{"id":"https://openalex.org/W4409208752","doi":"https://doi.org/10.17504/protocols.io.4r3l29zk4v1y/v1","title":"Protocol forIdentification of Genetic Variation in Genes Linked to Buparvaquone Resistance in Theileria spp. infecting dairy cattle in India v1","display_name":"Protocol forIdentification of Genetic Variation in Genes Linked to Buparvaquone Resistance in Theileria spp. infecting dairy cattle in India v1","publication_year":2025,"publication_date":"2025-02-19","ids":{"openalex":"https://openalex.org/W4409208752","doi":"https://doi.org/10.17504/protocols.io.4r3l29zk4v1y/v1"},"language":"en","primary_location":{"id":"doi:10.17504/protocols.io.4r3l29zk4v1y/v1","doi":"https://doi.org/10.17504/protocols.io.4r3l29zk4v1y/v1","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.4r3l29zk4v1y/v1","pdf_url":"https://www.protocols.io/view/protocol-for-identification-of-genetic-variation-i-d2ab8aan.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.protocols.io/view/protocol-for-identification-of-genetic-variation-i-d2ab8aan.pdf","any_repository_has_fulltext":null},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5114926233","display_name":"Pankaj Musale","orcid":"https://orcid.org/0000-0002-5120-3217","observed_orcids":["https://orcid.org/0000-0002-5120-3217"]},"institutions":[{"id":"https://openalex.org/I3125981224","display_name":"National Chemical Laboratory","ror":"https://ror.org/057mn3690","country_code":"IN","type":"facility","lineage":["https://openalex.org/I2799351866","https://openalex.org/I3125981224","https://openalex.org/I4210134808","https://openalex.org/I66760702"]},{"id":"https://openalex.org/I66760702","display_name":"Council of Scientific and Industrial Research","ror":"https://ror.org/021wm7p51","country_code":"IN","type":"government","lineage":["https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]},{"id":"https://openalex.org/I99364266","display_name":"Academy of Scientific and Innovative Research","ror":"https://ror.org/053rcsq61","country_code":"IN","type":"education","lineage":["https://openalex.org/I99364266"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Pankaj Musale","raw_affiliation_strings":["CSIR National Chemical Laboratory , Biochemical Science Division , Pune , India ; Academy of Scientific and Innovative Research AcSIR , Ghaziabad - 201002 , India;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CSIR National Chemical Laboratory , Biochemical Science Division , Pune , India ; Academy of Scientific and Innovative Research AcSIR , Ghaziabad - 201002 , India;","institution_ids":["https://openalex.org/I3125981224","https://openalex.org/I66760702","https://openalex.org/I99364266"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075650797","display_name":"Ajinkya A. Khilari","orcid":"https://orcid.org/0000-0002-5884-8747","observed_orcids":["https://orcid.org/0000-0002-5884-8747"]},"institutions":[{"id":"https://openalex.org/I3125981224","display_name":"National Chemical Laboratory","ror":"https://ror.org/057mn3690","country_code":"IN","type":"facility","lineage":["https://openalex.org/I2799351866","https://openalex.org/I3125981224","https://openalex.org/I4210134808","https://openalex.org/I66760702"]},{"id":"https://openalex.org/I66760702","display_name":"Council of Scientific and Industrial Research","ror":"https://ror.org/021wm7p51","country_code":"IN","type":"government","lineage":["https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]},{"id":"https://openalex.org/I99364266","display_name":"Academy of Scientific and Innovative Research","ror":"https://ror.org/053rcsq61","country_code":"IN","type":"education","lineage":["https://openalex.org/I99364266"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Ajinkya Khilari","raw_affiliation_strings":["CSIR National Chemical Laboratory , Biochemical Science Division , Pune , India ; Academy of Scientific and Innovative Research AcSIR , Ghaziabad - 201002 , India;"],"raw_orcid":"https://orcid.org/0000-0002-5884-8747","affiliations":[{"raw_affiliation_string":"CSIR National Chemical Laboratory , Biochemical Science Division , Pune , India ; Academy of Scientific and Innovative Research AcSIR , Ghaziabad - 201002 , India;","institution_ids":["https://openalex.org/I3125981224","https://openalex.org/I66760702","https://openalex.org/I99364266"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5114926234","display_name":"Bhagya Turakani","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2800340409","display_name":"BAIF Development Research Foundation","ror":"https://ror.org/05ymegm05","country_code":"IN","type":"other","lineage":["https://openalex.org/I2800340409"]},{"id":"https://openalex.org/I3125981224","display_name":"National Chemical Laboratory","ror":"https://ror.org/057mn3690","country_code":"IN","type":"facility","lineage":["https://openalex.org/I2799351866","https://openalex.org/I3125981224","https://openalex.org/I4210134808","https://openalex.org/I66760702"]},{"id":"https://openalex.org/I66760702","display_name":"Council of Scientific and Industrial Research","ror":"https://ror.org/021wm7p51","country_code":"IN","type":"government","lineage":["https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]},{"id":"https://openalex.org/I99364266","display_name":"Academy of Scientific and Innovative Research","ror":"https://ror.org/053rcsq61","country_code":"IN","type":"education","lineage":["https://openalex.org/I99364266"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Bhagya Turakani","raw_affiliation_strings":["BAIF Development Research Foundation , Animal Breeding Genetics Division , Pune , India","CSIR National Chemical Laboratory , Biochemical Science Division , Pune , India ; Academy of Scientific and Innovative Research AcSIR , Ghaziabad - 201002 , India;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"BAIF Development Research Foundation , Animal Breeding Genetics Division , Pune , India","institution_ids":["https://openalex.org/I2800340409"]},{"raw_affiliation_string":"CSIR National Chemical Laboratory , Biochemical Science Division , Pune , India ; Academy of Scientific and Innovative Research AcSIR , Ghaziabad - 201002 , India;","institution_ids":["https://openalex.org/I3125981224","https://openalex.org/I66760702","https://openalex.org/I99364266"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5065362231","display_name":"Dhanasekaran Shanmugam","orcid":"https://orcid.org/0000-0002-3341-4846","observed_orcids":["https://orcid.org/0000-0002-3341-4846"]},"institutions":[{"id":"https://openalex.org/I3125981224","display_name":"National Chemical Laboratory","ror":"https://ror.org/057mn3690","country_code":"IN","type":"facility","lineage":["https://openalex.org/I2799351866","https://openalex.org/I3125981224","https://openalex.org/I4210134808","https://openalex.org/I66760702"]},{"id":"https://openalex.org/I66760702","display_name":"Council of Scientific and Industrial Research","ror":"https://ror.org/021wm7p51","country_code":"IN","type":"government","lineage":["https://openalex.org/I2799351866","https://openalex.org/I4210134808","https://openalex.org/I66760702"]},{"id":"https://openalex.org/I99364266","display_name":"Academy of Scientific and Innovative Research","ror":"https://ror.org/053rcsq61","country_code":"IN","type":"education","lineage":["https://openalex.org/I99364266"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Dhanasekaran Shanmugam","raw_affiliation_strings":["CSIR National Chemical Laboratory , Biochemical Science Division , Pune , India ; Academy of Scientific and Innovative Research AcSIR , Ghaziabad - 201002 , India;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CSIR National Chemical Laboratory , Biochemical Science Division , Pune , India ; Academy of Scientific and Innovative Research AcSIR , Ghaziabad - 201002 , India;","institution_ids":["https://openalex.org/I3125981224","https://openalex.org/I66760702","https://openalex.org/I99364266"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10296","display_name":"Vector-borne infectious diseases","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10296","display_name":"Vector-borne infectious diseases","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11074","display_name":"Helminth infection and control","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11688","display_name":"Microbial infections and disease research","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bovine-theileriosis","display_name":"bovine theileriosis","score":0.9350000023841858},{"id":"https://openalex.org/keywords/theileria-annulata","display_name":"Theileria annulata","score":0.9980000257492065},{"id":"https://openalex.org/keywords/theileria-orientalis","display_name":"Theileria orientalis","score":1.0},{"id":"https://openalex.org/keywords/genetic-variation","display_name":"genetic variation","score":0.7829999923706055},{"id":"https://openalex.org/keywords/cytochrome-b","display_name":"cytochrome b","score":0.8399999737739563},{"id":"https://openalex.org/keywords/dihydroorotate-dehydrogenase","display_name":"dihydroorotate dehydrogenase","score":0.9940000176429749},{"id":"https://openalex.org/keywords/peptidyl-prolyl-cis-trans-isomerase","display_name":"peptidyl-prolyl isomerase","score":0.9929999709129333},{"id":"https://openalex.org/keywords/multiplex-pcr","display_name":"multiplex PCR","score":0.8690000176429749},{"id":"https://openalex.org/keywords/oxford-nanopore-sequencing","display_name":"Oxford Nanopore sequencing","score":0.8920000195503235},{"id":"https://openalex.org/keywords/species-detection","display_name":"species detection","score":0.6949999928474426},{"id":"https://openalex.org/keywords/tick-borne-disease","display_name":"tick-borne diseases","score":0.8130000233650208},{"id":"https://openalex.org/keywords/dairy-cows","display_name":"dairy cattle","score":0.9129999876022339}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6491740942001343},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5233007073402405},{"id":"https://openalex.org/C68873052","wikidata":"https://www.wikidata.org/wiki/Q349856","display_name":"Genetic variation","level":3,"score":0.48482051491737366},{"id":"https://openalex.org/C42972112","wikidata":"https://www.wikidata.org/wiki/Q170201","display_name":"Veterinary medicine","level":1,"score":0.4803396463394165},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3854001760482788},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.3486494719982147},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.15181121230125427}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.17504/protocols.io.4r3l29zk4v1y/v1","doi":"https://doi.org/10.17504/protocols.io.4r3l29zk4v1y/v1","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.4r3l29zk4v1y/v1","pdf_url":"https://www.protocols.io/view/protocol-for-identification-of-genetic-variation-i-d2ab8aan.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.17504/protocols.io.4r3l29zk4v1y/v1","doi":"https://doi.org/10.17504/protocols.io.4r3l29zk4v1y/v1","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.4r3l29zk4v1y/v1","pdf_url":"https://www.protocols.io/view/protocol-for-identification-of-genetic-variation-i-d2ab8aan.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.4000000059604645}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320311347","display_name":"BAIF Development Research Foundation","ror":"https://ror.org/05ymegm05"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4409208752.pdf","grobid_xml":"https://content.openalex.org/works/W4409208752.grobid-xml"},"referenced_works_count":1,"referenced_works":["https://openalex.org/W2989963470"],"related_works":["https://openalex.org/W4404897269","https://openalex.org/W4412493732","https://openalex.org/W4413056511","https://openalex.org/W4411878576","https://openalex.org/W4293796553","https://openalex.org/W4385899110","https://openalex.org/W4282039289","https://openalex.org/W6944008525","https://openalex.org/W3003692145","https://openalex.org/W4313485091"],"abstract_inverted_index":{"Buparvaquone":[0],"(BQO)":[1],"is":[2,78],"the":[3,67],"primary":[4],"drug":[5],"used":[6],"to":[7],"treat":[8],"bovine":[9,90],"theileriosis,":[10],"a":[11,24],"tick-borne":[12],"disease":[13],"caused":[14],"by":[15,49],"T.":[16,19,44,47],"annulata":[17,45],"and":[18,26,39,46,60,65,75,85],"orientalis.":[20],"This":[21],"protocol":[22],"establishes":[23],"sensitive":[25],"rapid":[27],"multiplex":[28],"PCR-based":[29],"method,":[30],"combined":[31],"with":[32],"Oxford":[33],"nanopore":[34],"sequencing,":[35],"for":[36,80,89],"species":[37],"detection":[38],"BQO":[40,76],"resistance":[41,77,83],"profiling":[42],"of":[43],"orientalis":[48],"targeting":[50],"three":[51],"key":[52],"genes:":[53],"cytochrome":[54],"b":[55],"(cytb),":[56],"dihydroorotate":[57],"dehydrogenase":[58],"(dhodh),":[59],"peptidyl-prolyl":[61],"isomerase":[62],"(pin1),":[63],"Monitoring":[64],"establishing":[66],"link":[68],"between":[69],"genetic":[70],"variations":[71],"in":[72],"these":[73],"genes":[74],"crucial":[79],"understanding":[81],"emerging":[82],"patterns":[84],"improving":[86],"treatment":[87],"strategies":[88],"theileriosis.":[91]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2025-04-07T00:00:00"}