{"id":"https://openalex.org/W4402559615","doi":"https://doi.org/10.20944/preprints202409.1114.v1","title":"Assessing the Pathogenicity of Berkeleyomyces rouxiae and Fusarium oxysporum f. sp. vasinfectum on Cotton (Gossypium hirsutum) Using a Rapid and Robust Seedling Screening Method","display_name":"Assessing the Pathogenicity of Berkeleyomyces rouxiae and Fusarium oxysporum f. sp. vasinfectum on Cotton (Gossypium hirsutum) Using a Rapid and Robust Seedling Screening Method","publication_year":2024,"publication_date":"2024-09-14","ids":{"openalex":"https://openalex.org/W4402559615","doi":"https://doi.org/10.20944/preprints202409.1114.v1"},"language":"en","primary_location":{"id":"doi:10.20944/preprints202409.1114.v1","doi":"https://doi.org/10.20944/preprints202409.1114.v1","is_oa":true,"landing_page_url":"http://dx.doi.org/10.20944/preprints202409.1114.v1","pdf_url":"https://www.preprints.org/frontend/manuscript/d622e175da09c945e6eb9d7e49ff8ea1/download_pub","source":{"id":"https://openalex.org/S6309402219","display_name":"Preprints.org","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.preprints.org/frontend/manuscript/d622e175da09c945e6eb9d7e49ff8ea1/download_pub","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5044024227","display_name":"Andrew Chen","orcid":"https://orcid.org/0000-0002-4004-1283","observed_orcids":["https://orcid.org/0000-0002-4004-1283"]},"institutions":[{"id":"https://openalex.org/I165143802","display_name":"The University of Queensland","ror":"https://ror.org/00rqy9422","country_code":"AU","type":"education","lineage":["https://openalex.org/I165143802"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Andrew Chen","raw_affiliation_strings":["School of Agriculture and Food Sustainability , The University of Queensland , St. Lucia , QLD 4072 , Australia"],"raw_orcid":"https://orcid.org/0000-0002-4004-1283","affiliations":[{"raw_affiliation_string":"School of Agriculture and Food Sustainability , The University of Queensland , St. Lucia , QLD 4072 , Australia","institution_ids":["https://openalex.org/I165143802"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017929584","display_name":"Duy Phu Le","orcid":"https://orcid.org/0000-0003-3331-1342","observed_orcids":["https://orcid.org/0000-0003-3331-1342"]},"institutions":[{"id":"https://openalex.org/I2801559191","display_name":"New South Wales Department of Primary Industries","ror":"https://ror.org/050khh066","country_code":"AU","type":"government","lineage":["https://openalex.org/I2801351115","https://openalex.org/I2801559191"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Duy P. Le","raw_affiliation_strings":["New South Wales Department of Primary Industries , Narrabri , NSW 2390 , Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"New South Wales Department of Primary Industries , Narrabri , NSW 2390 , Australia","institution_ids":["https://openalex.org/I2801559191"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103032414","display_name":"Linda J. Smith","orcid":"https://orcid.org/0000-0001-8629-9610","observed_orcids":["https://orcid.org/0000-0001-8629-9610"]},"institutions":[{"id":"https://openalex.org/I4210164589","display_name":"Department of Primary Industries","ror":"https://ror.org/05s5aag36","country_code":"AU","type":"government","lineage":["https://openalex.org/I2801244131","https://openalex.org/I4210164589"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Linda J. Smith","raw_affiliation_strings":["Ecosciences Precinct , Department of Agriculture and Fisheries , Dutton Park , QLD 4102 , Australia. L"],"raw_orcid":"https://orcid.org/0000-0001-8629-9610","affiliations":[{"raw_affiliation_string":"Ecosciences Precinct , Department of Agriculture and Fisheries , Dutton Park , QLD 4102 , Australia. L","institution_ids":["https://openalex.org/I4210164589"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005221859","display_name":"Dinesh Kafle","orcid":"https://orcid.org/0000-0002-1009-3867","observed_orcids":["https://orcid.org/0000-0002-1009-3867"]},"institutions":[{"id":"https://openalex.org/I4210164589","display_name":"Department of Primary Industries","ror":"https://ror.org/05s5aag36","country_code":"AU","type":"government","lineage":["https://openalex.org/I2801244131","https://openalex.org/I4210164589"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Dinesh Kafle","raw_affiliation_strings":["Ecosciences Precinct , Department of Agriculture and Fisheries , Dutton Park , QLD 4102 , Australia. L"],"raw_orcid":"https://orcid.org/0000-0002-1009-3867","affiliations":[{"raw_affiliation_string":"Ecosciences Precinct , Department of Agriculture and Fisheries , Dutton Park , QLD 4102 , Australia. L","institution_ids":["https://openalex.org/I4210164589"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035693370","display_name":"Elizabeth A. B. Aitken","orcid":"https://orcid.org/0000-0002-3792-4229","observed_orcids":["https://orcid.org/0000-0002-3792-4229"]},"institutions":[{"id":"https://openalex.org/I165143802","display_name":"The University of Queensland","ror":"https://ror.org/00rqy9422","country_code":"AU","type":"education","lineage":["https://openalex.org/I165143802"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Elizabeth A. B. Aitken","raw_affiliation_strings":["School of Agriculture and Food Sustainability , The University of Queensland , St. Lucia , QLD 4072 , Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Agriculture and Food Sustainability , The University of Queensland , St. Lucia , QLD 4072 , Australia","institution_ids":["https://openalex.org/I165143802"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5025428135","display_name":"Donald Max Gardiner","orcid":"https://orcid.org/0000-0002-2162-8716","observed_orcids":["https://orcid.org/0000-0002-2162-8716"]},"institutions":[{"id":"https://openalex.org/I165143802","display_name":"The University of Queensland","ror":"https://ror.org/00rqy9422","country_code":"AU","type":"education","lineage":["https://openalex.org/I165143802"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Donald M. Gardiner","raw_affiliation_strings":["Queensland Alliance for Agriculture and Food Innovation , The University of Queensland , St. Lucia , QLD 4072 , Australia"],"raw_orcid":"https://orcid.org/0000-0002-2162-8716","affiliations":[{"raw_affiliation_string":"Queensland Alliance for Agriculture and Food Innovation , The University of Queensland , St. Lucia , QLD 4072 , Australia","institution_ids":["https://openalex.org/I165143802"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10825","display_name":"Plant Pathogens and Fungal Diseases","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10825","display_name":"Plant Pathogens and Fungal Diseases","score":0.9911999702453613,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12660","display_name":"Plant Disease Management Techniques","score":0.0024999999441206455,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12858","display_name":"Plant Disease Resistance and Genetics","score":0.002400000113993883,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fusarium-oxysporum-f-sp-vasinfectum","display_name":"Fusarium oxysporum f. sp. vasinfectum","score":1.0},{"id":"https://openalex.org/keywords/fusarium-wilt","display_name":"Fusarium wilt","score":0.8090000152587891},{"id":"https://openalex.org/keywords/gossypium-hirsutum","display_name":"Gossypium hirsutum","score":0.9350000023841858},{"id":"https://openalex.org/keywords/pathogenicity","display_name":"pathogenicity","score":0.7789999842643738},{"id":"https://openalex.org/keywords/seedling-screening","display_name":"seedling screening","score":0.7149999737739563},{"id":"https://openalex.org/keywords/virulence","display_name":"virulence","score":0.7009999752044678},{"id":"https://openalex.org/keywords/soil-borne-pathogens","display_name":"soil-borne pathogens","score":0.7630000114440918},{"id":"https://openalex.org/keywords/root-colonization","display_name":"root colonization","score":0.5210000276565552},{"id":"https://openalex.org/keywords/endophytes","display_name":"endophytes","score":0.699999988079071},{"id":"https://openalex.org/keywords/plant-disease-severity","display_name":"plant disease severity","score":0.4180000126361847}],"concepts":[{"id":"https://openalex.org/C2779430813","wikidata":"https://www.wikidata.org/wiki/Q139958","display_name":"Fusarium oxysporum","level":2,"score":0.7295679450035095},{"id":"https://openalex.org/C64502627","wikidata":"https://www.wikidata.org/wiki/Q170065","display_name":"Pathogenicity","level":2,"score":0.6966266632080078},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4530477523803711},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.31520551443099976},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.2921685576438904}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.20944/preprints202409.1114.v1","doi":"https://doi.org/10.20944/preprints202409.1114.v1","is_oa":true,"landing_page_url":"http://dx.doi.org/10.20944/preprints202409.1114.v1","pdf_url":"https://www.preprints.org/frontend/manuscript/d622e175da09c945e6eb9d7e49ff8ea1/download_pub","source":{"id":"https://openalex.org/S6309402219","display_name":"Preprints.org","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.20944/preprints202409.1114.v1","doi":"https://doi.org/10.20944/preprints202409.1114.v1","is_oa":true,"landing_page_url":"http://dx.doi.org/10.20944/preprints202409.1114.v1","pdf_url":"https://www.preprints.org/frontend/manuscript/d622e175da09c945e6eb9d7e49ff8ea1/download_pub","source":{"id":"https://openalex.org/S6309402219","display_name":"Preprints.org","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"sustainable_development_goals":[{"score":0.991100013256073,"display_name":"Zero hunger","id":"https://metadata.un.org/sdg/2"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5140778754","display_name":null,"funder_award_id":"IH190100022","funder_id":"https://openalex.org/F4320315885","funder_display_name":"Australian Government"}],"funders":[{"id":"https://openalex.org/F4320315885","display_name":"Australian Government","ror":"https://ror.org/0314h5y94"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4402559615.pdf","grobid_xml":"https://content.openalex.org/works/W4402559615.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7125970993","https://openalex.org/W3127604261","https://openalex.org/W3152059879","https://openalex.org/W4295423351","https://openalex.org/W4382046751","https://openalex.org/W3016299083","https://openalex.org/W3091808691","https://openalex.org/W4311492964","https://openalex.org/W1944906342","https://openalex.org/W3129069740"],"abstract_inverted_index":{"Cotton":[0],"(Gossypium":[1],"spp.)":[2],"is":[3],"the":[4,49,66,70,100,127,146,166,186,189,217,237,257],"most":[5],"important":[6],"fibre":[7],"crop":[8],"worldwide.":[9],"Black":[10],"root":[11],"rot":[12],"and":[13,27,69,78,87,151,155,227,242,280,282],"Fusarium":[14,28,160,195,261],"wilt":[15,161,205,244,262],"are":[16],"two":[17,114],"major":[18],"diseases":[19],"of":[20,51,75,102,115,129,131,159,173,188,219,230,252,260],"cotton":[21,83,93,163,201,214,241,264],"caused":[22,38],"by":[23,39,110],"soil-borne":[24,40],"Berkeleyomyces":[25],"rouxiae":[26,77,106],"oxysporum":[29,196],"f.":[30],"sp.":[31],"vasinfectum":[32],"(Fov),":[33],"respectively.":[34],"Phenotyping":[35],"plant":[36,67,96,174,278],"symptoms":[37,157,206],"pathogens":[41,279],"has":[42],"always":[43],"been":[44],"a":[45,55],"challenge.":[46],"To":[47,184],"increase":[48],"uniformity":[50],"infection,":[52],"we":[53],"adapted":[54],"seedling":[56],"screening":[57],"method":[58],"that":[59,248],"directly":[60],"uses":[61],"liquid":[62],"cultures":[63],"to":[64,119,134,169,212,239,256,274,288],"inoculate":[65],"roots":[68],"soil.":[71],"Four":[72],"isolates":[73,107,130,140,150,167,198,235],"each":[74],"B.":[76,105],"Fov":[79,139,149,190],"were":[80,88,108,117,141,207],"collected":[81,199],"from":[82,200,267],"fields":[84],"in":[85,126,179,263],"Australia":[86],"characterised":[89],"for":[90,209],"virulence":[91,181,187],"on":[92,122,162,182],"under":[94],"controlled":[95],"growth":[97],"conditions.":[98],"While":[99],"identity":[101],"all":[103,232],"four":[104,138,192,233],"confirmed":[109],"multi-loci":[111],"sequencing,":[112],"only":[113],"them":[116],"found":[118],"be":[120,275,289],"pathogenic":[121],"cotton,":[123],"suggesting":[124],"variability":[125],"ability":[128,211,238],"this":[132,268],"species":[133],"cause":[135],"disease.":[136],"The":[137],"phylogenetically":[142],"clustered":[143],"together":[144],"with":[145],"other":[147],"Australian":[148],"displayed":[152],"both":[153],"external":[154],"internal":[156],"characteristic":[158,229],"plants.":[164],"Furthermore,":[165],"appeared":[168],"induce":[170,243],"varied":[171],"levels":[172],"disease":[175],"severity":[176],"indicating":[177],"differences":[178],"their":[180,210],"cotton.":[183],"contrast":[185],"isolates,":[191],"putatively":[193],"non-pathogenic":[194],"(Fo)":[197],"seedlings":[202],"exhibiting":[203],"atypical":[204],"assessed":[208],"colonise":[213,240],"host.":[215],"Despite":[216],"absence":[218],"Secreted":[220],"In":[221],"Xylem":[222],"genes":[223],"(SIX6,":[224],"SIX11,":[225],"SIX13":[226],"SIX14)":[228],"Fov,":[231],"Fo":[234,253],"retained":[236],"symptoms.":[245],"This":[246],"suggests":[247],"slightly":[249],"virulent":[250],"strains":[251],"may":[254],"contribute":[255],"overall":[258],"occurrence":[259],"fields.":[265],"Findings":[266],"study":[269],"will":[270],"allow":[271,283],"better":[272],"distinction":[273],"made":[276],"between":[277],"endophytes":[281],"fungal":[284],"effectors":[285],"underpinning":[286],"pathogenicity":[287],"explored.":[290]},"counts_by_year":[{"year":2024,"cited_by_count":1}],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2024-09-18T00:00:00"}