{"id":"https://openalex.org/W4403258403","doi":"https://doi.org/10.1016/j.xpro.2024.103378","title":"Spatial analysis of transcript and protein levels in skeletal muscle","display_name":"Spatial analysis of transcript and protein levels in skeletal muscle","publication_year":2024,"publication_date":"2024-10-09","ids":{"openalex":"https://openalex.org/W4403258403","doi":"https://doi.org/10.1016/j.xpro.2024.103378","pmid":"https://pubmed.ncbi.nlm.nih.gov/39388355","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/11736001"},"language":"en","primary_location":{"id":"doi:10.1016/j.xpro.2024.103378","doi":"https://doi.org/10.1016/j.xpro.2024.103378","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xpro.2024.103378","pdf_url":null,"source":{"id":"https://openalex.org/S4210212477","display_name":"STAR Protocols","issn_l":"2666-1667","issn":["2666-1667"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protocols","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1016/j.xpro.2024.103378","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5081643111","display_name":"Paola Pisterzi","orcid":"https://orcid.org/0000-0001-6478-771X","observed_orcids":["https://orcid.org/0000-0001-6478-771X"]},"institutions":[{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"funder","lineage":["https://openalex.org/I2800006345"]},{"id":"https://openalex.org/I4405265031","display_name":"Novo Nordisk Foundation Center for Stem Cell Medicine","ror":"https://ror.org/03nqjyg83","country_code":"DK","type":"education","lineage":["https://openalex.org/I2801134892","https://openalex.org/I4405265031"]}],"countries":["DK","NL"],"is_corresponding":false,"raw_author_name":"Paola Pisterzi","raw_affiliation_strings":["Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands","institution_ids":["https://openalex.org/I2800006345","https://openalex.org/I4405265031"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078230591","display_name":"Clara Mart\u00ednez Mir","orcid":"https://orcid.org/0000-0002-5249-4976","observed_orcids":["https://orcid.org/0000-0002-5249-4976"]},"institutions":[{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"funder","lineage":["https://openalex.org/I2800006345"]},{"id":"https://openalex.org/I4405265031","display_name":"Novo Nordisk Foundation Center for Stem Cell Medicine","ror":"https://ror.org/03nqjyg83","country_code":"DK","type":"education","lineage":["https://openalex.org/I2801134892","https://openalex.org/I4405265031"]}],"countries":["DK","NL"],"is_corresponding":false,"raw_author_name":"Clara Martinez Mir","raw_affiliation_strings":["Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands","institution_ids":["https://openalex.org/I2800006345","https://openalex.org/I4405265031"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044585630","display_name":"Ouafa Dahri","orcid":"https://orcid.org/0000-0003-2170-2821","observed_orcids":["https://orcid.org/0000-0003-2170-2821"]},"institutions":[{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"funder","lineage":["https://openalex.org/I2800006345"]},{"id":"https://openalex.org/I4405265031","display_name":"Novo Nordisk Foundation Center for Stem Cell Medicine","ror":"https://ror.org/03nqjyg83","country_code":"DK","type":"education","lineage":["https://openalex.org/I2801134892","https://openalex.org/I4405265031"]}],"countries":["DK","NL"],"is_corresponding":false,"raw_author_name":"Ouafa Dahri","raw_affiliation_strings":["Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands","institution_ids":["https://openalex.org/I2800006345","https://openalex.org/I4405265031"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5107765958","display_name":"Isabel de Poorter","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"funder","lineage":["https://openalex.org/I2800006345"]},{"id":"https://openalex.org/I4405265031","display_name":"Novo Nordisk Foundation Center for Stem Cell Medicine","ror":"https://ror.org/03nqjyg83","country_code":"DK","type":"education","lineage":["https://openalex.org/I2801134892","https://openalex.org/I4405265031"]}],"countries":["DK","NL"],"is_corresponding":false,"raw_author_name":"Isabel de Poorter","raw_affiliation_strings":["Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands","institution_ids":["https://openalex.org/I2800006345","https://openalex.org/I4405265031"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108175501","display_name":"Sandra Batlles Parera","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"funder","lineage":["https://openalex.org/I2800006345"]},{"id":"https://openalex.org/I4405265031","display_name":"Novo Nordisk Foundation Center for Stem Cell Medicine","ror":"https://ror.org/03nqjyg83","country_code":"DK","type":"education","lineage":["https://openalex.org/I2801134892","https://openalex.org/I4405265031"]}],"countries":["DK","NL"],"is_corresponding":false,"raw_author_name":"Sandra Batlles Parera","raw_affiliation_strings":["Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands","institution_ids":["https://openalex.org/I2800006345","https://openalex.org/I4405265031"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075874189","display_name":"Milica Dostani\u0107","orcid":"https://orcid.org/0000-0002-3486-8487","observed_orcids":["https://orcid.org/0000-0002-3486-8487"]},"institutions":[{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"funder","lineage":["https://openalex.org/I2800006345"]},{"id":"https://openalex.org/I4405265031","display_name":"Novo Nordisk Foundation Center for Stem Cell Medicine","ror":"https://ror.org/03nqjyg83","country_code":"DK","type":"education","lineage":["https://openalex.org/I2801134892","https://openalex.org/I4405265031"]}],"countries":["DK","NL"],"is_corresponding":false,"raw_author_name":"Milica Dostani\u0107","raw_affiliation_strings":["Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands","institution_ids":["https://openalex.org/I2800006345","https://openalex.org/I4405265031"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073282606","display_name":"Massimo Mastrangeli","orcid":"https://orcid.org/0000-0003-3265-5768","observed_orcids":["https://orcid.org/0000-0003-3265-5768"]},"institutions":[{"id":"https://openalex.org/I98358874","display_name":"Delft University of Technology","ror":"https://ror.org/02e2c7k09","country_code":"NL","type":"education","lineage":["https://openalex.org/I98358874"]}],"countries":["NL"],"is_corresponding":false,"raw_author_name":"Massimo Mastrangeli","raw_affiliation_strings":["Microelectronics Department, Delft University of Technology, 2628 CD Delft, the Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Microelectronics Department, Delft University of Technology, 2628 CD Delft, the Netherlands","institution_ids":["https://openalex.org/I98358874"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085628953","display_name":"Christine L. Mummery","orcid":"https://orcid.org/0000-0002-4549-6535","observed_orcids":["https://orcid.org/0000-0002-4549-6535"]},"institutions":[{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"funder","lineage":["https://openalex.org/I2800006345"]},{"id":"https://openalex.org/I4405265031","display_name":"Novo Nordisk Foundation Center for Stem Cell Medicine","ror":"https://ror.org/03nqjyg83","country_code":"DK","type":"education","lineage":["https://openalex.org/I2801134892","https://openalex.org/I4405265031"]}],"countries":["DK","NL"],"is_corresponding":false,"raw_author_name":"Christine Mummery","raw_affiliation_strings":["Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands","institution_ids":["https://openalex.org/I2800006345","https://openalex.org/I4405265031"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072347898","display_name":"Niels Geijsen","orcid":"https://orcid.org/0000-0003-3592-4353","observed_orcids":["https://orcid.org/0000-0003-3592-4353"]},"institutions":[{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"funder","lineage":["https://openalex.org/I2800006345"]},{"id":"https://openalex.org/I4405265031","display_name":"Novo Nordisk Foundation Center for Stem Cell Medicine","ror":"https://ror.org/03nqjyg83","country_code":"DK","type":"education","lineage":["https://openalex.org/I2801134892","https://openalex.org/I4405265031"]}],"countries":["DK","NL"],"is_corresponding":true,"raw_author_name":"Niels Geijsen","raw_affiliation_strings":["Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands. Electronic address: n.geijsen@lumc.nl"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands. Electronic address: n.geijsen@lumc.nl","institution_ids":["https://openalex.org/I2800006345","https://openalex.org/I4405265031"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5067373629","display_name":"Fanny Sage","orcid":"https://orcid.org/0000-0002-3353-1647","observed_orcids":["https://orcid.org/0000-0002-3353-1647"]},"institutions":[{"id":"https://openalex.org/I2800006345","display_name":"Leiden University Medical Center","ror":"https://ror.org/05xvt9f17","country_code":"NL","type":"funder","lineage":["https://openalex.org/I2800006345"]},{"id":"https://openalex.org/I4405265031","display_name":"Novo Nordisk Foundation Center for Stem Cell Medicine","ror":"https://ror.org/03nqjyg83","country_code":"DK","type":"education","lineage":["https://openalex.org/I2801134892","https://openalex.org/I4405265031"]}],"countries":["DK","NL"],"is_corresponding":true,"raw_author_name":"Fanny Sage","raw_affiliation_strings":["Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands. Electronic address: f.g.sage@lumc.nl"],"raw_orcid":"https://orcid.org/0000-0002-3353-1647","affiliations":[{"raw_affiliation_string":"Department of Anatomy and Embryology, Leiden University Medical Center, 2333 Leiden, the Netherlands; The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden Node, the Netherlands. Electronic address: f.g.sage@lumc.nl","institution_ids":["https://openalex.org/I2800006345","https://openalex.org/I4405265031"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5067373629","https://openalex.org/A5072347898"],"corresponding_institution_ids":["https://openalex.org/I2800006345","https://openalex.org/I4405265031"],"apc_list":{"value":1500,"currency":"USD","value_usd":1500},"apc_paid":{"value":1500,"currency":"USD","value_usd":1500},"fwci":0.0,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":{"value":0.12294334,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"5","issue":"4","first_page":"103378","last_page":"103378"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10441","display_name":"Muscle Physiology and Disorders","score":0.7080000042915344,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10441","display_name":"Muscle Physiology and Disorders","score":0.7080000042915344,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.17030000686645508,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.013299999758601189,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/skeletal-muscle","display_name":"skeletal muscle","score":0.902999997138977},{"id":"https://openalex.org/keywords/spatial-transcriptomics","display_name":"spatial transcriptomics","score":0.8820000290870667},{"id":"https://openalex.org/keywords/spatial-proteomics","display_name":"spatial proteomics","score":0.8299999833106995},{"id":"https://openalex.org/keywords/tibialis-anterior","display_name":"tibialis anterior","score":0.878000020980835},{"id":"https://openalex.org/keywords/hybridization-chain-reaction","display_name":"hybridization chain reaction","score":0.949999988079071},{"id":"https://openalex.org/keywords/tissue-clearing","display_name":"tissue clearing","score":0.6729999780654907},{"id":"https://openalex.org/keywords/immunostaining","display_name":"immunostaining","score":0.8119999766349792},{"id":"https://openalex.org/keywords/formalin-fixation","display_name":"formalin fixation","score":0.7829999923706055},{"id":"https://openalex.org/keywords/confocal-microscopy","display_name":"confocal microscopy","score":0.7760000228881836}],"concepts":[{"id":"https://openalex.org/C2779959927","wikidata":"https://www.wikidata.org/wiki/Q1048687","display_name":"Skeletal muscle","level":2,"score":0.6723424196243286},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.45632222294807434},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.45229730010032654},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.37610965967178345},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.2949030101299286}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D018482","descriptor_name":"Muscle, Skeletal","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D062206","descriptor_name":"Spatial Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1016/j.xpro.2024.103378","doi":"https://doi.org/10.1016/j.xpro.2024.103378","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xpro.2024.103378","pdf_url":null,"source":{"id":"https://openalex.org/S4210212477","display_name":"STAR Protocols","issn_l":"2666-1667","issn":["2666-1667"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protocols","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:11736001","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11736001","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11736001/pdf/main.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protoc","raw_type":"Text"},{"id":"pmid:39388355","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/39388355","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR protocols","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:1ee48d89adb94b6b87659533164f4bf4","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/1ee48d89adb94b6b87659533164f4bf4","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"STAR Protocols, Vol 5, Iss 4, Pp 103378- (2024)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1016/j.xpro.2024.103378","doi":"https://doi.org/10.1016/j.xpro.2024.103378","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xpro.2024.103378","pdf_url":null,"source":{"id":"https://openalex.org/S4210212477","display_name":"STAR Protocols","issn_l":"2666-1667","issn":["2666-1667"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protocols","raw_type":"journal-article"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[{"score":0.6499999761581421,"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2898873767","display_name":"Netherlands Organ-on-Chip Initiative","funder_award_id":"024.003.001","funder_id":"https://openalex.org/F4320321800","funder_display_name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek"}],"funders":[{"id":"https://openalex.org/F4320320929","display_name":"Universiteit Leiden","ror":"https://ror.org/027bh9e22"},{"id":"https://openalex.org/F4320321800","display_name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","ror":"https://ror.org/04jsz6e67"},{"id":"https://openalex.org/F4320323080","display_name":"Leids Universitair Medisch Centrum","ror":"https://ror.org/05xvt9f17"},{"id":"https://openalex.org/F4320325957","display_name":"Novo Nordisk Fonden","ror":"https://ror.org/04txyc737"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":8,"referenced_works":["https://openalex.org/W1966955508","https://openalex.org/W2051650885","https://openalex.org/W2069314780","https://openalex.org/W2094844506","https://openalex.org/W2527833694","https://openalex.org/W2952471063","https://openalex.org/W4322731145","https://openalex.org/W4322731833"],"related_works":["https://openalex.org/W4403148189","https://openalex.org/W4402323442","https://openalex.org/W2973038855","https://openalex.org/W4301185253","https://openalex.org/W3165741501","https://openalex.org/W2384150820","https://openalex.org/W7097559141","https://openalex.org/W7161847712","https://openalex.org/W4392365712","https://openalex.org/W4393333416"],"abstract_inverted_index":{"Skeletal":[0,140],"muscle":[1,15,89,100,121,141,155],"spatial":[2,27,80,142,167],"analyses":[3,143],"have":[4,144],"revealed":[5,145],"unexpected":[6,146],"regionalized":[7,147],"gene":[8,148],"expression":[9,149],"patterns":[10,150],"challenging":[11,151],"the":[12,26,152,166],"understanding":[13,153],"of":[14,29,82,97,119,154,169],"as":[16,156],"a":[17,23,157,163],"homogeneous":[18,158],"tissue.":[19,159],"Here,":[20,160],"we":[21,161],"present":[22,162],"protocol":[24,69,78,128,164,209],"for":[25,41,62,79,92,103,113,135,165,181,202],"analysis":[28,81,168],"transcript":[30,170],"and":[31,50,56,66,84,109,116,138,171,190,196,206],"protein":[32,172],"levels":[33,173],"in":[34,86,174],"murine":[35,87,175],"skeletal":[36,176],"muscle.":[37,177],"We":[38,58,178,198],"describe":[39,179],"steps":[40,180],"tibialis":[42,182],"anterior":[43,183],"dissection,":[44,184],"formaldehyde":[45,185],"fixation,":[46,186],"tissue":[47,64,187,204],"chopper":[48,188],"cutting,":[49,189],"hybridization":[51,104,191],"chain":[52,105,192],"reaction":[53,106,193],"(HCR)":[54,107,194],"detection":[55,108,195],"amplification.":[57,197],"then":[59,199],"detail":[60,200],"procedures":[61,201],"immunostaining,":[63,114,203],"clearing,":[65,115,205],"imaging.":[67,207],"This":[68,208],"is":[70,210],"easily":[71,211],"adaptable":[72,212],"to":[73,131,213],"other":[74,214],"tissues.":[75,215],"\u2022":[76,90,101,111],"Detailed":[77],"transcripts":[83],"proteins":[85],"TA":[88,99],"Steps":[91,112],"obtaining":[93],"optimal":[94],"thick":[95,120],"sections":[96,122],"fixed":[98],"Guide":[102],"amplification":[110],"confocal":[117],"imaging":[118],"Publisher\u2019s":[123],"note:":[124],"Undertaking":[125],"any":[126],"experimental":[127],"requires":[129],"adherence":[130],"local":[132],"institutional":[133],"guidelines":[134],"laboratory":[136],"safety":[137],"ethics.":[139]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2024-10-10T00:00:00"}