{"id":"https://openalex.org/W4406203327","doi":"https://doi.org/10.2323/jgam.2024.12.001","title":"Characterization of the Azotobacter vinelandii nitrogenase complex expressed in Escherichia coli toward further activity improvement","display_name":"Characterization of the Azotobacter vinelandii nitrogenase complex expressed in Escherichia coli toward further activity improvement","publication_year":2024,"publication_date":"2024-01-01","ids":{"openalex":"https://openalex.org/W4406203327","doi":"https://doi.org/10.2323/jgam.2024.12.001","pmid":"https://pubmed.ncbi.nlm.nih.gov/39805616"},"language":"en","primary_location":{"id":"doi:10.2323/jgam.2024.12.001","doi":"https://doi.org/10.2323/jgam.2024.12.001","is_oa":true,"landing_page_url":"https://doi.org/10.2323/jgam.2024.12.001","pdf_url":"https://www.jstage.jst.go.jp/article/jgam/70/5/70_2024.12.001/_pdf","source":{"id":"https://openalex.org/S27908214","display_name":"The Journal of General and Applied Microbiology","issn_l":"0022-1260","issn":["0022-1260","1349-8037"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4323999590","host_organization_name":"Microbiology Research Foundation","host_organization_lineage":["https://openalex.org/P4323999590"],"host_organization_lineage_names":["Microbiology Research Foundation"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of General and Applied Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://www.jstage.jst.go.jp/article/jgam/70/5/70_2024.12.001/_pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5017455178","display_name":"Yusuke Ito","orcid":"https://orcid.org/0000-0002-3383-412X","observed_orcids":["https://orcid.org/0000-0002-3383-412X"]},"institutions":[{"id":"https://openalex.org/I13045471","display_name":"Kikkoman (Japan)","ror":"https://ror.org/00z0d6447","country_code":"JP","type":"company","lineage":["https://openalex.org/I13045471"]},{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yusuke Ito","raw_affiliation_strings":["Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo","Research & Development Division, Kikkoman Corporation"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo","institution_ids":["https://openalex.org/I74801974"]},{"raw_affiliation_string":"Research & Development Division, Kikkoman Corporation","institution_ids":["https://openalex.org/I13045471"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5033302488","display_name":"Daisuke Yoshidome","orcid":"https://orcid.org/0009-0002-4579-1822","observed_orcids":["https://orcid.org/0009-0002-4579-1822"]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Daisuke Yoshidome","raw_affiliation_strings":["Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100826851","display_name":"Makoto Hidaka","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Makoto Hidaka","raw_affiliation_strings":["Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5074857121","display_name":"Yasuko Araki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I13045471","display_name":"Kikkoman (Japan)","ror":"https://ror.org/00z0d6447","country_code":"JP","type":"company","lineage":["https://openalex.org/I13045471"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yasuko Araki","raw_affiliation_strings":["Research & Development Division, Kikkoman Corporation"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research & Development Division, Kikkoman Corporation","institution_ids":["https://openalex.org/I13045471"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015929305","display_name":"Kotaro Ito","orcid":"https://orcid.org/0000-0002-1061-6437","observed_orcids":["https://orcid.org/0000-0002-1061-6437"]},"institutions":[{"id":"https://openalex.org/I13045471","display_name":"Kikkoman (Japan)","ror":"https://ror.org/00z0d6447","country_code":"JP","type":"company","lineage":["https://openalex.org/I13045471"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kotaro Ito","raw_affiliation_strings":["Research & Development Division, Kikkoman Corporation"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research & Development Division, Kikkoman Corporation","institution_ids":["https://openalex.org/I13045471"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021435700","display_name":"Saori Kosono","orcid":"https://orcid.org/0000-0001-7108-5142","observed_orcids":["https://orcid.org/0000-0001-7108-5142"]},"institutions":[{"id":"https://openalex.org/I4405286727","display_name":"Collaborative Research Institute for Innovative Microbiology, The University of Tokyo","ror":"https://ror.org/02xma4s44","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4405286727","https://openalex.org/I74801974"]},{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Saori Kosono","raw_affiliation_strings":["Collaborative Research Institute for Innovative Microbiology, The University of Tokyo","Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Collaborative Research Institute for Innovative Microbiology, The University of Tokyo","institution_ids":["https://openalex.org/I4405286727","https://openalex.org/I74801974"]},{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5088799253","display_name":"Makoto Nishiyama","orcid":"https://orcid.org/0000-0001-8143-8052","observed_orcids":["https://orcid.org/0000-0001-8143-8052"]},"institutions":[{"id":"https://openalex.org/I4405286727","display_name":"Collaborative Research Institute for Innovative Microbiology, The University of Tokyo","ror":"https://ror.org/02xma4s44","country_code":"JP","type":"facility","lineage":["https://openalex.org/I4405286727","https://openalex.org/I74801974"]},{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Makoto Nishiyama","raw_affiliation_strings":["Collaborative Research Institute for Innovative Microbiology, The University of Tokyo","Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Collaborative Research Institute for Innovative Microbiology, The University of Tokyo","institution_ids":["https://openalex.org/I4405286727","https://openalex.org/I74801974"]},{"raw_affiliation_string":"Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo","institution_ids":["https://openalex.org/I74801974"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.0,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"70","issue":"5","first_page":"n/a","last_page":"n/a"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11626","display_name":"Metalloenzymes and iron-sulfur proteins","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11626","display_name":"Metalloenzymes and iron-sulfur proteins","score":0.9990000128746033,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12112","display_name":"Ammonia Synthesis and Nitrogen Reduction","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1503","display_name":"Catalysis"},"field":{"id":"https://openalex.org/fields/15","display_name":"Chemical Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11470","display_name":"Legume Nitrogen Fixing Symbiosis","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/azotobacter-vinelandii","display_name":"Azotobacter vinelandii","score":0.9269999861717224},{"id":"https://openalex.org/keywords/nitrogenase","display_name":"nitrogenase","score":0.9350000023841858},{"id":"https://openalex.org/keywords/heterologous-expression-in-escherichia-coli","display_name":"heterologous expression in Escherichia coli","score":0.8840000033378601},{"id":"https://openalex.org/keywords/nifd","display_name":"nifD","score":0.6840000152587891},{"id":"https://openalex.org/keywords/nifk","display_name":"nifK","score":0.9990000128746033},{"id":"https://openalex.org/keywords/nitrogen-fixation","display_name":"nitrogen fixation","score":0.6830000281333923},{"id":"https://openalex.org/keywords/nifh","display_name":"nifH gene","score":0.6639999747276306},{"id":"https://openalex.org/keywords/enzyme-activity-enhancement","display_name":"enzyme activity enhancement","score":0.503000020980835}],"concepts":[{"id":"https://openalex.org/C2776159390","wikidata":"https://www.wikidata.org/wiki/Q2157875","display_name":"Azotobacter vinelandii","level":5,"score":0.9455709457397461},{"id":"https://openalex.org/C141280058","wikidata":"https://www.wikidata.org/wiki/Q410381","display_name":"Nitrogenase","level":4,"score":0.8126142024993896},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.6622774600982666},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4854498505592346},{"id":"https://openalex.org/C2776740245","wikidata":"https://www.wikidata.org/wiki/Q132995","display_name":"Azotobacter","level":3,"score":0.4429023861885071},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.36214733123779297},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3348073363304138},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.30298611521720886},{"id":"https://openalex.org/C181440489","wikidata":"https://www.wikidata.org/wiki/Q272467","display_name":"Nitrogen fixation","level":3,"score":0.17258283495903015},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.14715677499771118},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.13452023267745972},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.12002331018447876}],"mesh":[{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D002850","descriptor_name":"Chromatography, Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009586","descriptor_name":"Nitrogen Fixation","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D009591","descriptor_name":"Nitrogenase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D009591","descriptor_name":"Nitrogenase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D009591","descriptor_name":"Nitrogenase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009591","descriptor_name":"Nitrogenase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016948","descriptor_name":"Azotobacter vinelandii","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D016948","descriptor_name":"Azotobacter vinelandii","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.2323/jgam.2024.12.001","doi":"https://doi.org/10.2323/jgam.2024.12.001","is_oa":true,"landing_page_url":"https://doi.org/10.2323/jgam.2024.12.001","pdf_url":"https://www.jstage.jst.go.jp/article/jgam/70/5/70_2024.12.001/_pdf","source":{"id":"https://openalex.org/S27908214","display_name":"The Journal of General and Applied Microbiology","issn_l":"0022-1260","issn":["0022-1260","1349-8037"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4323999590","host_organization_name":"Microbiology Research Foundation","host_organization_lineage":["https://openalex.org/P4323999590"],"host_organization_lineage_names":["Microbiology Research Foundation"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of General and Applied Microbiology","raw_type":"journal-article"},{"id":"pmid:39805616","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/39805616","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of general and applied microbiology","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.2323/jgam.2024.12.001","doi":"https://doi.org/10.2323/jgam.2024.12.001","is_oa":true,"landing_page_url":"https://doi.org/10.2323/jgam.2024.12.001","pdf_url":"https://www.jstage.jst.go.jp/article/jgam/70/5/70_2024.12.001/_pdf","source":{"id":"https://openalex.org/S27908214","display_name":"The Journal of General and Applied Microbiology","issn_l":"0022-1260","issn":["0022-1260","1349-8037"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","medline","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4323999590","host_organization_name":"Microbiology Research Foundation","host_organization_lineage":["https://openalex.org/P4323999590"],"host_organization_lineage_names":["Microbiology Research Foundation"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of General and Applied Microbiology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4406203327.pdf","grobid_xml":"https://content.openalex.org/works/W4406203327.grobid-xml"},"referenced_works_count":21,"referenced_works":["https://openalex.org/W263545607","https://openalex.org/W1506479591","https://openalex.org/W1548708435","https://openalex.org/W1631964335","https://openalex.org/W1971439989","https://openalex.org/W2017671198","https://openalex.org/W2040480579","https://openalex.org/W2048600400","https://openalex.org/W2320625005","https://openalex.org/W2591208564","https://openalex.org/W2801029867","https://openalex.org/W2921492061","https://openalex.org/W3002794944","https://openalex.org/W3011033957","https://openalex.org/W3083018475","https://openalex.org/W3192101921","https://openalex.org/W3203026641","https://openalex.org/W4236109816","https://openalex.org/W4323294987","https://openalex.org/W4387871796","https://openalex.org/W4400300889"],"related_works":["https://openalex.org/W4400300889","https://openalex.org/W3192101921","https://openalex.org/W4410026701","https://openalex.org/W1624027648","https://openalex.org/W4221062057","https://openalex.org/W4232981060","https://openalex.org/W4402646589","https://openalex.org/W1542055107","https://openalex.org/W2117325737","https://openalex.org/W2013988017"],"abstract_inverted_index":{"We":[0],"previously":[1],"constructed":[2],"an":[3],"Escherichia":[4],"coli":[5,74],"strain":[6],"expressing":[7],"16":[8],"nitrogen":[9],"fixation":[10],"(nif)":[11],"and":[12,19,50,68],"2":[13],"nif-related":[14],"genes":[15],"from":[16],"Azotobacter":[17],"vinelandii":[18],"improved":[20],"nitrogenase":[21,61,102],"activity":[22],"to":[23,54],"some":[24,83],"extent":[25],"by":[26],"enhancing":[27],"NifH-related":[28],"functions.":[29],"In":[30],"the":[31,36,78,86],"present":[32,76],"study,":[33],"we":[34],"analyzed":[35],"formation":[37],"of":[38,66,89,92],"dinitrogenase,":[39],"a":[40],"heterotetrameric":[41],"NifD2K2,":[42],"produced":[43,71],"in":[44,60,72,85,97],"E.":[45,73],"coli,":[46],"using":[47],"gel-filtration":[48],"chromatography":[49],"blue":[51],"native":[52],"PAGE":[53],"gain":[55],"insight":[56],"into":[57],"further":[58],"increases":[59],"activity.":[62,103],"A":[63],"certain":[64],"proportion":[65],"NifD":[67],"NifK":[69],"proteins":[70],"were":[75],"as":[77],"complete":[79],"NifD2K2":[80],"component,":[81],"but":[82],"remained":[84],"intermediate":[87],"stages":[88],"maturation.":[90],"Overexpression":[91],"nafY,":[93],"which":[94],"is":[95],"involved":[96],"holo-NifD2K2":[98],"formation,":[99],"effectively":[100],"increased":[101]},"counts_by_year":[],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2025-01-09T00:00:00"}
