{"id":"https://openalex.org/W4405963312","doi":"https://doi.org/10.1002/bab.2711","title":"Enhanced Synthesis, Purification, and Characterization of a Marine Bacterial Consortium\u2010Derived Protease Enzyme With Destaining and Keratinolytic Activity","display_name":"Enhanced Synthesis, Purification, and Characterization of a Marine Bacterial Consortium\u2010Derived Protease Enzyme With Destaining and Keratinolytic Activity","publication_year":2024,"publication_date":"2024-12-30","ids":{"openalex":"https://openalex.org/W4405963312","doi":"https://doi.org/10.1002/bab.2711","pmid":"https://pubmed.ncbi.nlm.nih.gov/39740189"},"language":"en","primary_location":{"id":"doi:10.1002/bab.2711","doi":"https://doi.org/10.1002/bab.2711","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bab.2711","pdf_url":null,"source":{"id":"https://openalex.org/S34872747","display_name":"Biotechnology and Applied Biochemistry","issn_l":"0885-4513","issn":["0885-4513","1470-8744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Applied Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5016474719","display_name":"Rashmi Thakor","orcid":"https://orcid.org/0000-0003-2272-1362","observed_orcids":["https://orcid.org/0000-0003-2272-1362"]},"institutions":[{"id":"https://openalex.org/I335036346","display_name":"Hemchandracharya North Gujarat University","ror":"https://ror.org/049sxg896","country_code":"IN","type":"education","lineage":["https://openalex.org/I335036346"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Rashmi Thakor","raw_affiliation_strings":["Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, Gujarat, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, Gujarat, India","institution_ids":["https://openalex.org/I335036346"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004463496","display_name":"Harsh Mistry","orcid":"https://orcid.org/0000-0001-8650-0548","observed_orcids":["https://orcid.org/0000-0001-8650-0548"]},"institutions":[{"id":"https://openalex.org/I335036346","display_name":"Hemchandracharya North Gujarat University","ror":"https://ror.org/049sxg896","country_code":"IN","type":"education","lineage":["https://openalex.org/I335036346"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Harsh Mistry","raw_affiliation_strings":["Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, Gujarat, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, Gujarat, India","institution_ids":["https://openalex.org/I335036346"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084805819","display_name":"Hesham Saleh M. Almoallim","orcid":"https://orcid.org/0009-0008-9011-5720","observed_orcids":["https://orcid.org/0009-0008-9011-5720"]},"institutions":[{"id":"https://openalex.org/I28022161","display_name":"King Saud University","ror":"https://ror.org/02f81g417","country_code":"SA","type":"education","lineage":["https://openalex.org/I28022161"]}],"countries":["SA"],"is_corresponding":false,"raw_author_name":"Hesham S. Almoallim","raw_affiliation_strings":["Department of Oral and Maxillofacial Surgery, College of Dentistry, King Saud University, Riyadh, Saudi Arabia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Oral and Maxillofacial Surgery, College of Dentistry, King Saud University, Riyadh, Saudi Arabia","institution_ids":["https://openalex.org/I28022161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101735879","display_name":"Mohammad Javed Ansari","orcid":"https://orcid.org/0000-0003-1520-6055","observed_orcids":["https://orcid.org/0000-0003-1520-6055"]},"institutions":[{"id":"https://openalex.org/I139858224","display_name":"M.J.P. Rohilkhand University","ror":"https://ror.org/02e3nay30","country_code":"IN","type":"education","lineage":["https://openalex.org/I139858224"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Mohammad Javed Ansari","raw_affiliation_strings":["Department of Botany, Hindu College Moradabad, Mahatma Jyotiba Phule Rohilkhand University, Bareilly, Uttar Pradesh, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Botany, Hindu College Moradabad, Mahatma Jyotiba Phule Rohilkhand University, Bareilly, Uttar Pradesh, India","institution_ids":["https://openalex.org/I139858224"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084015464","display_name":"Ashish Patel","orcid":"https://orcid.org/0000-0003-4612-1724","observed_orcids":["https://orcid.org/0000-0003-4612-1724"]},"institutions":[{"id":"https://openalex.org/I335036346","display_name":"Hemchandracharya North Gujarat University","ror":"https://ror.org/049sxg896","country_code":"IN","type":"education","lineage":["https://openalex.org/I335036346"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Ashish Patel","raw_affiliation_strings":["Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, Gujarat, India"],"raw_orcid":"https://orcid.org/0000-0003-4612-1724","affiliations":[{"raw_affiliation_string":"Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, Gujarat, India","institution_ids":["https://openalex.org/I335036346"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044784780","display_name":"Virendra Kumar Yadav","orcid":"https://orcid.org/0000-0001-5448-7676","observed_orcids":["https://orcid.org/0000-0001-5448-7676","https://orcid.org/0009-0004-4226-9996"]},"institutions":[{"id":"https://openalex.org/I3132999081","display_name":"Marwadi University","ror":"https://ror.org/030dn1812","country_code":"IN","type":"education","lineage":["https://openalex.org/I3132999081"]},{"id":"https://openalex.org/I335036346","display_name":"Hemchandracharya North Gujarat University","ror":"https://ror.org/049sxg896","country_code":"IN","type":"education","lineage":["https://openalex.org/I335036346"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Virendra Kumar Yadav","raw_affiliation_strings":["Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, Gujarat, India","Department of Microbiology, Faculty of Sciences, Marwadi University Research Center, Rajkot, Gujarat, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, Gujarat, India","institution_ids":["https://openalex.org/I335036346"]},{"raw_affiliation_string":"Department of Microbiology, Faculty of Sciences, Marwadi University Research Center, Rajkot, Gujarat, India","institution_ids":["https://openalex.org/I3132999081"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001395805","display_name":"Dipak Kumar Sahoo","orcid":"https://orcid.org/0000-0003-3748-3395","observed_orcids":["https://orcid.org/0000-0003-3748-3395"]},"institutions":[{"id":"https://openalex.org/I173911158","display_name":"Iowa State University","ror":"https://ror.org/04rswrd78","country_code":"US","type":"education","lineage":["https://openalex.org/I173911158"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dipak Kumar Sahoo","raw_affiliation_strings":["Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA","institution_ids":["https://openalex.org/I173911158"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5020317264","display_name":"Himanshu Bariya","orcid":"https://orcid.org/0000-0003-3059-6338","observed_orcids":["https://orcid.org/0000-0003-3059-6338","https://orcid.org/0000-0001-5796-8918"]},"institutions":[{"id":"https://openalex.org/I335036346","display_name":"Hemchandracharya North Gujarat University","ror":"https://ror.org/049sxg896","country_code":"IN","type":"education","lineage":["https://openalex.org/I335036346"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Himanshu Bariya","raw_affiliation_strings":["Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, Gujarat, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, Gujarat, India","institution_ids":["https://openalex.org/I335036346"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5020317264","https://openalex.org/A5084015464"],"corresponding_institution_ids":["https://openalex.org/I335036346"],"apc_list":{"value":3300,"currency":"USD","value_usd":3300},"apc_paid":null,"fwci":0.8314,"has_fulltext":false,"cited_by_count":5,"citation_normalized_percentile":{"value":0.61439438,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":97},"biblio":{"volume":"72","issue":"4","first_page":"963","last_page":"973"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13817","display_name":"Tannin, Tannase and Anticancer Activities","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protease-production","display_name":"protease production","score":0.7670000195503235},{"id":"https://openalex.org/keywords/marine-bacteria","display_name":"marine bacteria","score":0.734000027179718},{"id":"https://openalex.org/keywords/bacillus-paralicheniformis","display_name":"Bacillus paralicheniformis","score":0.9679999947547913},{"id":"https://openalex.org/keywords/bacillus-haynesii","display_name":"Bacillus haynesii","score":1.0},{"id":"https://openalex.org/keywords/bacterial-consortia","display_name":"bacterial consortium","score":0.984000027179718},{"id":"https://openalex.org/keywords/keratinolytic-activity","display_name":"keratinolytic activity","score":0.8029999732971191},{"id":"https://openalex.org/keywords/stain-removal","display_name":"stain removal","score":0.6940000057220459},{"id":"https://openalex.org/keywords/textile-industry","display_name":"textile industry","score":0.47699999809265137},{"id":"https://openalex.org/keywords/tanneries","display_name":"tanneries","score":0.7599999904632568},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.7720000147819519},{"id":"https://openalex.org/keywords/alkaline-tolerance","display_name":"alkaline tolerance","score":0.574999988079071},{"id":"https://openalex.org/keywords/metal-ion-effects-on-enzyme-activity","display_name":"metal ion effects on enzyme activity","score":0.7289999723434448},{"id":"https://openalex.org/keywords/fermentation-medium-optimization","display_name":"fermentation medium optimization","score":0.5839999914169312}],"concepts":[{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6317236423492432},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.6170271039009094},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5316506028175354},{"id":"https://openalex.org/C2992246531","wikidata":"https://www.wikidata.org/wiki/Q13107625","display_name":"Alkaline protease","level":4,"score":0.49735167622566223},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.46082603931427},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4590550661087036},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4467412531375885},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.4313586950302124},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001407","descriptor_name":"Bacillus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D001407","descriptor_name":"Bacillus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D001407","descriptor_name":"Bacillus","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":true},{"descriptor_ui":"D001407","descriptor_name":"Bacillus","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007218","descriptor_name":"Industrial Microbiology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D010447","descriptor_name":"Peptide Hydrolases","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D010447","descriptor_name":"Peptide Hydrolases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D010447","descriptor_name":"Peptide Hydrolases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D012336","descriptor_name":"RNA, Ribosomal, 16S","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012623","descriptor_name":"Seawater","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D013783","descriptor_name":"Textile Industry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D019015","descriptor_name":"Geologic Sediments","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D059001","descriptor_name":"Aquatic Organisms","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D059001","descriptor_name":"Aquatic Organisms","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D059001","descriptor_name":"Aquatic Organisms","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D059013","descriptor_name":"Microbial Consortia","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1002/bab.2711","doi":"https://doi.org/10.1002/bab.2711","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bab.2711","pdf_url":null,"source":{"id":"https://openalex.org/S34872747","display_name":"Biotechnology and Applied Biochemistry","issn_l":"0885-4513","issn":["0885-4513","1470-8744"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Applied Biochemistry","raw_type":"journal-article"},{"id":"pmid:39740189","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/39740189","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and applied biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Responsible consumption and production","id":"https://metadata.un.org/sdg/12","score":0.5925999879837036}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320321145","display_name":"King Saud University","ror":"https://ror.org/02f81g417"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":57,"referenced_works":["https://openalex.org/W126109032","https://openalex.org/W1841605125","https://openalex.org/W1967960656","https://openalex.org/W1968788542","https://openalex.org/W1988664298","https://openalex.org/W1997899213","https://openalex.org/W2001177772","https://openalex.org/W2008912694","https://openalex.org/W2021548280","https://openalex.org/W2035522599","https://openalex.org/W2038345298","https://openalex.org/W2054783695","https://openalex.org/W2067545501","https://openalex.org/W2113442102","https://openalex.org/W2118301259","https://openalex.org/W2293835680","https://openalex.org/W2516925297","https://openalex.org/W2592562560","https://openalex.org/W2744187965","https://openalex.org/W2758035164","https://openalex.org/W2762574073","https://openalex.org/W2765301717","https://openalex.org/W2773790897","https://openalex.org/W2784077372","https://openalex.org/W2792944996","https://openalex.org/W2804652854","https://openalex.org/W2807015771","https://openalex.org/W2810767609","https://openalex.org/W2889153973","https://openalex.org/W2891174792","https://openalex.org/W2896116612","https://openalex.org/W2947899772","https://openalex.org/W2949816023","https://openalex.org/W2949817856","https://openalex.org/W2979795427","https://openalex.org/W2985022890","https://openalex.org/W2995115456","https://openalex.org/W3004831660","https://openalex.org/W3011988590","https://openalex.org/W3032266086","https://openalex.org/W3035955347","https://openalex.org/W3041753265","https://openalex.org/W3047243981","https://openalex.org/W3086758234","https://openalex.org/W3112234213","https://openalex.org/W3125167241","https://openalex.org/W3127166681","https://openalex.org/W3137000047","https://openalex.org/W3158828657","https://openalex.org/W3165567665","https://openalex.org/W3182991463","https://openalex.org/W3198740146","https://openalex.org/W3206942860","https://openalex.org/W4206763613","https://openalex.org/W4225428160","https://openalex.org/W4237931901","https://openalex.org/W4292481139"],"related_works":["https://openalex.org/W4402996189","https://openalex.org/W2404390622","https://openalex.org/W2038913723","https://openalex.org/W7154283279","https://openalex.org/W2062688162","https://openalex.org/W2885321710","https://openalex.org/W4386851354","https://openalex.org/W7118174635","https://openalex.org/W2134097223","https://openalex.org/W1988664298"],"abstract_inverted_index":{"ABSTRACT":[0],"Two":[1],"marine\u2010derived":[2],"bacteria,":[3],"Bacillus":[4,8],"paralicheniformis":[5],"(HR\u20101)":[6],"and":[7,15,23,60,70,103,121,141,153,163,194],"haynesii":[9],"(HR\u20105),":[10],"were":[11,74],"isolated":[12],"from":[13,39,159,171],"sediments":[14],"identified":[16],"using":[17,76],"16S":[18],"ribosomal":[19],"RNA":[20],"gene":[21],"amplification":[22],"sequencing":[24],"as":[25,27,149,173,175],"well":[26,174],"biochemical":[28],"analysis.":[29],"The":[30,131,155],"development":[31],"of":[32,49,128,146],"a":[33],"bacterial":[34],"consortium":[35,82,161],"(HR\u20101":[36],"&":[37,84],"HR\u20105)":[38],"these":[40],"two":[41],"bacteria":[42],"was":[43],"used":[44],"to":[45],"increase":[46],"the":[47,50,71,95,144,160,192],"production":[48,89],"protease":[51,88,157],"enzyme":[52,133,185],"under":[53],"various":[54],"conditions,":[55],"including":[56],"fermentation":[57],"media,":[58],"carbon":[59],"nitrogen":[61],"sources":[62],"(1%":[63,112,123],"w/v),":[64,113],"different":[65],"pH":[66,119,139],"levels,":[67],"incubation":[68,129],"time,":[69],"obtained":[72,158],"enzyme,":[73],"detected":[75],"SDS\u2010PAGE":[77],"followed":[78],"by":[79],"purification.":[80],"Bacterial":[81],"HR\u20101":[83,98,162],"HR\u20105":[85,104,164],"exhibited":[86],"maximum":[87],"(330.42":[90],"\u00b1":[91,100,106],"4.47":[92],"U/mL)":[93,102,108],"than":[94],"individual":[96],"isolates":[97],"(156.32":[99],"2.14":[101],"(185.73":[105],"5.14":[107],"on":[109],"supplementing":[110],"peptone":[111],"2.8%":[114],"skim":[115],"milk":[116],"+":[117],"N\u2010broth,":[118],"9,":[120],"dextrose":[122],"w/v)":[124],"after":[125],"48":[126],"h":[127],"time.":[130],"purified":[132,156],"showed":[134],"increased":[135],"activity":[136],"at":[137],"alkaline":[138],"9.0":[140],"also":[142],"in":[143,191],"presence":[145],"ions":[147],"such":[148],"Ca+2,":[150],"Fe+3,":[151],"Mg+2,":[152],"Mn+2.":[154],"shows":[165],"improved":[166],"efficiency":[167,178],"for":[168,179,187],"stain":[169],"removal":[170],"cloth":[172],"high":[176],"keratinolytic":[177],"poultry":[180],"feather":[181],"degradation,":[182],"making":[183],"this":[184],"suitable":[186],"industrial":[188],"use,":[189],"particularly":[190],"textile":[193],"tannery":[195],"sectors.":[196]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":2}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2025-01-01T00:00:00"}