{"id":"https://openalex.org/W4404423320","doi":"https://doi.org/10.1158/1078-0432.c.7541220","title":"Data from The Epstein\u2013Barr Virus Nuclear Antigen 1 Variant Associated with Nasopharyngeal Carcinoma Defines the Sequence Criteria for Serologic Risk Prediction","display_name":"Data from The Epstein\u2013Barr Virus Nuclear Antigen 1 Variant Associated with Nasopharyngeal Carcinoma Defines the Sequence Criteria for Serologic Risk Prediction","publication_year":2024,"publication_date":"2024-11-15","ids":{"openalex":"https://openalex.org/W4404423320","doi":"https://doi.org/10.1158/1078-0432.c.7541220"},"language":"en","primary_location":{"id":"doi:10.1158/1078-0432.c.7541220","doi":"https://doi.org/10.1158/1078-0432.c.7541220","is_oa":true,"landing_page_url":"https://doi.org/10.1158/1078-0432.c.7541220","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"American Association for Cancer Research","raw_type":"posted-content"},"volume":null,"type":"other","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1158/1078-0432.c.7541220","any_repository_has_fulltext":null},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5087885661","display_name":"Benjamin E. Warner","orcid":"https://orcid.org/0000-0002-1550-1516","observed_orcids":["https://orcid.org/0000-0002-1550-1516"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Benjamin E. Warner","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-1550-1516","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057285902","display_name":"Japan Patel","orcid":"https://orcid.org/0009-0008-7610-1840","observed_orcids":["https://orcid.org/0009-0008-7610-1840"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Japan Patel","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0009-0008-7610-1840","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063230233","display_name":"Renwei Wang","orcid":"https://orcid.org/0000-0001-8871-7054","observed_orcids":["https://orcid.org/0000-0001-8871-7054"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Renwei Wang","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0001-8871-7054","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068308715","display_name":"Jennifer\u00a0M. Adams\u2010Haduch","orcid":"https://orcid.org/0000-0002-0640-3076","observed_orcids":["https://orcid.org/0000-0002-0640-3076"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jennifer Adams-Haduch","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-0640-3076","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080361653","display_name":"Yu\u2010Tang Gao","orcid":"https://orcid.org/0000-0003-3904-1777","observed_orcids":["https://orcid.org/0000-0003-3904-1777"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yu-Tang Gao","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0003-3904-1777","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019713852","display_name":"Woon\u2010Puay Koh","orcid":"https://orcid.org/0000-0002-5674-6341","observed_orcids":["https://orcid.org/0000-0002-5674-6341"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Woon-Puay Koh","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-5674-6341","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080540757","display_name":"Ka Wo Wong","orcid":"https://orcid.org/0009-0006-4042-7529","observed_orcids":["https://orcid.org/0009-0006-4042-7529"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ka Wo Wong","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0009-0006-4042-7529","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010231188","display_name":"Aks Chiang","orcid":"https://orcid.org/0000-0002-1089-5325","observed_orcids":["https://orcid.org/0000-0002-1089-5325"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Alan K.S. Chiang","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-1089-5325","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081606775","display_name":"Jian\u2010Min Yuan","orcid":"https://orcid.org/0000-0002-4620-3108","observed_orcids":["https://orcid.org/0000-0002-4620-3108"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jian-Min Yuan","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-4620-3108","affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5078828367","display_name":"Kathy H.Y. Shair","orcid":"https://orcid.org/0000-0002-9556-1745","observed_orcids":["https://orcid.org/0000-0002-9556-1745"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kathy H.Y. Shair","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-9556-1745","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10685","display_name":"Viral-associated cancers and disorders","score":0.9243000149726868,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10685","display_name":"Viral-associated cancers and disorders","score":0.9243000149726868,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10307","display_name":"Head and Neck Cancer Studies","score":0.06340000033378601,"subfield":{"id":"https://openalex.org/subfields/2733","display_name":"Otorhinolaryngology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10340","display_name":"Hepatitis B Virus Studies","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/2713","display_name":"Epidemiology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/epstein-barr-virus","display_name":"Epstein-Barr virus","score":0.824999988079071},{"id":"https://openalex.org/keywords/ebna1","display_name":"EBNA1","score":0.9229999780654907},{"id":"https://openalex.org/keywords/nasopharyngeal-carcinoma","display_name":"nasopharyngeal carcinoma","score":0.9940000176429749},{"id":"https://openalex.org/keywords/iga-antibodies","display_name":"IgA antibodies","score":0.8230000138282776},{"id":"https://openalex.org/keywords/immunodominant-epitopes","display_name":"immunodominant epitopes","score":0.777999997138977},{"id":"https://openalex.org/keywords/early-cancer-detection","display_name":"early cancer detection","score":0.7379999756813049},{"id":"https://openalex.org/keywords/risk-stratification","display_name":"risk stratification","score":0.6980000138282776},{"id":"https://openalex.org/keywords/immunoblot-assay","display_name":"immunoblot assay","score":0.5870000123977661},{"id":"https://openalex.org/keywords/sojourn-time","display_name":"sojourn time","score":0.6000000238418579}],"concepts":[{"id":"https://openalex.org/C2778997737","wikidata":"https://www.wikidata.org/wiki/Q1693598","display_name":"Nasopharyngeal carcinoma","level":3,"score":0.882213294506073},{"id":"https://openalex.org/C45189115","wikidata":"https://www.wikidata.org/wiki/Q502159","display_name":"Serology","level":3,"score":0.7372562885284424},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.5998722910881042},{"id":"https://openalex.org/C2778112365","wikidata":"https://www.wikidata.org/wiki/Q3511065","display_name":"Sequence (biology)","level":2,"score":0.49782514572143555},{"id":"https://openalex.org/C2522874641","wikidata":"https://www.wikidata.org/wiki/Q808","display_name":"Virus","level":2,"score":0.43039166927337646},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.41368231177330017},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.34644564986228943},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.2597772479057312},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.22999310493469238},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.19187572598457336},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.1489209234714508},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.13467663526535034},{"id":"https://openalex.org/C509974204","wikidata":"https://www.wikidata.org/wiki/Q180507","display_name":"Radiation therapy","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1078-0432.c.7541220","doi":"https://doi.org/10.1158/1078-0432.c.7541220","is_oa":true,"landing_page_url":"https://doi.org/10.1158/1078-0432.c.7541220","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"American Association for Cancer Research","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.1158/1078-0432.c.7541220","doi":"https://doi.org/10.1158/1078-0432.c.7541220","is_oa":true,"landing_page_url":"https://doi.org/10.1158/1078-0432.c.7541220","pdf_url":null,"source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"American Association for Cancer Research","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":1.0,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4402488512","https://openalex.org/W4361950527","https://openalex.org/W4297266637","https://openalex.org/W7171367063","https://openalex.org/W7153059923","https://openalex.org/W2042193480","https://openalex.org/W4361948541","https://openalex.org/W4362480788","https://openalex.org/W2741129234","https://openalex.org/W2025416864"],"abstract_inverted_index":{"AbstractPurpose:":[0],"Antibodies":[1],"to":[2,46,62,146,183],"select":[3],"Epstein\u2013Barr":[4,20],"virus":[5,21],"proteins":[6],"can":[7,26,70],"diagnose":[8],"early-stage":[9],"nasopharyngeal":[10],"carcinoma":[11],"(NPC).":[12],"We":[13,187],"have":[14],"previously":[15],"shown":[16],"that":[17,189],"IgA":[18,88,174,232],"against":[19],"nuclear":[22],"antigen":[23,193],"1":[24],"(EBNA1)":[25],"predict":[27],"incident":[28],"NPC":[29,53,99,141,206,247],"in":[30,76,93,105,177,203],"high-":[31],"and":[32,67,79,89,107,250],"intermediate-risk":[33],"cohorts":[34,104],"4":[35],"years":[36],"before":[37],"diagnosis.":[38],"Here,":[39],"we":[40],"tested":[41],"EBNA1":[42,64,128,164,173,192,223,231,244],"variants,":[43],"with":[44,124,199,213],"mutants,":[45],"define":[47],"the":[48,77,109,131,147,153,163,184,196,227,230,239],"sequence":[49],"requirements":[50],"for":[51,126,130,195,210,234,246],"an":[52,214],"risk":[54,211,235,248],"assay.":[55],"Experimental":[56],"Design:":[57],"Mammalian-expressed":[58],"constructs":[59],"were":[60,83],"developed":[61],"represent":[63],"variants":[65],"487V":[66,118],"487A,":[68],"which":[69],"differ":[71],"by":[72,85,139],"\u226515":[73],"amino":[74],"acids":[75],"N-":[78],"C-termini.":[80],"Denatured":[81],"lysates":[82],"evaluated":[84],"a":[86,94,120,190],"refined":[87,191],"IgG":[90],"immunoblot":[91],"assay":[92,233],"case-control":[95],"study":[96],"using":[97],"prediagnostic":[98],"sera":[100,142],"from":[101],"two":[102],"independent":[103],"Singapore":[106],"Shanghai,":[108],"People\u2019s":[110],"Republic":[111],"of":[112,218,229,241],"China.":[113],"Results:":[114],"At":[115],"95%":[116,169],"sensitivity,":[117],"yielded":[119],"94.9%":[121],"specificity":[122,160],"compared":[123],"86.1%":[125],"487A.":[127],"deleted":[129,194],"conserved":[132],"glycine\u2013alanine":[133],"repeats":[134],"(GAr)":[135],"reduced":[136],"false":[137],"positives":[138],"22.8%.":[140],"reacted":[143],"more":[144],"strongly":[145],"C-terminus":[148],"than":[149,162],"healthy":[150,178],"controls,":[151],"but":[152,198],"C-terminal":[154],"construct":[155,166],"(a.a.":[156],"390\u2013641)":[157],"showed":[158],"lower":[159],"(84.8%)":[161],"GAr\u2013deleted":[165],"(92.4%)":[167],"at":[168],"sensitivity.":[170],"Conclusions:":[171],"Although":[172],"was":[175],"present":[176],"sera,":[179],"most":[180],"epitopes":[181],"localized":[182],"immunodominant":[185],"GAr.":[186],"conclude":[188],"GAr,":[197],"residues":[200],"consistently":[201],"detected":[202],"Southeast":[204],"Asian":[205],"tumors,":[207],"is":[208],"optimized":[209],"prediction":[212,249],"extended":[215],"sojourn":[216],"time":[217],"7.5":[219],"years.":[220],"Furthermore,":[221],"distinct":[222],"serologic":[224],"profiles":[225],"enhanced":[226],"utility":[228],"stratification.":[236],"This":[237],"illustrates":[238],"importance":[240],"serologically":[242],"relevant":[243],"sequences":[245],"early":[251],"detection.":[252]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2024-11-16T00:00:00"}