{"id":"https://openalex.org/W4407410223","doi":"https://doi.org/10.1038/s41596-024-01119-3","title":"Massively parallel in vivo Perturb-seq screening","display_name":"Massively parallel in vivo Perturb-seq screening","publication_year":2025,"publication_date":"2025-02-12","ids":{"openalex":"https://openalex.org/W4407410223","doi":"https://doi.org/10.1038/s41596-024-01119-3","pmid":"https://pubmed.ncbi.nlm.nih.gov/39939709","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/12237601"},"language":"en","primary_location":{"id":"doi:10.1038/s41596-024-01119-3","doi":"https://doi.org/10.1038/s41596-024-01119-3","is_oa":false,"landing_page_url":"https://doi.org/10.1038/s41596-024-01119-3","pdf_url":null,"source":{"id":"https://openalex.org/S109387254","display_name":"Nature Protocols","issn_l":"1750-2799","issn":["1750-2799","1754-2189"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-1","ki-jl-2","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature Protocols","raw_type":"journal-article"},"volume":null,"type":"review","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12237601/pdf/nihms-2076570.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5007876361","display_name":"Xinhe Zheng","orcid":"https://orcid.org/0000-0002-4388-6724","observed_orcids":["https://orcid.org/0000-0002-4388-6724"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Xinhe Zheng","raw_affiliation_strings":["Department of Neuroscience, Dorris Neuroscience Center, Scripps Research, La Jolla, CA, USA"],"raw_orcid":"https://orcid.org/0000-0002-4388-6724","affiliations":[{"raw_affiliation_string":"Department of Neuroscience, Dorris Neuroscience Center, Scripps Research, La Jolla, CA, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007744756","display_name":"Patrick C. Thompson","orcid":"https://orcid.org/0009-0006-2755-2819","observed_orcids":["https://orcid.org/0009-0006-2755-2819"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Patrick C. Thompson","raw_affiliation_strings":["Department of Neuroscience, Dorris Neuroscience Center, Scripps Research, La Jolla, CA, USA"],"raw_orcid":"https://orcid.org/0009-0006-2755-2819","affiliations":[{"raw_affiliation_string":"Department of Neuroscience, Dorris Neuroscience Center, Scripps Research, La Jolla, CA, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060125428","display_name":"Cassandra White","orcid":"https://orcid.org/0000-0002-6697-4321","observed_orcids":["https://orcid.org/0000-0002-6697-4321"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Cassandra M. White","raw_affiliation_strings":["Department of Neuroscience, Dorris Neuroscience Center, Scripps Research, La Jolla, CA, USA"],"raw_orcid":"https://orcid.org/0000-0002-6697-4321","affiliations":[{"raw_affiliation_string":"Department of Neuroscience, Dorris Neuroscience Center, Scripps Research, La Jolla, CA, USA","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5081802188","display_name":"Xin Jin","orcid":"https://orcid.org/0000-0003-2344-992X","observed_orcids":["https://orcid.org/0000-0003-2344-992X","https://orcid.org/0000-0002-7271-7655"]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Xin Jin","raw_affiliation_strings":["Department of Neuroscience, Dorris Neuroscience Center, Scripps Research, La Jolla, CA, USA. xinjin@scripps.edu","Department of Neuroscience, Dorris Neuroscience Center, Scripps Research, La Jolla, CA, USA"],"raw_orcid":"https://orcid.org/0000-0003-2344-992X","affiliations":[{"raw_affiliation_string":"Department of Neuroscience, Dorris Neuroscience Center, Scripps Research, La Jolla, CA, USA. xinjin@scripps.edu","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"Department of Neuroscience, Dorris Neuroscience Center, Scripps Research, La Jolla, CA, USA","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5081802188"],"corresponding_institution_ids":["https://openalex.org/I123431417"],"apc_list":null,"apc_paid":null,"fwci":1.317,"has_fulltext":true,"cited_by_count":15,"citation_normalized_percentile":{"value":0.78274206,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":98,"max":99},"biblio":{"volume":"20","issue":"7","first_page":"1733","last_page":"1767"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.4794999957084656,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.4794999957084656,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.4043000042438507,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.0723000019788742,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/genetic-perturbation","display_name":"gene perturbation","score":0.5040000081062317},{"id":"https://openalex.org/keywords/adeno-associated-virus-vectors","display_name":"adeno-associated virus vectors","score":0.9390000104904175},{"id":"https://openalex.org/keywords/single-cell-genomics","display_name":"single-cell genomics","score":0.7480000257492065},{"id":"https://openalex.org/keywords/functional-genomics","display_name":"functional genomics","score":0.734000027179718},{"id":"https://openalex.org/keywords/risk-genes","display_name":"risk genes","score":0.5740000009536743},{"id":"https://openalex.org/keywords/guide-rna-design","display_name":"guide RNA design","score":0.8510000109672546},{"id":"https://openalex.org/keywords/aav-production","display_name":"AAV production","score":0.6439999938011169},{"id":"https://openalex.org/keywords/mouse-model","display_name":"mouse model","score":0.5799999833106995},{"id":"https://openalex.org/keywords/data-analysis-software","display_name":"data analysis software","score":0.6809999942779541}],"concepts":[{"id":"https://openalex.org/C190475519","wikidata":"https://www.wikidata.org/wiki/Q544384","display_name":"Massively parallel","level":2,"score":0.8110324740409851},{"id":"https://openalex.org/C192800701","wikidata":"https://www.wikidata.org/wiki/Q6784807","display_name":"Massive parallel sequencing","level":4,"score":0.6551843285560608},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5460578799247742},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.4301123023033142},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3685912489891052},{"id":"https://openalex.org/C173608175","wikidata":"https://www.wikidata.org/wiki/Q232661","display_name":"Parallel computing","level":1,"score":0.26324912905693054},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.16848063468933105},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.13604283332824707},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.09342801570892334}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000229","descriptor_name":"Dependovirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D023281","descriptor_name":"Genomics","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059010","descriptor_name":"Single-Cell Analysis","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D059014","descriptor_name":"High-Throughput Nucleotide Sequencing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1038/s41596-024-01119-3","doi":"https://doi.org/10.1038/s41596-024-01119-3","is_oa":false,"landing_page_url":"https://doi.org/10.1038/s41596-024-01119-3","pdf_url":null,"source":{"id":"https://openalex.org/S109387254","display_name":"Nature Protocols","issn_l":"1750-2799","issn":["1750-2799","1754-2189"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-1","ki-jl-2","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature Protocols","raw_type":"journal-article"},{"id":"pmid:39939709","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/39939709","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nature protocols","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:12237601","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/12237601","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12237601/pdf/nihms-2076570.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Nat Protoc","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:12237601","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/12237601","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12237601/pdf/nihms-2076570.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Nat Protoc","raw_type":"Text"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.5683000087738037}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3030292564","display_name":null,"funder_award_id":"SFARI #736613","funder_id":"https://openalex.org/F4320306164","funder_display_name":"Simons Foundation"},{"id":"https://openalex.org/G342162296","display_name":null,"funder_award_id":"R01HG012819","funder_id":"https://openalex.org/F4320306085","funder_display_name":"U.S. Department of Health and Human Services"},{"id":"https://openalex.org/G6583531119","display_name":null,"funder_award_id":"R01MH137042","funder_id":"https://openalex.org/F4320306085","funder_display_name":"U.S. Department of Health and Human Services"},{"id":"https://openalex.org/G9000882954","display_name":"In vivo Perturb-map: scalable genetic screens with single-cell and spatial resolution in intact tissues","funder_award_id":"R01HG012819","funder_id":"https://openalex.org/F4320337348","funder_display_name":"National Human Genome Research Institute"},{"id":"https://openalex.org/G9000892527","display_name":"Scalable functional analysis of neuropsychiatric risk genes with spatially integrated in vivo Perturb-seq","funder_award_id":"R01MH137042","funder_id":"https://openalex.org/F4320337346","funder_display_name":"National Institute of Mental Health"}],"funders":[{"id":"https://openalex.org/F4320306085","display_name":"U.S. Department of Health and Human Services","ror":"https://ror.org/033jnv181"},{"id":"https://openalex.org/F4320306144","display_name":"McKnight Endowment Fund for Neuroscience","ror":"https://ror.org/003ghvj67"},{"id":"https://openalex.org/F4320306148","display_name":"Pew Charitable Trusts","ror":"https://ror.org/02xhk2825"},{"id":"https://openalex.org/F4320306164","display_name":"Simons Foundation","ror":"https://ror.org/01cmst727"},{"id":"https://openalex.org/F4320306380","display_name":"Larry L. Hillblom Foundation","ror":"https://ror.org/00br6vq85"},{"id":"https://openalex.org/F4320306403","display_name":"Esther A. and Joseph Klingenstein Fund","ror":"https://ror.org/01q222b25"},{"id":"https://openalex.org/F4320312920","display_name":"G. Harold and Leila Y. Mathers Foundation","ror":"https://ror.org/02a7hjv13"},{"id":"https://openalex.org/F4320315019","display_name":"Simons Foundation Autism Research Initiative","ror":"https://ror.org/01cmst727"},{"id":"https://openalex.org/F4320337346","display_name":"National Institute of Mental Health","ror":"https://ror.org/04xeg9z08"},{"id":"https://openalex.org/F4320337348","display_name":"National Human Genome Research Institute","ror":"https://ror.org/00baak391"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4407410223.pdf","grobid_xml":"https://content.openalex.org/works/W4407410223.grobid-xml"},"referenced_works_count":92,"referenced_works":["https://openalex.org/W1435110089","https://openalex.org/W1655178001","https://openalex.org/W1972476556","https://openalex.org/W1973260880","https://openalex.org/W1977709885","https://openalex.org/W1980310638","https://openalex.org/W1992783297","https://openalex.org/W2006373538","https://openalex.org/W2020098484","https://openalex.org/W2031211461","https://openalex.org/W2052575262","https://openalex.org/W2056512830","https://openalex.org/W2059038945","https://openalex.org/W2123106337","https://openalex.org/W2129975913","https://openalex.org/W2130116522","https://openalex.org/W2166739815","https://openalex.org/W2209402024","https://openalex.org/W2252568502","https://openalex.org/W2256579886","https://openalex.org/W2511569157","https://openalex.org/W2515623196","https://openalex.org/W2523099503","https://openalex.org/W2561508962","https://openalex.org/W2561754210","https://openalex.org/W2562947047","https://openalex.org/W2572662684","https://openalex.org/W2577994754","https://openalex.org/W2601885162","https://openalex.org/W2611446801","https://openalex.org/W2623793810","https://openalex.org/W2708579841","https://openalex.org/W2753180868","https://openalex.org/W2756293223","https://openalex.org/W2766367284","https://openalex.org/W2783322400","https://openalex.org/W2790778573","https://openalex.org/W2794480084","https://openalex.org/W2801454635","https://openalex.org/W2901677030","https://openalex.org/W2904226822","https://openalex.org/W2910480910","https://openalex.org/W2945567049","https://openalex.org/W2950195808","https://openalex.org/W2950715864","https://openalex.org/W2953151710","https://openalex.org/W2964541844","https://openalex.org/W2981110120","https://openalex.org/W2984472267","https://openalex.org/W3002468721","https://openalex.org/W3004887743","https://openalex.org/W3013900499","https://openalex.org/W3015892555","https://openalex.org/W3021105102","https://openalex.org/W3032064403","https://openalex.org/W3045746508","https://openalex.org/W3088246260","https://openalex.org/W3094707224","https://openalex.org/W3110610967","https://openalex.org/W3124760643","https://openalex.org/W3131183594","https://openalex.org/W3141751158","https://openalex.org/W3158694246","https://openalex.org/W3164692211","https://openalex.org/W3190049048","https://openalex.org/W4206229719","https://openalex.org/W4211123163","https://openalex.org/W4211219428","https://openalex.org/W4221016141","https://openalex.org/W4221128536","https://openalex.org/W4224228615","https://openalex.org/W4281714747","https://openalex.org/W4281716291","https://openalex.org/W4293051770","https://openalex.org/W4302010984","https://openalex.org/W4318959472","https://openalex.org/W4322770338","https://openalex.org/W4368347467","https://openalex.org/W4385718412","https://openalex.org/W4386697296","https://openalex.org/W4386886502","https://openalex.org/W4389670555","https://openalex.org/W4393055826","https://openalex.org/W4393153137","https://openalex.org/W4398146760","https://openalex.org/W4400729458","https://openalex.org/W4401736259","https://openalex.org/W4403195735","https://openalex.org/W4403196249","https://openalex.org/W4405003311","https://openalex.org/W4408363679","https://openalex.org/W4413110896"],"related_works":["https://openalex.org/W4398146760","https://openalex.org/W7163189637","https://openalex.org/W2561754210","https://openalex.org/W4386886502","https://openalex.org/W7162469040","https://openalex.org/W4403164968","https://openalex.org/W7042650556","https://openalex.org/W4411238124","https://openalex.org/W7133824434","https://openalex.org/W4416179677"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":9},{"year":2025,"cited_by_count":6}],"updated_date":"2026-11-01T11:40:01.658920","created_date":"2025-02-13T00:00:00"}