{"id":"https://openalex.org/W4412480459","doi":"https://doi.org/10.7554/elife.105937.2","title":"C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin","display_name":"C-terminal tagging, transmembrane domain hydrophobicity, and an ER retention motif influence the secretory trafficking of the inner nuclear membrane protein emerin","publication_year":2025,"publication_date":"2025-07-16","ids":{"openalex":"https://openalex.org/W4412480459","doi":"https://doi.org/10.7554/elife.105937.2"},"language":"en","primary_location":{"id":"doi:10.7554/elife.105937.2","doi":"https://doi.org/10.7554/elife.105937.2","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.105937.2","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"eLife","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.7554/elife.105937.2","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5103119462","display_name":"Jessica Mella","orcid":"https://orcid.org/0000-0001-7541-6789","observed_orcids":["https://orcid.org/0000-0001-7541-6789"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jessica Mella","raw_affiliation_strings":["Cardiovascular Research Institute, University of California, San Francisco"],"raw_orcid":"https://orcid.org/0000-0001-7541-6789","affiliations":[{"raw_affiliation_string":"Cardiovascular Research Institute, University of California, San Francisco","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019889816","display_name":"Regan F. Volk","orcid":"https://orcid.org/0000-0001-6748-719X","observed_orcids":["https://orcid.org/0000-0001-6748-719X"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Regan Volk","raw_affiliation_strings":["Cardiovascular Research Institute, University of California, San Francisco"],"raw_orcid":"https://orcid.org/0000-0001-6748-719X","affiliations":[{"raw_affiliation_string":"Cardiovascular Research Institute, University of California, San Francisco","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065154963","display_name":"Balyn W. Zaro","orcid":"https://orcid.org/0000-0002-8938-9889","observed_orcids":["https://orcid.org/0000-0002-8938-9889"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Balyn Zaro","raw_affiliation_strings":["Cardiovascular Research Institute, University of California, San Francisco","Department of Pharmaceutical Chemistry, University of California, San Francisco"],"raw_orcid":"https://orcid.org/0000-0002-8938-9889","affiliations":[{"raw_affiliation_string":"Cardiovascular Research Institute, University of California, San Francisco","institution_ids":["https://openalex.org/I180670191"]},{"raw_affiliation_string":"Department of Pharmaceutical Chemistry, University of California, San Francisco","institution_ids":["https://openalex.org/I180670191"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5045678990","display_name":"Abigail L. Buchwalter","orcid":"https://orcid.org/0000-0001-7181-6961","observed_orcids":["https://orcid.org/0000-0001-7181-6961"]},"institutions":[{"id":"https://openalex.org/I180670191","display_name":"University of California, San Francisco","ror":"https://ror.org/043mz5j54","country_code":"US","type":"education","lineage":["https://openalex.org/I180670191"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Abigail Buchwalter","raw_affiliation_strings":["Cardiovascular Research Institute, University of California, San Francisco","Department of Physiology, University of California, San Francisco"],"raw_orcid":"https://orcid.org/0000-0001-7181-6961","affiliations":[{"raw_affiliation_string":"Cardiovascular Research Institute, University of California, San Francisco","institution_ids":["https://openalex.org/I180670191"]},{"raw_affiliation_string":"Department of Physiology, University of California, San Francisco","institution_ids":["https://openalex.org/I180670191"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I180670191"],"apc_list":{"value":3000,"currency":"USD","value_usd":2500},"apc_paid":{"value":3000,"currency":"USD","value_usd":2500},"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11841","display_name":"Nuclear Structure and Function","score":0.9868000149726868,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11841","display_name":"Nuclear Structure and Function","score":0.9868000149726868,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.009100000374019146,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11650","display_name":"Skin and Cellular Biology Research","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/emerin","display_name":"emerin","score":1.0},{"id":"https://openalex.org/keywords/inner-nuclear-membrane","display_name":"inner nuclear membrane","score":0.9279999732971191},{"id":"https://openalex.org/keywords/secretory-trafficking","display_name":"secretory trafficking","score":0.8289999961853027},{"id":"https://openalex.org/keywords/lysosomal-degradation","display_name":"lysosomal degradation","score":0.7919999957084656},{"id":"https://openalex.org/keywords/c-terminal-tagging","display_name":"C-terminal tagging","score":0.7149999737739563},{"id":"https://openalex.org/keywords/er-retention-motif","display_name":"ER retention motif","score":0.8980000019073486},{"id":"https://openalex.org/keywords/protein-localization","display_name":"protein localization","score":0.5529999732971191},{"id":"https://openalex.org/keywords/endoplasmic-reticulum","display_name":"endoplasmic reticulum","score":0.8560000061988831}],"concepts":[{"id":"https://openalex.org/C2781023310","wikidata":"https://www.wikidata.org/wiki/Q905741","display_name":"Emerin","level":5,"score":0.9026468992233276},{"id":"https://openalex.org/C32276052","wikidata":"https://www.wikidata.org/wiki/Q908349","display_name":"Motif (music)","level":2,"score":0.6259022355079651},{"id":"https://openalex.org/C118892022","wikidata":"https://www.wikidata.org/wiki/Q7834587","display_name":"Transmembrane domain","level":3,"score":0.6140134334564209},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.609602689743042},{"id":"https://openalex.org/C24530287","wikidata":"https://www.wikidata.org/wiki/Q424204","display_name":"Transmembrane protein","level":3,"score":0.5835387706756592},{"id":"https://openalex.org/C65871279","wikidata":"https://www.wikidata.org/wiki/Q42884945","display_name":"Inner membrane","level":3,"score":0.5251295566558838},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5113696455955505},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.471100389957428},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.44636109471321106},{"id":"https://openalex.org/C57711820","wikidata":"https://www.wikidata.org/wiki/Q310425","display_name":"C-terminus","level":3,"score":0.4331490993499756},{"id":"https://openalex.org/C2779664074","wikidata":"https://www.wikidata.org/wiki/Q3518405","display_name":"Terminal (telecommunication)","level":2,"score":0.43176668882369995},{"id":"https://openalex.org/C144647389","wikidata":"https://www.wikidata.org/wiki/Q423042","display_name":"Membrane protein","level":3,"score":0.4271129369735718},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.28197407722473145},{"id":"https://openalex.org/C29512474","wikidata":"https://www.wikidata.org/wiki/Q16860021","display_name":"Nuclear protein","level":4,"score":0.2585794925689697},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.24969425797462463},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.1214185357093811},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.11391916871070862},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.10752969980239868},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.0664883553981781},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.058924853801727295},{"id":"https://openalex.org/C76155785","wikidata":"https://www.wikidata.org/wiki/Q418","display_name":"Telecommunications","level":1,"score":0.0},{"id":"https://openalex.org/C24890656","wikidata":"https://www.wikidata.org/wiki/Q82811","display_name":"Acoustics","level":1,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.7554/elife.105937.2","doi":"https://doi.org/10.7554/elife.105937.2","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.105937.2","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"eLife","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.7554/elife.105937.2","doi":"https://doi.org/10.7554/elife.105937.2","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.105937.2","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"eLife","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5246730260","display_name":null,"funder_award_id":"F31HL170757","funder_id":"https://openalex.org/F4320320334","funder_display_name":"National Heart and Lung Institute"}],"funders":[{"id":"https://openalex.org/F4320315474","display_name":"Chan Zuckerberg Initiative","ror":"https://ror.org/02qenvm24"},{"id":"https://openalex.org/F4320320334","display_name":"National Heart and Lung Institute","ror":"https://ror.org/041kmwe10"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":40,"referenced_works":["https://openalex.org/W1522741331","https://openalex.org/W1888731578","https://openalex.org/W1901789611","https://openalex.org/W1912136070","https://openalex.org/W1988629688","https://openalex.org/W1991868625","https://openalex.org/W1996478334","https://openalex.org/W1999354803","https://openalex.org/W2036559009","https://openalex.org/W2052496852","https://openalex.org/W2056204193","https://openalex.org/W2066015352","https://openalex.org/W2070136749","https://openalex.org/W2086069231","https://openalex.org/W2092243418","https://openalex.org/W2108708484","https://openalex.org/W2144337984","https://openalex.org/W2144938444","https://openalex.org/W2145488605","https://openalex.org/W2151390847","https://openalex.org/W2152031903","https://openalex.org/W2172082162","https://openalex.org/W2242039608","https://openalex.org/W2282178641","https://openalex.org/W2312987066","https://openalex.org/W2485148075","https://openalex.org/W2611776924","https://openalex.org/W2767831246","https://openalex.org/W2950512597","https://openalex.org/W2951084365","https://openalex.org/W2980207167","https://openalex.org/W3022890385","https://openalex.org/W3084158527","https://openalex.org/W3112854071","https://openalex.org/W3197693442","https://openalex.org/W4212841047","https://openalex.org/W4283796562","https://openalex.org/W4383998628","https://openalex.org/W6782853382","https://openalex.org/W6787765760"],"related_works":["https://openalex.org/W2606881397","https://openalex.org/W1950573144","https://openalex.org/W2980207167","https://openalex.org/W763708196","https://openalex.org/W2054334357","https://openalex.org/W2282178641","https://openalex.org/W2910063466","https://openalex.org/W2747560187","https://openalex.org/W2602185923","https://openalex.org/W86630917"],"abstract_inverted_index":{"Abstract":[0],"The":[1],"inner":[2],"nuclear":[3],"membrane":[4],"(INM),":[5],"a":[6,101,114],"subdomain":[7],"of":[8,15,144],"the":[9,19,31,37,94,105],"endoplasmic":[10],"reticulum":[11],"(ER),":[12],"sequesters":[13],"hundreds":[14],"transmembrane":[16,89],"proteins":[17],"within":[18],"nucleus.":[20],"We":[21],"previously":[22],"found":[23],"that":[24,54,75,119],"one":[25],"INM":[26,32,146],"protein,":[27],"emerin,":[28],"can":[29],"evade":[30],"by":[33,126],"secretory":[34,59],"transport":[35],"to":[36,50,80,93],"lysosome,":[38,95],"where":[39],"it":[40],"is":[41,78],"degraded.":[42],"In":[43,110],"this":[44,108],"work,":[45],"we":[46,72,112],"used":[47],"targeted":[48],"mutagenesis":[49],"identify":[51,113],"intrinsic":[52],"sequences":[53,64],"promote":[55],"or":[56],"inhibit":[57],"emerin\u2019s":[58,76,85],"trafficking.":[60],"By":[61],"manipulating":[62],"these":[63],"across":[65],"several":[66],"tag":[67,103],"and":[68,122,140],"expression":[69],"level":[70],"combinations,":[71],"now":[73],"find":[74],"localization":[77],"sensitive":[79],"C-terminal":[81,88],"GFP":[82,102],"tagging.":[83],"While":[84],"long,":[86],"hydrophobic":[87],"domain":[90],"facilitates":[91],"trafficking":[92],"extending":[96],"its":[97,128],"lumenal":[98],"terminus":[99],"with":[100],"biases":[104],"protein":[106,147],"toward":[107],"pathway.":[109],"contrast,":[111],"conserved":[115],"ER":[116],"retention":[117],"sequence":[118],"stabilizes":[120],"N-":[121],"C-terminally":[123],"tagged":[124],"emerin":[125],"limiting":[127],"lysosomal":[129],"flux.":[130],"These":[131],"findings":[132],"underscore":[133],"long-":[134],"standing":[135],"concerns":[136],"about":[137],"tagging":[138],"artifacts":[139],"reveal":[141],"novel":[142],"determinants":[143],"tail-anchored":[145],"targeting.":[148]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2025-10-10T00:00:00"}