{"id":"https://openalex.org/W4410879367","doi":"https://doi.org/10.1016/j.xpro.2025.103859","title":"Split-and-pool synthesis to generate scalable combinatorial oligonucleotide libraries on magnetic nanoparticles","display_name":"Split-and-pool synthesis to generate scalable combinatorial oligonucleotide libraries on magnetic nanoparticles","publication_year":2025,"publication_date":"2025-05-30","ids":{"openalex":"https://openalex.org/W4410879367","doi":"https://doi.org/10.1016/j.xpro.2025.103859","pmid":"https://pubmed.ncbi.nlm.nih.gov/40449002","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/12166419"},"language":"en","primary_location":{"id":"doi:10.1016/j.xpro.2025.103859","doi":"https://doi.org/10.1016/j.xpro.2025.103859","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xpro.2025.103859","pdf_url":null,"source":{"id":"https://openalex.org/S4210212477","display_name":"STAR Protocols","issn_l":"2666-1667","issn":["2666-1667"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protocols","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1016/j.xpro.2025.103859","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5025638978","display_name":"John V. L. Nguyen","orcid":"https://orcid.org/0000-0003-4492-4910","observed_orcids":["https://orcid.org/0000-0003-4492-4910"]},"institutions":[{"id":"https://openalex.org/I5023651","display_name":"McGill University","ror":"https://ror.org/01pxwe438","country_code":"CA","type":"education","lineage":["https://openalex.org/I5023651"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"John V.L. Nguyen","raw_affiliation_strings":["Department of Biomedical Engineering, McGill University, 3775 University Street, Montreal, QC H3A 2B4, Canada"],"raw_orcid":"https://orcid.org/0000-0003-4492-4910","affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering, McGill University, 3775 University Street, Montreal, QC H3A 2B4, Canada","institution_ids":["https://openalex.org/I5023651"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037214349","display_name":"Lidija Malic","orcid":"https://orcid.org/0000-0001-8594-9191","observed_orcids":["https://orcid.org/0000-0001-8594-9191"]},"institutions":[{"id":"https://openalex.org/I4210159778","display_name":"National Research Council Canada","ror":"https://ror.org/04mte1k06","country_code":"CA","type":"government","lineage":["https://openalex.org/I4210159778"]},{"id":"https://openalex.org/I5023651","display_name":"McGill University","ror":"https://ror.org/01pxwe438","country_code":"CA","type":"education","lineage":["https://openalex.org/I5023651"]}],"countries":["CA"],"is_corresponding":true,"raw_author_name":"Lidija Malic","raw_affiliation_strings":["Department of Biomedical Engineering, McGill University, 3775 University Street, Montreal, QC H3A 2B4, Canada; Medical Devices, Life Sciences Division, National Research Council of Canada, 75 de Mortagne Boulevard, Boucherville, QC J4B 6Y4, Canada. Electronic address: lidija.malic@cnrc-nrc.gc.ca"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering, McGill University, 3775 University Street, Montreal, QC H3A 2B4, Canada; Medical Devices, Life Sciences Division, National Research Council of Canada, 75 de Mortagne Boulevard, Boucherville, QC J4B 6Y4, Canada. Electronic address: lidija.malic@cnrc-nrc.gc.ca","institution_ids":["https://openalex.org/I4210159778","https://openalex.org/I5023651"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086623601","display_name":"Christina Nassif","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210159778","display_name":"National Research Council Canada","ror":"https://ror.org/04mte1k06","country_code":"CA","type":"government","lineage":["https://openalex.org/I4210159778"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Christina Nassif","raw_affiliation_strings":["Medical Devices, Life Sciences Division, National Research Council of Canada, 75 de Mortagne Boulevard, Boucherville, QC J4B 6Y4, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medical Devices, Life Sciences Division, National Research Council of Canada, 75 de Mortagne Boulevard, Boucherville, QC J4B 6Y4, Canada","institution_ids":["https://openalex.org/I4210159778"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043857881","display_name":"Dillon Da Fonte","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210159778","display_name":"National Research Council Canada","ror":"https://ror.org/04mte1k06","country_code":"CA","type":"government","lineage":["https://openalex.org/I4210159778"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Dillon Da Fonte","raw_affiliation_strings":["Medical Devices, Life Sciences Division, National Research Council of Canada, 75 de Mortagne Boulevard, Boucherville, QC J4B 6Y4, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Medical Devices, Life Sciences Division, National Research Council of Canada, 75 de Mortagne Boulevard, Boucherville, QC J4B 6Y4, Canada","institution_ids":["https://openalex.org/I4210159778"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5050052771","display_name":"Maryam Tabrizian","orcid":"https://orcid.org/0000-0002-5050-4480","observed_orcids":["https://orcid.org/0000-0002-5050-4480"]},"institutions":[{"id":"https://openalex.org/I5023651","display_name":"McGill University","ror":"https://ror.org/01pxwe438","country_code":"CA","type":"education","lineage":["https://openalex.org/I5023651"]}],"countries":["CA"],"is_corresponding":true,"raw_author_name":"Maryam Tabrizian","raw_affiliation_strings":["Department of Biomedical Engineering, McGill University, 3775 University Street, Montreal, QC H3A 2B4, Canada; Faculty of Dental Medicine and Oral Health Sciences, McGill University, 2001 McGill College Avenue, Montreal, QC H3A 1G1, Canada. Electronic address: maryam.tabrizian@mcgill.ca"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biomedical Engineering, McGill University, 3775 University Street, Montreal, QC H3A 2B4, Canada; Faculty of Dental Medicine and Oral Health Sciences, McGill University, 2001 McGill College Avenue, Montreal, QC H3A 1G1, Canada. Electronic address: maryam.tabrizian@mcgill.ca","institution_ids":["https://openalex.org/I5023651"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5037214349","https://openalex.org/A5050052771"],"corresponding_institution_ids":["https://openalex.org/I4210159778","https://openalex.org/I5023651"],"apc_list":{"value":1550,"currency":"USD","value_usd":1550},"apc_paid":{"value":1550,"currency":"USD","value_usd":1550},"fwci":0.2208,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":{"value":0.34208554,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":93},"biblio":{"volume":"6","issue":"2","first_page":"103859","last_page":"103859"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.5268999934196472,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10207","display_name":"Advanced biosensing and bioanalysis techniques","score":0.5268999934196472,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.3767000138759613,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.025200000032782555,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/magnetic-nanoparticles","display_name":"magnetic nanoparticles","score":0.9959999918937683},{"id":"https://openalex.org/keywords/oligonucleotide-synthesis","display_name":"oligonucleotide synthesis","score":0.753000020980835},{"id":"https://openalex.org/keywords/dna-sequencing-library-preparation","display_name":"DNA sequencing library preparation","score":0.7200000286102295},{"id":"https://openalex.org/keywords/dna-aptamers","display_name":"DNA aptamers","score":0.7910000085830688},{"id":"https://openalex.org/keywords/dna-origami","display_name":"DNA origami","score":0.7369999885559082},{"id":"https://openalex.org/keywords/synthetic-genome","display_name":"synthetic genome","score":0.843999981880188},{"id":"https://openalex.org/keywords/dna-storage","display_name":"DNA data storage","score":0.6759999990463257}],"concepts":[{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.7896585464477539},{"id":"https://openalex.org/C48044578","wikidata":"https://www.wikidata.org/wiki/Q727490","display_name":"Scalability","level":2,"score":0.6639553904533386},{"id":"https://openalex.org/C155672457","wikidata":"https://www.wikidata.org/wiki/Q61231","display_name":"Nanoparticle","level":2,"score":0.5135829448699951},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5089123249053955},{"id":"https://openalex.org/C87023908","wikidata":"https://www.wikidata.org/wiki/Q3870166","display_name":"Magnetic nanoparticles","level":3,"score":0.49555981159210205},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.46613070368766785},{"id":"https://openalex.org/C2994224535","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial synthesis","level":2,"score":0.4429548680782318},{"id":"https://openalex.org/C171250308","wikidata":"https://www.wikidata.org/wiki/Q11468","display_name":"Nanotechnology","level":1,"score":0.4001632034778595},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.3720017671585083},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3126547932624817},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2365531325340271},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.19620686769485474},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.17196354269981384},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.14411917328834534},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.09113264083862305}],"mesh":[{"descriptor_ui":"D009841","descriptor_name":"Oligonucleotides","qualifier_ui":"Q000138","qualifier_name":"chemical synthesis","is_major_topic":true},{"descriptor_ui":"D009841","descriptor_name":"Oligonucleotides","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D009841","descriptor_name":"Oligonucleotides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D015723","descriptor_name":"Gene Library","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020650","descriptor_name":"Combinatorial Chemistry Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D058185","descriptor_name":"Magnetite Nanoparticles","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":6,"locations":[{"id":"doi:10.1016/j.xpro.2025.103859","doi":"https://doi.org/10.1016/j.xpro.2025.103859","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xpro.2025.103859","pdf_url":null,"source":{"id":"https://openalex.org/S4210212477","display_name":"STAR Protocols","issn_l":"2666-1667","issn":["2666-1667"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protocols","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:12166419","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12166419/","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12166419/pdf/","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protoc","raw_type":"Text"},{"id":"pmh:oai:europepmc.org:10977351","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/12166419","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmid:40449002","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/40449002","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR protocols","raw_type":"Journal Article"},{"id":"pmh:oai:cisti-icist.nrc-cnrc.ca:cistinparc:7c8c6f5c-cfbf-467f-b02e-505571b8d156","doi":null,"is_oa":true,"landing_page_url":"https://nrc-publications.canada.ca/fra/voir/objet/?id=7c8c6f5c-cfbf-467f-b02e-505571b8d156","pdf_url":null,"source":{"id":"https://openalex.org/S7407055245","display_name":"NPARC","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article"},{"id":"pmh:oai:doaj.org/article:1b2128156d2a472986b69b682cb2f44a","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/1b2128156d2a472986b69b682cb2f44a","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"STAR Protocols, Vol 6, Iss 2, Pp 103859- (2025)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1016/j.xpro.2025.103859","doi":"https://doi.org/10.1016/j.xpro.2025.103859","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xpro.2025.103859","pdf_url":null,"source":{"id":"https://openalex.org/S4210212477","display_name":"STAR Protocols","issn_l":"2666-1667","issn":["2666-1667"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protocols","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320310638","display_name":"McGill University","ror":"https://ror.org/01pxwe438"},{"id":"https://openalex.org/F4320320994","display_name":"Canada Research Chairs","ror":"https://ror.org/0517h6h17"},{"id":"https://openalex.org/F4320334593","display_name":"Natural Sciences and Engineering Research Council of Canada","ror":"https://ror.org/01h531d29"},{"id":"https://openalex.org/F4320334601","display_name":"National Research Council Canada","ror":"https://ror.org/04mte1k06"},{"id":"https://openalex.org/F4320334618","display_name":"Fonds de Recherche du Qu\u00e9bec - Sant\u00e9","ror":"https://ror.org/02eqrsj93"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4410879367.pdf","grobid_xml":"https://content.openalex.org/works/W4410879367.grobid-xml"},"referenced_works_count":8,"referenced_works":["https://openalex.org/W1990777342","https://openalex.org/W2053070536","https://openalex.org/W2131470126","https://openalex.org/W2141157874","https://openalex.org/W2153370636","https://openalex.org/W2968499116","https://openalex.org/W3134094763","https://openalex.org/W4405889683"],"related_works":["https://openalex.org/W4405889683","https://openalex.org/W1984992056","https://openalex.org/W2086591786","https://openalex.org/W2289080388","https://openalex.org/W2519547900","https://openalex.org/W4213203727","https://openalex.org/W2060899029","https://openalex.org/W7101097061","https://openalex.org/W73573239","https://openalex.org/W1963513334"],"abstract_inverted_index":{"The":[0],"generation":[1],"of":[2,31,91],"combinatorial":[3,57],"oligonucleotide":[4,33,58],"libraries":[5,23],"is":[6],"desirable":[7],"for":[8,53,71,75],"applications":[9],"such":[10],"as":[11],"DNA":[12,16,81],"aptamers,":[13],"data":[14],"storage,":[15],"origami,":[17],"or":[18],"synthetic":[19],"genomes,":[20],"but":[21],"conventional":[22,76],"present":[24,50],"challenges":[25],"in":[26],"detection":[27],"and":[28,45,77,89],"analysis.":[29],"Synthesis":[30],"unique":[32],"sequences":[34],"on":[35,60,86],"magnetic":[36,61],"nanoparticles":[37,62],"would":[38],"enhance":[39],"the":[40,69,73,87],"ability":[41],"to":[42,96],"manipulate,":[43],"recover,":[44],"detect":[46],"them.":[47],"Here,":[48],"we":[49],"a":[51,55],"protocol":[52],"generating":[54],"scalable":[56],"library":[59,74],"using":[63],"split-and-pool":[64],"synthesis.":[65],"We":[66],"then":[67],"describe":[68],"process":[70],"preparing":[72],"next-generation":[78],"sequencing":[79],"(NGS)":[80],"sequencing.":[82],"For":[83],"complete":[84],"details":[85],"use":[88],"execution":[90],"this":[92],"protocol,":[93],"please":[94],"refer":[95],"Nguyen":[97],"et":[98],"al.":[99],"1":[100]},"counts_by_year":[{"year":2025,"cited_by_count":1}],"updated_date":"2026-11-11T12:16:26.678958","created_date":"2025-10-10T00:00:00"}