{"id":"https://openalex.org/W4405456015","doi":"https://doi.org/10.1080/14620316.2024.2438660","title":"Optimising the PVS2 treatment duration for cryopreserving seeds of the endangered rheophytic orchid Epipactis flava Seidenf","display_name":"Optimising the PVS2 treatment duration for cryopreserving seeds of the endangered rheophytic orchid Epipactis flava Seidenf","publication_year":2024,"publication_date":"2024-12-15","ids":{"openalex":"https://openalex.org/W4405456015","doi":"https://doi.org/10.1080/14620316.2024.2438660"},"language":"en","primary_location":{"id":"doi:10.1080/14620316.2024.2438660","doi":"https://doi.org/10.1080/14620316.2024.2438660","is_oa":false,"landing_page_url":"https://doi.org/10.1080/14620316.2024.2438660","pdf_url":null,"source":{"id":"https://openalex.org/S55420515","display_name":"The Journal of Horticultural Science and Biotechnology","issn_l":"1462-0316","issn":["1462-0316","2380-4084"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","norway-1","poland-40","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Horticultural Science and Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060221262","display_name":"Julaluk Linjikao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I66200439","display_name":"Naresuan University","ror":"https://ror.org/03e2qe334","country_code":"TH","type":"education","lineage":["https://openalex.org/I66200439"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Julaluk Linjikao","raw_affiliation_strings":["Naresuan University","Plant Tissue Culture Research Unit, Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Naresuan University","institution_ids":["https://openalex.org/I66200439"]},{"raw_affiliation_string":"Plant Tissue Culture Research Unit, Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, Thailand","institution_ids":["https://openalex.org/I66200439"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012642875","display_name":"Phithak Inthima","orcid":"https://orcid.org/0000-0002-5587-6259","observed_orcids":["https://orcid.org/0000-0002-5587-6259"]},"institutions":[{"id":"https://openalex.org/I66200439","display_name":"Naresuan University","ror":"https://ror.org/03e2qe334","country_code":"TH","type":"education","lineage":["https://openalex.org/I66200439"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Phithak Inthima","raw_affiliation_strings":["Naresuan University","Center of Excellence in Research for Agricultural Biotechnology, Naresuan University, Phitsanulok, Thailand","Plant Tissue Culture Research Unit, Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Naresuan University","institution_ids":["https://openalex.org/I66200439"]},{"raw_affiliation_string":"Center of Excellence in Research for Agricultural Biotechnology, Naresuan University, Phitsanulok, Thailand","institution_ids":["https://openalex.org/I66200439"]},{"raw_affiliation_string":"Plant Tissue Culture Research Unit, Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, Thailand","institution_ids":["https://openalex.org/I66200439"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018018854","display_name":"Apinun Limmongkon","orcid":"https://orcid.org/0000-0003-4689-7303","observed_orcids":["https://orcid.org/0000-0003-4689-7303"]},"institutions":[{"id":"https://openalex.org/I66200439","display_name":"Naresuan University","ror":"https://ror.org/03e2qe334","country_code":"TH","type":"education","lineage":["https://openalex.org/I66200439"]}],"countries":["TH"],"is_corresponding":false,"raw_author_name":"Apinun Limmongkon","raw_affiliation_strings":["Naresuan University","Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Naresuan University","institution_ids":["https://openalex.org/I66200439"]},{"raw_affiliation_string":"Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand","institution_ids":["https://openalex.org/I66200439"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5040329151","display_name":"Anupan Kongbangkerd","orcid":"https://orcid.org/0000-0001-5515-2552","observed_orcids":["https://orcid.org/0000-0001-5515-2552"]},"institutions":[{"id":"https://openalex.org/I66200439","display_name":"Naresuan University","ror":"https://ror.org/03e2qe334","country_code":"TH","type":"education","lineage":["https://openalex.org/I66200439"]}],"countries":["TH"],"is_corresponding":true,"raw_author_name":"Anupan Kongbangkerd","raw_affiliation_strings":["Naresuan University","Center of Excellence in Research for Agricultural Biotechnology, Naresuan University, Phitsanulok, Thailand","Plant Tissue Culture Research Unit, Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, Thailand"],"raw_orcid":"https://orcid.org/0000-0001-5515-2552","affiliations":[{"raw_affiliation_string":"Naresuan University","institution_ids":["https://openalex.org/I66200439"]},{"raw_affiliation_string":"Center of Excellence in Research for Agricultural Biotechnology, Naresuan University, Phitsanulok, Thailand","institution_ids":["https://openalex.org/I66200439"]},{"raw_affiliation_string":"Plant Tissue Culture Research Unit, Department of Biology, Faculty of Science, Naresuan University, Phitsanulok, Thailand","institution_ids":["https://openalex.org/I66200439"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5040329151"],"corresponding_institution_ids":["https://openalex.org/I66200439"],"apc_list":null,"apc_paid":null,"fwci":0.2727,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.49288042,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":96,"max":97},"biblio":{"volume":"100","issue":"4","first_page":"546","last_page":"560"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9707000255584717,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10240","display_name":"Plant tissue culture and regeneration","score":0.9707000255584717,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11098","display_name":"Seed Germination and Physiology","score":0.015799999237060547,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12834","display_name":"Flowering Plant Growth and Cultivation","score":0.003000000026077032,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/vitrification","display_name":"vitrification","score":0.8619999885559082},{"id":"https://openalex.org/keywords/pvs2","display_name":"PVS2","score":0.9520000219345093},{"id":"https://openalex.org/keywords/seed-dehydration","display_name":"seed dehydration","score":0.6340000033378601},{"id":"https://openalex.org/keywords/seed-germination","display_name":"seed germination","score":0.7049999833106995},{"id":"https://openalex.org/keywords/protocorm-development","display_name":"protocorm development","score":0.972000002861023},{"id":"https://openalex.org/keywords/endangered-orchids","display_name":"endangered orchids","score":0.6100000143051147},{"id":"https://openalex.org/keywords/in-vitro-conservation","display_name":"in vitro conservation","score":0.40799999237060547}],"concepts":[{"id":"https://openalex.org/C2781370656","wikidata":"https://www.wikidata.org/wiki/Q25308","display_name":"Orchidaceae","level":2,"score":0.5666771531105042},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5579454898834229},{"id":"https://openalex.org/C179345059","wikidata":"https://www.wikidata.org/wiki/Q11394","display_name":"Endangered species","level":3,"score":0.46024128794670105},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.45677322149276733},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.4065755009651184},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.08535391092300415},{"id":"https://openalex.org/C185933670","wikidata":"https://www.wikidata.org/wiki/Q52105","display_name":"Habitat","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1080/14620316.2024.2438660","doi":"https://doi.org/10.1080/14620316.2024.2438660","is_oa":false,"landing_page_url":"https://doi.org/10.1080/14620316.2024.2438660","pdf_url":null,"source":{"id":"https://openalex.org/S55420515","display_name":"The Journal of Horticultural Science and Biotechnology","issn_l":"1462-0316","issn":["1462-0316","2380-4084"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","norway-1","poland-40","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Horticultural Science and Biotechnology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":101,"referenced_works":["https://openalex.org/W8018719","https://openalex.org/W130232318","https://openalex.org/W339577004","https://openalex.org/W647629623","https://openalex.org/W1536460657","https://openalex.org/W1913129032","https://openalex.org/W1916233541","https://openalex.org/W1964407860","https://openalex.org/W1965140948","https://openalex.org/W1965413255","https://openalex.org/W1967054079","https://openalex.org/W1987192082","https://openalex.org/W1989810121","https://openalex.org/W1990150270","https://openalex.org/W1998092686","https://openalex.org/W2024437957","https://openalex.org/W2024556021","https://openalex.org/W2025639892","https://openalex.org/W2031419077","https://openalex.org/W2034428526","https://openalex.org/W2039062397","https://openalex.org/W2043803614","https://openalex.org/W2054746091","https://openalex.org/W2056094915","https://openalex.org/W2071055146","https://openalex.org/W2071478651","https://openalex.org/W2086836401","https://openalex.org/W2098550429","https://openalex.org/W2103746104","https://openalex.org/W2131032142","https://openalex.org/W2136520697","https://openalex.org/W2141188045","https://openalex.org/W2143139062","https://openalex.org/W2143604170","https://openalex.org/W2152328428","https://openalex.org/W2187239605","https://openalex.org/W2188040342","https://openalex.org/W2202354986","https://openalex.org/W2239844189","https://openalex.org/W2409017673","https://openalex.org/W2409195626","https://openalex.org/W2410224116","https://openalex.org/W2410883637","https://openalex.org/W2414549478","https://openalex.org/W2480943605","https://openalex.org/W2545010836","https://openalex.org/W2590283695","https://openalex.org/W2607136061","https://openalex.org/W2618915150","https://openalex.org/W2736540735","https://openalex.org/W2788127583","https://openalex.org/W2790154843","https://openalex.org/W2790710267","https://openalex.org/W2804658160","https://openalex.org/W2885191778","https://openalex.org/W2887991692","https://openalex.org/W2891159855","https://openalex.org/W2891824103","https://openalex.org/W2899793679","https://openalex.org/W2902155462","https://openalex.org/W2903561682","https://openalex.org/W2910216420","https://openalex.org/W2910319240","https://openalex.org/W2924436465","https://openalex.org/W2930156667","https://openalex.org/W2948644880","https://openalex.org/W2950704595","https://openalex.org/W2955961808","https://openalex.org/W2972174451","https://openalex.org/W2985276515","https://openalex.org/W2990495361","https://openalex.org/W3010813748","https://openalex.org/W3013551242","https://openalex.org/W3021943266","https://openalex.org/W3024717368","https://openalex.org/W3087400327","https://openalex.org/W3131293757","https://openalex.org/W3133495896","https://openalex.org/W3144909791","https://openalex.org/W3164765217","https://openalex.org/W3169768344","https://openalex.org/W3181361529","https://openalex.org/W3212526183","https://openalex.org/W3217529580","https://openalex.org/W4205518475","https://openalex.org/W4210512661","https://openalex.org/W4210805996","https://openalex.org/W4211072686","https://openalex.org/W4220791819","https://openalex.org/W4220982698","https://openalex.org/W4241702165","https://openalex.org/W4309703490","https://openalex.org/W4315567972","https://openalex.org/W4319241198","https://openalex.org/W4376873638","https://openalex.org/W4382489298","https://openalex.org/W4386605742","https://openalex.org/W4390904017","https://openalex.org/W4396904624","https://openalex.org/W4399347044","https://openalex.org/W6960901190"],"related_works":["https://openalex.org/W4388813362","https://openalex.org/W7197067817","https://openalex.org/W2364204455","https://openalex.org/W1256976648","https://openalex.org/W72786897","https://openalex.org/W3155149135","https://openalex.org/W1998092686","https://openalex.org/W4386504700","https://openalex.org/W2891824103","https://openalex.org/W1988149918"],"abstract_inverted_index":{"Epipactis":[0],"flava":[1,31,186],"Seidenf.,":[2],"an":[3,20],"endangered":[4],"Thai":[5],"rheophytic":[6],"orchid,":[7],"urgently":[8],"requires":[9],"protection":[10],"to":[11,18,86,103,164,167],"prevent":[12],"its":[13],"extinction.":[14],"This":[15],"study":[16],"aimed":[17],"develop":[19],"effective":[21],"vitrification":[22,45,190],"cryopreservation":[23,183],"protocol":[24],"for":[25,49,66,77,90,98,182],"the":[26,70,82,107,117,125,156,168,177,189],"long-term":[27],"storage":[28],"of":[29,114,124,138,150,184],"E.":[30,185],"seeds.":[32],"Mature":[33],"seeds":[34,71,158,187],"were":[35,72,84],"pretreated":[36],"with":[37,43,96],"a":[38],"loading":[39],"solution":[40,46],"and":[41,54,80,101,122,133],"dehydrated":[42],"plant":[44],"2":[47],"(PVS2)":[48],"0,":[50],"30,":[51],"60,":[52],"90,":[53],"120":[55,112,148,193],"min":[56,100,113,149],"at":[57,140,147],"25":[58],"\u00b1":[59],"2\u00b0C":[60],"before":[61],"immersion":[62],"in":[63,155],"liquid":[64,104,169],"nitrogen":[65,105,170],"24":[67],"hours.":[68],"Post-cryopreservation,":[69],"germinated":[73],"on":[74],"BM-1":[75],"medium":[76,89],"8":[78,92],"weeks,":[79],"then":[81],"protocorms":[83,126,139],"transferred":[85],"semi-solid":[87],"MS":[88],"another":[91],"weeks.":[93],"Seeds":[94],"treated":[95],"PVS2":[97,165,179],"90":[99],"exposed":[102,163],"showed":[106,175],"highest":[108,118,136],"viability":[109],"(33.3%),":[110],"while":[111],"dehydration":[115],"yielded":[116],"germination":[119,153],"rates":[120],"(17.8%)":[121],"5.8%":[123],"reached":[127],"advanced":[128],"developmental":[129],"stages":[130],"(stage":[131],"4":[132],"5).":[134],"The":[135],"percentage":[137],"stage":[141],"5":[142],"(3.3%)":[143],"was":[144,192],"also":[145],"observed":[146],"PVS2.":[151],"No":[152],"occurred":[154],"cryopreserved":[157],"that":[159,176],"had":[160],"not":[161],"been":[162],"prior":[166],"treatment.":[171],"Thus,":[172],"our":[173],"results":[174],"optimal":[178],"exposure":[180],"time":[181],"using":[188],"method":[191],"min.":[194]},"counts_by_year":[{"year":2026,"cited_by_count":2}],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2024-12-17T00:00:00"}