{"id":"https://openalex.org/W4411054657","doi":"https://doi.org/10.1021/acsinfecdis.5c00147","title":"Isothermal DNA Amplification with Tailed Primers Coupled with Enzyme-Linked Oligonucleotide Assay for the Sensitive Detection of Trichomonas vaginalis","display_name":"Isothermal DNA Amplification with Tailed Primers Coupled with Enzyme-Linked Oligonucleotide Assay for the Sensitive Detection of Trichomonas vaginalis","publication_year":2025,"publication_date":"2025-06-05","ids":{"openalex":"https://openalex.org/W4411054657","doi":"https://doi.org/10.1021/acsinfecdis.5c00147","pmid":"https://pubmed.ncbi.nlm.nih.gov/40472005"},"language":"en","primary_location":{"id":"doi:10.1021/acsinfecdis.5c00147","doi":"https://doi.org/10.1021/acsinfecdis.5c00147","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acsinfecdis.5c00147","pdf_url":null,"source":{"id":"https://openalex.org/S2764646616","display_name":"ACS Infectious Diseases","issn_l":"2373-8227","issn":["2373-8227"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Infectious Diseases","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5050038065","display_name":"Christine Aubrey C. Justo","orcid":"https://orcid.org/0000-0002-9964-592X","observed_orcids":["https://orcid.org/0000-0002-9964-592X"]},"institutions":[{"id":"https://openalex.org/I55952717","display_name":"Universitat Rovira i Virgili","ror":"https://ror.org/00g5sqv46","country_code":"ES","type":"education","lineage":["https://openalex.org/I55952717"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Christine Aubrey C. Justo","raw_affiliation_strings":["Interfibio Consolidated Research Group, Departament d\u2019Enginyeria Qu\u00edmica","Universitat Rovira i Virgili"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Interfibio Consolidated Research Group, Departament d\u2019Enginyeria Qu\u00edmica","institution_ids":[]},{"raw_affiliation_string":"Universitat Rovira i Virgili","institution_ids":["https://openalex.org/I55952717"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031184145","display_name":"Shaira Jane Acosta","orcid":"https://orcid.org/0000-0002-3603-4072","observed_orcids":["https://orcid.org/0000-0002-3603-4072"]},"institutions":[{"id":"https://openalex.org/I55952717","display_name":"Universitat Rovira i Virgili","ror":"https://ror.org/00g5sqv46","country_code":"ES","type":"education","lineage":["https://openalex.org/I55952717"]},{"id":"https://openalex.org/I87074743","display_name":"University of the Philippines Diliman","ror":"https://ror.org/03tbh6y23","country_code":"PH","type":"education","lineage":["https://openalex.org/I103911934","https://openalex.org/I87074743"]}],"countries":["ES","PH"],"is_corresponding":false,"raw_author_name":"Shaira Jane Acosta","raw_affiliation_strings":["Interfibio Consolidated Research Group, Departament d\u2019Enginyeria Qu\u00edmica","Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science","Universitat Rovira i Virgili","University of the Philippines Diliman"],"raw_orcid":"https://orcid.org/0000-0002-3603-4072","affiliations":[{"raw_affiliation_string":"Interfibio Consolidated Research Group, Departament d\u2019Enginyeria Qu\u00edmica","institution_ids":[]},{"raw_affiliation_string":"Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science","institution_ids":[]},{"raw_affiliation_string":"Universitat Rovira i Virgili","institution_ids":["https://openalex.org/I55952717"]},{"raw_affiliation_string":"University of the Philippines Diliman","institution_ids":["https://openalex.org/I87074743"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066068699","display_name":"Miriam Jauset\u2010Rubio","orcid":"https://orcid.org/0000-0002-9943-6132","observed_orcids":["https://orcid.org/0000-0002-9943-6132"]},"institutions":[{"id":"https://openalex.org/I55952717","display_name":"Universitat Rovira i Virgili","ror":"https://ror.org/00g5sqv46","country_code":"ES","type":"education","lineage":["https://openalex.org/I55952717"]}],"countries":["ES"],"is_corresponding":false,"raw_author_name":"Miriam Jauset-Rubio","raw_affiliation_strings":["Interfibio Consolidated Research Group, Departament d\u2019Enginyeria Qu\u00edmica","Universitat Rovira i Virgili"],"raw_orcid":"https://orcid.org/0000-0002-9943-6132","affiliations":[{"raw_affiliation_string":"Interfibio Consolidated Research Group, Departament d\u2019Enginyeria Qu\u00edmica","institution_ids":[]},{"raw_affiliation_string":"Universitat Rovira i Virgili","institution_ids":["https://openalex.org/I55952717"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058538068","display_name":"Vasso Skouridou","orcid":"https://orcid.org/0000-0002-9712-5429","observed_orcids":["https://orcid.org/0000-0002-9712-5429"]},"institutions":[{"id":"https://openalex.org/I55952717","display_name":"Universitat Rovira i Virgili","ror":"https://ror.org/00g5sqv46","country_code":"ES","type":"education","lineage":["https://openalex.org/I55952717"]}],"countries":["ES"],"is_corresponding":true,"raw_author_name":"Vasso Skouridou","raw_affiliation_strings":["Interfibio Consolidated Research Group, Departament d\u2019Enginyeria Qu\u00edmica","Universitat Rovira i Virgili"],"raw_orcid":"https://orcid.org/0000-0002-9712-5429","affiliations":[{"raw_affiliation_string":"Interfibio Consolidated Research Group, Departament d\u2019Enginyeria Qu\u00edmica","institution_ids":[]},{"raw_affiliation_string":"Universitat Rovira i Virgili","institution_ids":["https://openalex.org/I55952717"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088623483","display_name":"Guy Mulumeoderhwa Mulinganya","orcid":"https://orcid.org/0000-0001-7474-4772","observed_orcids":["https://orcid.org/0000-0001-7474-4772"]},"institutions":[{"id":"https://openalex.org/I32597200","display_name":"Ghent University","ror":"https://ror.org/00cv9y106","country_code":"BE","type":"education","lineage":["https://openalex.org/I32597200"]},{"id":"https://openalex.org/I4210124924","display_name":"Universit\u00e9 Catholique de Bukavu","ror":"https://ror.org/03cg80535","country_code":"CD","type":"education","lineage":["https://openalex.org/I4210124924"]}],"countries":["BE","CD"],"is_corresponding":false,"raw_author_name":"Guy Mulinganya","raw_affiliation_strings":["Catholic University of Bukavu","Department of Internal Medicine and Pediatrics, Faculty of Medicine and Health Sciences","Department of Obstetrics and Gynecology","Faculty of Medicine","Ghent University","H\u00f4pital Provincial G\u00e9n\u00e9ral de R\u00e9f\u00e9rence de Bukavu","Department of Internal Medicine and Pediatrics, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium","Faculty of Medicine, Catholic University of Bukavu, Bukavu 019513, Democratic Republic of the Congo"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Catholic University of Bukavu","institution_ids":["https://openalex.org/I4210124924"]},{"raw_affiliation_string":"Department of Internal Medicine and Pediatrics, Faculty of Medicine and Health Sciences","institution_ids":[]},{"raw_affiliation_string":"Department of Obstetrics and Gynecology","institution_ids":[]},{"raw_affiliation_string":"Faculty of Medicine","institution_ids":[]},{"raw_affiliation_string":"Ghent University","institution_ids":["https://openalex.org/I32597200"]},{"raw_affiliation_string":"H\u00f4pital Provincial G\u00e9n\u00e9ral de R\u00e9f\u00e9rence de Bukavu","institution_ids":[]},{"raw_affiliation_string":"Department of Internal Medicine and Pediatrics, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium","institution_ids":["https://openalex.org/I32597200"]},{"raw_affiliation_string":"Faculty of Medicine, Catholic University of Bukavu, Bukavu 019513, Democratic Republic of the Congo","institution_ids":["https://openalex.org/I4210124924"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038809737","display_name":"Piet Cools","orcid":"https://orcid.org/0000-0003-2980-5307","observed_orcids":["https://orcid.org/0000-0003-2980-5307"]},"institutions":[{"id":"https://openalex.org/I32597200","display_name":"Ghent University","ror":"https://ror.org/00cv9y106","country_code":"BE","type":"education","lineage":["https://openalex.org/I32597200"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Piet Cools","raw_affiliation_strings":["Ghent University","Laboratory Bacteriology Research, Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences","Laboratory Bacteriology Research, Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ghent University","institution_ids":["https://openalex.org/I32597200"]},{"raw_affiliation_string":"Laboratory Bacteriology Research, Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences","institution_ids":[]},{"raw_affiliation_string":"Laboratory Bacteriology Research, Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, 9000 Ghent, Belgium","institution_ids":["https://openalex.org/I32597200"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034669769","display_name":"Windell L. Rivera","orcid":"https://orcid.org/0000-0002-4659-6132","observed_orcids":["https://orcid.org/0000-0002-4659-6132"]},"institutions":[{"id":"https://openalex.org/I87074743","display_name":"University of the Philippines Diliman","ror":"https://ror.org/03tbh6y23","country_code":"PH","type":"education","lineage":["https://openalex.org/I103911934","https://openalex.org/I87074743"]}],"countries":["PH"],"is_corresponding":false,"raw_author_name":"Windell L. Rivera","raw_affiliation_strings":["Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science","University of the Philippines Diliman"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science","institution_ids":[]},{"raw_affiliation_string":"University of the Philippines Diliman","institution_ids":["https://openalex.org/I87074743"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5038789374","display_name":"Ciara K. O\u2019Sullivan","orcid":"https://orcid.org/0000-0003-2603-2230","observed_orcids":["https://orcid.org/0000-0003-2603-2230"]},"institutions":[{"id":"https://openalex.org/I11932220","display_name":"Instituci\u00f3 Catalana de Recerca i Estudis Avan\u00e7ats","ror":"https://ror.org/0371hy230","country_code":"ES","type":"nonprofit","lineage":["https://openalex.org/I11932220"]},{"id":"https://openalex.org/I55952717","display_name":"Universitat Rovira i Virgili","ror":"https://ror.org/00g5sqv46","country_code":"ES","type":"education","lineage":["https://openalex.org/I55952717"]}],"countries":["ES"],"is_corresponding":true,"raw_author_name":"Ciara K. O\u2019Sullivan","raw_affiliation_strings":["Instituci\u00f3 Catalana de Recerca I Estudis Avancats (ICREA)","Interfibio Consolidated Research Group, Departament d\u2019Enginyeria Qu\u00edmica","Universitat Rovira i Virgili"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instituci\u00f3 Catalana de Recerca I Estudis Avancats (ICREA)","institution_ids":["https://openalex.org/I11932220"]},{"raw_affiliation_string":"Interfibio Consolidated Research Group, Departament d\u2019Enginyeria Qu\u00edmica","institution_ids":[]},{"raw_affiliation_string":"Universitat Rovira i Virgili","institution_ids":["https://openalex.org/I55952717"]}]}],"institutions":[],"countries_distinct_count":4,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5038789374","https://openalex.org/A5058538068"],"corresponding_institution_ids":["https://openalex.org/I11932220","https://openalex.org/I55952717"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":0.3956,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.45248342,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":95},"biblio":{"volume":"11","issue":"7","first_page":"1944","last_page":"1955"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.9688000082969666,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11393","display_name":"Biosensors and Analytical Detection","score":0.9688000082969666,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10428","display_name":"Reproductive tract infections research","score":0.01810000091791153,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11754","display_name":"SARS-CoV-2 detection and testing","score":0.0044999998062849045,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/trichomonas-vaginalis","display_name":"Trichomonas vaginalis","score":1.0},{"id":"https://openalex.org/keywords/trichomoniasis","display_name":"trichomoniasis","score":0.9269999861717224},{"id":"https://openalex.org/keywords/recombinase-polymerase-amplification","display_name":"recombinase polymerase amplification","score":0.9860000014305115},{"id":"https://openalex.org/keywords/point-of-care-testing","display_name":"point-of-care testing","score":0.7879999876022339},{"id":"https://openalex.org/keywords/dna-dna-hybridization","display_name":"DNA-DNA hybridization","score":0.6669999957084656},{"id":"https://openalex.org/keywords/sexually-transmitted-infections","display_name":"sexually transmitted infections","score":0.6370000243186951},{"id":"https://openalex.org/keywords/molecular-diagnosis","display_name":"molecular diagnosis","score":0.492000013589859},{"id":"https://openalex.org/keywords/detection-limit","display_name":"detection limit","score":0.4779999852180481}],"concepts":[{"id":"https://openalex.org/C139713532","wikidata":"https://www.wikidata.org/wiki/Q17077657","display_name":"Recombinase Polymerase Amplification","level":4,"score":0.9275966882705688},{"id":"https://openalex.org/C2781114625","wikidata":"https://www.wikidata.org/wiki/Q132595","display_name":"Trichomonas vaginalis","level":2,"score":0.8193660378456116},{"id":"https://openalex.org/C60635243","wikidata":"https://www.wikidata.org/wiki/Q6675780","display_name":"Loop-mediated isothermal amplification","level":3,"score":0.6356569528579712},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6307864189147949},{"id":"https://openalex.org/C2777023796","wikidata":"https://www.wikidata.org/wiki/Q745865","display_name":"Trichomoniasis","level":2,"score":0.6028947830200195},{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.599412739276886},{"id":"https://openalex.org/C2777271071","wikidata":"https://www.wikidata.org/wiki/Q422529","display_name":"Nuclease","level":3,"score":0.5512946844100952},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5223984718322754},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5150594711303711},{"id":"https://openalex.org/C156385826","wikidata":"https://www.wikidata.org/wiki/Q1341059","display_name":"Multiple displacement amplification","level":5,"score":0.5142207145690918},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.4766674339771271},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.32725876569747925},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.3227198123931885},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.22540029883384705},{"id":"https://openalex.org/C78063203","wikidata":"https://www.wikidata.org/wiki/Q3062596","display_name":"DNA extraction","level":4,"score":0.15011772513389587},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.06015092134475708},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012680","descriptor_name":"Sensitivity and Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014245","descriptor_name":"Trichomonas Infections","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D014245","descriptor_name":"Trichomonas Infections","qualifier_ui":"Q000469","qualifier_name":"parasitology","is_major_topic":true},{"descriptor_ui":"D014246","descriptor_name":"Trichomonas vaginalis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014246","descriptor_name":"Trichomonas vaginalis","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D014247","descriptor_name":"Trichomonas Vaginitis","qualifier_ui":"Q000175","qualifier_name":"diagnosis","is_major_topic":true},{"descriptor_ui":"D014247","descriptor_name":"Trichomonas Vaginitis","qualifier_ui":"Q000469","qualifier_name":"parasitology","is_major_topic":true},{"descriptor_ui":"D016054","descriptor_name":"DNA, Protozoan","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D021141","descriptor_name":"Nucleic Acid Amplification Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D025202","descriptor_name":"Molecular Diagnostic Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1021/acsinfecdis.5c00147","doi":"https://doi.org/10.1021/acsinfecdis.5c00147","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acsinfecdis.5c00147","pdf_url":null,"source":{"id":"https://openalex.org/S2764646616","display_name":"ACS Infectious Diseases","issn_l":"2373-8227","issn":["2373-8227"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS Infectious Diseases","raw_type":"journal-article"},{"id":"pmid:40472005","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/40472005","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ACS infectious diseases","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.8371999859809875,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G8637558548","display_name":null,"funder_award_id":"FI-SDU 2020 00467","funder_id":"https://openalex.org/F4320334830","funder_display_name":"Ag\u00e8ncia de Gesti\u00f3 d'Ajuts Universitaris i de Recerca"}],"funders":[{"id":"https://openalex.org/F4320324410","display_name":"University of the Philippines","ror":"https://ror.org/02hqkr514"},{"id":"https://openalex.org/F4320334830","display_name":"Ag\u00e8ncia de Gesti\u00f3 d'Ajuts Universitaris i de Recerca","ror":"https://ror.org/01n4pqe45"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":67,"referenced_works":["https://openalex.org/W429140374","https://openalex.org/W2001193282","https://openalex.org/W2019072047","https://openalex.org/W2040454133","https://openalex.org/W2060690767","https://openalex.org/W2065689812","https://openalex.org/W2100727985","https://openalex.org/W2159082815","https://openalex.org/W2163524749","https://openalex.org/W2201650242","https://openalex.org/W2221972471","https://openalex.org/W2258841189","https://openalex.org/W2417360433","https://openalex.org/W2461856757","https://openalex.org/W2470902676","https://openalex.org/W2535112172","https://openalex.org/W2559467066","https://openalex.org/W2573354256","https://openalex.org/W2574248086","https://openalex.org/W2734302978","https://openalex.org/W2754942607","https://openalex.org/W2756684348","https://openalex.org/W2766989075","https://openalex.org/W2789788490","https://openalex.org/W2801687548","https://openalex.org/W2883147421","https://openalex.org/W2883214074","https://openalex.org/W2887827071","https://openalex.org/W2897837407","https://openalex.org/W2919633963","https://openalex.org/W2934740702","https://openalex.org/W2941048908","https://openalex.org/W2949311621","https://openalex.org/W2950484336","https://openalex.org/W3002649546","https://openalex.org/W3005049814","https://openalex.org/W3008086048","https://openalex.org/W3014568282","https://openalex.org/W3014744865","https://openalex.org/W3091025656","https://openalex.org/W3107124276","https://openalex.org/W3134450278","https://openalex.org/W3155582705","https://openalex.org/W3161157739","https://openalex.org/W3175119564","https://openalex.org/W3185444663","https://openalex.org/W3205342107","https://openalex.org/W3208849202","https://openalex.org/W3208924947","https://openalex.org/W4200054899","https://openalex.org/W4210423229","https://openalex.org/W4211093906","https://openalex.org/W4282924061","https://openalex.org/W4283381140","https://openalex.org/W4298132502","https://openalex.org/W4366117435","https://openalex.org/W4382501584","https://openalex.org/W4382700750","https://openalex.org/W4388869227","https://openalex.org/W4390659418","https://openalex.org/W4391173894","https://openalex.org/W4391322771","https://openalex.org/W4394860061","https://openalex.org/W4396814965","https://openalex.org/W4398257326","https://openalex.org/W4398269549","https://openalex.org/W4402065859"],"related_works":["https://openalex.org/W4315651170","https://openalex.org/W4407251480","https://openalex.org/W2469306719","https://openalex.org/W2121212030","https://openalex.org/W3023377850","https://openalex.org/W4402042013","https://openalex.org/W4396872339","https://openalex.org/W2060022105","https://openalex.org/W2160770318","https://openalex.org/W4390659418"],"abstract_inverted_index":{"The":[0,84,159,191,215],"globally":[1],"persistent":[2],"and":[3,29,54,63,79,93,104,146,176,183,231],"most":[4],"common":[5],"sexually":[6],"transmitted":[7],"agent":[8],"Trichomonas":[9],"vaginalis":[10,22,157,230,244],"continues":[11],"to":[12,101,205],"be":[13],"an":[14,30,46],"underestimated":[15],"public":[16],"health":[17],"threat.":[18],"Infection":[19],"with":[20,26,91,198],"T.":[21,73,156,229,243],"has":[23,162],"been":[24],"associated":[25],"reproductive":[27],"complications":[28],"increased":[31],"risk":[32],"of":[33,65,72,86,126,149,154,166,193,239],"HIV":[34],"acquisition.":[35],"With":[36,122],"the":[37,61,69,118,123,127,141,150,155,187,199,210,219],"available":[38],"diagnostic":[39,56],"tools":[40],"for":[41,49,68,144,228,242],"trichomoniasis,":[42],"there":[43],"is":[44,97,177,222],"still":[45],"unmet":[47],"demand":[48],"a":[50,98,163,206,223,234],"cost-effective,":[51],"facile,":[52],"rapid,":[53],"robust":[55],"tool.":[57],"We":[58],"report":[59],"on":[60,117,135],"design":[62],"utilization":[64],"tailed":[66,87,189,220],"primers":[67,221],"molecular":[70,225],"detection":[71,164,226],"vaginalis,":[74],"exploiting":[75,218],"isothermal":[76],"DNA":[77,152],"amplification":[78,107,111,148,185],"solid-phase":[80],"sandwich":[81],"colorimetric":[82],"assay.":[83],"use":[85],"primers,":[88],"in":[89,236],"combination":[90],"enzyme-linked":[92,129],"surface-tethered":[94],"complementary":[95],"probes,":[96],"straightforward":[99],"strategy":[100],"quantitatively":[102],"evaluate":[103],"maximize":[105],"target-specific":[106],"by":[108],"recombinase":[109],"polymerase":[110],"(RPA),":[112],"which":[113],"does":[114],"not":[115],"rely":[116],"typical":[119],"nuclease-probe":[120],"system.":[121],"quantitative":[124,181],"nature":[125],"microplate-based":[128],"oligonucleotide":[130],"assay":[131,208,227],"(ELONA)":[132],"that":[133],"relies":[134],"DNA\u2013DNA":[136],"hybridization,":[137],"we":[138],"systematically":[139],"optimized":[140],"RPA":[142,182],"conditions":[143],"maximal":[145],"specific":[147],"target":[151],"fragment":[153],"genome.":[158],"developed":[160,216],"RPA-ELONA":[161,200,217],"limit":[165],"4.03":[167],"\u00d7":[168],"10":[169],"\u20134":[170],"fg/\u03bcL":[171],"(3.99":[172],"aM)":[173],"synthetic":[174],"dsDNA":[175],"more":[178],"sensitive":[179,224],"than":[180],"PCR-based":[184],"using":[186,209],"same":[188],"primers.":[190],"analysis":[192],"biobanked":[194],"clinical":[195],"genomic":[196],"samples":[197],"showed":[201],"95%":[202],"accuracy":[203],"relative":[204],"qPCR":[207,213],"commercial":[211],"S-DiaMGTV":[212],"kit.":[214],"serves":[232],"as":[233],"guide":[235],"further":[237],"development":[238],"sandwich-based":[240],"POCT":[241],".":[245]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2025-10-10T00:00:00"}