{"id":"https://openalex.org/W4405040586","doi":"https://doi.org/10.1182/blood-2024-206939","title":"Parallel Sequencing of Transposons and Transcriptomes in Single Cells for Genome-Wide Cancer Gene Discovery","display_name":"Parallel Sequencing of Transposons and Transcriptomes in Single Cells for Genome-Wide Cancer Gene Discovery","publication_year":2024,"publication_date":"2024-11-05","ids":{"openalex":"https://openalex.org/W4405040586","doi":"https://doi.org/10.1182/blood-2024-206939"},"language":"en","primary_location":{"id":"doi:10.1182/blood-2024-206939","doi":"https://doi.org/10.1182/blood-2024-206939","is_oa":false,"landing_page_url":"https://doi.org/10.1182/blood-2024-206939","pdf_url":null,"source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5010278690","display_name":"Maximilian Holz","orcid":"https://orcid.org/0009-0002-1294-8205","observed_orcids":["https://orcid.org/0009-0002-1294-8205"]},"institutions":[{"id":"https://openalex.org/I205582932","display_name":"Max Delbr\u00fcck Center","ror":"https://ror.org/04p5ggc03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I205582932"]},{"id":"https://openalex.org/I4392738228","display_name":"Stiftung Charit\u00e9","ror":"https://ror.org/016rf0n60","country_code":null,"type":"nonprofit","lineage":["https://openalex.org/I4392738228","https://openalex.org/I7877124"]},{"id":"https://openalex.org/I7877124","display_name":"Charit\u00e9 - Universit\u00e4tsmedizin Berlin","ror":"https://ror.org/001w7jn25","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I7877124"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Maximilian Holz","raw_affiliation_strings":["1Max-Delbr\u00fcck-Center for Molecular Medicine, Berlin, Germany","2Department of Hematology, Oncology and Cancer Immunology, Campus Benjamin Franklin, Charit\u00e9, Berlin, DEU"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Max-Delbr\u00fcck-Center for Molecular Medicine, Berlin, Germany","institution_ids":["https://openalex.org/I205582932"]},{"raw_affiliation_string":"2Department of Hematology, Oncology and Cancer Immunology, Campus Benjamin Franklin, Charit\u00e9, Berlin, DEU","institution_ids":["https://openalex.org/I4392738228","https://openalex.org/I7877124"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5084856882","display_name":"Bertram Klinger","orcid":"https://orcid.org/0000-0003-0242-1506","observed_orcids":["https://orcid.org/0000-0003-0242-1506"]},"institutions":[{"id":"https://openalex.org/I39343248","display_name":"Humboldt-Universit\u00e4t zu Berlin","ror":"https://ror.org/01hcx6992","country_code":"DE","type":"education","lineage":["https://openalex.org/I39343248"]},{"id":"https://openalex.org/I7877124","display_name":"Charit\u00e9 - Universit\u00e4tsmedizin Berlin","ror":"https://ror.org/001w7jn25","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I7877124"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Bertram Klinger","raw_affiliation_strings":["3Institute of Pathology, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Berlin, Germany","4Institute for Theoretical Biology, Humboldt-Universit\u00e4t zu Berlin, Berlin, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"3Institute of Pathology, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Berlin, Germany","institution_ids":["https://openalex.org/I7877124"]},{"raw_affiliation_string":"4Institute for Theoretical Biology, Humboldt-Universit\u00e4t zu Berlin, Berlin, Germany","institution_ids":["https://openalex.org/I39343248"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5115006329","display_name":"Felix Stuckenbrok","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205582932","display_name":"Max Delbr\u00fcck Center","ror":"https://ror.org/04p5ggc03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I205582932"]},{"id":"https://openalex.org/I4210093919","display_name":"Munich Leukemia Laboratory (Germany)","ror":"https://ror.org/00smdp487","country_code":"DE","type":"company","lineage":["https://openalex.org/I4210093919"]},{"id":"https://openalex.org/I7877124","display_name":"Charit\u00e9 - Universit\u00e4tsmedizin Berlin","ror":"https://ror.org/001w7jn25","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I7877124"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Felix Stuckenbrok","raw_affiliation_strings":["1Max-Delbr\u00fcck-Center for Molecular Medicine, Berlin, Germany","5Department of Hematology, Oncology and Cancer Immunology, Campus Benjamin Franklin, Charit\u00e9 - Universit\u00e4tsmedizin Berlin, Berlin, Germany","6MLL Munich Leukemia Laboratory, Munich, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Max-Delbr\u00fcck-Center for Molecular Medicine, Berlin, Germany","institution_ids":["https://openalex.org/I205582932"]},{"raw_affiliation_string":"5Department of Hematology, Oncology and Cancer Immunology, Campus Benjamin Franklin, Charit\u00e9 - Universit\u00e4tsmedizin Berlin, Berlin, Germany","institution_ids":["https://openalex.org/I7877124"]},{"raw_affiliation_string":"6MLL Munich Leukemia Laboratory, Munich, Germany","institution_ids":["https://openalex.org/I4210093919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010440924","display_name":"Stefanos A. Bamopoulos","orcid":"https://orcid.org/0000-0002-5997-7735","observed_orcids":["https://orcid.org/0000-0002-5997-7735"]},"institutions":[{"id":"https://openalex.org/I205582932","display_name":"Max Delbr\u00fcck Center","ror":"https://ror.org/04p5ggc03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I205582932"]},{"id":"https://openalex.org/I7877124","display_name":"Charit\u00e9 - Universit\u00e4tsmedizin Berlin","ror":"https://ror.org/001w7jn25","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I7877124"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Stefanos A. Bamopoulos","raw_affiliation_strings":["7Max-Delbr\u00fcck-Center for Molecular Medicine, Berlin, Germany","8Department of Hematology, Oncology and Tumor Immunology (Campus Benjamin Franklin), Charit\u00e9 University Medicine Berlin, Berlin, DEU"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"7Max-Delbr\u00fcck-Center for Molecular Medicine, Berlin, Germany","institution_ids":["https://openalex.org/I205582932"]},{"raw_affiliation_string":"8Department of Hematology, Oncology and Tumor Immunology (Campus Benjamin Franklin), Charit\u00e9 University Medicine Berlin, Berlin, DEU","institution_ids":["https://openalex.org/I7877124"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103059261","display_name":"Sanket Gosavi","orcid":"https://orcid.org/0009-0002-7656-9685","observed_orcids":["https://orcid.org/0009-0002-7656-9685"]},"institutions":[{"id":"https://openalex.org/I7877124","display_name":"Charit\u00e9 - Universit\u00e4tsmedizin Berlin","ror":"https://ror.org/001w7jn25","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I7877124"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Sanket Gosavi","raw_affiliation_strings":["5Department of Hematology, Oncology and Cancer Immunology, Campus Benjamin Franklin, Charit\u00e9 - Universit\u00e4tsmedizin Berlin, Berlin, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"5Department of Hematology, Oncology and Cancer Immunology, Campus Benjamin Franklin, Charit\u00e9 - Universit\u00e4tsmedizin Berlin, Berlin, Germany","institution_ids":["https://openalex.org/I7877124"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076529951","display_name":"Caroline Braeuning","orcid":"https://orcid.org/0009-0007-1439-9920","observed_orcids":["https://orcid.org/0009-0007-1439-9920"]},"institutions":[{"id":"https://openalex.org/I205582932","display_name":"Max Delbr\u00fcck Center","ror":"https://ror.org/04p5ggc03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I205582932"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Caroline Br\u00e4uning","raw_affiliation_strings":["9Genomics Technology Platform, Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"9Genomics Technology Platform, Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany","institution_ids":["https://openalex.org/I205582932"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038373469","display_name":"Janine Altm\u00fcller","orcid":"https://orcid.org/0000-0003-4372-1521","observed_orcids":["https://orcid.org/0000-0003-4372-1521","https://orcid.org/0000-0001-9136-6902"]},"institutions":[{"id":"https://openalex.org/I205582932","display_name":"Max Delbr\u00fcck Center","ror":"https://ror.org/04p5ggc03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I205582932"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Janine Altm\u00fcller","raw_affiliation_strings":["9Genomics Technology Platform, Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"9Genomics Technology Platform, Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany","institution_ids":["https://openalex.org/I205582932"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082231210","display_name":"Roland Rad","orcid":"https://orcid.org/0000-0002-6849-9659","observed_orcids":["https://orcid.org/0000-0002-6849-9659"]},"institutions":[{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Roland Rad","raw_affiliation_strings":["10Institute of Molecular Oncology and Functional Genomics, Technical University of Munich, Munich, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"10Institute of Molecular Oncology and Functional Genomics, Technical University of Munich, Munich, Germany","institution_ids":["https://openalex.org/I62916508"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5043414993","display_name":"Torsten Haferlach","orcid":"https://orcid.org/0000-0003-0196-2837","observed_orcids":["https://orcid.org/0000-0003-0196-2837"]},"institutions":[{"id":"https://openalex.org/I4210093919","display_name":"Munich Leukemia Laboratory (Germany)","ror":"https://ror.org/00smdp487","country_code":"DE","type":"company","lineage":["https://openalex.org/I4210093919"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Torsten Haferlach","raw_affiliation_strings":["6MLL Munich Leukemia Laboratory, Munich, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"6MLL Munich Leukemia Laboratory, Munich, Germany","institution_ids":["https://openalex.org/I4210093919"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078717142","display_name":"Nils Bl\u00fcthgen","orcid":"https://orcid.org/0000-0002-0171-7447","observed_orcids":["https://orcid.org/0000-0002-0171-7447"]},"institutions":[{"id":"https://openalex.org/I17937529","display_name":"German Cancer Research Center","ror":"https://ror.org/04cdgtt98","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I17937529"]},{"id":"https://openalex.org/I39343248","display_name":"Humboldt-Universit\u00e4t zu Berlin","ror":"https://ror.org/01hcx6992","country_code":"DE","type":"education","lineage":["https://openalex.org/I39343248"]},{"id":"https://openalex.org/I7877124","display_name":"Charit\u00e9 - Universit\u00e4tsmedizin Berlin","ror":"https://ror.org/001w7jn25","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I7877124"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Nils Bl\u00fcthgen","raw_affiliation_strings":["11Institute of Pathology, Charit\u00e9-Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, Berlin, Germany","12German Cancer Research Center (DKFZ), German Cancer Consortium (DKTK), Berlin, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"11Institute of Pathology, Charit\u00e9-Universit\u00e4tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin and Humboldt-Universit\u00e4t zu Berlin, Berlin, Germany","institution_ids":["https://openalex.org/I39343248","https://openalex.org/I7877124"]},{"raw_affiliation_string":"12German Cancer Research Center (DKFZ), German Cancer Consortium (DKTK), Berlin, Germany","institution_ids":["https://openalex.org/I17937529"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028510513","display_name":"Ulrich Bernd Keller","orcid":"https://orcid.org/0000-0002-8485-1958","observed_orcids":["https://orcid.org/0000-0002-8485-1958"]},"institutions":[{"id":"https://openalex.org/I17937529","display_name":"German Cancer Research Center","ror":"https://ror.org/04cdgtt98","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I17937529"]},{"id":"https://openalex.org/I205582932","display_name":"Max Delbr\u00fcck Center","ror":"https://ror.org/04p5ggc03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I205582932"]},{"id":"https://openalex.org/I4391767962","display_name":"Deutsches Konsortium f\u00fcr Translationale Krebsforschung","ror":"https://ror.org/02pqn3g31","country_code":"DE","type":"facility","lineage":["https://openalex.org/I4391767962","https://openalex.org/I4405256427"]},{"id":"https://openalex.org/I7877124","display_name":"Charit\u00e9 - Universit\u00e4tsmedizin Berlin","ror":"https://ror.org/001w7jn25","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I7877124"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Ulrich Keller","raw_affiliation_strings":["13German Cancer Consortium (DKTK) and German Cancer Research Center (DKFZ), Heidelberg, Germany","14Max-Delbr\u00fcck-Center, Berlin, Germany","15Hematology, Oncology and Cancer Immunology, Charit\u00e9 - University Medical Center Berlin, Campus Benjamin Franklin, Berlin, Germany","16German Cancer Consortium (DKTK) partner site Berlin, a partnership between DKFZ and Charit\u00e9 - Universit\u00e4tsmedizin Berlin, Berlin, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"13German Cancer Consortium (DKTK) and German Cancer Research Center (DKFZ), Heidelberg, Germany","institution_ids":["https://openalex.org/I17937529","https://openalex.org/I4391767962"]},{"raw_affiliation_string":"14Max-Delbr\u00fcck-Center, Berlin, Germany","institution_ids":["https://openalex.org/I205582932"]},{"raw_affiliation_string":"15Hematology, Oncology and Cancer Immunology, Charit\u00e9 - University Medical Center Berlin, Campus Benjamin Franklin, Berlin, Germany","institution_ids":["https://openalex.org/I7877124"]},{"raw_affiliation_string":"16German Cancer Consortium (DKTK) partner site Berlin, a partnership between DKFZ and Charit\u00e9 - Universit\u00e4tsmedizin Berlin, Berlin, Germany","institution_ids":["https://openalex.org/I7877124"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065978869","display_name":"Thomas Conrad","orcid":"https://orcid.org/0000-0001-5618-6295","observed_orcids":["https://orcid.org/0000-0001-5618-6295"]},"institutions":[{"id":"https://openalex.org/I205582932","display_name":"Max Delbr\u00fcck Center","ror":"https://ror.org/04p5ggc03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I205582932"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Thomas Conrad","raw_affiliation_strings":["17Genomics Technology Platform, Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association, Berlin, DEU"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"17Genomics Technology Platform, Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association, Berlin, DEU","institution_ids":["https://openalex.org/I205582932"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5059762155","display_name":"Francis Baumgartner","orcid":"https://orcid.org/0000-0003-0768-1956","observed_orcids":["https://orcid.org/0000-0003-0768-1956"]},"institutions":[{"id":"https://openalex.org/I17937529","display_name":"German Cancer Research Center","ror":"https://ror.org/04cdgtt98","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I17937529"]},{"id":"https://openalex.org/I205582932","display_name":"Max Delbr\u00fcck Center","ror":"https://ror.org/04p5ggc03","country_code":"DE","type":"facility","lineage":["https://openalex.org/I1305996414","https://openalex.org/I205582932"]},{"id":"https://openalex.org/I4210093919","display_name":"Munich Leukemia Laboratory (Germany)","ror":"https://ror.org/00smdp487","country_code":"DE","type":"company","lineage":["https://openalex.org/I4210093919"]},{"id":"https://openalex.org/I7877124","display_name":"Charit\u00e9 - Universit\u00e4tsmedizin Berlin","ror":"https://ror.org/001w7jn25","country_code":"DE","type":"healthcare","lineage":["https://openalex.org/I7877124"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Francis Baumgartner","raw_affiliation_strings":["12German Cancer Research Center (DKFZ), German Cancer Consortium (DKTK), Berlin, Germany","1Max-Delbr\u00fcck-Center for Molecular Medicine, Berlin, Germany","5Department of Hematology, Oncology and Cancer Immunology, Campus Benjamin Franklin, Charit\u00e9 - Universit\u00e4tsmedizin Berlin, Berlin, Germany","6MLL Munich Leukemia Laboratory, Munich, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"12German Cancer Research Center (DKFZ), German Cancer Consortium (DKTK), Berlin, Germany","institution_ids":["https://openalex.org/I17937529"]},{"raw_affiliation_string":"1Max-Delbr\u00fcck-Center for Molecular Medicine, Berlin, Germany","institution_ids":["https://openalex.org/I205582932"]},{"raw_affiliation_string":"5Department of Hematology, Oncology and Cancer Immunology, Campus Benjamin Franklin, Charit\u00e9 - Universit\u00e4tsmedizin Berlin, Berlin, Germany","institution_ids":["https://openalex.org/I7877124"]},{"raw_affiliation_string":"6MLL Munich Leukemia Laboratory, Munich, Germany","institution_ids":["https://openalex.org/I4210093919"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":8,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":5000,"currency":"USD","value_usd":5000},"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"144","issue":"Supplement 1","first_page":"1351","last_page":"1351"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10309","display_name":"Acute Myeloid Leukemia Research","score":0.7685999870300293,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10309","display_name":"Acute Myeloid Leukemia Research","score":0.7685999870300293,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.07039999961853027,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11287","display_name":"Cancer Genomics and Diagnostics","score":0.052400000393390656,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/transposon-mutagenesis-screen","display_name":"transposon mutagenesis screen","score":0.6790000200271606},{"id":"https://openalex.org/keywords/single-cell-transcriptomics","display_name":"single-cell transcriptomics","score":0.7110000252723694},{"id":"https://openalex.org/keywords/single-cell-sequencing","display_name":"single-cell sequencing","score":0.4390000104904175},{"id":"https://openalex.org/keywords/acute-myeloid-leukemia","display_name":"acute myeloid leukemia","score":0.9369999766349792},{"id":"https://openalex.org/keywords/asxl1-mutation","display_name":"ASXL1 mutation","score":0.8220000267028809},{"id":"https://openalex.org/keywords/cancer-gene-discovery","display_name":"cancer gene discovery","score":0.8270000219345093},{"id":"https://openalex.org/keywords/clonal-evolution","display_name":"clonal evolution","score":0.7829999923706055},{"id":"https://openalex.org/keywords/intratumor-heterogeneity","display_name":"intratumoral heterogeneity","score":0.9940000176429749},{"id":"https://openalex.org/keywords/prdm16","display_name":"PRDM16","score":0.6919999718666077},{"id":"https://openalex.org/keywords/mecom","display_name":"MECOM","score":0.9919999837875366},{"id":"https://openalex.org/keywords/dock5","display_name":"DOCK5","score":0.843999981880188},{"id":"https://openalex.org/keywords/tcf4","display_name":"TCF4","score":0.9279999732971191},{"id":"https://openalex.org/keywords/csf-1","display_name":"CSF-1","score":0.9879999756813049},{"id":"https://openalex.org/keywords/multiple-displacement-amplification","display_name":"multiple displacement amplification","score":0.5329999923706055}],"concepts":[{"id":"https://openalex.org/C4918238","wikidata":"https://www.wikidata.org/wiki/Q121438","display_name":"Transposable element","level":4,"score":0.8287973403930664},{"id":"https://openalex.org/C28693550","wikidata":"https://www.wikidata.org/wiki/Q7835335","display_name":"Transposon mutagenesis","level":5,"score":0.7479779720306396},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7353832125663757},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.630790650844574},{"id":"https://openalex.org/C162317418","wikidata":"https://www.wikidata.org/wiki/Q252857","display_name":"Transcriptome","level":4,"score":0.5657645463943481},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.551124095916748},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5500987768173218},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5252527594566345},{"id":"https://openalex.org/C16318435","wikidata":"https://www.wikidata.org/wiki/Q149299","display_name":"Mutagenesis","level":4,"score":0.5202825665473938},{"id":"https://openalex.org/C107635520","wikidata":"https://www.wikidata.org/wiki/Q1967554","display_name":"Insertional mutagenesis","level":4,"score":0.47024837136268616},{"id":"https://openalex.org/C205270622","wikidata":"https://www.wikidata.org/wiki/Q17082639","display_name":"Cancer genome sequencing","level":5,"score":0.4461126923561096},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.3642173409461975},{"id":"https://openalex.org/C189206191","wikidata":"https://www.wikidata.org/wiki/Q222046","display_name":"Genomics","level":4,"score":0.35399919748306274},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.2038104236125946}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1182/blood-2024-206939","doi":"https://doi.org/10.1182/blood-2024-206939","is_oa":false,"landing_page_url":"https://doi.org/10.1182/blood-2024-206939","pdf_url":null,"source":{"id":"https://openalex.org/S200071133","display_name":"Blood","issn_l":"0006-4971","issn":["0006-4971","1528-0020"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Blood","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9476000070571899,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[{"score":0.4099999964237213,"display_name":"No poverty","id":"https://metadata.un.org/sdg/1"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2072376324","https://openalex.org/W2040654308","https://openalex.org/W2336350895","https://openalex.org/W2144107911","https://openalex.org/W2392664193","https://openalex.org/W2397734173","https://openalex.org/W2141794711","https://openalex.org/W2015745707","https://openalex.org/W2124561494","https://openalex.org/W1486248635"],"abstract_inverted_index":{"Introduction":[0],"Genome-wide":[1],"transposon":[2,48,130,166,399,407],"mutagenesis":[3,167],"screens":[4,400],"in":[5,19,168,205,275,373,379,409,425],"mice":[6],"are":[7],"powerful":[8],"tools":[9],"for":[10,31,182,221,335],"identifying":[11],"genomically,":[12],"transcriptionally,":[13],"and":[14,35,38,59,82,121,143,218,242,260,262,278,324,350,353,422,435],"epigenetically":[15],"deregulated":[16],"cancer":[17,85],"genes":[18],"a":[20,63,105,139,177,305,332,380],"single":[21,55,76,113,410],"experimental":[22],"approach.":[23],"A":[24,89],"major":[25],"limitation,":[26],"however,":[27],"is":[28],"the":[29,68,75,161,174,213,228,237,290,310,364,417,438],"need":[30],"large":[32,178],"cohort":[33],"sizes":[34],"substantial":[36],"sequencing":[37,281],"bioinformatic":[39],"resources":[40],"to":[41,97,173,232,268,309,363,397],"differentiate":[42],"causal":[43],"genetic":[44],"changes":[45],"from":[46,116,127,160,283,301],"silent":[47],"insertions.":[49],"We":[50],"address":[51],"these":[52],"challenges":[53],"with":[54,107,156,198,254],"cell":[56,77],"PiggyBac":[57,91],"Transposon":[58],"Transcriptome":[60],"Sequencing":[61],"(scPB&T-seq),":[62],"novel":[64,264],"dual-assay":[65],"that":[66,401],"enables":[67,416],"identification":[69,175,243],"of":[70,84,176,192,212,239,244,313,320,329,383,412,419,432,440],"disrupted":[71],"molecular":[72],"networks":[73,424],"at":[74],"level,":[78],"thus":[79],"improving":[80],"precision":[81],"efficacy":[83],"gene":[86,180,216],"studies.":[87],"Methods":[88],"murine":[90],"(PB)":[92],"transposition":[93],"screen":[94],"was":[95,124,153,375],"performed":[96],"generate":[98],"Asxl1-mutated":[99],"(Asxl1mut)":[100],"acute":[101],"myeloid":[102],"leukemias":[103],"(AMLs),":[104],"subgroup":[106,328],"particularly":[108],"poor":[109],"prognosis.":[110],"For":[111],"scPB&T-seq,":[112,393],"cells":[114,411],"(sc)":[115],"three":[117],"AMLs":[118],"were":[119,132,203,219,230,273],"isolated,":[120],"genomic":[122,265],"DNA":[123],"physically":[125],"separated":[126],"mRNA.":[128],"Sc":[129],"insertions":[131,202,214,229,408],"amplified":[133],"via":[134],"multiple":[135],"displacement":[136],"followed":[137],"by":[138,149],"custom":[140],"PCR":[141],"amplification":[142],"sequenced":[144],"alongside":[145],"sc":[146],"transcriptomes":[147],"derived":[148],"Smart-Seq2.":[150],"The":[151],"data":[152,282],"then":[154],"integrated":[155],"high-throughput":[157],"droplet-based":[158],"scRNAseq":[159,226],"same":[162],"samples.":[163],"Results":[164],"Activating":[165],"an":[169,190,314,318,371,376,394],"Asxl1mut":[170,183,315,385],"background":[171],"led":[172],"candidate":[179],"catalogue":[181],"AML":[184,251,269,286,367,433],"pathogenesis.":[185,270],"Using":[186],"scPB&T-seq":[187],"we":[188,391],"identified":[189],"average":[191],"10-25":[193],"transposons":[194],"per":[195],"cell,":[196],"aligning":[197],"expected":[199],"frequencies.":[200],"All":[201],"confirmed":[204,249],"concurrent":[206,351],"bulk":[207],"insertion":[208,372,378],"analyses.":[209],"About":[210],"60%":[211],"impacted":[215],"expression":[217],"retained":[220],"further":[222],"analysis.":[223],"By":[224],"integrating":[225],"data,":[227],"assigned":[231],"specific":[233],"cellular":[234],"clusters,":[235],"allowing":[236],"reconstruction":[238],"clonal":[240,420],"evolution":[241,421],"intratumoral":[245],"heterogeneity.":[246],"This":[247,415],"approach":[248],"known":[250],"drivers":[252],"co-occurring":[253],"ASXL1":[255,336,352],"mutations,":[256],"such":[257],"as":[258],"PRDM16":[259],"MECOM,":[261],"revealed":[263],"loci":[266],"linked":[267],"These":[271],"findings":[272],"validated":[274],"whole":[276,279],"genome":[277],"transcriptome":[280],"774":[284],"human":[285],"patients":[287,300,330],"generated":[288],"within":[289],"Munich":[291],"Leukemia":[292],"Laboratory":[293],"5K":[294],"Genomes":[295],"Project.":[296],"Interestingly,":[297],"45":[298],"(5.9%)":[299],"this":[302,327,384,389],"dataset":[303],"exhibited":[304],"transcriptional":[306],"profile":[307],"similar":[308],"main":[311],"clone":[312],"PB-AML,":[316],"showcasing":[317],"upregulation":[319],"e.g.,":[321],"DOCK5,":[322],"TCF4,":[323],"CSF1.":[325],"Furthermore,":[326],"displayed":[331],"significant":[333],"enrichment":[334],"(31.1%":[337],"vs.":[338,345,356],"14.5%,":[339],"P":[340,347,358],"=":[341,348,359],"0.005),":[342],"NRAS":[343,354],"(28.9%":[344],"15.6%,":[346],"0.035)":[349],"(13.3%":[355],"3%,":[357],"0.004)":[360],"mutations":[361],"compared":[362],"remaining":[365],"724":[366],"patients.":[368],"Of":[369],"note,":[370],"Nras":[374],"activating":[377],"monocytic-like":[381],"subclone":[382],"PB-AML.":[386],"Conclusion":[387],"In":[388],"study":[390],"developed":[392],"innovative":[395],"method":[396],"analyze":[398],"uncovers":[402],"functional":[403],"mechanisms":[404],"behind":[405],"individual":[406],"heterogenous":[413],"tumors.":[414],"assessment":[418],"oncogenic":[423],"unprecedented":[426],"detail,":[427],"thereby":[428],"enhancing":[429],"our":[430],"understanding":[431],"pathogenesis":[434],"potentially":[436],"guiding":[437],"development":[439],"targeted":[441],"therapies.":[442]},"counts_by_year":[],"updated_date":"2026-10-12T09:40:04.860316","created_date":"2025-10-10T00:00:00"}
