{"id":"https://openalex.org/W4402196483","doi":"https://doi.org/10.1016/j.vascn.2024.107549","title":"Comparative analysis of high-throughput RNA extraction kits in Na\u00efve Non-Human Primate (NHP) tissues for downstream applications utilizing Xeno Internal Positive Control (IPC)","display_name":"Comparative analysis of high-throughput RNA extraction kits in Na\u00efve Non-Human Primate (NHP) tissues for downstream applications utilizing Xeno Internal Positive Control (IPC)","publication_year":2024,"publication_date":"2024-09-01","ids":{"openalex":"https://openalex.org/W4402196483","doi":"https://doi.org/10.1016/j.vascn.2024.107549","pmid":"https://pubmed.ncbi.nlm.nih.gov/39236994"},"language":"en","primary_location":{"id":"doi:10.1016/j.vascn.2024.107549","doi":"https://doi.org/10.1016/j.vascn.2024.107549","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.vascn.2024.107549","pdf_url":null,"source":{"id":"https://openalex.org/S89571363","display_name":"Journal of Pharmacological and Toxicological Methods","issn_l":"1056-8719","issn":["1056-8719","1873-488X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Pharmacological and Toxicological Methods","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.2139/ssrn.4832106","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5087471019","display_name":"Ruwini D. Rajapaksha","orcid":"https://orcid.org/0000-0003-3411-1676","observed_orcids":["https://orcid.org/0000-0003-3411-1676"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Ruwini D. Rajapaksha","raw_affiliation_strings":["Lovelace Biomedical Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, NM 87108-5127, United States of America. Electronic address: rrajapaksha@lovelacebiomedical.org","Lovelace Biomedical Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, NM 87108-5127, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lovelace Biomedical Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, NM 87108-5127, United States of America. Electronic address: rrajapaksha@lovelacebiomedical.org","institution_ids":[]},{"raw_affiliation_string":"Lovelace Biomedical Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, NM 87108-5127, United States of America","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"Catherine Brooks","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Catherine Brooks","raw_affiliation_strings":["Lovelace Biomedical Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, NM 87108-5127, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lovelace Biomedical Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, NM 87108-5127, United States of America","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073552652","display_name":"Adriana Rascon","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Adriana Rascon","raw_affiliation_strings":["Lovelace Biomedical Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, NM 87108-5127, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lovelace Biomedical Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, NM 87108-5127, United States of America","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5098727927","display_name":"Adam Fadem","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Adam Fadem","raw_affiliation_strings":["Lovelace Biomedical Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, NM 87108-5127, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lovelace Biomedical Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, NM 87108-5127, United States of America","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"Ivy Nguyen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ivy Nguyen","raw_affiliation_strings":["Lovelace Biomedical Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, NM 87108-5127, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lovelace Biomedical Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, NM 87108-5127, United States of America","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5007070596","display_name":"Philip J. Kuehl","orcid":"https://orcid.org/0000-0002-7567-3002","observed_orcids":["https://orcid.org/0000-0002-7567-3002"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Philip J. Kuehl","raw_affiliation_strings":["Lovelace Biomedical Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, NM 87108-5127, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lovelace Biomedical Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, NM 87108-5127, United States of America","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5106967926","display_name":"John T. Farmer","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"John T. Farmer","raw_affiliation_strings":["Lovelace Biomedical Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, NM 87108-5127, United States of America"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lovelace Biomedical Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque, NM 87108-5127, United States of America","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5087471019"],"corresponding_institution_ids":[],"apc_list":{"value":3320,"currency":"USD","value_usd":3320},"apc_paid":null,"fwci":0.2713,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.46920304,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":94,"max":96},"biblio":{"volume":"129","issue":null,"first_page":"107549","last_page":"107549"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rna-extraction","display_name":"RNA extraction","score":0.8600000143051147},{"id":"https://openalex.org/keywords/extraction-efficiency","display_name":"extraction efficiency","score":0.7979999780654907},{"id":"https://openalex.org/keywords/rna-yield","display_name":"RNA yield","score":0.7139999866485596},{"id":"https://openalex.org/keywords/rna-purity","display_name":"RNA purity","score":0.9580000042915344},{"id":"https://openalex.org/keywords/gene-therapy","display_name":"gene therapy","score":0.36000001430511475}],"concepts":[{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.8348666429519653},{"id":"https://openalex.org/C112190416","wikidata":"https://www.wikidata.org/wiki/Q2124450","display_name":"RNA extraction","level":4,"score":0.8026493787765503},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.46253445744514465},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4506648778915405},{"id":"https://openalex.org/C4725764","wikidata":"https://www.wikidata.org/wiki/Q844704","display_name":"Extraction (chemistry)","level":2,"score":0.4302118718624115},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.421161413192749},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.40590471029281616},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3152760863304138},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1638985574245453},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.15100139379501343}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001921","descriptor_name":"Brain","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008099","descriptor_name":"Liver","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008168","descriptor_name":"Lung","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011933","descriptor_name":"Reagent Kits, Diagnostic","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":false},{"descriptor_ui":"D012313","descriptor_name":"RNA","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012313","descriptor_name":"RNA","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D013154","descriptor_name":"Spleen","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D057166","descriptor_name":"High-Throughput Screening Assays","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D060888","descriptor_name":"Real-Time Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/j.vascn.2024.107549","doi":"https://doi.org/10.1016/j.vascn.2024.107549","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.vascn.2024.107549","pdf_url":null,"source":{"id":"https://openalex.org/S89571363","display_name":"Journal of Pharmacological and Toxicological Methods","issn_l":"1056-8719","issn":["1056-8719","1873-488X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Pharmacological and Toxicological Methods","raw_type":"journal-article"},{"id":"doi:10.2139/ssrn.4832106","doi":"https://doi.org/10.2139/ssrn.4832106","is_oa":true,"landing_page_url":"https://doi.org/10.2139/ssrn.4832106","pdf_url":null,"source":{"id":"https://openalex.org/S4210172589","display_name":"SSRN Electronic Journal","issn_l":"1556-5068","issn":["1556-5068"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1318003438","host_organization_name":"RELX Group (Netherlands)","host_organization_lineage":["https://openalex.org/I1318003438"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},{"id":"pmid:39236994","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/39236994","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of pharmacological and toxicological methods","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.2139/ssrn.4832106","doi":"https://doi.org/10.2139/ssrn.4832106","is_oa":true,"landing_page_url":"https://doi.org/10.2139/ssrn.4832106","pdf_url":null,"source":{"id":"https://openalex.org/S4210172589","display_name":"SSRN Electronic Journal","issn_l":"1556-5068","issn":["1556-5068"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1318003438","host_organization_name":"RELX Group (Netherlands)","host_organization_lineage":["https://openalex.org/I1318003438"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":24,"referenced_works":["https://openalex.org/W84882231","https://openalex.org/W1930294030","https://openalex.org/W1985178609","https://openalex.org/W2023996602","https://openalex.org/W2024565798","https://openalex.org/W2039713661","https://openalex.org/W2061240424","https://openalex.org/W2108842440","https://openalex.org/W2109057179","https://openalex.org/W2123788109","https://openalex.org/W2149903385","https://openalex.org/W2168420558","https://openalex.org/W2171149266","https://openalex.org/W2312331183","https://openalex.org/W2735156098","https://openalex.org/W2890617931","https://openalex.org/W3016580011","https://openalex.org/W3100645455","https://openalex.org/W4280494337","https://openalex.org/W4327900255","https://openalex.org/W4386656220","https://openalex.org/W4386910019","https://openalex.org/W4391530061","https://openalex.org/W6822528696"],"related_works":["https://openalex.org/W4411682613","https://openalex.org/W2024565798","https://openalex.org/W3199700531","https://openalex.org/W4387440802","https://openalex.org/W2079902500","https://openalex.org/W7164040242","https://openalex.org/W3007932897","https://openalex.org/W4380090188","https://openalex.org/W7099881484","https://openalex.org/W4406090212"],"abstract_inverted_index":null,"counts_by_year":[{"year":2025,"cited_by_count":2}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2024-09-04T00:00:00"}