{"id":"https://openalex.org/W4404064360","doi":"https://doi.org/10.1136/jitc-2024-sitc2024.1377","title":"1377 Imaging Mass Cytometry\u2122 approach unveils extensive cellular heterogeneity in human brain tumors","display_name":"1377 Imaging Mass Cytometry\u2122 approach unveils extensive cellular heterogeneity in human brain tumors","publication_year":2024,"publication_date":"2024-11-01","ids":{"openalex":"https://openalex.org/W4404064360","doi":"https://doi.org/10.1136/jitc-2024-sitc2024.1377"},"language":"en","primary_location":{"id":"doi:10.1136/jitc-2024-sitc2024.1377","doi":"https://doi.org/10.1136/jitc-2024-sitc2024.1377","is_oa":true,"landing_page_url":"https://doi.org/10.1136/jitc-2024-sitc2024.1377","pdf_url":null,"source":{"id":"https://openalex.org/S2754803477","display_name":"Journal for ImmunoTherapy of Cancer","issn_l":"2051-1426","issn":["2051-1426"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319945","host_organization_name":"BMJ","host_organization_lineage":["https://openalex.org/P4310319945"],"host_organization_lineage_names":["BMJ"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Regular and Young Investigator Award Abstracts","raw_type":"proceedings-article"},"volume":null,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1136/jitc-2024-sitc2024.1377","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044780860","display_name":"Nick Zabinyakov","orcid":"https://orcid.org/0009-0009-1234-199X","observed_orcids":["https://orcid.org/0009-0009-1234-199X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Nick Zabinyakov","raw_affiliation_strings":["Standard BioTools Canada Inc., Markham, ON, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Standard BioTools Canada Inc., Markham, ON, Canada","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5002850706","display_name":"Qanber Raza","orcid":"https://orcid.org/0000-0001-7612-805X","observed_orcids":["https://orcid.org/0000-0001-7612-805X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Qanber Raza","raw_affiliation_strings":["Standard BioTools Canada Inc., Markham, ON, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Standard BioTools Canada Inc., Markham, ON, Canada","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064476636","display_name":"Thomas Daniel Pfister","orcid":"https://orcid.org/0009-0006-9766-2984","observed_orcids":["https://orcid.org/0009-0006-9766-2984"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Thomas D Pfister","raw_affiliation_strings":["Standard BioTools Canada Inc., Markham, ON, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Standard BioTools Canada Inc., Markham, ON, Canada","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044370554","display_name":"Nikesh Parsotam","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Nikesh Parsotam","raw_affiliation_strings":["Standard BioTools Canada Inc., Markham, ON, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Standard BioTools Canada Inc., Markham, ON, Canada","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"David Howell","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"David Howell","raw_affiliation_strings":["Standard BioTools Canada Inc., Markham, ON, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Standard BioTools Canada Inc., Markham, ON, Canada","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110286197","display_name":"Liang Kuang Lim","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210097591","display_name":"Fluidigm (Canada)","ror":"https://ror.org/00xvbmj91","country_code":"CA","type":"company","lineage":["https://openalex.org/I4210097591","https://openalex.org/I922094183"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Liang Lim","raw_affiliation_strings":["Fluidigm, Markham, ON, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Fluidigm, Markham, ON, Canada","institution_ids":["https://openalex.org/I4210097591"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044220890","display_name":"Jyh Yun Chwee","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jyh Yun Chwee","raw_affiliation_strings":["Standard BioTools Canada Inc., Markham, ON, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Standard BioTools Canada Inc., Markham, ON, Canada","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5063154809","display_name":"Christina Loh","orcid":"https://orcid.org/0009-0009-6139-4895","observed_orcids":["https://orcid.org/0009-0009-6139-4895"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Christina Loh","raw_affiliation_strings":["Standard BioTools Canada Inc., Markham, ON, Canada"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Standard BioTools Canada Inc., Markham, ON, Canada","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":2490,"currency":"USD","value_usd":2490},"apc_paid":{"value":2490,"currency":"USD","value_usd":2490},"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":"A1540","last_page":"A1540"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10129","display_name":"Glioma Diagnosis and Treatment","score":0.8349999785423279,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10129","display_name":"Glioma Diagnosis and Treatment","score":0.8349999785423279,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10158","display_name":"Cancer Immunotherapy and Biomarkers","score":0.07240000367164612,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.05959999933838844,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/brain-tumors","display_name":"brain tumors","score":0.7870000004768372},{"id":"https://openalex.org/keywords/brain-tumor-microenvironment","display_name":"brain tumor microenvironment","score":0.8159999847412109},{"id":"https://openalex.org/keywords/imaging-mass-cytometry","display_name":"imaging mass cytometry","score":0.8619999885559082},{"id":"https://openalex.org/keywords/glioblastoma","display_name":"glioblastoma","score":0.8690000176429749},{"id":"https://openalex.org/keywords/astrocytoma","display_name":"astrocytoma","score":0.9869999885559082},{"id":"https://openalex.org/keywords/cell-heterogeneity","display_name":"cellular heterogeneity","score":0.8849999904632568},{"id":"https://openalex.org/keywords/tumor-immune-infiltration","display_name":"tumor immune infiltration","score":0.593999981880188},{"id":"https://openalex.org/keywords/myeloid-cells","display_name":"myeloid cells","score":0.7889999747276306},{"id":"https://openalex.org/keywords/lymphoid-cells","display_name":"lymphoid cells","score":0.781000018119812},{"id":"https://openalex.org/keywords/glioma-stem-cells","display_name":"glioma stem cells","score":0.8109999895095825},{"id":"https://openalex.org/keywords/cancer-associated-fibroblasts","display_name":"cancer-associated fibroblasts","score":0.9290000200271606},{"id":"https://openalex.org/keywords/tumor-associated-macrophages","display_name":"tumor-associated macrophages","score":0.824999988079071},{"id":"https://openalex.org/keywords/microglia","display_name":"microglia","score":0.7680000066757202},{"id":"https://openalex.org/keywords/spatial-transcriptomics","display_name":"spatial transcriptomics","score":0.44699999690055847}],"concepts":[{"id":"https://openalex.org/C2778015335","wikidata":"https://www.wikidata.org/wiki/Q17051163","display_name":"Mass cytometry","level":4,"score":0.9107517004013062},{"id":"https://openalex.org/C2780339063","wikidata":"https://www.wikidata.org/wiki/Q5201367","display_name":"Cytometry","level":3,"score":0.4866355359554291},{"id":"https://openalex.org/C2777670902","wikidata":"https://www.wikidata.org/wiki/Q492038","display_name":"Human brain","level":2,"score":0.4635445773601532},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.42817947268486023},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.40806782245635986},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.23071879148483276},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.21758824586868286},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.19000115990638733},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.17014962434768677},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.07124701142311096},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.0},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1136/jitc-2024-sitc2024.1377","doi":"https://doi.org/10.1136/jitc-2024-sitc2024.1377","is_oa":true,"landing_page_url":"https://doi.org/10.1136/jitc-2024-sitc2024.1377","pdf_url":null,"source":{"id":"https://openalex.org/S2754803477","display_name":"Journal for ImmunoTherapy of Cancer","issn_l":"2051-1426","issn":["2051-1426"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319945","host_organization_name":"BMJ","host_organization_lineage":["https://openalex.org/P4310319945"],"host_organization_lineage_names":["BMJ"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Regular and Young Investigator Award Abstracts","raw_type":"proceedings-article"}],"best_oa_location":{"id":"doi:10.1136/jitc-2024-sitc2024.1377","doi":"https://doi.org/10.1136/jitc-2024-sitc2024.1377","is_oa":true,"landing_page_url":"https://doi.org/10.1136/jitc-2024-sitc2024.1377","pdf_url":null,"source":{"id":"https://openalex.org/S2754803477","display_name":"Journal for ImmunoTherapy of Cancer","issn_l":"2051-1426","issn":["2051-1426"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319945","host_organization_name":"BMJ","host_organization_lineage":["https://openalex.org/P4310319945"],"host_organization_lineage_names":["BMJ"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Regular and Young Investigator Award Abstracts","raw_type":"proceedings-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9763000011444092,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4388068153","https://openalex.org/W4362596213","https://openalex.org/W4393088472","https://openalex.org/W4404063802","https://openalex.org/W4415898651","https://openalex.org/W4393087550","https://openalex.org/W4404064525","https://openalex.org/W4404166129","https://openalex.org/W4385687033","https://openalex.org/W4308379974"],"abstract_inverted_index":{"Background":[0],"Brain":[1],"tumors":[2,204,354],"present":[3],"a":[4,18,111,151],"complex":[5,33],"and":[6,55,72,102,129,139,162,177,214,232,235,238,240,248,263,269,293,324,351,378],"highly":[7],"heterogeneous":[8],"form":[9],"of":[10,21,36,51,69,75,84,93,110,127,142,157,180,197,230,275,279,334,349,366],"cancer":[11],"that":[12],"is":[13,46],"challenging":[14],"to":[15,135,383],"treat,":[16],"with":[17],"median":[19],"survival":[20],"just":[22],"over":[23],"one":[24],"year":[25],"after":[26],"diagnosis":[27],"for":[28,48,392],"primary":[29],"glioblastomas.":[30],"Identifying":[31],"the":[32,37,76,91,108,137,143,148,181,194,219,227,338,347,356,367],"spatial":[34,195,332],"composition":[35],"brain":[38,117,144,159,339,353],"tumor":[39,160,268],"microenvironment":[40],"(TME)":[41],"remains":[42],"challenging,":[43],"yet":[44],"it":[45],"vital":[47],"accurate":[49],"interpretation":[50],"disease":[52],"origin,":[53],"progression":[54],"treatment.":[56],"Imaging":[57,184],"Mass":[58],"Cytometry":[59],"(IMC\u2122)":[60],"approach":[61,343],"address":[62],"these":[63,280],"challenges":[64],"by":[65,172,187],"enabling":[66],"quantitative":[67,188],"evaluation":[68,333],"multiparametric":[70],"structural":[71,140],"cellular":[73,138,335],"features":[74],"TME.":[77,145],"An":[78],"IMC":[79,113,123,342],"based":[80],"analysis":[81,286,297],"facilitates":[82],"exploration":[83],"tissue":[85,100,152,178,303,328],"heterogeneity":[86,336],"across":[87,218,246],"patient":[88,385],"samples":[89],"without":[90,355],"complications":[92],"multiplexed":[94],"fluorescence-based":[95,361],"approaches,":[96],"such":[97,206,258],"as":[98,207,259],"autofluorescence,":[99],"degradation":[101],"spectral":[103],"overlap.":[104],"Here,":[105],"we":[106],"showcase":[107],"application":[109],"neuro-oncology-themed":[112],"panel":[114,149],"on":[115,150,298],"various":[116],"neoplasms.":[118],"Methods":[119],"A":[120],"40-plus-marker":[121],"neuro-oncology":[122],"antibody":[124],"panel,":[125],"consisting":[126],"neurophenotyping":[128,223],"human":[130,158,203],"immuno-oncology":[131],"panels,":[132],"was":[133],"used":[134],"determine":[136],"landscapes":[141],"We":[146,192,225],"applied":[147],"microarray":[153],"(TMA)":[154],"containing":[155,305],"dozens":[156],"cores":[161],"performed":[163],"imaging":[164,362],"using":[165,222,284,341],"two":[166],"whole":[167],"slide":[168],"scanning":[169],"workflows":[170],"enabled":[171],"preview":[173],"mode,":[174],"cell":[175,199,244,290],"mode":[176,179],"Hyperion":[182],"XTi\u2122":[183],"System,":[185],"followed":[186],"data":[189,301],"analysis.":[190],"Results":[191],"mapped":[193],"location":[196],"brain-specific":[198],"populations":[200],"making":[201],"up":[202],"\u2013":[205,217],"NeuN+":[208],"neurons,":[209],"GFAP+":[210],"astrocytes,":[211,311],"Iba1+":[212],"microglia":[213],"Olig2+":[215],"oligodendrocytes":[216],"entire":[220],"TMA":[221],"markers.":[224],"classified":[226],"distinct":[228],"states":[229],"neurons":[231],"quantified":[233],"CD68+":[234],"CD163+":[236],"myeloid":[237],"CD8+":[239],"CD57+":[241],"lymphoid":[242],"immune":[243,271,315],"infiltration":[245],"astrocytoma":[247],"glioblastoma":[249],"tissues.":[250],"In":[251],"addition,":[252],"cells":[253,272,281],"expressing":[254],"clinically":[255],"relevant":[256,327],"biomarkers,":[257],"PD-L1,":[260],"HLA-DR,":[261],"CD66b":[262],"CD44,":[264],"were":[265,282],"detected":[266],"in":[267,273,337,360,372,394],"infiltrated":[270],"subsets":[274],"tumors.":[276],"Relative":[277],"quantities":[278],"measured":[283],"single-cell":[285],"utilizing":[287],"machine":[288],"learning-based":[289],"segmentation":[291],"tools":[292],"cell-clustering":[294],"algorithms.":[295],"Pixel-clustering":[296],"Tissue":[299],"Mode":[300],"segregated":[302],"compartments":[304],"glioma-initiating":[306],"cells,":[307,309,316],"stem":[308],"activated":[310],"cancer-associated":[312],"fibroblasts,":[313],"infiltrating":[314],"immune-tumor":[317],"interfaces,":[318],"microglia,":[319],"tumor-associated":[320],"macrophages,":[321],"angiogenic":[322],"vasculature":[323],"other":[325,352],"biologically":[326],"structures.":[329],"Conclusions":[330],"Quantitative":[331],"TME":[340],"provides":[344],"insights":[345],"into":[346],"complexity":[348],"gliomas":[350],"artifacts":[357],"commonly":[358],"seen":[359],"techniques.":[363],"In-depth":[364],"deciphering":[365],"glioma":[368],"landscape":[369],"can":[370],"help":[371],"guiding":[373],"potential":[374],"personalized":[375],"therapeutic":[376],"strategies":[377],"effective":[379],"future":[380],"clinical":[381],"solutions":[382],"improve":[384],"outcomes.":[386],"For":[387],"Research":[388],"Use":[389],"Only.":[390],"Not":[391],"use":[393],"diagnostic":[395],"procedures.":[396]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2024-11-06T00:00:00"}