{"id":"https://openalex.org/W4408201284","doi":"https://doi.org/10.1021/acs.biochem.4c00717","title":"Alanine Scanning to Define Membrane Protein\u2013Lipid Interaction Sites Using Native Mass Spectrometry","display_name":"Alanine Scanning to Define Membrane Protein\u2013Lipid Interaction Sites Using Native Mass Spectrometry","publication_year":2025,"publication_date":"2025-03-06","ids":{"openalex":"https://openalex.org/W4408201284","doi":"https://doi.org/10.1021/acs.biochem.4c00717","pmid":"https://pubmed.ncbi.nlm.nih.gov/39484449"},"language":"en","primary_location":{"id":"doi:10.1021/acs.biochem.4c00717","doi":"https://doi.org/10.1021/acs.biochem.4c00717","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.biochem.4c00717","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.1101/2024.10.24.620105","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5030290236","display_name":"Hiruni S. Jayasekera","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hiruni S. Jayasekera","raw_affiliation_strings":["Department of Chemistry and Biochemistry","University of Arizona","Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 85721, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry","institution_ids":[]},{"raw_affiliation_string":"University of Arizona","institution_ids":["https://openalex.org/I138006243"]},{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 85721, United States","institution_ids":["https://openalex.org/I138006243"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5083941085","display_name":"Farhana Afrin Mohona","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Farhana Afrin Mohona","raw_affiliation_strings":["Department of Chemistry and Biochemistry","University of Arizona","Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 85721, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry","institution_ids":[]},{"raw_affiliation_string":"University of Arizona","institution_ids":["https://openalex.org/I138006243"]},{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 85721, United States","institution_ids":["https://openalex.org/I138006243"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5115597033","display_name":"Madison J. De Jesus","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Madison J. De Jesus","raw_affiliation_strings":["Department of Chemistry and Biochemistry","University of Arizona","Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 85721, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry","institution_ids":[]},{"raw_affiliation_string":"University of Arizona","institution_ids":["https://openalex.org/I138006243"]},{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 85721, United States","institution_ids":["https://openalex.org/I138006243"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Katherine M. Miller","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Katherine M. Miller","raw_affiliation_strings":["Department of Chemistry and Biochemistry","University of Arizona","Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 85721, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry","institution_ids":[]},{"raw_affiliation_string":"University of Arizona","institution_ids":["https://openalex.org/I138006243"]},{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 85721, United States","institution_ids":["https://openalex.org/I138006243"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5089783459","display_name":"Michael Thomas Marty","orcid":"https://orcid.org/0000-0001-8115-1772","observed_orcids":["https://orcid.org/0000-0001-8115-1772"]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Michael T. Marty","raw_affiliation_strings":["Department of Chemistry and Biochemistry","University of Arizona","Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 85721, United States"],"raw_orcid":"https://orcid.org/0000-0001-8115-1772","affiliations":[{"raw_affiliation_string":"Department of Chemistry and Biochemistry","institution_ids":[]},{"raw_affiliation_string":"University of Arizona","institution_ids":["https://openalex.org/I138006243"]},{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Arizona, Tucson, Arizona 85721, United States","institution_ids":["https://openalex.org/I138006243"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5089783459"],"corresponding_institution_ids":["https://openalex.org/I138006243"],"apc_list":{"value":4500,"currency":"USD","value_usd":4500},"apc_paid":null,"fwci":1.0101,"has_fulltext":true,"cited_by_count":4,"citation_normalized_percentile":{"value":0.71209641,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":97},"biblio":{"volume":"64","issue":"6","first_page":"1308","last_page":"1316"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.760699987411499,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10407","display_name":"Lipid Membrane Structure and Behavior","score":0.760699987411499,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.2272000014781952,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.009200000204145908,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aquaporin-z","display_name":"aquaporin Z","score":0.9240000247955322},{"id":"https://openalex.org/keywords/aquaporins","display_name":"aquaporins","score":0.8289999961853027},{"id":"https://openalex.org/keywords/lipid-binding-sites","display_name":"lipid binding sites","score":0.7129999995231628},{"id":"https://openalex.org/keywords/membrane-protein-lipid-interactions","display_name":"membrane protein-lipid interactions","score":0.7850000262260437},{"id":"https://openalex.org/keywords/cardiolipin","display_name":"cardiolipin","score":0.824999988079071},{"id":"https://openalex.org/keywords/lipid-selectivity","display_name":"lipid selectivity","score":0.9399999976158142},{"id":"https://openalex.org/keywords/alanine-scanning","display_name":"alanine scanning","score":0.8059999942779541},{"id":"https://openalex.org/keywords/hydrophobic-pocket","display_name":"hydrophobic pocket","score":0.7929999828338623},{"id":"https://openalex.org/keywords/lipid-orientation","display_name":"lipid orientation","score":0.5070000290870667},{"id":"https://openalex.org/keywords/protein-structure-mapping","display_name":"protein structure mapping","score":0.578000009059906},{"id":"https://openalex.org/keywords/molecular-dynamics-simulation","display_name":"molecular dynamics simulation","score":0.5460000038146973}],"concepts":[{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.7247962951660156},{"id":"https://openalex.org/C2779856020","wikidata":"https://www.wikidata.org/wiki/Q218642","display_name":"Alanine","level":3,"score":0.6300387382507324},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6225857138633728},{"id":"https://openalex.org/C144647389","wikidata":"https://www.wikidata.org/wiki/Q423042","display_name":"Membrane protein","level":3,"score":0.4449999928474426},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.4140971302986145},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.35091519355773926},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.33000418543815613},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.1502721905708313}],"mesh":[],"locations_count":6,"locations":[{"id":"doi:10.1021/acs.biochem.4c00717","doi":"https://doi.org/10.1021/acs.biochem.4c00717","is_oa":false,"landing_page_url":"https://doi.org/10.1021/acs.biochem.4c00717","pdf_url":null,"source":{"id":"https://openalex.org/S104038378","display_name":"Biochemistry","issn_l":"0006-2960","issn":["0006-2960","1520-4995","1943-295X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320006","host_organization_name":"American Chemical Society","host_organization_lineage":["https://openalex.org/P4310320006"],"host_organization_lineage_names":["American Chemical Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"journal-article"},{"id":"doi:10.1101/2024.10.24.620105","doi":"https://doi.org/10.1101/2024.10.24.620105","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2024.10.24.620105","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:39484449","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/39484449","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"bioRxiv : the preprint server for biology","raw_type":"Journal Article"},{"id":"pmid:40047061","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/40047061","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biochemistry","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:11527333","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11527333","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11527333/pdf/nihpp-2024.10.24.620105v2.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:11919553","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11919553/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Biochemistry","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1101/2024.10.24.620105","doi":"https://doi.org/10.1101/2024.10.24.620105","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2024.10.24.620105","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G215083959","display_name":null,"funder_award_id":"R35GM128624","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G6832830247","display_name":null,"funder_award_id":"RM1GM145416","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":37,"referenced_works":["https://openalex.org/W1966078827","https://openalex.org/W1976484542","https://openalex.org/W1990771438","https://openalex.org/W2002508442","https://openalex.org/W2008887848","https://openalex.org/W2021520922","https://openalex.org/W2023979176","https://openalex.org/W2024609981","https://openalex.org/W2029667189","https://openalex.org/W2030370943","https://openalex.org/W2044143372","https://openalex.org/W2121501020","https://openalex.org/W2134762386","https://openalex.org/W2150981663","https://openalex.org/W2171268876","https://openalex.org/W2172834637","https://openalex.org/W2290785648","https://openalex.org/W2334348545","https://openalex.org/W2346089478","https://openalex.org/W2469172798","https://openalex.org/W2558396410","https://openalex.org/W2578365017","https://openalex.org/W2899955103","https://openalex.org/W2905876773","https://openalex.org/W2955844986","https://openalex.org/W3004520297","https://openalex.org/W3046751266","https://openalex.org/W3096559291","https://openalex.org/W3119203511","https://openalex.org/W3157814829","https://openalex.org/W3195672811","https://openalex.org/W4205616329","https://openalex.org/W4281622997","https://openalex.org/W4308635391","https://openalex.org/W4386695301","https://openalex.org/W4391487061","https://openalex.org/W4399513715"],"related_works":["https://openalex.org/W2150391754","https://openalex.org/W2064710306","https://openalex.org/W1997484252","https://openalex.org/W1979156653","https://openalex.org/W2249456921","https://openalex.org/W2184834470","https://openalex.org/W2334502792","https://openalex.org/W2133255487","https://openalex.org/W4244927301","https://openalex.org/W4206791651"],"abstract_inverted_index":{"Lipids":[0],"surrounding":[1],"membrane":[2,31,37],"proteins":[3],"interact":[4,174],"with":[5,11,134,163,175],"different":[6,114,122],"sites":[7,108,198],"on":[8,216],"the":[9,25,47,53,81,119,167,181,185,200,211,217],"protein":[10,38,213],"varying":[12],"specificities,":[13],"ranging":[14],"from":[15],"highly":[16],"specific":[17,84,105,158],"to":[18,34,45,79,103,117,139,209],"weak":[19],"interactions.":[20],"These":[21],"interactions":[22,142],"can":[23],"modulate":[24],"structure,":[26],"function,":[27,41],"and":[28,40,52,74,109,170,199],"stability":[29],"of":[30,49,56,83,99,101,113,121,202],"proteins.":[32],"Thus,":[33],"better":[35],"understand":[36],"structure":[39,214],"it":[42],"is":[43,150],"important":[44],"identify":[46,104],"locations":[48],"lipid":[50,57,87,106,115,141,186,196,218],"binding":[51,58,107,112,126,197],"relative":[54],"specificities":[55],"at":[59,124,143,157,180],"these":[60,130],"sites.":[61,127,145,159],"In":[62],"our":[63],"previous":[64],"native":[65,131],"mass":[66],"spectrometry":[67],"(MS)":[68],"study,":[69],"we":[70,90],"developed":[71],"a":[72,96,176],"single":[73],"double":[75],"mutant":[76],"analysis":[77],"approach":[78,191],"profile":[80],"contribution":[82],"residues":[85],"toward":[86,152,205],"binding.":[88],"Here,":[89],"extend":[91],"this":[92,189],"method":[93],"by":[94,110],"screening":[95],"broad":[97],"range":[98],"mutants":[100],"AqpZ":[102,149,212],"measuring":[111],"types":[116],"measure":[118],"selectivity":[120,201],"lipids":[123,204],"selected":[125,144],"We":[128,146],"complemented":[129],"MS":[132],"studies":[133],"molecular":[135],"dynamics":[136],"(MD)":[137],"simulations":[138],"visualize":[140],"discovered":[147],"that":[148],"selective":[151],"cardiolipins":[153],"(CL)":[154],"but":[155],"only":[156],"Specifically,":[160],"CL":[161],"orients":[162],"its":[164,171],"headgroup":[165],"facing":[166],"cytoplasmic":[168],"side,":[169],"acyl":[172],"chains":[173],"hydrophobic":[177],"pocket":[178],"located":[179],"monomeric":[182],"interface":[183],"within":[184],"bilayer.":[187],"Overall,":[188],"integrative":[190],"provides":[192],"unique":[193],"insights":[194],"into":[195],"various":[203],"AqpZ,":[206],"enabling":[207],"us":[208],"map":[210],"based":[215],"affinity.":[219]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":3}],"updated_date":"2026-10-15T10:00:46.086208","created_date":"2025-10-10T00:00:00"}