{"id":"https://openalex.org/W4403711166","doi":"https://doi.org/10.1101/2024.10.21.619323","title":"Super Resolved Single-Cell Spatial Metabolomics from Multimodal Mass Spectrometry Imaging guided by Imaging Mass Cytometry","display_name":"Super Resolved Single-Cell Spatial Metabolomics from Multimodal Mass Spectrometry Imaging guided by Imaging Mass Cytometry","publication_year":2024,"publication_date":"2024-10-24","ids":{"openalex":"https://openalex.org/W4403711166","doi":"https://doi.org/10.1101/2024.10.21.619323","pmid":"https://pubmed.ncbi.nlm.nih.gov/39484548","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/11526965"},"language":"en","primary_location":{"id":"doi:10.1101/2024.10.21.619323","doi":"https://doi.org/10.1101/2024.10.21.619323","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2024.10.21.619323","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.1101/2024.10.21.619323","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102929077","display_name":"Efe Ozturk","orcid":"https://orcid.org/0009-0002-9131-2677","observed_orcids":["https://orcid.org/0009-0002-9131-2677"]},"institutions":[{"id":"https://openalex.org/I130701444","display_name":"Georgia Institute of Technology","ror":"https://ror.org/01zkghx44","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444"]},{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]},{"id":"https://openalex.org/I2802612298","display_name":"The Wallace H. Coulter Department of Biomedical Engineering","ror":"https://ror.org/02j15s898","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444","https://openalex.org/I150468666","https://openalex.org/I2802612298"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Efe Ozturk","raw_affiliation_strings":["School of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, GA, USA 30332","Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA"],"raw_orcid":"https://orcid.org/0009-0002-9131-2677","affiliations":[{"raw_affiliation_string":"School of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, GA, USA 30332","institution_ids":["https://openalex.org/I130701444"]},{"raw_affiliation_string":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA","institution_ids":["https://openalex.org/I130701444","https://openalex.org/I150468666","https://openalex.org/I2802612298"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5114388766","display_name":"Abhijeet Venkataraman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I130701444","display_name":"Georgia Institute of Technology","ror":"https://ror.org/01zkghx44","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444"]},{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]},{"id":"https://openalex.org/I2802612298","display_name":"The Wallace H. Coulter Department of Biomedical Engineering","ror":"https://ror.org/02j15s898","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444","https://openalex.org/I150468666","https://openalex.org/I2802612298"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Abhijeet Venkataraman","raw_affiliation_strings":["Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA","institution_ids":["https://openalex.org/I130701444","https://openalex.org/I150468666","https://openalex.org/I2802612298"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5094354480","display_name":"Felix G. Rivera Moctezuma","orcid":"https://orcid.org/0009-0008-3431-7256","observed_orcids":["https://orcid.org/0009-0008-3431-7256"]},"institutions":[{"id":"https://openalex.org/I130701444","display_name":"Georgia Institute of Technology","ror":"https://ror.org/01zkghx44","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444"]},{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]},{"id":"https://openalex.org/I2802612298","display_name":"The Wallace H. Coulter Department of Biomedical Engineering","ror":"https://ror.org/02j15s898","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444","https://openalex.org/I150468666","https://openalex.org/I2802612298"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Felix G. Rivera Moctezuma","raw_affiliation_strings":["Interdisciplinary Bioengineering Graduate Program, Georgia Institute of Technology, Atlanta, GA, USA","Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA","Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Interdisciplinary Bioengineering Graduate Program, Georgia Institute of Technology, Atlanta, GA, USA","institution_ids":["https://openalex.org/I130701444"]},{"raw_affiliation_string":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA","institution_ids":["https://openalex.org/I130701444","https://openalex.org/I150468666","https://openalex.org/I2802612298"]},{"raw_affiliation_string":"Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA","institution_ids":["https://openalex.org/I130701444"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5044033293","display_name":"Ahmet F. Coskun","orcid":"https://orcid.org/0000-0002-5797-1524","observed_orcids":["https://orcid.org/0000-0002-5797-1524"]},"institutions":[{"id":"https://openalex.org/I130701444","display_name":"Georgia Institute of Technology","ror":"https://ror.org/01zkghx44","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444"]},{"id":"https://openalex.org/I150468666","display_name":"Emory University","ror":"https://ror.org/03czfpz43","country_code":"US","type":"education","lineage":["https://openalex.org/I150468666"]},{"id":"https://openalex.org/I2802612298","display_name":"The Wallace H. Coulter Department of Biomedical Engineering","ror":"https://ror.org/02j15s898","country_code":"US","type":"education","lineage":["https://openalex.org/I130701444","https://openalex.org/I150468666","https://openalex.org/I2802612298"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Ahmet F. Coskun","raw_affiliation_strings":["Interdisciplinary Bioengineering Graduate Program, Georgia Institute of Technology, Atlanta, GA, USA","Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA","Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA"],"raw_orcid":"https://orcid.org/0000-0002-5797-1524","affiliations":[{"raw_affiliation_string":"Interdisciplinary Bioengineering Graduate Program, Georgia Institute of Technology, Atlanta, GA, USA","institution_ids":["https://openalex.org/I130701444"]},{"raw_affiliation_string":"Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA","institution_ids":["https://openalex.org/I130701444"]},{"raw_affiliation_string":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA","institution_ids":["https://openalex.org/I130701444","https://openalex.org/I150468666","https://openalex.org/I2802612298"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5044033293"],"corresponding_institution_ids":["https://openalex.org/I130701444","https://openalex.org/I150468666","https://openalex.org/I2802612298"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9458000063896179,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10683","display_name":"Mass Spectrometry Techniques and Applications","score":0.9458000063896179,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10836","display_name":"Metabolomics and Mass Spectrometry Studies","score":0.0406000018119812,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.002300000051036477,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/super-resolution-imaging","display_name":"super-resolution imaging","score":0.6309999823570251},{"id":"https://openalex.org/keywords/mass-spectrometry-imaging","display_name":"mass spectrometry imaging","score":0.8939999938011169},{"id":"https://openalex.org/keywords/imaging-mass-cytometry","display_name":"imaging mass cytometry","score":0.8870000243186951},{"id":"https://openalex.org/keywords/multimodal-fusion","display_name":"multimodal fusion","score":0.675000011920929},{"id":"https://openalex.org/keywords/metabolite-mapping","display_name":"metabolite mapping","score":0.6430000066757202},{"id":"https://openalex.org/keywords/tissue-architecture","display_name":"tissue structure","score":0.7129999995231628}],"concepts":[{"id":"https://openalex.org/C24066741","wikidata":"https://www.wikidata.org/wiki/Q3796564","display_name":"Mass spectrometry imaging","level":3,"score":0.8289051055908203},{"id":"https://openalex.org/C2778015335","wikidata":"https://www.wikidata.org/wiki/Q17051163","display_name":"Mass cytometry","level":4,"score":0.8239554166793823},{"id":"https://openalex.org/C21565614","wikidata":"https://www.wikidata.org/wiki/Q12149006","display_name":"Metabolomics","level":2,"score":0.7096105813980103},{"id":"https://openalex.org/C162356407","wikidata":"https://www.wikidata.org/wiki/Q180809","display_name":"Mass spectrometry","level":2,"score":0.6263882517814636},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.37842726707458496},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.19647985696792603},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.0683819055557251},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.1101/2024.10.21.619323","doi":"https://doi.org/10.1101/2024.10.21.619323","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2024.10.21.619323","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmid:39484548","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/39484548","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"bioRxiv : the preprint server for biology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:11526965","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11526965","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1101/2024.10.21.619323","doi":"https://doi.org/10.1101/2024.10.21.619323","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2024.10.21.619323","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G6867947412","display_name":"Decoding Spatially Resolved Single Cell Metabolic Trajectory of Tonsil Tissues and Organoids","funder_award_id":"R21AI173900","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G6983410382","display_name":"Tissue systems biology of immune dysregulation in aging by single cell spatial metabolomics","funder_award_id":"R21AG081715","funder_id":"https://openalex.org/F4320337337","funder_display_name":"National Institute on Aging"},{"id":"https://openalex.org/G9000914292","display_name":"Dissecting subcellular and cellular organization by spatial molecular neighborhood networks","funder_award_id":"R35GM151028","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337337","display_name":"National Institute on Aging","ror":"https://ror.org/049v75w11"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":25,"referenced_works":["https://openalex.org/W1965011257","https://openalex.org/W1975292183","https://openalex.org/W1975567207","https://openalex.org/W1976648974","https://openalex.org/W2039946906","https://openalex.org/W2044712639","https://openalex.org/W2053129129","https://openalex.org/W2146299979","https://openalex.org/W2415311295","https://openalex.org/W2585179645","https://openalex.org/W2917807569","https://openalex.org/W2968947484","https://openalex.org/W2980705124","https://openalex.org/W3183905093","https://openalex.org/W3214596602","https://openalex.org/W4220772488","https://openalex.org/W4312524114","https://openalex.org/W4321222415","https://openalex.org/W4377115859","https://openalex.org/W4380202397","https://openalex.org/W4381612391","https://openalex.org/W4386076521","https://openalex.org/W4386137920","https://openalex.org/W4389684083","https://openalex.org/W4402005942"],"related_works":["https://openalex.org/W4416815317","https://openalex.org/W3150970936","https://openalex.org/W4313251991","https://openalex.org/W2730124516","https://openalex.org/W4401536076","https://openalex.org/W4414692686","https://openalex.org/W7167066356","https://openalex.org/W4415717216","https://openalex.org/W4402073312","https://openalex.org/W2571545805"],"abstract_inverted_index":{"Mass":[0,52],"spectrometry":[1],"imaging":[2],"(MSI)":[3],"is":[4],"a":[5,43],"powerful":[6],"technique":[7],"for":[8],"spatially":[9],"resolved":[10],"analysis":[11,89],"of":[12,26,61,77,90],"metabolites":[13],"and":[14,93],"other":[15],"biomolecules":[16],"within":[17],"biological":[18],"titissues.":[19],"However,":[20],"the":[21,58,75,104],"inherent":[22],"low":[23],"spatial":[24,59,101],"resolution":[25,60,76],"MSI":[27,63,78],"often":[28],"limits":[29],"its":[30],"ability":[31],"to":[32,56],"provide":[33],"detailed":[34,68],"cellular-level":[35],"information.":[36],"To":[37],"address":[38],"this":[39],"limitation,":[40],"we":[41,73],"propose":[42],"guided":[44],"super-resolution":[45],"(GSR)":[46],"approach":[47],"that":[48],"leverages":[49],"high-resolution":[50,81],"Imaging":[51],"Cytometry":[53],"(IMC)":[54],"images":[55,70],"enhance":[57],"low-resolution":[62],"data.":[64],"By":[65],"using":[66],"these":[67],"IMC":[69],"as":[71],"guides,":[72],"improve":[74],"images,":[79],"creang":[80],"metabolite":[82],"maps.":[83],"This":[84],"enhancement":[85],"facilitates":[86],"more":[87],"precise":[88],"cellular":[91],"structures":[92],"tissue":[94],"architectures,":[95],"providing":[96],"deeper":[97],"insights":[98],"into":[99],"super-resolved":[100],"metabolomics":[102],"at":[103],"single-cell":[105],"level.":[106]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1}],"updated_date":"2026-10-28T10:33:24.094632","created_date":"2024-10-25T00:00:00"}