{"id":"https://openalex.org/W4405113483","doi":"https://doi.org/10.1002/edn3.70046","title":"Monitoring of Endangered Freshwater Mussels in Sweden Using Digital PCR","display_name":"Monitoring of Endangered Freshwater Mussels in Sweden Using Digital PCR","publication_year":2024,"publication_date":"2024-11-01","ids":{"openalex":"https://openalex.org/W4405113483","doi":"https://doi.org/10.1002/edn3.70046"},"language":"en","primary_location":{"id":"doi:10.1002/edn3.70046","doi":"https://doi.org/10.1002/edn3.70046","is_oa":true,"landing_page_url":"https://doi.org/10.1002/edn3.70046","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/edn3.70046","source":{"id":"https://openalex.org/S4210176772","display_name":"Environmental DNA","issn_l":"2637-4943","issn":["2637-4943"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-1","norway-1","poland-20","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Environmental DNA","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W4405113483"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/edn3.70046","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5108479976","display_name":"Per Sundberg","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I881427289","display_name":"University of Gothenburg","ror":"https://ror.org/01tm6cn81","country_code":"SE","type":"education","lineage":["https://openalex.org/I881427289"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Per Sundberg","raw_affiliation_strings":["Department of Marine Sciences University of Gothenburg  Goteborg Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Marine Sciences University of Gothenburg  Goteborg Sweden","institution_ids":["https://openalex.org/I881427289"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5025931134","display_name":"Alizz Axberg","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I881427289","display_name":"University of Gothenburg","ror":"https://ror.org/01tm6cn81","country_code":"SE","type":"education","lineage":["https://openalex.org/I881427289"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Alizz Axberg","raw_affiliation_strings":["Department of Marine Sciences University of Gothenburg  Goteborg Sweden","SeAnalytics AB  Bohus\u2010Bj\u00f6rk\u00f6 Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Marine Sciences University of Gothenburg  Goteborg Sweden","institution_ids":["https://openalex.org/I881427289"]},{"raw_affiliation_string":"SeAnalytics AB  Bohus\u2010Bj\u00f6rk\u00f6 Sweden","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077231351","display_name":"Nauras Daraghmeh","orcid":"https://orcid.org/0000-0001-8261-824X","observed_orcids":["https://orcid.org/0000-0001-8261-824X"]},"institutions":[{"id":"https://openalex.org/I881427289","display_name":"University of Gothenburg","ror":"https://ror.org/01tm6cn81","country_code":"SE","type":"education","lineage":["https://openalex.org/I881427289"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Nauras Daragmeh","raw_affiliation_strings":["Department of Marine Sciences University of Gothenburg  Goteborg Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Marine Sciences University of Gothenburg  Goteborg Sweden","institution_ids":["https://openalex.org/I881427289"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016594989","display_name":"Niklas Wengstr\u00f6m","orcid":"https://orcid.org/0000-0001-7575-5932","observed_orcids":["https://orcid.org/0000-0001-7575-5932"]},"institutions":[{"id":"https://openalex.org/I1331223999","display_name":"Swedish National Board of Fisheries","ror":"https://ror.org/000ncdv93","country_code":"SE","type":"government","lineage":["https://openalex.org/I1331223999","https://openalex.org/I4210094408"]},{"id":"https://openalex.org/I4210094408","display_name":"Swedish Agency for Marine and Water Management","ror":"https://ror.org/00ht4dp10","country_code":"SE","type":"government","lineage":["https://openalex.org/I4210094408"]},{"id":"https://openalex.org/I4387155768","display_name":"Swedish National Data Service","ror":"https://ror.org/00ancw882","country_code":"SE","type":"other","lineage":["https://openalex.org/I4387155768"]}],"countries":["SE"],"is_corresponding":false,"raw_author_name":"Niklas Wengstr\u00f6m","raw_affiliation_strings":["Swedish Anglers Association  Gothenburg Sweden"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Swedish Anglers Association  Gothenburg Sweden","institution_ids":["https://openalex.org/I1331223999","https://openalex.org/I4210094408","https://openalex.org/I4387155768"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5045734416","display_name":"Marina Panova","orcid":"https://orcid.org/0000-0002-4147-6473","observed_orcids":["https://orcid.org/0000-0002-4147-6473"]},"institutions":[{"id":"https://openalex.org/I881427289","display_name":"University of Gothenburg","ror":"https://ror.org/01tm6cn81","country_code":"SE","type":"education","lineage":["https://openalex.org/I881427289"]}],"countries":["SE"],"is_corresponding":true,"raw_author_name":"Marina Panova","raw_affiliation_strings":["Department of Marine Sciences University of Gothenburg  Goteborg Sweden"],"raw_orcid":"https://orcid.org/0000-0002-4147-6473","affiliations":[{"raw_affiliation_string":"Department of Marine Sciences University of Gothenburg  Goteborg Sweden","institution_ids":["https://openalex.org/I881427289"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5045734416"],"corresponding_institution_ids":["https://openalex.org/I881427289"],"apc_list":{"value":2880,"currency":"USD","value_usd":2880},"apc_paid":{"value":2880,"currency":"USD","value_usd":2880},"fwci":0.6909,"has_fulltext":true,"cited_by_count":3,"citation_normalized_percentile":{"value":0.64801365,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"6","issue":"6","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12097","display_name":"Aquatic Invertebrate Ecology and Behavior","score":0.002099999925121665,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10012","display_name":"Genetic diversity and population structure","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/freshwater-mussels","display_name":"freshwater mussels","score":0.9769999980926514},{"id":"https://openalex.org/keywords/endangered-species-monitoring","display_name":"endangered species monitoring","score":0.8690000176429749},{"id":"https://openalex.org/keywords/digital-pcr","display_name":"digital PCR","score":0.8799999952316284},{"id":"https://openalex.org/keywords/margaritifera-margaritifera","display_name":"Margaritifera margaritifera","score":0.9900000095367432},{"id":"https://openalex.org/keywords/pseudanodonta-complanata","display_name":"Pseudanodonta complanata","score":0.9879999756813049},{"id":"https://openalex.org/keywords/unio-pictorum","display_name":"Unio pictorum","score":0.9990000128746033},{"id":"https://openalex.org/keywords/unio-crassus","display_name":"Unio crassus","score":1.0},{"id":"https://openalex.org/keywords/unionid-mussels","display_name":"Unionidae","score":0.8519999980926514},{"id":"https://openalex.org/keywords/margaritiferidae","display_name":"Margaritiferidae","score":0.9580000042915344},{"id":"https://openalex.org/keywords/species-detection","display_name":"species detection","score":0.5339999794960022},{"id":"https://openalex.org/keywords/detection-probability","display_name":"detection probability","score":0.6779999732971191}],"concepts":[{"id":"https://openalex.org/C179345059","wikidata":"https://www.wikidata.org/wiki/Q11394","display_name":"Endangered species","level":3,"score":0.8458750247955322},{"id":"https://openalex.org/C505870484","wikidata":"https://www.wikidata.org/wiki/Q180538","display_name":"Fishery","level":1,"score":0.47789323329925537},{"id":"https://openalex.org/C90856448","wikidata":"https://www.wikidata.org/wiki/Q431","display_name":"Zoology","level":1,"score":0.32936960458755493},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.31992948055267334},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.2885894775390625},{"id":"https://openalex.org/C185933670","wikidata":"https://www.wikidata.org/wiki/Q52105","display_name":"Habitat","level":2,"score":0.16140010952949524}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1002/edn3.70046","doi":"https://doi.org/10.1002/edn3.70046","is_oa":true,"landing_page_url":"https://doi.org/10.1002/edn3.70046","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/edn3.70046","source":{"id":"https://openalex.org/S4210176772","display_name":"Environmental DNA","issn_l":"2637-4943","issn":["2637-4943"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-1","norway-1","poland-20","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Environmental DNA","raw_type":"journal-article"},{"id":"pmh:oai:doaj.org/article:b1cfd1ef880c453eaa297db10ce47395","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/b1cfd1ef880c453eaa297db10ce47395","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Environmental DNA, Vol 6, Iss 6, Pp n/a-n/a (2024)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1002/edn3.70046","doi":"https://doi.org/10.1002/edn3.70046","is_oa":true,"landing_page_url":"https://doi.org/10.1002/edn3.70046","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/edn3.70046","source":{"id":"https://openalex.org/S4210176772","display_name":"Environmental DNA","issn_l":"2637-4943","issn":["2637-4943"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-1","norway-1","poland-20","russia-white-list-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Environmental DNA","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/14","score":0.5769000053405762,"display_name":"Life below water"},{"id":"https://metadata.un.org/sdg/6","score":0.53329998254776,"display_name":"Clean water and sanitation"}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320311297","display_name":"Havs- och Vattenmyndigheten","ror":"https://ror.org/00ht4dp10"},{"id":"https://openalex.org/F4320322579","display_name":"Naturv\u00e5rdsverket","ror":"https://ror.org/02y7nf053"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4405113483.pdf","grobid_xml":"https://content.openalex.org/works/W4405113483.grobid-xml"},"referenced_works_count":43,"referenced_works":["https://openalex.org/W1592535224","https://openalex.org/W1815195908","https://openalex.org/W2004629729","https://openalex.org/W2032019813","https://openalex.org/W2088695664","https://openalex.org/W2093478636","https://openalex.org/W2137284176","https://openalex.org/W2210031741","https://openalex.org/W2331239048","https://openalex.org/W2519928782","https://openalex.org/W2538761605","https://openalex.org/W2605440753","https://openalex.org/W2755985326","https://openalex.org/W2769897367","https://openalex.org/W2782686024","https://openalex.org/W2783918170","https://openalex.org/W2794220083","https://openalex.org/W2935925248","https://openalex.org/W2951968263","https://openalex.org/W2959499736","https://openalex.org/W2974095620","https://openalex.org/W2981337668","https://openalex.org/W2982659760","https://openalex.org/W2984503804","https://openalex.org/W2996057874","https://openalex.org/W3016113032","https://openalex.org/W3036144229","https://openalex.org/W3037509738","https://openalex.org/W3046124896","https://openalex.org/W3107492607","https://openalex.org/W3137682792","https://openalex.org/W3143509813","https://openalex.org/W3174937935","https://openalex.org/W4221104092","https://openalex.org/W4229054411","https://openalex.org/W4250963355","https://openalex.org/W4280575772","https://openalex.org/W4312181466","https://openalex.org/W4367675192","https://openalex.org/W4379056730","https://openalex.org/W4381251448","https://openalex.org/W4386210589","https://openalex.org/W4390449166"],"related_works":["https://openalex.org/W2198736673","https://openalex.org/W4412055933","https://openalex.org/W3084418930","https://openalex.org/W3094109263","https://openalex.org/W3111492540","https://openalex.org/W3136027312","https://openalex.org/W3196211251","https://openalex.org/W2794220083","https://openalex.org/W3004206018","https://openalex.org/W3108996096"],"abstract_inverted_index":{"ABSTRACT":[0],"Freshwater":[1],"mussels":[2,181],"are":[3,122],"traditionally":[4],"monitored":[5],"by":[6],"visual":[7,37],"observations":[8],"which":[9,121,219],"is":[10,23],"time\u2010consuming":[11],"and":[12,70,96,113,139,216,226],"can":[13,29,43,55,77],"be":[14,56,78],"difficult":[15],"depending":[16],"on":[17,213],"water":[18,35,217],"conditions.":[19],"Environmental":[20],"DNA":[21,42,209],"(eDNA)":[22],"an":[24],"attractive":[25],"alternative":[26],"since":[27,40],"it":[28],"detect":[30],"a":[31,53,200],"species":[32,54,98,136,197],"in":[33,47,126,141,150,156,199,231],"the":[34,41,48,61,87,102,108,114,133,151,164,178,187,192,207,214,223,229],"without":[36],"observations.":[38],"Furthermore,":[39,170],"potentially":[44],"travel":[45],"downstream":[46],"river":[49,104,117],"systems,":[50],"presence":[51,176],"of":[52,63,163,177,186,228,233],"detected":[57],"even":[58],"away":[59],"from":[60,93,99],"population":[62,234],"mussels.":[64],"In":[65],"this":[66],"paper,":[67],"we":[68],"evaluate":[69],"describe":[71],"how":[72],"dPCR":[73,130,201],"(digital":[74],"PCR)":[75],"technique":[76],"used":[79],"to":[80,194],"efficiently":[81],"monitor":[82],"four":[83,134,188],"freshwater":[84,88,165,180],"mussel":[85,90,105,110,118,135,167,189],"species:":[86],"pearl":[89,166],"Margaritifera":[91],"margaritifera":[92],"Margaritiferidae":[94],"family":[95],"three":[97],"Unionidae":[100],"family:":[101],"depressed":[103],"Pseudanodonta":[106],"complanata,":[107],"painter's":[109],"Unio":[111,119],"pictorum,":[112],"thick":[115],"shelled":[116],"crassus,":[120],"all":[123],"regarded":[124],"endangered":[125,179],"many":[127],"regions":[128],"worldwide.":[129],"assays":[131],"for":[132,184],"were":[137,182],"developed":[138],"tested":[140],"silico":[142],"before":[143],"conducting":[144],"field":[145,152],"surveys.":[146],"The":[147],"verification":[148],"step":[149],"was":[153],"carried":[154],"out":[155],"two":[157,171],"Swedish":[158],"rivers":[159,173],"with":[160,174],"confirmed":[161],"populations":[162],"(M.":[168],"margaritifera).":[169],"other":[172],"unknown":[175],"surveyed":[183],"occurrence":[185],"species,":[190],"using":[191],"capacity":[193],"multiplex":[195],"several":[196],"simultaneously":[198],"reaction.":[202],"We":[203],"furthermore":[204],"showed":[205],"that":[206],"target":[208],"concentrations":[210],"probably":[211],"depend":[212],"season":[215],"level,":[218],"may":[220],"largely":[221],"affect":[222],"detection":[224],"probability":[225],"interpretation":[227],"results":[230],"terms":[232],"size.":[235]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":1}],"updated_date":"2026-11-05T12:25:35.769452","created_date":"2024-12-07T00:00:00"}
