{"id":"https://openalex.org/W4401688488","doi":"https://doi.org/10.1101/2024.08.17.608385","title":"Manipulating rice canonical G\u03b1 and extra-large G protein subunits for improved agronomic traits","display_name":"Manipulating rice canonical G\u03b1 and extra-large G protein subunits for improved agronomic traits","publication_year":2024,"publication_date":"2024-08-19","ids":{"openalex":"https://openalex.org/W4401688488","doi":"https://doi.org/10.1101/2024.08.17.608385"},"language":"en","primary_location":{"id":"doi:10.1101/2024.08.17.608385","doi":"https://doi.org/10.1101/2024.08.17.608385","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2024.08.17.608385","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"volume":null,"type":"preprint","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.1101/2024.08.17.608385","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5086519459","display_name":"Christian F. Cantos","orcid":"https://orcid.org/0000-0001-6455-0069","observed_orcids":["https://orcid.org/0000-0001-6455-0069"]},"institutions":[{"id":"https://openalex.org/I130769515","display_name":"Pennsylvania State University","ror":"https://ror.org/04p491231","country_code":"US","type":"education","lineage":["https://openalex.org/I130769515"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Christian F. Cantos","raw_affiliation_strings":["Pennsylvania State University"],"raw_orcid":"https://orcid.org/0000-0001-6455-0069","affiliations":[{"raw_affiliation_string":"Pennsylvania State University","institution_ids":["https://openalex.org/I130769515"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5047244531","display_name":"Sarah M. Assmann","orcid":"https://orcid.org/0000-0003-4541-1594","observed_orcids":["https://orcid.org/0000-0003-4541-1594"]},"institutions":[{"id":"https://openalex.org/I130769515","display_name":"Pennsylvania State University","ror":"https://ror.org/04p491231","country_code":"US","type":"education","lineage":["https://openalex.org/I130769515"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Sarah M. Assmann","raw_affiliation_strings":["Pennsylvania Sate University"],"raw_orcid":"https://orcid.org/0000-0003-4541-1594","affiliations":[{"raw_affiliation_string":"Pennsylvania Sate University","institution_ids":["https://openalex.org/I130769515"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5047244531"],"corresponding_institution_ids":["https://openalex.org/I130769515"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.6211000084877014,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10184","display_name":"Plant Molecular Biology Research","score":0.6211000084877014,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12828","display_name":"Plant Genetic and Mutation Studies","score":0.07890000194311142,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12045","display_name":"Rice Cultivation and Yield Improvement","score":0.06939999759197235,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/rice","display_name":"rice","score":0.8769999742507935},{"id":"https://openalex.org/keywords/heterotrimeric-g-proteins","display_name":"heterotrimeric G proteins","score":0.8500000238418579},{"id":"https://openalex.org/keywords/g-protein-alpha-subunit","display_name":"G protein alpha subunit","score":0.7229999899864197},{"id":"https://openalex.org/keywords/rga1","display_name":"RGA1","score":0.8989999890327454},{"id":"https://openalex.org/keywords/plant-architecture","display_name":"plant architecture","score":0.9679999947547913},{"id":"https://openalex.org/keywords/short-stature","display_name":"short stature","score":0.4950000047683716},{"id":"https://openalex.org/keywords/grain-yield","display_name":"grain yield","score":0.7419999837875366},{"id":"https://openalex.org/keywords/spikelet-number","display_name":"spikelet number","score":0.8450000286102295},{"id":"https://openalex.org/keywords/seed-production","display_name":"seed production","score":0.7059999704360962},{"id":"https://openalex.org/keywords/flag-leaf-width","display_name":"flag leaf width","score":0.7770000100135803},{"id":"https://openalex.org/keywords/harvest-index","display_name":"harvest index","score":0.9409999847412109},{"id":"https://openalex.org/keywords/genetic-interactions","display_name":"genetic interactions","score":0.5950000286102295}],"concepts":[{"id":"https://openalex.org/C75337361","wikidata":"https://www.wikidata.org/wiki/Q148600","display_name":"Panicle","level":2,"score":0.8126814365386963},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7837034463882446},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6509833335876465},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6298186182975769},{"id":"https://openalex.org/C106934330","wikidata":"https://www.wikidata.org/wiki/Q1971873","display_name":"Trait","level":2,"score":0.5216758251190186},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5056426525115967},{"id":"https://openalex.org/C81941488","wikidata":"https://www.wikidata.org/wiki/Q853421","display_name":"Quantitative trait locus","level":3,"score":0.4674718379974365},{"id":"https://openalex.org/C139407321","wikidata":"https://www.wikidata.org/wiki/Q408561","display_name":"Heterotrimeric G protein","level":4,"score":0.45499640703201294},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.44274449348449707},{"id":"https://openalex.org/C6557445","wikidata":"https://www.wikidata.org/wiki/Q173113","display_name":"Agronomy","level":1,"score":0.2777233123779297},{"id":"https://openalex.org/C62478195","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signal transduction","level":2,"score":0.13152477145195007},{"id":"https://openalex.org/C80631254","wikidata":"https://www.wikidata.org/wiki/Q407438","display_name":"G protein","level":3,"score":0.08018109202384949},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1101/2024.08.17.608385","doi":"https://doi.org/10.1101/2024.08.17.608385","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2024.08.17.608385","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"}],"best_oa_location":{"id":"doi:10.1101/2024.08.17.608385","doi":"https://doi.org/10.1101/2024.08.17.608385","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2024.08.17.608385","pdf_url":null,"source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/2","score":0.9208999872207642,"display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W1845286340","https://openalex.org/W1975436666","https://openalex.org/W1979971043","https://openalex.org/W1983308672","https://openalex.org/W1984425875","https://openalex.org/W2042386169","https://openalex.org/W2045857683","https://openalex.org/W2100853994","https://openalex.org/W2106737335","https://openalex.org/W2112794695","https://openalex.org/W2140339883","https://openalex.org/W2151572706","https://openalex.org/W2169981212","https://openalex.org/W2169998509","https://openalex.org/W2397673202","https://openalex.org/W2460640366","https://openalex.org/W2565884518","https://openalex.org/W2768019707","https://openalex.org/W2771512395","https://openalex.org/W2801362130","https://openalex.org/W2804713482","https://openalex.org/W2907032736","https://openalex.org/W2965877173","https://openalex.org/W3012589727","https://openalex.org/W3172734374","https://openalex.org/W3199486103","https://openalex.org/W3210843316","https://openalex.org/W4206240398","https://openalex.org/W4210707335","https://openalex.org/W4226236621","https://openalex.org/W4229453373","https://openalex.org/W4280494250","https://openalex.org/W4306155346","https://openalex.org/W4306407003","https://openalex.org/W4375847037","https://openalex.org/W4390747641","https://openalex.org/W4392747552","https://openalex.org/W4409205362"],"related_works":["https://openalex.org/W4206240398","https://openalex.org/W3124739274","https://openalex.org/W4375847037","https://openalex.org/W3012589727","https://openalex.org/W2889523948","https://openalex.org/W4400580672","https://openalex.org/W4235609025","https://openalex.org/W4392372441","https://openalex.org/W2804517857","https://openalex.org/W2923024264"],"abstract_inverted_index":{"Abstract":[0],"Rice":[1],"productivity":[2],"is":[3,212],"fundamentally":[4],"linked":[5],"to":[6,141,244,260],"its":[7],"architecture,":[8,241],"governed":[9],"by":[10,177],"signaling":[11,236],"networks":[12,237],"including":[13],"those":[14],"based":[15],"on":[16],"heterotrimeric":[17],"G":[18],"proteins.":[19],"In":[20,91],"this":[21],"study,":[22],"we":[23],"investigated":[24],"the":[25,33,41,84,128,160,172,183,227,235],"individual":[26],"gene":[27,36,62],"impacts":[28],"and":[29,40,67,125,132,166,195,232],"genetic":[30],"interactions":[31],"of":[32,74,111,130,152,201,230,251],"canonical":[34],"G\u03b1":[35,44,112],"(":[37,46],"RGA1":[38,75,97,179,202,210,231],"),":[39],"non-canonical":[42],"extra-large":[43],"genes":[45],"OsXLG1,":[47,253],"OsXLG3a,":[48,254],"OsXLG3b,":[49],"OsXLG4":[50,104,126,256],")":[51],"in":[52,64,83,96,115,123,145,182],"controlling":[53],"plant":[54],"architecture.":[55],"We":[56,77],"generated":[57],"OsXLG":[58,157,199],"mutants":[59,185],"using":[60],"CRISPR/Cpf1":[61],"editing":[63],"Nipponbare":[65,71],"(WT)":[66],"d1":[68,161],",":[69,98,100,102,105,147,149,154],"a":[70,116,119],"null":[72],"mutant":[73],".":[76],"then":[78],"phenotyped":[79],"25":[80],"different":[81,88],"genotypes":[82],"greenhouse":[85],"for":[86],"19":[87],"agronomic":[89],"traits.":[90,223],"wild":[92],"type":[93],"(WT),":[94],"mutations":[95],"OsXLG3a":[99,146],"OsXLG3b":[101,148],"or":[103,155,255],"as":[106,108,204,258],"well":[107],"any":[109,150,156],"combination":[110,151],"genes,":[113],"resulted":[114],"shorter":[117],"stature,":[118],"desirable":[120],"trait.":[121],"Mutations":[122,144],"OsXLG1":[124],"increased":[127,209],"number":[129],"spikelets":[131],"grains":[133],"per":[134],"panicle,":[135],"showcasing":[136],"advantageous":[137],"traits":[138,218],"that":[139,238],"led":[140],"higher":[142],"yield.":[143,167,262],"OsXLGs":[153,233],"combined":[158],"with":[159,189,214],"mutation,":[162],"reduced":[163],"seed":[164],"production":[165],"Flag":[168],"leaf":[169],"width":[170],"was":[171,186],"only":[173],"trait":[174],"influenced":[175],"solely":[176],"RGA1.":[178],"transcript":[180],"abundance":[181],"osxlg":[184],"positively":[187],"correlated":[188,213],"height,":[190],"culm":[191],"length,":[192],"panicle":[193],"exsertion,":[194],"harvest":[196],"index,":[197],"implicating":[198],"regulation":[200],"expression":[203,211,257],"an":[205],"underlying":[206],"mechanism.":[207],"Overall,":[208],"more":[215],"favorable":[216,221],"reproductive":[217],"but":[219],"less":[220],"vegetative":[222,243],"Our":[224,247],"study":[225],"reveals":[226],"complex":[228],"interaction":[229],"within":[234],"shape":[239],"rice":[240],"from":[242],"post-harvest":[245],"stages.":[246],"results":[248],"suggest":[249],"modulation":[250],"RGA1,":[252],"strategies":[259],"enhance":[261]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2024-08-19T00:00:00"}