{"id":"https://openalex.org/W4411470995","doi":"https://doi.org/10.7554/elife.102305.3","title":"Cardiac Fibroblasts regulate myocardium and coronary vasculature development via the collagen signaling pathway","display_name":"Cardiac Fibroblasts regulate myocardium and coronary vasculature development via the collagen signaling pathway","publication_year":2025,"publication_date":"2025-06-20","ids":{"openalex":"https://openalex.org/W4411470995","doi":"https://doi.org/10.7554/elife.102305.3"},"language":"en","primary_location":{"id":"doi:10.7554/elife.102305.3","doi":"https://doi.org/10.7554/elife.102305.3","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.102305.3","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"eLife","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.7554/elife.102305.3","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5016050496","display_name":"Yiting Deng","orcid":"https://orcid.org/0000-0002-4316-1440","observed_orcids":["https://orcid.org/0000-0002-4316-1440"]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yiting Deng","raw_affiliation_strings":["Department of Cell Biology, University of Pittsburgh School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, University of Pittsburgh School of Medicine","institution_ids":["https://openalex.org/I170201317"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005644734","display_name":"Yuanhang He","orcid":"https://orcid.org/0000-0002-6439-4315","observed_orcids":["https://orcid.org/0000-0002-6439-4315"]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]},{"id":"https://openalex.org/I99065089","display_name":"Tsinghua University","ror":"https://ror.org/03cve4549","country_code":"CN","type":"education","lineage":["https://openalex.org/I99065089"]}],"countries":["CN","US"],"is_corresponding":false,"raw_author_name":"Yuanhang He","raw_affiliation_strings":["Department of Cell Biology, University of Pittsburgh School of Medicine","Tsinghua University, Tsinghua medicine, School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, University of Pittsburgh School of Medicine","institution_ids":["https://openalex.org/I170201317"]},{"raw_affiliation_string":"Tsinghua University, Tsinghua medicine, School of Medicine","institution_ids":["https://openalex.org/I99065089"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102000587","display_name":"Juan Xu","orcid":"https://orcid.org/0000-0002-6486-0128","observed_orcids":["https://orcid.org/0000-0002-6486-0128"]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Juan Xu","raw_affiliation_strings":["Department of Cell Biology, University of Pittsburgh School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, University of Pittsburgh School of Medicine","institution_ids":["https://openalex.org/I170201317"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074698598","display_name":"Haoting He","orcid":"https://orcid.org/0009-0007-2031-353X","observed_orcids":["https://orcid.org/0009-0007-2031-353X"]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Haoting He","raw_affiliation_strings":["Department of Cell Biology, University of Pittsburgh School of Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Cell Biology, University of Pittsburgh School of Medicine","institution_ids":["https://openalex.org/I170201317"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5115855477","display_name":"Manling Zhang","orcid":"https://orcid.org/0000-0002-5447-2615","observed_orcids":["https://orcid.org/0000-0002-5447-2615"]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Manling Zhang","raw_affiliation_strings":["Vascular Medicine Institute, Department of Medicine, University of Pittsburgh"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Vascular Medicine Institute, Department of Medicine, University of Pittsburgh","institution_ids":["https://openalex.org/I170201317"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100406312","display_name":"Guang Li","orcid":"https://orcid.org/0000-0002-8546-2364","observed_orcids":["https://orcid.org/0000-0002-8546-2364"]},"institutions":[{"id":"https://openalex.org/I170201317","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305","country_code":"US","type":"education","lineage":["https://openalex.org/I170201317"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Guang Li","raw_affiliation_strings":["Department of Cell Biology, University of Pittsburgh School of Medicine"],"raw_orcid":"https://orcid.org/0000-0002-8546-2364","affiliations":[{"raw_affiliation_string":"Department of Cell Biology, University of Pittsburgh School of Medicine","institution_ids":["https://openalex.org/I170201317"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3000,"currency":"USD","value_usd":2500},"apc_paid":{"value":3000,"currency":"USD","value_usd":2500},"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11677","display_name":"Congenital heart defects research","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11677","display_name":"Congenital heart defects research","score":0.9951000213623047,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11962","display_name":"Cardiac Fibrosis and Remodeling","score":0.004100000020116568,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10217","display_name":"Cardiac electrophysiology and arrhythmias","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cardiac-fibroblasts","display_name":"cardiac fibroblasts","score":1.0},{"id":"https://openalex.org/keywords/myocardial-development","display_name":"myocardial development","score":0.8270000219345093},{"id":"https://openalex.org/keywords/coronary-vasculature-development","display_name":"coronary vasculature development","score":0.9810000061988831},{"id":"https://openalex.org/keywords/collagen-signaling","display_name":"collagen signaling","score":0.8960000276565552},{"id":"https://openalex.org/keywords/cardiomyocytes","display_name":"cardiomyocytes","score":0.7699999809265137},{"id":"https://openalex.org/keywords/vascular-endothelial-cells","display_name":"vascular endothelial cells","score":0.9700000286102295}],"concepts":[{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.5157292485237122},{"id":"https://openalex.org/C2780381497","wikidata":"https://www.wikidata.org/wiki/Q463418","display_name":"Fibroblast","level":3,"score":0.5013325214385986},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.49242648482322693},{"id":"https://openalex.org/C2777837822","wikidata":"https://www.wikidata.org/wiki/Q14819288","display_name":"Embryonic heart","level":4,"score":0.4855179786682129},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4835819602012634},{"id":"https://openalex.org/C62425271","wikidata":"https://www.wikidata.org/wiki/Q14819288","display_name":"Heart development","level":4,"score":0.42128512263298035},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.4198974370956421},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.30890077352523804},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.22487440705299377},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.14339423179626465}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.7554/elife.102305.3","doi":"https://doi.org/10.7554/elife.102305.3","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.102305.3","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"eLife","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.7554/elife.102305.3","doi":"https://doi.org/10.7554/elife.102305.3","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.102305.3","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"eLife","raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.8371999859809875,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320310174","display_name":"University of Pittsburgh","ror":"https://ror.org/01an3r305"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":43,"referenced_works":["https://openalex.org/W1891191024","https://openalex.org/W1964179882","https://openalex.org/W1979711120","https://openalex.org/W2005893713","https://openalex.org/W2016460143","https://openalex.org/W2040330595","https://openalex.org/W2045295282","https://openalex.org/W2052811457","https://openalex.org/W2079641406","https://openalex.org/W2084252683","https://openalex.org/W2104010513","https://openalex.org/W2118221757","https://openalex.org/W2125543831","https://openalex.org/W2183587606","https://openalex.org/W2257383956","https://openalex.org/W2345376732","https://openalex.org/W2482213414","https://openalex.org/W2530928249","https://openalex.org/W2608644283","https://openalex.org/W2616922646","https://openalex.org/W2783441930","https://openalex.org/W2800406470","https://openalex.org/W2911685613","https://openalex.org/W2951068818","https://openalex.org/W2977756124","https://openalex.org/W2979684492","https://openalex.org/W2984472267","https://openalex.org/W2992151131","https://openalex.org/W2996171578","https://openalex.org/W2997366069","https://openalex.org/W3007935255","https://openalex.org/W3029730275","https://openalex.org/W3038763010","https://openalex.org/W3073734492","https://openalex.org/W3126754513","https://openalex.org/W3132661792","https://openalex.org/W3181267383","https://openalex.org/W4280527818","https://openalex.org/W4282932514","https://openalex.org/W4296805857","https://openalex.org/W4307775633","https://openalex.org/W4312201129","https://openalex.org/W4378212544"],"related_works":["https://openalex.org/W4411870789","https://openalex.org/W2942264028","https://openalex.org/W2598538543","https://openalex.org/W2022409702","https://openalex.org/W2516184834","https://openalex.org/W4296805857","https://openalex.org/W4401809410","https://openalex.org/W2013142219","https://openalex.org/W1997441326","https://openalex.org/W2022238204"],"abstract_inverted_index":{"Abstract":[0],"The":[1],"fibroblast":[2,203],"(FB),":[3],"cardiomyocyte":[4],"(CM),":[5],"and":[6,49,70,75,106,136,139,153,161,178,201,219,248,251],"vascular":[7],"endothelial":[8],"cell":[9,15],"(Vas_EC)":[10],"are":[11,25],"the":[12,18,37,44,50,64,85,97,122,130,150,175,182,187,192,206,241,257],"three":[13],"major":[14,183],"types":[16],"in":[17,157,174,191,217,245,260],"heart,":[19],"yet":[20],"their":[21,158],"relationships":[22],"during":[23],"development":[24,138,250],"largely":[26],"unexplored.":[27],"To":[28],"address":[29],"this":[30,261],"gap,":[31],"we":[32,93,110,147,168,197],"employed":[33],"RNA":[34],"staining":[35],"of":[36,67,87,132,172,243],"FB":[38],"marker":[39,46,52],"gene":[40,47,53],"Col1a1":[41],"together":[42],"with":[43,80],"CM":[45,105],"Actn2":[48],"Vas_EC":[51,107,220],"Cdh5":[54],"at":[55,124,205],"different":[56,125],"stages.":[57,126],"This":[58],"approach":[59],"enabled":[60],"us":[61],"to":[62,120,141,164],"discern":[63],"anatomical":[65],"pattern":[66],"cardiac":[68],"FBs":[69,103,123,133],"identify":[71],"approximately":[72],"one":[73],"EC":[74],"four":[76],"CMs":[77,160],"directly":[78],"interacting":[79],"each":[81],"FB.":[82],"Molecularly,":[83],"through":[84],"analysis":[86],"single-cell":[88],"mRNA":[89],"sequencing":[90],"(scRNA-seq)":[91],"data,":[92],"unveiled":[94],"collagen":[95,173,180,218,228,258],"as":[96,181],"top":[98],"signaling":[99,184],"molecule":[100],"derived":[101],"from":[102],"influencing":[104],"development.":[108],"Subsequently,":[109],"used":[111],"a":[112,170,215],"Pdgfra-CreER":[113],"controlled":[114],"diphtheria":[115],"toxin":[116],"A":[117],"(DTA)":[118],"system":[119],"ablate":[121],"We":[127,209],"found":[128,210],"that":[129,211],"ablation":[131,204,213,224],"disrupted":[134],"myocardium":[135,247],"vasculature":[137,249],"led":[140],"embryonic":[142],"heart":[143,232],"defects.":[144],"Using":[145],"scRNA-seq,":[146],"further":[148],"profiled":[149],"ablated":[151,176,193],"hearts":[152,177],"identified":[154,169,240],"molecular":[155],"defects":[156],"ventricular":[159,194],"Vas_ECs":[162],"compared":[163],"control":[165],"hearts.":[166],"Moreover,":[167],"reduction":[171,216],"predicted":[179],"pathway":[185,259],"regulating":[186,246],"differentially":[188],"expressed":[189],"genes":[190],"CMs.":[195],"Finally,":[196],"performed":[198],"both":[199],"short-term":[200,212],"long-term":[202,223],"neonatal":[207],"stage.":[208],"caused":[214],"density,":[221],"while":[222],"may":[225],"induce":[226],"compensatory":[227],"expression":[229],"without":[230],"causing":[231],"function":[233,242],"reduction.":[234],"In":[235],"summary,":[236],"our":[237],"study":[238],"has":[239],"fibroblasts":[244],"implicated":[252],"an":[253],"important":[254],"role":[255],"for":[256],"process.":[262]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2025-10-10T00:00:00"}