{"id":"https://openalex.org/W4408734305","doi":"https://doi.org/10.71336/jabs.968","title":"IN VITRO AND IN SILICO CYTOTOXICITY EVALUATION OF SOME ISATIN MANNICH BASES ON HUMAN MELANOMA CELLS","display_name":"IN VITRO AND IN SILICO CYTOTOXICITY EVALUATION OF SOME ISATIN MANNICH BASES ON HUMAN MELANOMA CELLS","publication_year":2022,"publication_date":"2022-01-23","ids":{"openalex":"https://openalex.org/W4408734305","doi":"https://doi.org/10.71336/jabs.968"},"language":"en","primary_location":{"id":"doi:10.71336/jabs.968","doi":"https://doi.org/10.71336/jabs.968","is_oa":true,"landing_page_url":"https://doi.org/10.71336/jabs.968","pdf_url":"https://jabsonline.org/index.php/jabs/article/download/968/688","source":{"id":"https://openalex.org/S5406978913","display_name":"Journal of Applied Biological Sciences","issn_l":"2146-0108","issn":["2146-0108"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["norway-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Applied Biological Sciences","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","datacite"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://jabsonline.org/index.php/jabs/article/download/968/688","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Mehmet Mustafa Isgor","orcid":"https://orcid.org/0000-0002-1937-2897","observed_orcids":["https://orcid.org/0000-0002-1937-2897"]},"institutions":[{"id":"https://openalex.org/I46000314","display_name":"Mustafa Kemal University","ror":"https://ror.org/056hcgc41","country_code":"TR","type":"education","lineage":["https://openalex.org/I46000314"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Mehmet Mustafa Isgor","raw_affiliation_strings":["Department of Biochemistry, Faculty of Veterinary, Hatay Mustafa Kemal University, Hatay, TURKEY"],"raw_orcid":"https://orcid.org/0000-0002-1937-2897","affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Faculty of Veterinary, Hatay Mustafa Kemal University, Hatay, TURKEY","institution_ids":["https://openalex.org/I46000314"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063905013","display_name":"Altu\u011f K\u00fc\u00e7\u00fckg\u00fcl","orcid":"https://orcid.org/0000-0003-4387-6814","observed_orcids":["https://orcid.org/0000-0003-4387-6814"]},"institutions":[{"id":"https://openalex.org/I46000314","display_name":"Mustafa Kemal University","ror":"https://ror.org/056hcgc41","country_code":"TR","type":"education","lineage":["https://openalex.org/I46000314"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Altug Kucukgul","raw_affiliation_strings":["Hatay Mustafa Kemal University , Veterinary Faculty , Department of Biochemistry , Hatay , Turkey"],"raw_orcid":"https://orcid.org/0000-0003-4387-6814","affiliations":[{"raw_affiliation_string":"Hatay Mustafa Kemal University , Veterinary Faculty , Department of Biochemistry , Hatay , Turkey","institution_ids":["https://openalex.org/I46000314"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Muhammet Karaman","orcid":"https://orcid.org/0000-0001-5700-0835","observed_orcids":["https://orcid.org/0000-0001-5700-0835"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Muhammet Karaman","raw_affiliation_strings":["Kilis"],"raw_orcid":"https://orcid.org/0000-0001-5700-0835","affiliations":[{"raw_affiliation_string":"Kilis","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"Dilan Ozmen Ozgun","orcid":"https://orcid.org/0000-0002-1145-8186","observed_orcids":["https://orcid.org/0000-0002-1145-8186"]},"institutions":[{"id":"https://openalex.org/I307617284","display_name":"A\u011fr\u0131 \u0130brahim \u00c7e\u00e7en University","ror":"https://ror.org/054y2mb78","country_code":"TR","type":"education","lineage":["https://openalex.org/I307617284"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Dilan Ozmen Ozgun","raw_affiliation_strings":["Ibrahim Cecen University , Faculty of Pharmacy , Department of Pharmaceutical Chemistry , A\u011fr\u0131 , Turkey"],"raw_orcid":"https://orcid.org/0000-0002-1145-8186","affiliations":[{"raw_affiliation_string":"Ibrahim Cecen University , Faculty of Pharmacy , Department of Pharmaceutical Chemistry , A\u011fr\u0131 , Turkey","institution_ids":["https://openalex.org/I307617284"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078613631","display_name":"Telat Yan\u0131k","orcid":"https://orcid.org/0000-0003-0975-0281","observed_orcids":["https://orcid.org/0000-0003-0975-0281"]},"institutions":[{"id":"https://openalex.org/I64134780","display_name":"Atat\u00fcrk University","ror":"https://ror.org/03je5c526","country_code":"TR","type":"education","lineage":["https://openalex.org/I64134780"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Telat Yanik","raw_affiliation_strings":["Ataturk University , Faculty of Fisheries , Department of Aquaculture , Erzurum , Turkey"],"raw_orcid":"https://orcid.org/0000-0003-0975-0281","affiliations":[{"raw_affiliation_string":"Ataturk University , Faculty of Fisheries , Department of Aquaculture , Erzurum , Turkey","institution_ids":["https://openalex.org/I64134780"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014667226","display_name":"Halise Inci Gul","orcid":"https://orcid.org/0000-0001-6164-9602","observed_orcids":["https://orcid.org/0000-0001-6164-9602"]},"institutions":[{"id":"https://openalex.org/I4210151619","display_name":"Kilis 7 Aral\u0131k University","ror":"https://ror.org/048b6qs33","country_code":"TR","type":"education","lineage":["https://openalex.org/I4210151619"]},{"id":"https://openalex.org/I64134780","display_name":"Atat\u00fcrk University","ror":"https://ror.org/03je5c526","country_code":"TR","type":"education","lineage":["https://openalex.org/I64134780"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Halise Inci Gul","raw_affiliation_strings":["Aral\u0131k University , Faculty of Arts and Science , Department of Mol. Biology and Genetics , Kilis , Turkey","Atat\u00fcrk University , Faculty of Pharmacy , Department of Pharmaceutical Chemistry , Erzurum , Turkey"],"raw_orcid":"https://orcid.org/0000-0001-6164-9602","affiliations":[{"raw_affiliation_string":"Aral\u0131k University , Faculty of Arts and Science , Department of Mol. Biology and Genetics , Kilis , Turkey","institution_ids":["https://openalex.org/I4210151619"]},{"raw_affiliation_string":"Atat\u00fcrk University , Faculty of Pharmacy , Department of Pharmaceutical Chemistry , Erzurum , Turkey","institution_ids":["https://openalex.org/I64134780"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5116748663","display_name":"Mehtap Bulgurcu","orcid":"https://orcid.org/0000-0002-1063-386X","observed_orcids":["https://orcid.org/0000-0002-1063-386X"]},"institutions":[{"id":"https://openalex.org/I46000314","display_name":"Mustafa Kemal University","ror":"https://ror.org/056hcgc41","country_code":"TR","type":"education","lineage":["https://openalex.org/I46000314"]}],"countries":["TR"],"is_corresponding":false,"raw_author_name":"Mehtap Bulgurcu","raw_affiliation_strings":["Hatay Mustafa Kemal University , Veterinary Faculty , Department of Biochemistry , Hatay , Turkey"],"raw_orcid":"https://orcid.org/0000-0002-1063-386X","affiliations":[{"raw_affiliation_string":"Hatay Mustafa Kemal University , Veterinary Faculty , Department of Biochemistry , Hatay , Turkey","institution_ids":["https://openalex.org/I46000314"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.1132667,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"16","issue":"1","first_page":"102","last_page":"114"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10274","display_name":"Heterocyclic Compound Synthesis and Bioactivity","score":0.9560999870300293,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10274","display_name":"Heterocyclic Compound Synthesis and Bioactivity","score":0.9560999870300293,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12356","display_name":"Bioactive Compounds and Antitumor Agents","score":0.01640000008046627,"subfield":{"id":"https://openalex.org/subfields/3005","display_name":"Toxicology"},"field":{"id":"https://openalex.org/fields/30","display_name":"Pharmacology, Toxicology and Pharmaceutics"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10163","display_name":"Metal complexes synthesis and properties","score":0.01080000028014183,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/melanoma","display_name":"melanoma","score":0.6759999990463257},{"id":"https://openalex.org/keywords/g361-cells","display_name":"G361 cells","score":0.8450000286102295},{"id":"https://openalex.org/keywords/cytotoxicity","display_name":"cytotoxicity","score":0.9539999961853027},{"id":"https://openalex.org/keywords/morpholine","display_name":"morpholine","score":0.7149999737739563},{"id":"https://openalex.org/keywords/piperidine","display_name":"piperidine","score":0.9750000238418579},{"id":"https://openalex.org/keywords/n-methylpiperazine","display_name":"N-methylpiperazine","score":0.9779999852180481},{"id":"https://openalex.org/keywords/caspase-3","display_name":"caspase-3","score":0.9369999766349792},{"id":"https://openalex.org/keywords/p53","display_name":"p53","score":0.8830000162124634},{"id":"https://openalex.org/keywords/anticancer-activity","display_name":"anticancer activity","score":0.75},{"id":"https://openalex.org/keywords/allosteric-activation","display_name":"allosteric activation","score":0.7269999980926514},{"id":"https://openalex.org/keywords/molecular-docking","display_name":"molecular docking","score":0.6589999794960022},{"id":"https://openalex.org/keywords/metal-chelation","display_name":"metal chelation","score":0.843999981880188}],"concepts":[{"id":"https://openalex.org/C2777256644","wikidata":"https://www.wikidata.org/wiki/Q421348","display_name":"Isatin","level":2,"score":0.9741603136062622},{"id":"https://openalex.org/C2775905019","wikidata":"https://www.wikidata.org/wiki/Q192572","display_name":"In silico","level":3,"score":0.8580329418182373},{"id":"https://openalex.org/C109316439","wikidata":"https://www.wikidata.org/wiki/Q246181","display_name":"Cytotoxicity","level":3,"score":0.8125443458557129},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.7545449733734131},{"id":"https://openalex.org/C2777658100","wikidata":"https://www.wikidata.org/wiki/Q180614","display_name":"Melanoma","level":2,"score":0.668055534362793},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.561582088470459},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3709721565246582},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.32891181111335754},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.28096669912338257},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.2230018675327301},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.16191142797470093},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.71336/jabs.968","doi":"https://doi.org/10.71336/jabs.968","is_oa":true,"landing_page_url":"https://doi.org/10.71336/jabs.968","pdf_url":"https://jabsonline.org/index.php/jabs/article/download/968/688","source":{"id":"https://openalex.org/S5406978913","display_name":"Journal of Applied Biological Sciences","issn_l":"2146-0108","issn":["2146-0108"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["norway-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Applied Biological Sciences","raw_type":"journal-article"},{"id":"pmh:oai:oai.jabsonline.org:article/968","doi":null,"is_oa":true,"landing_page_url":"https://jabsonline.org/index.php/jabs/article/view/968","pdf_url":null,"source":{"id":"https://openalex.org/S5406978913","display_name":"Journal of Applied Biological Sciences","issn_l":"2146-0108","issn":["2146-0108"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["norway-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"2146-0108","raw_type":"info:eu-repo/semantics/article"},{"id":"doi:10.5281/zenodo.5826185","doi":"https://doi.org/10.5281/zenodo.5826185","is_oa":true,"landing_page_url":"https://doi.org/10.5281/zenodo.5826185","pdf_url":null,"source":{"id":"https://openalex.org/S4306400562","display_name":"Zenodo (CERN European Organization for Nuclear Research)","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I67311998","host_organization_name":"European Organization for Nuclear Research","host_organization_lineage":["https://openalex.org/I67311998"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"JournalArticle"},{"id":"doi:10.5281/zenodo.5826186","doi":"https://doi.org/10.5281/zenodo.5826186","is_oa":true,"landing_page_url":"https://doi.org/10.5281/zenodo.5826186","pdf_url":null,"source":{"id":"https://openalex.org/S4306400562","display_name":"Zenodo (CERN European Organization for Nuclear Research)","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I67311998","host_organization_name":"European Organization for Nuclear Research","host_organization_lineage":["https://openalex.org/I67311998"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"JournalArticle"}],"best_oa_location":{"id":"doi:10.71336/jabs.968","doi":"https://doi.org/10.71336/jabs.968","is_oa":true,"landing_page_url":"https://doi.org/10.71336/jabs.968","pdf_url":"https://jabsonline.org/index.php/jabs/article/download/968/688","source":{"id":"https://openalex.org/S5406978913","display_name":"Journal of Applied Biological Sciences","issn_l":"2146-0108","issn":["2146-0108"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":["norway-1"],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Applied Biological Sciences","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.9476000070571899,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4408734305.pdf","grobid_xml":"https://content.openalex.org/works/W4408734305.grobid-xml"},"referenced_works_count":47,"referenced_works":["https://openalex.org/W1155563270","https://openalex.org/W1595856931","https://openalex.org/W1607548879","https://openalex.org/W1879685682","https://openalex.org/W1939411505","https://openalex.org/W1965086052","https://openalex.org/W1979153024","https://openalex.org/W1987489104","https://openalex.org/W1988876535","https://openalex.org/W1990177961","https://openalex.org/W2003436246","https://openalex.org/W2028699785","https://openalex.org/W2034496688","https://openalex.org/W2038444562","https://openalex.org/W2044854137","https://openalex.org/W2048441067","https://openalex.org/W2052576351","https://openalex.org/W2055656744","https://openalex.org/W2058964568","https://openalex.org/W2068264087","https://openalex.org/W2072613417","https://openalex.org/W2078783291","https://openalex.org/W2081145901","https://openalex.org/W2086514037","https://openalex.org/W2093076262","https://openalex.org/W2094375196","https://openalex.org/W2113468468","https://openalex.org/W2123184604","https://openalex.org/W2129191108","https://openalex.org/W2130892478","https://openalex.org/W2133959309","https://openalex.org/W2288982338","https://openalex.org/W2325707043","https://openalex.org/W2336869895","https://openalex.org/W2353789499","https://openalex.org/W2487649280","https://openalex.org/W2726205423","https://openalex.org/W2746996444","https://openalex.org/W2764055019","https://openalex.org/W2773546291","https://openalex.org/W2905303571","https://openalex.org/W2908955427","https://openalex.org/W2949154755","https://openalex.org/W2954402636","https://openalex.org/W2967836987","https://openalex.org/W2985397811","https://openalex.org/W2990795604"],"related_works":["https://openalex.org/W3119251901","https://openalex.org/W1878707781","https://openalex.org/W2087605103","https://openalex.org/W7165554881","https://openalex.org/W2000704821","https://openalex.org/W2059493536","https://openalex.org/W1987489104","https://openalex.org/W2052087590","https://openalex.org/W1980813169","https://openalex.org/W6983336536"],"abstract_inverted_index":{"Mannich":[0,106,165],"bases":[1,21,107],"tend":[2],"to":[3,77,81,152,171,200],"suppress":[4],"cell":[5],"proliferation":[6],"in":[7,60,138,194],"damaged":[8],"tissues":[9],"because":[10],"of":[11,18,94,187,206],"particular":[12],"metal":[13],"chelation":[14],"properties.":[15],"Amine":[16],"components":[17],"isatin":[19],"mannich":[20],"-":[22],"piperidine":[23],"(P1),":[24],"morpholine":[25],"(P2)":[26],"and":[27,43,50,70,97,113,120,197],"N-methyl":[28],"piperazine":[29],"(P3)":[30],"\u2013":[31],"were":[32,46,58,75,99,108,169],"evaluated":[33],"for":[34,89,117,193],"their":[35],"cytotoxicity":[36],"potentials":[37],"on":[38],"melanoma":[39,55,87],"cells.":[40],"P1,":[41,118],"P2":[42,119,123,146,155,181],"P3":[44],"products":[45],"purified":[47],"using":[48],"crystallization":[49],"characterized":[51],"by":[52,104,145,160],"NMR.":[53],"Human":[54],"cells":[56],"(G361)":[57],"produced":[59],"DMEM":[61],"medium":[62,78],"including":[63],"10%":[64],"FBS,":[65],"1%":[66],"penicillin/streptomycin":[67],"at":[68,110],"37\u00b0C":[69],"5%":[71],"CO2":[72],"conditions.":[73],"Compounds":[74],"applied":[76],"containers":[79],"separated":[80],"24":[82,90],"pieces":[83],"plates":[84],"as":[85],"1x105":[86],"cells/well":[88],"hours.":[91],"Expression":[92],"levels":[93],"caspase-3,":[95],"p53":[96,139],"\u03b2-actin":[98],"investigated":[100],"from":[101],"RNA":[102],"samples":[103],"qRT-PCR.":[105],"efficient":[109],"20,":[111],"20":[112],"50":[114],"\u03bcg/ml":[115],"concentrations":[116],"P3,":[121],"respectively.":[122],"(20":[124],"\u03bcg/ml)":[125],"showed":[126],"the":[127,203],"highest":[128],"cytotoxic":[129],"effect":[130],"with":[131,148],"92":[132],"percent.":[133],"The":[134],"most":[135],"significant":[136],"increase":[137],"gene":[140],"expression":[141,159],"was":[142],"carried":[143],"out":[144],"product":[147],"6.78":[149],"fold":[150],"compared":[151],"control":[153],"group.":[154],"also":[156],"upregulated":[157],"caspase-3":[158],"9.72":[161],"fold.":[162],"Newly":[163],"synthesized":[164],"bases,":[166],"especially":[167],"P2,":[168],"found":[170],"have":[172],"antitumor":[173],"potential.":[174],"Moreover,":[175],"molecular":[176,204],"docking":[177],"studies":[178],"revealed":[179],"that":[180],"is":[182,191],"a":[183],"potent":[184],"allosteric":[185],"activator":[186],"caspase-3.":[188],"However,":[189],"there":[190],"need":[192],"vivo":[195],"trials":[196],"extensive":[198],"researches":[199],"fully":[201],"elucidate":[202],"bioefficacies":[205],"these":[207],"molecules.":[208]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2025-03-23T00:00:00"}