doi.org/10.3791/31430","is_oa":false,"landing_page_url":"https://doi.org/10.3791/31430","pdf_url":null,"source":{"id":"https://openalex.org/S7407064559","display_name":"Journal of Visualized Experiments","issn_l":"3065-5374","issn":["3065-5374"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Visualized Experiments","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2038809418","https://openalex.org/W2277257395","https://openalex.org/W2485176123","https://openalex.org/W4211054287","https://openalex.org/W2891312418","https://openalex.org/W2493939882","https://openalex.org/W2503803213","https://openalex.org/W2800680006","https://openalex.org/W2379987675","https://openalex.org/W2735679541"],"abstract_inverted_index":{"Source:":[0],"Le,":[1],"T.":[2],"P.,":[3],"et":[4],"al.,":[5],"An":[6],"Optimized":[7],"O9-1/Hydrogel":[8],"System":[9],"for":[10],"Studying":[11],"Mechanical":[12],"Signals":[13],"in":[14],"Neural":[15],"Crest":[16],"Cells.":[17],"J.":[18],"Vis.":[19],"Exp.":[20],"(2021)":[21],"In":[22],"this":[23],"video,":[24],"we":[25],"describe":[26],"the":[27],"procedure":[28],"to":[29,36,50],"perform":[30],"fluorescence":[31,34],"staining":[32,39],"using":[33],"microscopy":[35],"demonstrate":[37],"phalloidin":[38],"of":[40,47],"neural":[41],"crest":[42],"cells":[43],"cultured":[44],"on":[45],"hydrogels":[46],"varying":[48],"stiffness":[49],"visualize":[51],"and":[52],"analyze":[53],"cytoskeletal":[54],"organization.":[55]},"counts_by_year":[],"updated_date":"2026-09-06T09:10:53.072986","created_date":"2025-10-10T00:00:00"}