{"id":"https://openalex.org/W4412377971","doi":"https://doi.org/10.21769/bioprotoc.5398","title":"ZnCl2 Precipitation-Assisted Sample Preparation for Proteomic Analysis","display_name":"ZnCl2 Precipitation-Assisted Sample Preparation for Proteomic Analysis","publication_year":2025,"publication_date":"2025-01-01","ids":{"openalex":"https://openalex.org/W4412377971","doi":"https://doi.org/10.21769/bioprotoc.5398","pmid":"https://pubmed.ncbi.nlm.nih.gov/40741397"},"language":"en","primary_location":{"id":"doi:10.21769/bioprotoc.5398","doi":"https://doi.org/10.21769/bioprotoc.5398","is_oa":true,"landing_page_url":"https://doi.org/10.21769/bioprotoc.5398","pdf_url":null,"source":{"id":"https://openalex.org/S4210179406","display_name":"BIO-PROTOCOL","issn_l":"2331-8325","issn":["2331-8325"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320252","host_organization_name":"American Academy of Arts and Sciences","host_organization_lineage":["https://openalex.org/P4310320252"],"host_organization_lineage_names":["American Academy of Arts and Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BIO-PROTOCOL","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://doi.org/10.21769/bioprotoc.5398","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113370953","display_name":"Qiqing He","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I3045169105","display_name":"Southern University of Science and Technology","ror":"https://ror.org/049tv2d57","country_code":"CN","type":"education","lineage":["https://openalex.org/I3045169105"]},{"id":"https://openalex.org/I4210149540","display_name":"Beijing Proteome Research Center","ror":"https://ror.org/05pp5b412","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210149540"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qiqing He","raw_affiliation_strings":["Department of Chemistry, School of Science, Southern University of Science and Technology, Shenzhen, China","International Academy of Phronesis Medicine, Guangzhou, China","State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, School of Science, Southern University of Science and Technology, Shenzhen, China","institution_ids":["https://openalex.org/I3045169105"]},{"raw_affiliation_string":"International Academy of Phronesis Medicine, Guangzhou, China","institution_ids":[]},{"raw_affiliation_string":"State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, China","institution_ids":["https://openalex.org/I4210149540"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Qingjing Chen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210103346","display_name":"Nanfang Hospital","ror":"https://ror.org/01eq10738","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210103346"]},{"id":"https://openalex.org/I4210149540","display_name":"Beijing Proteome Research Center","ror":"https://ror.org/05pp5b412","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210149540"]},{"id":"https://openalex.org/I58200834","display_name":"Southern Medical University","ror":"https://ror.org/01vjw4z39","country_code":"CN","type":"education","lineage":["https://openalex.org/I58200834"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qingjing Chen","raw_affiliation_strings":["Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, China","International Academy of Phronesis Medicine, Guangzhou, China","State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, China","institution_ids":["https://openalex.org/I4210103346","https://openalex.org/I58200834"]},{"raw_affiliation_string":"International Academy of Phronesis Medicine, Guangzhou, China","institution_ids":[]},{"raw_affiliation_string":"State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, China","institution_ids":["https://openalex.org/I4210149540"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Dongxue Wang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I200296433","display_name":"Chinese Academy of Medical Sciences & Peking Union Medical College","ror":"https://ror.org/02drdmm93","country_code":"CN","type":"education","lineage":["https://openalex.org/I200296433"]},{"id":"https://openalex.org/I4210149540","display_name":"Beijing Proteome Research Center","ror":"https://ror.org/05pp5b412","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210149540"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Dongxue Wang","raw_affiliation_strings":["International Academy of Phronesis Medicine, Guangzhou, China","Research Unit of Proteomics Driven Cancer Precision Medicine, Chinese Academy of Medical Sciences, Beijing, China","State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"International Academy of Phronesis Medicine, Guangzhou, China","institution_ids":[]},{"raw_affiliation_string":"Research Unit of Proteomics Driven Cancer Precision Medicine, Chinese Academy of Medical Sciences, Beijing, China","institution_ids":["https://openalex.org/I200296433"]},{"raw_affiliation_string":"State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, China","institution_ids":["https://openalex.org/I4210149540"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5091436402","display_name":"Fuchu He","orcid":"https://orcid.org/0000-0002-8571-2351","observed_orcids":["https://orcid.org/0000-0002-8571-2351"]},"institutions":[{"id":"https://openalex.org/I200296433","display_name":"Chinese Academy of Medical Sciences & Peking Union Medical College","ror":"https://ror.org/02drdmm93","country_code":"CN","type":"education","lineage":["https://openalex.org/I200296433"]},{"id":"https://openalex.org/I3045169105","display_name":"Southern University of Science and Technology","ror":"https://ror.org/049tv2d57","country_code":"CN","type":"education","lineage":["https://openalex.org/I3045169105"]},{"id":"https://openalex.org/I4210103346","display_name":"Nanfang Hospital","ror":"https://ror.org/01eq10738","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210103346"]},{"id":"https://openalex.org/I4210149540","display_name":"Beijing Proteome Research Center","ror":"https://ror.org/05pp5b412","country_code":"CN","type":"facility","lineage":["https://openalex.org/I4210149540"]},{"id":"https://openalex.org/I58200834","display_name":"Southern Medical University","ror":"https://ror.org/01vjw4z39","country_code":"CN","type":"education","lineage":["https://openalex.org/I58200834"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Fuchu He","raw_affiliation_strings":["Department of Chemistry, School of Science, Southern University of Science and Technology, Shenzhen, China","Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, China","International Academy of Phronesis Medicine, Guangzhou, China","Research Unit of Proteomics Driven Cancer Precision Medicine, Chinese Academy of Medical Sciences, Beijing, China","State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, School of Science, Southern University of Science and Technology, Shenzhen, China","institution_ids":["https://openalex.org/I3045169105"]},{"raw_affiliation_string":"Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, Guangzhou, China","institution_ids":["https://openalex.org/I4210103346","https://openalex.org/I58200834"]},{"raw_affiliation_string":"International Academy of Phronesis Medicine, Guangzhou, China","institution_ids":[]},{"raw_affiliation_string":"Research Unit of Proteomics Driven Cancer Precision Medicine, Chinese Academy of Medical Sciences, Beijing, China","institution_ids":["https://openalex.org/I200296433"]},{"raw_affiliation_string":"State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, China","institution_ids":["https://openalex.org/I4210149540"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":5,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.07810277,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"15","issue":"1376","first_page":"e5398","last_page":"e5398"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10519","display_name":"Advanced Proteomics Techniques and Applications","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12867","display_name":"Advanced Biosensing Techniques and Applications","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/zasp","display_name":"ZASP","score":0.7680000066757202},{"id":"https://openalex.org/keywords/proteomics-sample-preparation","display_name":"proteomics sample preparation","score":0.7559999823570251},{"id":"https://openalex.org/keywords/detergent-removal","display_name":"detergent removal","score":0.6570000052452087},{"id":"https://openalex.org/keywords/tryptic-digestion","display_name":"trypsin digestion","score":0.8100000023841858},{"id":"https://openalex.org/keywords/protein-recovery","display_name":"protein recovery","score":0.6700000166893005},{"id":"https://openalex.org/keywords/mice","display_name":"mice","score":0.46799999475479126},{"id":"https://openalex.org/keywords/cost-effective-sample-preparation","display_name":"cost-effective sample preparation","score":0.6489999890327454},{"id":"https://openalex.org/keywords/formalin-fixed-paraffin-embedded-tissue","display_name":"formalin-fixed paraffin-embedded tissue","score":0.8309999704360962}],"concepts":[{"id":"https://openalex.org/C2778576202","wikidata":"https://www.wikidata.org/wiki/Q2540164","display_name":"Sample preparation","level":2,"score":0.5504067540168762},{"id":"https://openalex.org/C107054158","wikidata":"https://www.wikidata.org/wiki/Q25257","display_name":"Precipitation","level":2,"score":0.5458042025566101},{"id":"https://openalex.org/C198531522","wikidata":"https://www.wikidata.org/wiki/Q485146","display_name":"Sample (material)","level":2,"score":0.48666292428970337},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.355435311794281},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.2383384108543396},{"id":"https://openalex.org/C205649164","wikidata":"https://www.wikidata.org/wiki/Q1071","display_name":"Geography","level":0,"score":0.11526694893836975},{"id":"https://openalex.org/C153294291","wikidata":"https://www.wikidata.org/wiki/Q25261","display_name":"Meteorology","level":1,"score":0.09220138192176819}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.21769/bioprotoc.5398","doi":"https://doi.org/10.21769/bioprotoc.5398","is_oa":true,"landing_page_url":"https://doi.org/10.21769/bioprotoc.5398","pdf_url":null,"source":{"id":"https://openalex.org/S4210179406","display_name":"BIO-PROTOCOL","issn_l":"2331-8325","issn":["2331-8325"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320252","host_organization_name":"American Academy of Arts and Sciences","host_organization_lineage":["https://openalex.org/P4310320252"],"host_organization_lineage_names":["American Academy of Arts and Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BIO-PROTOCOL","raw_type":"journal-article"},{"id":"pmid:40741397","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/40741397","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Bio-protocol","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:12304474","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/12304474","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Bio Protoc","raw_type":"Text"},{"id":"pmh:oai:doaj.org/article:443ae426863142ba9c367a2b2e622569","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/443ae426863142ba9c367a2b2e622569","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Bio-Protocol, Vol 15, Iss 14 (2025)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.21769/bioprotoc.5398","doi":"https://doi.org/10.21769/bioprotoc.5398","is_oa":true,"landing_page_url":"https://doi.org/10.21769/bioprotoc.5398","pdf_url":null,"source":{"id":"https://openalex.org/S4210179406","display_name":"BIO-PROTOCOL","issn_l":"2331-8325","issn":["2331-8325"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","jufo-1","ki-jl-1","norway-1","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320252","host_organization_name":"American Academy of Arts and Sciences","host_organization_lineage":["https://openalex.org/P4310320252"],"host_organization_lineage_names":["American Academy of Arts and Sciences"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BIO-PROTOCOL","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.5299999713897705,"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":1,"referenced_works":["https://openalex.org/W1764704250"],"related_works":["https://openalex.org/W4238675884","https://openalex.org/W4238238172","https://openalex.org/W4236278520","https://openalex.org/W2349445879","https://openalex.org/W2315598954","https://openalex.org/W4386784614","https://openalex.org/W3022020934","https://openalex.org/W4389243014","https://openalex.org/W4200474776","https://openalex.org/W1655866052"],"abstract_inverted_index":{"at":[0,22,68,246],"100":[1,188],"mM":[2],"and":[3,10,20,39,88,107,138,152,162,176,216,228,241,269],"50%":[4],"methanol),":[5],"ZASP":[6,118,184,207,231,253],"depletes":[7],"harsh":[8],"detergents":[9],"impurities,":[11],"such":[12,125],"as":[13,126,192],"sodium":[14,127],"dodecyl":[15],"sulfate":[16],"(SDS),":[17],"Triton":[18],"X-100,":[19],"urea,":[21],"high":[23,163],"concentrations":[24],"in":[25,148,220],"solution":[26],"from":[27,58,93,236],"protein":[28,149,174,191,234],"solutions":[29],"prior":[30],"to":[31,109],"trypsin":[32,240],"digestion.":[33],"It":[34,46,144],"is":[35,194,208],"a":[36,102,209,221],"practical,":[37],"robust,":[38],"cost-effective":[40,217],"approach":[41],"for":[42,71,249],"proteomic":[43,218,256],"sample":[44,122,135,141,186],"preparation.":[45],"has":[47],"been":[48],"observed":[49],"that":[50,212],"90.2%":[51],"of":[52,66,77,96,104,115,168,183,190,202,258],"the":[53,181,200],"proteins":[54,87,106],"can":[55],"be":[56],"recovered":[57],"lysates":[59,237],"by":[60,244],"incubating":[61,245],"with":[62,112,172,187,238],"an":[63,83],"equal":[64],"volume":[65],"ZPB":[67],"room":[69,247],"temperature":[70,248,266],"10":[72,250],"min.":[73,251],"In":[74],"1":[75,94],"h":[76],"data-dependent":[78],"acquisition":[79],"(DDA)":[80],"analysis":[81,219,257],"on":[82],"Exploris":[84],"480,":[85],"4,037":[86],"25,626":[89],"peptides":[90,111],"were":[91],"quantified":[92],"\u03bcg":[95,114,189],"mouse":[97],"small":[98],"intestine":[99],"proteins,":[100],"reaching":[101],"peak":[103],"4,500":[105],"up":[108],"30,000":[110],"5":[113],"input.":[116],"Additionally,":[117],"outperformed":[119],"other":[120],"common":[121,222],"preparation":[123,136,142],"methods":[124],"deoxycholate":[128],"(SDC)-based":[129],"in-solution":[130],"digestion,":[131],"acetone":[132],"precipitation,":[133],"filter-aided":[134],"(FASP),":[137],"single-pot,":[139],"solid-phase-enhanced":[140],"(SP3).":[143],"demonstrated":[145],"superior":[146],"performance":[147],"(4,456":[150],"proteins)":[151],"peptide":[153],"identification":[154],"(29,871":[155],"peptides),":[156],"lower":[157,195],"missing":[158],"cleavage":[159],"rates":[160],"(16.3%),":[161],"reproducibility":[164],"(Pearson":[165],"correlation":[166],"coefficients":[167],"0.96":[169],"between":[170],"replicates)":[171],"similar":[173],"distributions":[175],"cellular":[177],"localization":[178],"patterns.":[179],"Significantly,":[180],"cost":[182],"per":[185],"input":[193],"than":[196],"30":[197],"RMB,":[198],"including":[199,262],"expense":[201],"trypsin.":[203],"Key":[204],"features":[205],"\u2022":[206,230,252],"user-friendly":[210],"method":[211],"enables":[213,232,254],"efficient,":[214],"sensitive,":[215],"biochemistry":[223],"lab":[224],"through":[225],"simple":[226],"incubation":[227],"centrifugation.":[229],"90%":[233],"recovery":[235],"various":[239],"LC-MS-incompatible":[242],"reagents":[243],"unbiased":[255],"diverse":[259],"biological":[260],"samples,":[261],"cells,":[263],"optimal":[264],"cutting":[265],"(OCT)-embedded":[267],"tissues,":[268],"formalin-fixed":[270],"paraffin-embedded":[271],"(FFPE)":[272],"tissues.":[273]},"counts_by_year":[],"updated_date":"2026-10-14T16:39:01.820549","created_date":"2025-07-14T00:00:00"}