{"id":"https://openalex.org/W4402944843","doi":"https://doi.org/10.17504/protocols.io.kxygx33yog8j/v2","title":"Electrophysiology and 2-photon imaging of Ca+2-transients v2","display_name":"Electrophysiology and 2-photon imaging of Ca+2-transients v2","publication_year":2023,"publication_date":"2023-08-17","ids":{"openalex":"https://openalex.org/W4402944843","doi":"https://doi.org/10.17504/protocols.io.kxygx33yog8j/v2"},"language":"en","primary_location":{"id":"doi:10.17504/protocols.io.kxygx33yog8j/v2","doi":"https://doi.org/10.17504/protocols.io.kxygx33yog8j/v2","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.kxygx33yog8j/v2","pdf_url":"https://www.protocols.io/view/electrophysiology-and-2-photon-imaging-of-ca-2-tra-dh4538y6.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.protocols.io/view/electrophysiology-and-2-photon-imaging-of-ca-2-tra-dh4538y6.pdf","any_repository_has_fulltext":null},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5038735537","display_name":"Beatriz Elizabeth Nielsen","orcid":"https://orcid.org/0000-0001-9927-3639","observed_orcids":["https://orcid.org/0000-0001-9927-3639"]},"institutions":[{"id":"https://openalex.org/I51713134","display_name":"University of Colorado Anschutz","ror":"https://ror.org/03wmf1y16","country_code":"US","type":"education","lineage":["https://openalex.org/I51713134"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Beatriz E Nielsen","raw_affiliation_strings":["University of Colorado Anschutz Medical Campus"],"raw_orcid":"https://orcid.org/0000-0001-9927-3639","affiliations":[{"raw_affiliation_string":"University of Colorado Anschutz Medical Campus","institution_ids":["https://openalex.org/I51713134"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5069292888","display_name":"Andrew G. Yee","orcid":"https://orcid.org/0000-0002-0981-6634","observed_orcids":["https://orcid.org/0000-0002-0981-6634"]},"institutions":[{"id":"https://openalex.org/I51713134","display_name":"University of Colorado Anschutz","ror":"https://ror.org/03wmf1y16","country_code":"US","type":"education","lineage":["https://openalex.org/I51713134"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrew G Yee","raw_affiliation_strings":["University of Colorado Anschutz Medical Campus"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"University of Colorado Anschutz Medical Campus","institution_ids":["https://openalex.org/I51713134"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I51713134"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.4871000051498413,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10077","display_name":"Neuroscience and Neuropharmacology Research","score":0.4871000051498413,"subfield":{"id":"https://openalex.org/subfields/2804","display_name":"Cellular and Molecular Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.48579999804496765,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10581","display_name":"Neural dynamics and brain function","score":0.0035000001080334187,"subfield":{"id":"https://openalex.org/subfields/2805","display_name":"Cognitive Neuroscience"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dendritic-calcium-transients","display_name":"dendritic calcium transients","score":0.9940000176429749},{"id":"https://openalex.org/keywords/backpropagating-action-potentials","display_name":"backpropagating action potentials","score":0.9649999737739563},{"id":"https://openalex.org/keywords/two-photon-calcium-imaging","display_name":"two-photon calcium imaging","score":0.8050000071525574},{"id":"https://openalex.org/keywords/dendritic-calcium-signaling","display_name":"dendritic calcium signaling","score":0.6100000143051147},{"id":"https://openalex.org/keywords/neuronal-electrophysiology","display_name":"neuronal electrophysiology","score":0.675000011920929},{"id":"https://openalex.org/keywords/dendritic-excitability","display_name":"dendritic excitability","score":0.40299999713897705}],"concepts":[{"id":"https://openalex.org/C185263204","wikidata":"https://www.wikidata.org/wiki/Q1154774","display_name":"Electrophysiology","level":2,"score":0.7071958184242249},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.46506884694099426},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.35129520297050476},{"id":"https://openalex.org/C169760540","wikidata":"https://www.wikidata.org/wiki/Q207011","display_name":"Neuroscience","level":1,"score":0.3149562478065491},{"id":"https://openalex.org/C15744967","wikidata":"https://www.wikidata.org/wiki/Q9418","display_name":"Psychology","level":0,"score":0.25614601373672485}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.17504/protocols.io.kxygx33yog8j/v2","doi":"https://doi.org/10.17504/protocols.io.kxygx33yog8j/v2","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.kxygx33yog8j/v2","pdf_url":"https://www.protocols.io/view/electrophysiology-and-2-photon-imaging-of-ca-2-tra-dh4538y6.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.17504/protocols.io.kxygx33yog8j/v2","doi":"https://doi.org/10.17504/protocols.io.kxygx33yog8j/v2","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.kxygx33yog8j/v2","pdf_url":"https://www.protocols.io/view/electrophysiology-and-2-photon-imaging-of-ca-2-tra-dh4538y6.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"sustainable_development_goals":[],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4402944843.pdf","grobid_xml":"https://content.openalex.org/works/W4402944843.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4405660913","https://openalex.org/W4200009014","https://openalex.org/W4412794377","https://openalex.org/W2983843870","https://openalex.org/W1617602615","https://openalex.org/W2495310975","https://openalex.org/W2794570232","https://openalex.org/W6944225510","https://openalex.org/W2023175942","https://openalex.org/W6906738204"],"abstract_inverted_index":{"This":[0],"protocol":[1],"describes":[2],"the":[3,104],"steps":[4],"for":[5],"imaging":[6,16],"dendritic":[7],"calcium":[8],"transients":[9],"evoked":[10],"by":[11,112],"backprogating":[12],"action":[13],"potentials.":[14],"2-photon":[15,21],"was":[17,39,62,83,100,109],"performed":[18],"using":[19,50,85],"a":[20,28,51,65,70,86,113],"laser":[22,34],"scanning":[23],"microscopy":[24],"system,":[25],"custom-built":[26],"on":[27],"BX51WI":[29],"microscope":[30],"(Olympus).":[31],"A":[32,93],"Ti:Sapphire":[33],"(Chameleon":[35],"Ultra":[36],"I;":[37],"Coherent)":[38],"tuned":[40],"to":[41,102,106],"emit":[42],"pulsed":[43],"excitation":[44],"at":[45],"920":[46],"nm":[47],"and":[48,75,78,82],"scanned":[49],"pair":[52],"of":[53],"X-Y":[54],"galvanometer":[55],"mirrors":[56],"(6215,":[57],"Cambridge":[58],"Technology).":[59],"Emitted":[60],"fluorescence":[61],"collected":[63],"through":[64],"water-immersion":[66],"objective":[67],"(60X,":[68],"Olympus),":[69],"dichroic":[71],"mirror":[72],"(T700LPXXR,":[73],"Chroma)":[74],"filters":[76],"(ET680sp":[77],"ET525/50":[79],"m-2P,":[80],"Chroma),":[81],"detected":[84],"GaAsP":[87],"photomultiplier":[88],"tube":[89],"(PMT,":[90],"H10770PA-40,":[91],"Hamamatsu).":[92],"current":[94],"preamplifier":[95],"(SR570,":[96],"Stanford":[97],"Research":[98],"Systems)":[99],"used":[101],"convert":[103],"output":[105],"voltage,":[107],"which":[108],"then":[110],"digitized":[111],"data":[114],"acquisition":[115],"card":[116],"(PCI-6110,":[117],"National":[118],"Instruments).":[119]},"counts_by_year":[],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2024-09-28T00:00:00"}