{"id":"https://openalex.org/W4412198540","doi":"https://doi.org/10.1021/acssuschemeng.5c02872","title":"Efficient Biosynthesis of N-Acetyllactosamine by a Metabolically Engineered Escherichia coli BL21(DE3) Strain","display_name":"Efficient Biosynthesis of N-Acetyllactosamine by a Metabolically Engineered Escherichia coli BL21(DE3) Strain","publication_year":2025,"publication_date":"2025-07-11","ids":{"openalex":"https://openalex.org/W4412198540","doi":"https://doi.org/10.1021/acssuschemeng.5c02872"},"language":"en","primary_location":{"id":"doi:10.1021/acssuschemeng.5c02872","doi":"https://doi.org/10.1021/acssuschemeng.5c02872","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acssuschemeng.5c02872","pdf_url":null,"source":{"id":"https://openalex.org/S2502047864","display_name":"ACS Sustainable Chemistry & Engineering","issn_l":"2168-0485","issn":["2168-0485"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","ki-jl-1","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Sustainable Chemistry & Engineering","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5012786000","display_name":"Guocong Luo","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I111599522","display_name":"Jiangnan University","ror":"https://ror.org/04mkzax54","country_code":"CN","type":"education","lineage":["https://openalex.org/I111599522"]},{"id":"https://openalex.org/I4210152154","display_name":"State Key Laboratory of Food Science and Technology","ror":"https://ror.org/04zgj7914","country_code":"CN","type":"facility","lineage":["https://openalex.org/I111599522","https://openalex.org/I141649914","https://openalex.org/I4210152154"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Guocong Luo","raw_affiliation_strings":["Jiangnan University","State Key Laboratory of Food Science and Resources, School of Food Science and Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Jiangnan University","institution_ids":["https://openalex.org/I111599522"]},{"raw_affiliation_string":"State Key Laboratory of Food Science and Resources, School of Food Science and Technology","institution_ids":["https://openalex.org/I4210152154"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110552639","display_name":"Zhiyi Zhang","orcid":"https://orcid.org/0000-0003-3951-3834","observed_orcids":["https://orcid.org/0000-0003-3951-3834"]},"institutions":[{"id":"https://openalex.org/I111599522","display_name":"Jiangnan University","ror":"https://ror.org/04mkzax54","country_code":"CN","type":"education","lineage":["https://openalex.org/I111599522"]},{"id":"https://openalex.org/I4210152154","display_name":"State Key Laboratory of Food Science and Technology","ror":"https://ror.org/04zgj7914","country_code":"CN","type":"facility","lineage":["https://openalex.org/I111599522","https://openalex.org/I141649914","https://openalex.org/I4210152154"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Zhiyi Zhang","raw_affiliation_strings":["Jiangnan University","State Key Laboratory of Food Science and Resources, School of Food Science and Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Jiangnan University","institution_ids":["https://openalex.org/I111599522"]},{"raw_affiliation_string":"State Key Laboratory of Food Science and Resources, School of Food Science and Technology","institution_ids":["https://openalex.org/I4210152154"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100398474","display_name":"Wenbo Zhang","orcid":"https://orcid.org/0000-0003-4682-354X","observed_orcids":["https://orcid.org/0000-0003-4682-354X"]},"institutions":[{"id":"https://openalex.org/I111599522","display_name":"Jiangnan University","ror":"https://ror.org/04mkzax54","country_code":"CN","type":"education","lineage":["https://openalex.org/I111599522"]},{"id":"https://openalex.org/I4210152154","display_name":"State Key Laboratory of Food Science and Technology","ror":"https://ror.org/04zgj7914","country_code":"CN","type":"facility","lineage":["https://openalex.org/I111599522","https://openalex.org/I141649914","https://openalex.org/I4210152154"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wenbo Zhang","raw_affiliation_strings":["Jiangnan University","State Key Laboratory of Food Science and Resources, School of Food Science and Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Jiangnan University","institution_ids":["https://openalex.org/I111599522"]},{"raw_affiliation_string":"State Key Laboratory of Food Science and Resources, School of Food Science and Technology","institution_ids":["https://openalex.org/I4210152154"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5114755597","display_name":"Qianyi Qian","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I111599522","display_name":"Jiangnan University","ror":"https://ror.org/04mkzax54","country_code":"CN","type":"education","lineage":["https://openalex.org/I111599522"]},{"id":"https://openalex.org/I4210152154","display_name":"State Key Laboratory of Food Science and Technology","ror":"https://ror.org/04zgj7914","country_code":"CN","type":"facility","lineage":["https://openalex.org/I111599522","https://openalex.org/I141649914","https://openalex.org/I4210152154"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qianyi Qian","raw_affiliation_strings":["Jiangnan University","State Key Laboratory of Food Science and Resources, School of Food Science and Technology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Jiangnan University","institution_ids":["https://openalex.org/I111599522"]},{"raw_affiliation_string":"State Key Laboratory of Food Science and Resources, School of Food Science and Technology","institution_ids":["https://openalex.org/I4210152154"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002058361","display_name":"Yingying Zhu","orcid":"https://orcid.org/0009-0003-3784-846X","observed_orcids":["https://orcid.org/0009-0003-3784-846X"]},"institutions":[{"id":"https://openalex.org/I111599522","display_name":"Jiangnan University","ror":"https://ror.org/04mkzax54","country_code":"CN","type":"education","lineage":["https://openalex.org/I111599522"]},{"id":"https://openalex.org/I4210152154","display_name":"State Key Laboratory of Food Science and Technology","ror":"https://ror.org/04zgj7914","country_code":"CN","type":"facility","lineage":["https://openalex.org/I111599522","https://openalex.org/I141649914","https://openalex.org/I4210152154"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yingying Zhu","raw_affiliation_strings":["Jiangnan University","State Key Laboratory of Food Science and Resources, School of Food Science and Technology"],"raw_orcid":"https://orcid.org/0009-0003-3784-846X","affiliations":[{"raw_affiliation_string":"Jiangnan University","institution_ids":["https://openalex.org/I111599522"]},{"raw_affiliation_string":"State Key Laboratory of Food Science and Resources, School of Food Science and Technology","institution_ids":["https://openalex.org/I4210152154"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100318943","display_name":"Wenli Zhang","orcid":"https://orcid.org/0000-0002-7861-0170","observed_orcids":["https://orcid.org/0000-0002-7861-0170"]},"institutions":[{"id":"https://openalex.org/I111599522","display_name":"Jiangnan University","ror":"https://ror.org/04mkzax54","country_code":"CN","type":"education","lineage":["https://openalex.org/I111599522"]},{"id":"https://openalex.org/I4210152154","display_name":"State Key Laboratory of Food Science and Technology","ror":"https://ror.org/04zgj7914","country_code":"CN","type":"facility","lineage":["https://openalex.org/I111599522","https://openalex.org/I141649914","https://openalex.org/I4210152154"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wenli Zhang","raw_affiliation_strings":["Jiangnan University","State Key Laboratory of Food Science and Resources, School of Food Science and Technology"],"raw_orcid":"https://orcid.org/0000-0002-7861-0170","affiliations":[{"raw_affiliation_string":"Jiangnan University","institution_ids":["https://openalex.org/I111599522"]},{"raw_affiliation_string":"State Key Laboratory of Food Science and Resources, School of Food Science and Technology","institution_ids":["https://openalex.org/I4210152154"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5085774449","display_name":"Wanmeng Mu","orcid":"https://orcid.org/0000-0001-6597-527X","observed_orcids":["https://orcid.org/0000-0001-6597-527X"]},"institutions":[{"id":"https://openalex.org/I111599522","display_name":"Jiangnan University","ror":"https://ror.org/04mkzax54","country_code":"CN","type":"education","lineage":["https://openalex.org/I111599522"]},{"id":"https://openalex.org/I4210152154","display_name":"State Key Laboratory of Food Science and Technology","ror":"https://ror.org/04zgj7914","country_code":"CN","type":"facility","lineage":["https://openalex.org/I111599522","https://openalex.org/I141649914","https://openalex.org/I4210152154"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Wanmeng Mu","raw_affiliation_strings":["Jiangnan University","State Key Laboratory of Food Science and Resources, School of Food Science and Technology"],"raw_orcid":"https://orcid.org/0000-0001-6597-527X","affiliations":[{"raw_affiliation_string":"Jiangnan University","institution_ids":["https://openalex.org/I111599522"]},{"raw_affiliation_string":"State Key Laboratory of Food Science and Resources, School of Food Science and Technology","institution_ids":["https://openalex.org/I4210152154"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5085774449"],"corresponding_institution_ids":["https://openalex.org/I111599522","https://openalex.org/I4210152154"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.8831,"has_fulltext":false,"cited_by_count":4,"citation_normalized_percentile":{"value":0.65664652,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"13","issue":"28","first_page":"10898","last_page":"10907"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.003700000001117587,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12489","display_name":"Microbial Metabolites in Food Biotechnology","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/2916","display_name":"Nutrition and Dietetics"},"field":{"id":"https://openalex.org/fields/29","display_name":"Nursing"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/n-acetyllactosamine","display_name":"N-acetyllactosamine","score":0.9570000171661377},{"id":"https://openalex.org/keywords/human-milk-oligosaccharides","display_name":"human milk oligosaccharides","score":0.9340000152587891},{"id":"https://openalex.org/keywords/escherichia-coli-bl21-de3","display_name":"Escherichia coli BL21(DE3)","score":0.8600000143051147},{"id":"https://openalex.org/keywords/metabolic-engineering","display_name":"metabolic engineering","score":0.7149999737739563},{"id":"https://openalex.org/keywords/beta-1-4-galactosyltransferase","display_name":"beta-1,4-galactosyltransferase","score":0.9679999947547913},{"id":"https://openalex.org/keywords/helicobacter-pylori","display_name":"Helicobacter pylori","score":0.9950000047683716},{"id":"https://openalex.org/keywords/glycosyltransferases","display_name":"glycosyltransferases","score":0.7310000061988831},{"id":"https://openalex.org/keywords/glycerol-carbon-source","display_name":"glycerol carbon source","score":0.9440000057220459},{"id":"https://openalex.org/keywords/fed-batch-fermentation","display_name":"fed-batch fermentation","score":0.9079999923706055}],"concepts":[{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.8688009977340698},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.7818769216537476},{"id":"https://openalex.org/C2778022156","wikidata":"https://www.wikidata.org/wiki/Q576145","display_name":"Strain (injury)","level":2,"score":0.7412970662117004},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5680344104766846},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.45532065629959106},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.32219600677490234},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.29331690073013306},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.14471358060836792},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.14088353514671326},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.05227115750312805}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1021/acssuschemeng.5c02872","doi":"https://doi.org/10.1021/acssuschemeng.5c02872","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acssuschemeng.5c02872","pdf_url":null,"source":{"id":"https://openalex.org/S2502047864","display_name":"ACS Sustainable Chemistry & Engineering","issn_l":"2168-0485","issn":["2168-0485"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","ki-jl-1","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Sustainable Chemistry & Engineering","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2440208734","display_name":null,"funder_award_id":"2022YFD2104900","funder_id":"https://openalex.org/F4320335777","funder_display_name":"National Key Research and Development Program of China"},{"id":"https://openalex.org/G2732206963","display_name":null,"funder_award_id":"JUSRP622008","funder_id":"https://openalex.org/F4320335787","funder_display_name":"Fundamental Research Funds for the Central Universities"}],"funders":[{"id":"https://openalex.org/F4320335777","display_name":"National Key Research and Development Program of China","ror":null},{"id":"https://openalex.org/F4320335787","display_name":"Fundamental Research Funds for the Central Universities","ror":null}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":40,"referenced_works":["https://openalex.org/W1971416176","https://openalex.org/W1990272534","https://openalex.org/W1995979783","https://openalex.org/W1997396836","https://openalex.org/W1998487735","https://openalex.org/W2016603560","https://openalex.org/W2024392981","https://openalex.org/W2054022646","https://openalex.org/W2074002745","https://openalex.org/W2085648335","https://openalex.org/W2132602091","https://openalex.org/W2135541178","https://openalex.org/W2519185218","https://openalex.org/W2694440931","https://openalex.org/W2896502782","https://openalex.org/W2990008714","https://openalex.org/W3008768822","https://openalex.org/W3087773123","https://openalex.org/W3093721850","https://openalex.org/W3137153611","https://openalex.org/W3160717427","https://openalex.org/W3161928102","https://openalex.org/W3185548808","https://openalex.org/W3187990360","https://openalex.org/W3197441994","https://openalex.org/W4205965654","https://openalex.org/W4210707057","https://openalex.org/W4224608741","https://openalex.org/W4284882685","https://openalex.org/W4292314403","https://openalex.org/W4310948592","https://openalex.org/W4323294116","https://openalex.org/W4383302750","https://openalex.org/W4384818280","https://openalex.org/W4387728283","https://openalex.org/W4391258264","https://openalex.org/W4402405036","https://openalex.org/W4404630839","https://openalex.org/W4404960299","https://openalex.org/W4405740189"],"related_works":["https://openalex.org/W7165367982","https://openalex.org/W4411217667","https://openalex.org/W7154914203","https://openalex.org/W4408143679","https://openalex.org/W7125836113","https://openalex.org/W3137153611","https://openalex.org/W4292314403","https://openalex.org/W7213933312","https://openalex.org/W3204569115","https://openalex.org/W4417214447"],"abstract_inverted_index":{"The":[0,86,146],"biological":[1,30],"synthesis":[2,44,143],"of":[3,11,19,32,45,68,88,108,123,154,176],"human":[4],"milk":[5,47],"oligosaccharides":[6,48],"(HMOs)":[7],"through":[8],"the":[9,29,43,55,66,93,103,109,117,126,174],"use":[10],"metabolically":[12],"engineered":[13,148,177],"strains":[14],"represents":[15],"a":[16,39,79,136,164,188],"significant":[17],"area":[18],"interest.":[20],"However,":[21],"comparatively":[22],"less":[23],"attention":[24],"has":[25],"been":[26],"directed":[27],"to":[28,115,134],"production":[31,152,182],"N":[33,94],"-acetyllactosamine":[34],"(LacNAc).":[35],"LacNAc":[36,59,84,142,151,181],"serves":[37],"as":[38,78],"key":[40],"intermediate":[41],"in":[42,60,125,157,163],"sialylated":[46],"(SMOs).":[49],"In":[50],"this":[51],"study,":[52],"we":[53],"constructed":[54],"metabolic":[56,70,184],"pathway":[57,97,190],"for":[58,82,141,180,191],"Escherichia":[61],"coli":[62,179],"BL21(DE3).":[63],"To":[64],"facilitate":[65],"design":[67],"artificial":[69],"pathways,":[71],"\u03b2-1,4-galactosyltransferase":[72],"from":[73],"Helicobacter":[74],"pylori":[75],"was":[76,98,113,132],"identified":[77],"critical":[80],"glycosyltransferase":[81],"enhancing":[83],"production.":[85],"expression":[87,118],"essential":[89],"gene":[90,110],"clusters":[91],"within":[92],"-acetylglucosamine":[95],"(GlcNAc)":[96],"augmented":[99],"and":[100,120,128,160,186],"integrated":[101],"into":[102],"chromosome.":[104],"Subsequently,":[105],"combinatorial":[106],"optimization":[107],"copy":[111],"number":[112],"conducted":[114],"modulate":[116],"strength":[119],"translation":[121],"rates":[122],"enzymes":[124],"GlcNAc":[127],"UDP-Gal":[129],"pathways.":[130],"Glycerol":[131],"determined":[133],"be":[135],"more":[137],"effective":[138],"carbon":[139],"source":[140],"than":[144],"glucose.":[145],"final":[147],"strain":[149],"achieved":[150],"levels":[153],"6.32":[155],"g/L":[156,162],"shake-flask":[158],"cultures":[159],"38.26":[161],"5":[165],"L":[166],"bioreactor":[167],"using":[168],"fed-batch":[169],"fermentation.":[170],"This":[171],"work":[172],"demonstrates":[173],"potential":[175],"E.":[178],"via":[183],"engineering":[185],"establishes":[187],"foundational":[189],"SMO":[192],"synthesis.":[193]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":1}],"updated_date":"2026-09-21T07:43:02.447757","created_date":"2025-10-10T00:00:00"}