{"id":"https://openalex.org/W4402021981","doi":"https://doi.org/10.1101/2024.08.23.609368","title":"Pipeline for Assessing Tumor Immune Status Using Superplex Immunostaining and Spatial Immune Interaction Analysis","display_name":"Pipeline for Assessing Tumor Immune Status Using Superplex Immunostaining and Spatial Immune Interaction Analysis","publication_year":2024,"publication_date":"2024-08-26","ids":{"openalex":"https://openalex.org/W4402021981","doi":"https://doi.org/10.1101/2024.08.23.609368"},"language":"en","primary_location":{"id":"doi:10.1101/2024.08.23.609368","doi":"https://doi.org/10.1101/2024.08.23.609368","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2024.08.23.609368","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.1101/2024.08.23.609368","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5003696338","display_name":"Chaoxin Xiao","orcid":"https://orcid.org/0000-0002-0764-9748","observed_orcids":["https://orcid.org/0000-0002-0764-9748"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Chaoxin Xiao","raw_affiliation_strings":["State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109763922","display_name":"Ruihan Zhou","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ruihan Zhou","raw_affiliation_strings":["State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100362858","display_name":"Qin Chen","orcid":"https://orcid.org/0000-0002-0526-2005","observed_orcids":["https://orcid.org/0000-0002-0526-2005"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qin Chen","raw_affiliation_strings":["State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056574442","display_name":"Wanting Hou","orcid":"https://orcid.org/0009-0006-1215-1043","observed_orcids":["https://orcid.org/0009-0006-1215-1043"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wanting Hou","raw_affiliation_strings":["Division of Abdominal Tumor, Department of Medical Oncology, Cancer Center and State Key Laboratory of Biological Therapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Abdominal Tumor, Department of Medical Oncology, Cancer Center and State Key Laboratory of Biological Therapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089761"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Xiaoying Li","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xiaoying Li","raw_affiliation_strings":["Division of Abdominal Tumor, Department of Medical Oncology, Cancer Center and State Key Laboratory of Biological Therapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Abdominal Tumor, Department of Medical Oncology, Cancer Center and State Key Laboratory of Biological Therapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089761"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100418050","display_name":"Yulin Wang","orcid":"https://orcid.org/0000-0002-9976-5315","observed_orcids":["https://orcid.org/0000-0002-9976-5315"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yulin Wang","raw_affiliation_strings":["State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100396547","display_name":"Lu Liu","orcid":"https://orcid.org/0000-0003-2683-7215","observed_orcids":["https://orcid.org/0000-0003-2683-7215"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lu Liu","raw_affiliation_strings":["State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100379120","display_name":"Huanhuan Wang","orcid":"https://orcid.org/0000-0001-6935-0760","observed_orcids":["https://orcid.org/0000-0001-6935-0760"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Huanhuan Wang","raw_affiliation_strings":["State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101486724","display_name":"Xiaohong Yao","orcid":"https://orcid.org/0000-0002-4689-9141","observed_orcids":["https://orcid.org/0000-0002-4689-9141","https://orcid.org/0000-0002-4423-7072"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I4210089228","display_name":"West China Second University Hospital of Sichuan University","ror":"https://ror.org/00726et14","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089228"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xiaohong Yao","raw_affiliation_strings":["Department of Pathology, West China Second University Hospital, Sichuan University, Chengdu 610041, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, West China Second University Hospital, Sichuan University, Chengdu 610041, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089228"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015884016","display_name":"Tongtong Xu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Tongtong Xu","raw_affiliation_strings":["State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101106782","display_name":"Fujun Cao","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Fujun Cao","raw_affiliation_strings":["State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113356548","display_name":"Banglei Yin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Banglei Yin","raw_affiliation_strings":["State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062251781","display_name":"Ouying Yan","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Ouying Yan","raw_affiliation_strings":["Division of Abdominal Tumor, Department of Medical Oncology, Cancer Center and State Key Laboratory of Biological Therapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Abdominal Tumor, Department of Medical Oncology, Cancer Center and State Key Laboratory of Biological Therapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089761"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075969904","display_name":"Lili Jiang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lili Jiang","raw_affiliation_strings":["Department of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089761"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100391746","display_name":"Wei Wang","orcid":"https://orcid.org/0000-0001-7788-1895","observed_orcids":["https://orcid.org/0000-0001-7788-1895"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I4210089228","display_name":"West China Second University Hospital of Sichuan University","ror":"https://ror.org/00726et14","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089228"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Wei Wang","raw_affiliation_strings":["Department of Pathology, West China Second University Hospital, Sichuan University, Chengdu 610041, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pathology, West China Second University Hospital, Sichuan University, Chengdu 610041, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089228"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052964199","display_name":"Dan Cao","orcid":"https://orcid.org/0000-0002-6709-4932","observed_orcids":["https://orcid.org/0000-0002-6709-4932"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Dan Cao","raw_affiliation_strings":["Division of Abdominal Tumor, Department of Medical Oncology, Cancer Center and State Key Laboratory of Biological Therapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Abdominal Tumor, Department of Medical Oncology, Cancer Center and State Key Laboratory of Biological Therapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089761"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5034823522","display_name":"Chengjian Zhao","orcid":"https://orcid.org/0000-0003-0624-5263","observed_orcids":["https://orcid.org/0000-0003-0624-5263"]},"institutions":[{"id":"https://openalex.org/I24185976","display_name":"Sichuan University","ror":"https://ror.org/011ashp19","country_code":"CN","type":"education","lineage":["https://openalex.org/I24185976"]},{"id":"https://openalex.org/I4210089761","display_name":"West China Hospital of Sichuan University","ror":"https://ror.org/007mrxy13","country_code":"CN","type":"healthcare","lineage":["https://openalex.org/I4210089761"]},{"id":"https://openalex.org/I4391768003","display_name":"State Key Laboratory of Biotherapy","ror":"https://ror.org/00x43yy22","country_code":"CN","type":"facility","lineage":["https://openalex.org/I24185976","https://openalex.org/I4391768003"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Chengjian Zhao","raw_affiliation_strings":["State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Sichuan, China","institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089761","https://openalex.org/I4391768003"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5034823522","https://openalex.org/A5052964199"],"corresponding_institution_ids":["https://openalex.org/I24185976","https://openalex.org/I4210089761","https://openalex.org/I4391768003"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.4909999966621399,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.4909999966621399,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10158","display_name":"Cancer Immunotherapy and Biomarkers","score":0.38769999146461487,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.06689999997615814,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/tumor-microenvironment","display_name":"tumor microenvironment","score":0.8809999823570251},{"id":"https://openalex.org/keywords/tyramide-signal-amplification","display_name":"tyramide signal amplification","score":0.9179999828338623},{"id":"https://openalex.org/keywords/cell-cell-interactions","display_name":"cell-cell interactions","score":0.746999979019165},{"id":"https://openalex.org/keywords/cell-segmentation","display_name":"cell segmentation","score":0.5630000233650208},{"id":"https://openalex.org/keywords/cell-type-identification","display_name":"cell type annotation","score":0.6169999837875366},{"id":"https://openalex.org/keywords/formalin-fixed-paraffin-embedded-tissue","display_name":"formalin-fixed paraffin-embedded tissue","score":0.8429999947547913},{"id":"https://openalex.org/keywords/computer-vision","display_name":"computer vision","score":0.5410000085830688},{"id":"https://openalex.org/keywords/deep-learning","display_name":"deep learning","score":0.6570000052452087},{"id":"https://openalex.org/keywords/tumor-immunology","display_name":"tumor immunology","score":0.7559999823570251}],"concepts":[{"id":"https://openalex.org/C4224716","wikidata":"https://www.wikidata.org/wiki/Q10859258","display_name":"Immunostaining","level":3,"score":0.557002067565918},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.5494186878204346},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.5058043003082275},{"id":"https://openalex.org/C2776107976","wikidata":"https://www.wikidata.org/wiki/Q1786433","display_name":"Tumor microenvironment","level":3,"score":0.4918794631958008},{"id":"https://openalex.org/C2781188995","wikidata":"https://www.wikidata.org/wiki/Q6934982","display_name":"Multiplex","level":2,"score":0.48389920592308044},{"id":"https://openalex.org/C159620131","wikidata":"https://www.wikidata.org/wiki/Q1938983","display_name":"Spatial analysis","level":2,"score":0.4371830224990845},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.37307560443878174},{"id":"https://openalex.org/C204232928","wikidata":"https://www.wikidata.org/wiki/Q899285","display_name":"Immunohistochemistry","level":2,"score":0.3666521906852722},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3409082889556885},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.226691335439682},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.20149433612823486},{"id":"https://openalex.org/C205649164","wikidata":"https://www.wikidata.org/wiki/Q1071","display_name":"Geography","level":0,"score":0.0987461507320404},{"id":"https://openalex.org/C62649853","wikidata":"https://www.wikidata.org/wiki/Q199687","display_name":"Remote sensing","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1101/2024.08.23.609368","doi":"https://doi.org/10.1101/2024.08.23.609368","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2024.08.23.609368","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.1101/2024.08.23.609368","doi":"https://doi.org/10.1101/2024.08.23.609368","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2024.08.23.609368","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.7246000170707703}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":70,"referenced_works":["https://openalex.org/W1486131515","https://openalex.org/W2019182972","https://openalex.org/W2033278321","https://openalex.org/W2039123767","https://openalex.org/W2043565262","https://openalex.org/W2053129129","https://openalex.org/W2141397097","https://openalex.org/W2168631000","https://openalex.org/W2314057593","https://openalex.org/W2504133172","https://openalex.org/W2554260837","https://openalex.org/W2561429117","https://openalex.org/W2725150247","https://openalex.org/W2743344672","https://openalex.org/W2806530968","https://openalex.org/W2832881433","https://openalex.org/W2905644126","https://openalex.org/W2915848182","https://openalex.org/W2947878607","https://openalex.org/W2949238013","https://openalex.org/W2952481429","https://openalex.org/W2955949220","https://openalex.org/W2956024695","https://openalex.org/W2967934012","https://openalex.org/W2983326574","https://openalex.org/W2998493318","https://openalex.org/W3047081808","https://openalex.org/W3111521801","https://openalex.org/W3121051888","https://openalex.org/W3126210657","https://openalex.org/W3134482219","https://openalex.org/W3144846777","https://openalex.org/W3152880902","https://openalex.org/W3171079601","https://openalex.org/W3172602094","https://openalex.org/W3173767537","https://openalex.org/W3191029526","https://openalex.org/W3206996158","https://openalex.org/W4213197580","https://openalex.org/W4225010526","https://openalex.org/W4282838920","https://openalex.org/W4283032464","https://openalex.org/W4285604883","https://openalex.org/W4295750920","https://openalex.org/W4295923807","https://openalex.org/W4308531634","https://openalex.org/W4318817212","https://openalex.org/W4318831792","https://openalex.org/W4321116368","https://openalex.org/W4322767854","https://openalex.org/W4362704363","https://openalex.org/W4367555025","https://openalex.org/W4385197377","https://openalex.org/W4385610932","https://openalex.org/W4386291641","https://openalex.org/W4386728489","https://openalex.org/W4387709208","https://openalex.org/W4388529939","https://openalex.org/W4388997570","https://openalex.org/W4389245186","https://openalex.org/W4389560413","https://openalex.org/W4389938094","https://openalex.org/W4389986905","https://openalex.org/W4392059486","https://openalex.org/W4393022935","https://openalex.org/W4393381524","https://openalex.org/W4393858351","https://openalex.org/W4393934579","https://openalex.org/W4394998094","https://openalex.org/W4408963522"],"related_works":["https://openalex.org/W7222748481","https://openalex.org/W6911434964","https://openalex.org/W4385687572","https://openalex.org/W7134953071","https://openalex.org/W4287823868","https://openalex.org/W4308378346","https://openalex.org/W4385970631","https://openalex.org/W4282927865","https://openalex.org/W4362535206","https://openalex.org/W7149007452"],"abstract_inverted_index":{"ABSTRACT":[0],"The":[1,17,97],"characteristics":[2],"of":[3,104,147,193,222],"the":[4,72,102,115,135,144,148,189,197,204,217,226],"tumor":[5,12,230],"microenvironment":[6],"(TME)":[7],"are":[8],"closely":[9],"linked":[10],"to":[11,173,213],"progression":[13],"and":[14,28,55,124,177,203,215,220,233],"treatment":[15],"response.":[16],"TME":[18,149,227],"comprises":[19],"various":[20],"cell":[21,179,195],"types,":[22,218],"their":[23,29,61],"spatial":[24,41,145,190],"distribution,":[25],"cell-cell":[26],"interactions,":[27],"organization":[30],"into":[31],"cellular":[32],"niches":[33],"or":[34],"neighborhoods.":[35],"To":[36,157],"capture":[37],"this":[38,68],"complexity,":[39],"several":[40],"profiling":[42],"technologies":[43],"have":[44,59],"been":[45],"developed.":[46],"However,":[47],"challenges":[48],"such":[49],"as":[50],"low":[51],"throughput,":[52],"high":[53],"costs,":[54],"complicated":[56],"data":[57,153],"analysis":[58,165],"limited":[60],"widespread":[62],"use":[63],"in":[64],"immune":[65,223,235],"research.":[66],"In":[67],"study,":[69],"we":[70,160],"introduce":[71],"Cyclic-multiplex":[73],"TSA":[74],"(CmTSA)":[75],"staining":[76,81],"platform,":[77],"a":[78,162],"high-throughput":[79],"superplex":[80,136],"technology":[82],"based":[83,181],"on":[84,182],"tyramide":[85],"signal":[86],"amplification":[87],"(TSA)":[88],"immunostaining":[89],"combined":[90],"with":[91,169],"an":[92],"efficient":[93],"fluorophore":[94],"recycling":[95],"method.":[96],"CmTSA":[98,117],"platform":[99],"allows":[100],"for":[101],"labeling":[103],"30-60":[105],"antigens":[106],"across":[107],"multiple":[108],"parallel":[109],"formalin-fixed":[110],"paraffin-embedded":[111],"(FFPE)":[112],"slides.":[113],"Furthermore,":[114],"automated":[116],"workflow":[118],"requires":[119],"only":[120],"standard":[121],"histological":[122],"equipment":[123],"conventional":[125],"immunohistochemistry":[126],"(IHC)":[127],"primary":[128],"antibodies":[129],"(Abs),":[130],"significantly":[131],"reducing":[132],"costs.":[133],"While":[134],"images":[137],"produced":[138],"contain":[139],"extensive":[140],"multidimensional":[141],"information,":[142],"extracting":[143],"features":[146],"from":[150],"raw":[151],"pixel":[152],"can":[154],"be":[155],"challenging.":[156],"address":[158],"this,":[159],"present":[161],"computer":[163],"vision-based":[164],"pipeline,":[166],"which":[167],"begins":[168],"deep":[170],"learning-based":[171],"algorithms":[172],"segment":[174],"individual":[175],"cells":[176],"identify":[178],"types":[180],"defined":[183],"annotation":[184],"rules.":[185],"It":[186],"then":[187],"evaluates":[188],"distribution":[191],"tendencies":[192],"each":[194],"type,":[196],"interaction":[198],"intensity":[199],"between":[200],"paired":[201],"cells,":[202],"multicellular":[205],"functional":[206],"niches.":[207],"This":[208],"comprehensive":[209],"approach":[210],"enables":[211],"researchers":[212],"visualize":[214],"quantify":[216],"states,":[219],"levels":[221],"activities":[224],"within":[225],"effectively,":[228],"advancing":[229],"immunology":[231],"research":[232],"precision":[234],"medicine.":[236]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":1}],"updated_date":"2026-10-27T15:51:10.671819","created_date":"2024-08-30T00:00:00"}
