{"id":"https://openalex.org/W4403560730","doi":"https://doi.org/10.17504/protocols.io.j8nlkok26v5r/v1","title":"Mouse Olfactory Horizontal Basal Cell Culture v1","display_name":"Mouse Olfactory Horizontal Basal Cell Culture v1","publication_year":2023,"publication_date":"2023-07-27","ids":{"openalex":"https://openalex.org/W4403560730","doi":"https://doi.org/10.17504/protocols.io.j8nlkok26v5r/v1"},"language":"en","primary_location":{"id":"doi:10.17504/protocols.io.j8nlkok26v5r/v1","doi":"https://doi.org/10.17504/protocols.io.j8nlkok26v5r/v1","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.j8nlkok26v5r/v1","pdf_url":"https://www.protocols.io/view/mouse-olfactory-horizontal-basal-cell-culture-cxt5xnq6.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.protocols.io/view/mouse-olfactory-horizontal-basal-cell-culture-cxt5xnq6.pdf","any_repository_has_fulltext":null},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5081762891","display_name":"Alp \u00d6zg\u00fcn","orcid":"https://orcid.org/0000-0003-4815-5098","observed_orcids":["https://orcid.org/0000-0003-4815-5098"]},"institutions":[{"id":"https://openalex.org/I4388482656","display_name":"Ottawa Hospital Research Institute","ror":"https://ror.org/05jtef216","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I153718931","https://openalex.org/I2800722420","https://openalex.org/I4388482656"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Alp Ozgun","raw_affiliation_strings":["Ottawa Hospital Research Institute Alp Ozgun Ottawa Hospital Research Institute"],"raw_orcid":"https://orcid.org/0000-0003-4815-5098","affiliations":[{"raw_affiliation_string":"Ottawa Hospital Research Institute Alp Ozgun Ottawa Hospital Research Institute","institution_ids":["https://openalex.org/I4388482656"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Priya Suman","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4388482656","display_name":"Ottawa Hospital Research Institute","ror":"https://ror.org/05jtef216","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I153718931","https://openalex.org/I2800722420","https://openalex.org/I4388482656"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Priya Suman","raw_affiliation_strings":["Ottawa Hospital Research Institute Alp Ozgun Ottawa Hospital Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ottawa Hospital Research Institute Alp Ozgun Ottawa Hospital Research Institute","institution_ids":["https://openalex.org/I4388482656"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Jos\u00e9e Coulombe","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4388482656","display_name":"Ottawa Hospital Research Institute","ror":"https://ror.org/05jtef216","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I153718931","https://openalex.org/I2800722420","https://openalex.org/I4388482656"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Jos\u00e9e Coulombe","raw_affiliation_strings":["Ottawa Hospital Research Institute Alp Ozgun Ottawa Hospital Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ottawa Hospital Research Institute Alp Ozgun Ottawa Hospital Research Institute","institution_ids":["https://openalex.org/I4388482656"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Julianna Tomlinson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4388482656","display_name":"Ottawa Hospital Research Institute","ror":"https://ror.org/05jtef216","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I153718931","https://openalex.org/I2800722420","https://openalex.org/I4388482656"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Julianna Tomlinson","raw_affiliation_strings":["Ottawa Hospital Research Institute Alp Ozgun Ottawa Hospital Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ottawa Hospital Research Institute Alp Ozgun Ottawa Hospital Research Institute","institution_ids":["https://openalex.org/I4388482656"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Earl G. Brown","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4388482656","display_name":"Ottawa Hospital Research Institute","ror":"https://ror.org/05jtef216","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I153718931","https://openalex.org/I2800722420","https://openalex.org/I4388482656"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Earl G. Brown","raw_affiliation_strings":["Ottawa Hospital Research Institute Alp Ozgun Ottawa Hospital Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ottawa Hospital Research Institute Alp Ozgun Ottawa Hospital Research Institute","institution_ids":["https://openalex.org/I4388482656"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090917194","display_name":"John Woulfe","orcid":"https://orcid.org/0000-0002-7382-6343","observed_orcids":["https://orcid.org/0000-0002-7382-6343","https://orcid.org/0000-0001-5061-3460"]},"institutions":[{"id":"https://openalex.org/I4388482656","display_name":"Ottawa Hospital Research Institute","ror":"https://ror.org/05jtef216","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I153718931","https://openalex.org/I2800722420","https://openalex.org/I4388482656"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"John M. Woulfe","raw_affiliation_strings":["Ottawa Hospital Research Institute Alp Ozgun Ottawa Hospital Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ottawa Hospital Research Institute Alp Ozgun Ottawa Hospital Research Institute","institution_ids":["https://openalex.org/I4388482656"]}]},{"author_position":"last","author":{"id":null,"display_name":"Michael G. Schlossmacher","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4388482656","display_name":"Ottawa Hospital Research Institute","ror":"https://ror.org/05jtef216","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I153718931","https://openalex.org/I2800722420","https://openalex.org/I4388482656"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"Michael G. Schlossmacher","raw_affiliation_strings":["Ottawa Hospital Research Institute Alp Ozgun Ottawa Hospital Research Institute"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Ottawa Hospital Research Institute Alp Ozgun Ottawa Hospital Research Institute","institution_ids":["https://openalex.org/I4388482656"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4388482656"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10971","display_name":"Olfactory and Sensory Function Studies","score":0.9580000042915344,"subfield":{"id":"https://openalex.org/subfields/2809","display_name":"Sensory Systems"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10971","display_name":"Olfactory and Sensory Function Studies","score":0.9580000042915344,"subfield":{"id":"https://openalex.org/subfields/2809","display_name":"Sensory Systems"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10608","display_name":"Neurogenesis and neuroplasticity mechanisms","score":0.016100000590085983,"subfield":{"id":"https://openalex.org/subfields/2806","display_name":"Developmental Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11204","display_name":"Ion Channels and Receptors","score":0.0026000000070780516,"subfield":{"id":"https://openalex.org/subfields/2809","display_name":"Sensory Systems"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/olfactory-epithelium","display_name":"olfactory epithelium","score":0.9810000061988831},{"id":"https://openalex.org/keywords/horizontal-basal-cells","display_name":"horizontal basal cells","score":0.8629999756813049},{"id":"https://openalex.org/keywords/cell-isolation","display_name":"cell isolation","score":0.6349999904632568},{"id":"https://openalex.org/keywords/cell-culture","display_name":"cell culture","score":0.7419999837875366},{"id":"https://openalex.org/keywords/enzymatic-digestion","display_name":"enzymatic digestion","score":0.7319999933242798},{"id":"https://openalex.org/keywords/keratin-5","display_name":"keratin 5","score":0.8399999737739563},{"id":"https://openalex.org/keywords/keratin-14","display_name":"keratin 14","score":0.9679999947547913},{"id":"https://openalex.org/keywords/mice","display_name":"mice","score":0.6620000004768372}],"concepts":[{"id":"https://openalex.org/C2778024521","wikidata":"https://www.wikidata.org/wiki/Q55639806","display_name":"Basal (medicine)","level":3,"score":0.5174701809883118},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4136675298213959},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.3637901544570923},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.08239573240280151},{"id":"https://openalex.org/C2779306644","wikidata":"https://www.wikidata.org/wiki/Q2002370","display_name":"Insulin","level":2,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.17504/protocols.io.j8nlkok26v5r/v1","doi":"https://doi.org/10.17504/protocols.io.j8nlkok26v5r/v1","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.j8nlkok26v5r/v1","pdf_url":"https://www.protocols.io/view/mouse-olfactory-horizontal-basal-cell-culture-cxt5xnq6.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.17504/protocols.io.j8nlkok26v5r/v1","doi":"https://doi.org/10.17504/protocols.io.j8nlkok26v5r/v1","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.j8nlkok26v5r/v1","pdf_url":"https://www.protocols.io/view/mouse-olfactory-horizontal-basal-cell-culture-cxt5xnq6.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.4043000042438507,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4403560730.pdf","grobid_xml":"https://content.openalex.org/works/W4403560730.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2085881147","https://openalex.org/W4234758982","https://openalex.org/W2378285691","https://openalex.org/W6926858883","https://openalex.org/W2092986522","https://openalex.org/W1588240583","https://openalex.org/W7120671017","https://openalex.org/W2050639384","https://openalex.org/W1983524092","https://openalex.org/W950387558"],"abstract_inverted_index":{"This":[0],"protocol":[1],"describes":[2],"isolation":[3],"and":[4,25,46],"culture":[5],"of":[6,13,21],"basal":[7,42],"cells":[8],"from":[9],"the":[10,40],"olfactory":[11,23],"epithelium":[12],"mice.":[14],"The":[15,36],"process":[16],"involves":[17],"serial":[18],"enzymatic":[19],"treatment":[20],"dissected":[22],"epithelia":[24],"subsequent":[26],"plating":[27],"in":[28],"commercial":[29],"airway":[30],"medium":[31],"with":[32],"dual":[33],"SMAD":[34],"inhibitors.":[35],"resulting":[37],"cultures":[38],"express":[39],"horizontal":[41],"cell":[43],"markers":[44],"CK5":[45],"CK14.":[47]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2024-10-19T00:00:00"}