{"id":"https://openalex.org/W4411079192","doi":"https://doi.org/10.1016/j.mex.2025.103406","title":"Viable E. coli O157:H7 detection based on F\u00f6ster resonance energy transfer (FRET) system using FITC and TRITC conjugates as molecular probes","display_name":"Viable E. coli O157:H7 detection based on F\u00f6ster resonance energy transfer (FRET) system using FITC and TRITC conjugates as molecular probes","publication_year":2025,"publication_date":"2025-06-01","ids":{"openalex":"https://openalex.org/W4411079192","doi":"https://doi.org/10.1016/j.mex.2025.103406","pmid":"https://pubmed.ncbi.nlm.nih.gov/40584166","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/12205779"},"language":"en","primary_location":{"id":"doi:10.1016/j.mex.2025.103406","doi":"https://doi.org/10.1016/j.mex.2025.103406","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.mex.2025.103406","pdf_url":null,"source":{"id":"https://openalex.org/S2898269294","display_name":"MethodsX","issn_l":"2215-0161","issn":["2215-0161"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-d","norway-1","poland-70"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"MethodsX","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1016/j.mex.2025.103406","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5048941044","display_name":"Nasruddeen Al\u2010Awwal","orcid":"https://orcid.org/0000-0001-8172-4913","observed_orcids":["https://orcid.org/0000-0001-8172-4913"]},"institutions":[{"id":"https://openalex.org/I151651118","display_name":"Lincoln University - Missouri","ror":"https://ror.org/05hn3aw08","country_code":"US","type":"education","lineage":["https://openalex.org/I151651118"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Nasruddeen Al-Awwal","raw_affiliation_strings":["Cooperative Research and Extension, College of Agriculture, Environmental and Human Sciences, Lincoln University Missouri 65101, USA"],"raw_orcid":"https://orcid.org/0000-0001-8172-4913","affiliations":[{"raw_affiliation_string":"Cooperative Research and Extension, College of Agriculture, Environmental and Human Sciences, Lincoln University Missouri 65101, USA","institution_ids":["https://openalex.org/I151651118"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058590812","display_name":"Samira Mahdi","orcid":"https://orcid.org/0000-0002-8354-1050","observed_orcids":["https://orcid.org/0000-0002-8354-1050"]},"institutions":[{"id":"https://openalex.org/I151651118","display_name":"Lincoln University - Missouri","ror":"https://ror.org/05hn3aw08","country_code":"US","type":"education","lineage":["https://openalex.org/I151651118"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Samira Mahdi","raw_affiliation_strings":["Cooperative Research and Extension, College of Agriculture, Environmental and Human Sciences, Lincoln University Missouri 65101, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Cooperative Research and Extension, College of Agriculture, Environmental and Human Sciences, Lincoln University Missouri 65101, USA","institution_ids":["https://openalex.org/I151651118"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064279608","display_name":"Majed El\u2010Dweik","orcid":"https://orcid.org/0000-0001-7701-0686","observed_orcids":["https://orcid.org/0000-0001-7701-0686"]},"institutions":[{"id":"https://openalex.org/I176692203","display_name":"Alabama Agricultural and Mechanical University","ror":"https://ror.org/05hz8m414","country_code":"US","type":"education","lineage":["https://openalex.org/I176692203"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Majed El-Dweik","raw_affiliation_strings":["Office of the Vice President Research and Economic Development Alabama A&M University, Huntsville AL 35811, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Office of the Vice President Research and Economic Development Alabama A&M University, Huntsville AL 35811, USA","institution_ids":["https://openalex.org/I176692203"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073811765","display_name":"Stephen H. Anderson","orcid":"https://orcid.org/0000-0002-5664-6121","observed_orcids":["https://orcid.org/0000-0002-5664-6121"]},"institutions":[{"id":"https://openalex.org/I76835614","display_name":"University of Missouri","ror":"https://ror.org/02ymw8z06","country_code":"US","type":"education","lineage":["https://openalex.org/I76835614"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Stephen H. Anderson","raw_affiliation_strings":["School of Natural Resources, University of Missouri Columbia, 321 Anheuser-Busch Natural Resources Building 65211, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Natural Resources, University of Missouri Columbia, 321 Anheuser-Busch Natural Resources Building 65211, USA","institution_ids":["https://openalex.org/I76835614"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5021134038","display_name":"Tumen Wuliji","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I151651118","display_name":"Lincoln University - Missouri","ror":"https://ror.org/05hn3aw08","country_code":"US","type":"education","lineage":["https://openalex.org/I151651118"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tumen Wuliji","raw_affiliation_strings":["Small Animal Research Facility, Lincoln University Missouri 65101, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Small Animal Research Facility, Lincoln University Missouri 65101, USA","institution_ids":["https://openalex.org/I151651118"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5048941044"],"corresponding_institution_ids":["https://openalex.org/I151651118"],"apc_list":{"value":1260,"currency":"USD","value_usd":1260},"apc_paid":{"value":1260,"currency":"USD","value_usd":1260},"fwci":0.2029,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":{"value":0.33064219,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":91,"max":95},"biblio":{"volume":"14","issue":null,"first_page":"103406","last_page":"103406"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.8726000189781189,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.8726000189781189,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10943","display_name":"Escherichia coli research studies","score":0.09189999848604202,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.0071000000461936,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/escherichia-coli-o157-h7","display_name":"Escherichia coli O157:H7","score":0.9919999837875366},{"id":"https://openalex.org/keywords/foster-resonance-energy-transfer","display_name":"F\u00f6ster resonance energy transfer","score":0.9359999895095825},{"id":"https://openalex.org/keywords/fret-based-biosensors","display_name":"FRET biosensor","score":0.796999990940094},{"id":"https://openalex.org/keywords/sandwich-immunoassay","display_name":"sandwich immunoassay","score":0.8140000104904175},{"id":"https://openalex.org/keywords/fitc-labeled-antibodies","display_name":"FITC-labeled antibodies","score":0.7820000052452087},{"id":"https://openalex.org/keywords/foodborne-pathogen-detection","display_name":"foodborne pathogen detection","score":0.824999988079071},{"id":"https://openalex.org/keywords/fluorescence-imaging","display_name":"fluorescence imaging","score":0.527999997138977},{"id":"https://openalex.org/keywords/pre-enrichment","display_name":"pre-enrichment","score":0.765999972820282}],"concepts":[{"id":"https://openalex.org/C96305047","wikidata":"https://www.wikidata.org/wiki/Q367473","display_name":"F\u00f6rster resonance energy transfer","level":3,"score":0.9571274518966675},{"id":"https://openalex.org/C197336794","wikidata":"https://www.wikidata.org/wiki/Q5161150","display_name":"Conjugate","level":2,"score":0.6778866648674011},{"id":"https://openalex.org/C106447425","wikidata":"https://www.wikidata.org/wiki/Q11379","display_name":"Energy transfer","level":2,"score":0.5569230318069458},{"id":"https://openalex.org/C139210041","wikidata":"https://www.wikidata.org/wiki/Q2145840","display_name":"Resonance (particle physics)","level":2,"score":0.4385581314563751},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4154165983200073},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.27511608600616455},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.19377848505973816},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.14069408178329468},{"id":"https://openalex.org/C41999313","wikidata":"https://www.wikidata.org/wiki/Q489328","display_name":"Molecular physics","level":1,"score":0.09422802925109863},{"id":"https://openalex.org/C184779094","wikidata":"https://www.wikidata.org/wiki/Q26383","display_name":"Atomic physics","level":1,"score":0.08086782693862915},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.0},{"id":"https://openalex.org/C134306372","wikidata":"https://www.wikidata.org/wiki/Q7754","display_name":"Mathematical analysis","level":1,"score":0.0}],"mesh":[],"locations_count":5,"locations":[{"id":"doi:10.1016/j.mex.2025.103406","doi":"https://doi.org/10.1016/j.mex.2025.103406","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.mex.2025.103406","pdf_url":null,"source":{"id":"https://openalex.org/S2898269294","display_name":"MethodsX","issn_l":"2215-0161","issn":["2215-0161"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-d","norway-1","poland-70"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"MethodsX","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:12205779","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12205779/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12205779/pdf/","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"MethodsX","raw_type":"Text"},{"id":"pmh:oai:europepmc.org:11012981","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/12205779","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmid:40584166","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/40584166","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"MethodsX","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:777b5386e2f345118e51d2246d6a2402","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/777b5386e2f345118e51d2246d6a2402","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"MethodsX, Vol 14, Iss , Pp 103406- (2025)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1016/j.mex.2025.103406","doi":"https://doi.org/10.1016/j.mex.2025.103406","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.mex.2025.103406","pdf_url":null,"source":{"id":"https://openalex.org/S2898269294","display_name":"MethodsX","issn_l":"2215-0161","issn":["2215-0161"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-d","norway-1","poland-70"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"MethodsX","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306114","display_name":"U.S. Department of Agriculture","ror":"https://ror.org/01na82s61"},{"id":"https://openalex.org/F4320309450","display_name":"Lincoln University","ror":"https://ror.org/0521rfb23"},{"id":"https://openalex.org/F4320332299","display_name":"National Institute of Food and Agriculture","ror":"https://ror.org/05qx3fv49"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4411079192.pdf","grobid_xml":"https://content.openalex.org/works/W4411079192.grobid-xml"},"referenced_works_count":26,"referenced_works":["https://openalex.org/W1490295882","https://openalex.org/W1607296247","https://openalex.org/W1805028853","https://openalex.org/W1975420686","https://openalex.org/W1975980627","https://openalex.org/W1995800834","https://openalex.org/W1997012332","https://openalex.org/W2019851569","https://openalex.org/W2126002517","https://openalex.org/W2131995085","https://openalex.org/W2136881872","https://openalex.org/W2167373328","https://openalex.org/W2382739494","https://openalex.org/W2564279034","https://openalex.org/W2593469957","https://openalex.org/W2620345815","https://openalex.org/W2753290925","https://openalex.org/W2767366555","https://openalex.org/W2778052905","https://openalex.org/W2800420367","https://openalex.org/W2898574255","https://openalex.org/W3023625558","https://openalex.org/W3025092886","https://openalex.org/W3115099860","https://openalex.org/W3115450492","https://openalex.org/W4205771092"],"related_works":["https://openalex.org/W4401550693","https://openalex.org/W4392095811","https://openalex.org/W2767535175","https://openalex.org/W2344724268","https://openalex.org/W4312142586","https://openalex.org/W2124894637","https://openalex.org/W4321459562","https://openalex.org/W4388219224","https://openalex.org/W2399369605","https://openalex.org/W3147883653"],"abstract_inverted_index":{"Improved":[0],"rapid":[1],"and":[2,24,28,67,126,137],"sensitive":[3,29,114],"biosensors":[4],"for":[5,31],"detecting":[6,32],"foodborne":[7],"pathogens":[8,191],"in":[9,76,93,188,199],"the":[10,55,83,124,129,164],"environment":[11],"are":[12],"urgently":[13],"needed":[14],"to":[15,22,53,103,143,159],"curtail":[16],"disease":[17],"outbreaks.":[18],"This":[19,80],"study":[20,81],"aims":[21],"develop":[23],"optimize":[25],"a":[26,77,108,147,178],"fast":[27],"biosensor":[30],"E.":[33,86,168],"coli":[34,87,169],"O157":[35,88],"using":[36],"an":[37],"advanced":[38],"F\u00f6rster":[39],"Resonance":[40],"Energy":[41],"Transfer":[42],"(FRET)":[43],"technology.":[44],"Building":[45],"on":[46,181],"recent":[47],"FRET":[48,153],"technological":[49],"advancements,":[50],"we":[51],"intend":[52],"measure":[54],"degree":[56],"of":[57,85,100,150,166],"interaction":[58],"between":[59],"primary":[60],"antibodies":[61,69],"labeled":[62,70],"with":[63,71],"fluorescein":[64],"isothiocyanate":[65,74],"(FITC)":[66],"secondary":[68],"tetramethyl":[72],"rhodamine":[73],"(TRITC)":[75],"sandwich":[78],"format.":[79],"assesses":[82],"detection":[84,98],"at":[89,111,134],"various":[90],"pH":[91,162,175],"levels":[92],"contaminated":[94],"lettuce":[95],"juice.":[96],"A":[97],"range":[99,149],"10":[101,104],"2":[102],"4":[105],"CFU/mL":[106],"after":[107],"4-hour":[109],"pre-enrichment":[110],"37\u00b0C":[112],"enabled":[113],"quantification.":[115],"In":[116],"this":[117],"FRET-based":[118],"method,":[119],"FITC-IgG":[120],"was":[121,157],"used":[122,158],"as":[123,128],"donor":[125],"TRITC-IgY":[127],"acceptor.":[130],"Samples":[131],"were":[132,139],"stimulated":[133],"350":[135],"nm,":[136,145],"emissions":[138],"measured":[140],"from":[141],"500":[142],"680":[144],"capturing":[146],"wide":[148],"fluorescence":[151,155],"signals.":[152],"microscopic":[154],"imaging":[156],"determine":[160],"how":[161],"affects":[163],"concentration":[165],"live":[167,190],"O157.":[170],"The":[171,183],"results":[172],"showed":[173],"that":[174],"change":[176],"has":[177],"considerable":[179],"impact":[180],"detection.":[182],"approach":[184],"illustrates":[185],"FRET\u2019s":[186],"effectiveness":[187],"identifying":[189],"under":[192],"diverse":[193],"settings,":[194],"indicating":[195],"its":[196],"underlying":[197],"relevance":[198],"pathogen":[200],"identification.":[201]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2025-06-07T00:00:00"}