{"id":"https://openalex.org/W4401699486","doi":"https://doi.org/10.17504/protocols.io.rm7vzj5d2lx1/v2","title":"AAV Production and Purification v2","display_name":"AAV Production and Purification v2","publication_year":2024,"publication_date":"2024-07-08","ids":{"openalex":"https://openalex.org/W4401699486","doi":"https://doi.org/10.17504/protocols.io.rm7vzj5d2lx1/v2"},"language":"en","primary_location":{"id":"doi:10.17504/protocols.io.rm7vzj5d2lx1/v2","doi":"https://doi.org/10.17504/protocols.io.rm7vzj5d2lx1/v2","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.rm7vzj5d2lx1/v2","pdf_url":"https://www.protocols.io/view/aav-production-and-purification-djkp4kvn.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.protocols.io/view/aav-production-and-purification-djkp4kvn.pdf","any_repository_has_fulltext":null},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5023506109","display_name":"Justin T Savage","orcid":"https://orcid.org/0000-0002-1530-4942","observed_orcids":["https://orcid.org/0000-0002-1530-4942"]},"institutions":[{"id":"https://openalex.org/I170897317","display_name":"Duke University","ror":"https://ror.org/00py81415","country_code":"US","type":"education","lineage":["https://openalex.org/I170897317"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Justin T Savage","raw_affiliation_strings":["Duke University Eroglu_Lab"],"raw_orcid":"https://orcid.org/0000-0002-1530-4942","affiliations":[{"raw_affiliation_string":"Duke University Eroglu_Lab","institution_ids":["https://openalex.org/I170897317"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5023506109"],"corresponding_institution_ids":["https://openalex.org/I170897317"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.007199999876320362,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0019000000320374966,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/adeno-associated-virus","display_name":"adeno-associated virus","score":0.9959999918937683},{"id":"https://openalex.org/keywords/aav-production","display_name":"AAV production","score":0.9760000109672546},{"id":"https://openalex.org/keywords/aav-purification","display_name":"AAV purification","score":0.8989999890327454},{"id":"https://openalex.org/keywords/hek293t-cells","display_name":"HEK293T cells","score":0.9490000009536743},{"id":"https://openalex.org/keywords/viral-vector-production","display_name":"viral vector production","score":0.7070000171661377}],"concepts":[{"id":"https://openalex.org/C2778348673","wikidata":"https://www.wikidata.org/wiki/Q739302","display_name":"Production (economics)","level":2,"score":0.6186096668243408},{"id":"https://openalex.org/C144133560","wikidata":"https://www.wikidata.org/wiki/Q4830453","display_name":"Business","level":0,"score":0.32021525502204895},{"id":"https://openalex.org/C162324750","wikidata":"https://www.wikidata.org/wiki/Q8134","display_name":"Economics","level":0,"score":0.18106487393379211},{"id":"https://openalex.org/C175444787","wikidata":"https://www.wikidata.org/wiki/Q39072","display_name":"Microeconomics","level":1,"score":0.06412136554718018}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.17504/protocols.io.rm7vzj5d2lx1/v2","doi":"https://doi.org/10.17504/protocols.io.rm7vzj5d2lx1/v2","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.rm7vzj5d2lx1/v2","pdf_url":"https://www.protocols.io/view/aav-production-and-purification-djkp4kvn.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.17504/protocols.io.rm7vzj5d2lx1/v2","doi":"https://doi.org/10.17504/protocols.io.rm7vzj5d2lx1/v2","is_oa":true,"landing_page_url":"https://doi.org/10.17504/protocols.io.rm7vzj5d2lx1/v2","pdf_url":"https://www.protocols.io/view/aav-production-and-purification-djkp4kvn.pdf","source":null,"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4401699486.pdf","grobid_xml":"https://content.openalex.org/works/W4401699486.grobid-xml"},"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4401635794","https://openalex.org/W4246642911","https://openalex.org/W4256213978","https://openalex.org/W2913132103","https://openalex.org/W4400038560","https://openalex.org/W2800711204","https://openalex.org/W2890134619","https://openalex.org/W4390915895","https://openalex.org/W7162994802","https://openalex.org/W4402920654"],"abstract_inverted_index":{"This":[0],"protocol":[1,12],"is":[2],"for":[3],"the":[4,14],"production":[5],"and":[6],"purification":[7],"of":[8],"Adeno-Associated":[9],"Virus.":[10],"The":[11],"contains":[13],"necessary":[15],"steps":[16],"to":[17],"produce":[18],"AAVs":[19],"from":[20],"HEK293T":[21],"cell":[22],"cultures.":[23]},"counts_by_year":[],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2024-08-20T00:00:00"}