{"id":"https://openalex.org/W4401428534","doi":"https://doi.org/10.1364/ol.530358","title":"Fundamental precision limits of fluorescence microscopy: a perspective on MINFLUX","display_name":"Fundamental precision limits of fluorescence microscopy: a perspective on MINFLUX","publication_year":2024,"publication_date":"2024-08-08","ids":{"openalex":"https://openalex.org/W4401428534","doi":"https://doi.org/10.1364/ol.530358","pmid":"https://pubmed.ncbi.nlm.nih.gov/39208007"},"language":"en","primary_location":{"id":"doi:10.1364/ol.530358","doi":"https://doi.org/10.1364/ol.530358","is_oa":false,"landing_page_url":"https://doi.org/10.1364/ol.530358","pdf_url":null,"source":{"id":"https://openalex.org/S134390580","display_name":"Optics Letters","issn_l":"0146-9592","issn":["0146-9592","1071-2763","1539-4794"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315679","host_organization_name":"Optica Publishing Group","host_organization_lineage":["https://openalex.org/P4310315679"],"host_organization_lineage_names":["Optica Publishing Group"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Optics Letters","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5011890598","display_name":"Matteo Rosati","orcid":"https://orcid.org/0000-0002-8972-2936","observed_orcids":["https://orcid.org/0000-0002-8972-2936"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Matteo Rosati","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-8972-2936","affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070829468","display_name":"Miranda Parisi","orcid":"https://orcid.org/0000-0002-4682-4414","observed_orcids":["https://orcid.org/0000-0002-4682-4414"]},"institutions":[{"id":"https://openalex.org/I119003972","display_name":"Roma Tre University","ror":"https://ror.org/05vf0dg29","country_code":"IT","type":"education","lineage":["https://openalex.org/I119003972"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Miranda Parisi","raw_affiliation_strings":["Universit\u00e0 degli Studi Roma Tre"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Universit\u00e0 degli Studi Roma Tre","institution_ids":["https://openalex.org/I119003972"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066725070","display_name":"Ilaria Gianani","orcid":"https://orcid.org/0000-0002-0674-767X","observed_orcids":["https://orcid.org/0000-0002-0674-767X"]},"institutions":[{"id":"https://openalex.org/I119003972","display_name":"Roma Tre University","ror":"https://ror.org/05vf0dg29","country_code":"IT","type":"education","lineage":["https://openalex.org/I119003972"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Ilaria Gianani","raw_affiliation_strings":["Universit\u00e0 degli Studi Roma Tre"],"raw_orcid":"https://orcid.org/0000-0002-0674-767X","affiliations":[{"raw_affiliation_string":"Universit\u00e0 degli Studi Roma Tre","institution_ids":["https://openalex.org/I119003972"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073504916","display_name":"Marco Barbieri","orcid":"https://orcid.org/0000-0003-2057-9104","observed_orcids":["https://orcid.org/0000-0003-2057-9104"]},"institutions":[{"id":"https://openalex.org/I119003972","display_name":"Roma Tre University","ror":"https://ror.org/05vf0dg29","country_code":"IT","type":"education","lineage":["https://openalex.org/I119003972"]},{"id":"https://openalex.org/I4210120496","display_name":"National Institute of Optics","ror":"https://ror.org/02dp3a879","country_code":"IT","type":"facility","lineage":["https://openalex.org/I4210120496","https://openalex.org/I4210155236"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"Marco Barbieri","raw_affiliation_strings":["Istituto Nazionale di Ottica \u2013 CNR","Universit\u00e0 degli Studi Roma Tre"],"raw_orcid":"https://orcid.org/0000-0003-2057-9104","affiliations":[{"raw_affiliation_string":"Istituto Nazionale di Ottica \u2013 CNR","institution_ids":["https://openalex.org/I4210120496"]},{"raw_affiliation_string":"Universit\u00e0 degli Studi Roma Tre","institution_ids":["https://openalex.org/I119003972"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5037841440","display_name":"Gabriella Cincotti","orcid":"https://orcid.org/0000-0002-9139-1697","observed_orcids":["https://orcid.org/0000-0002-9139-1697"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Gabriella Cincotti","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-9139-1697","affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":0.3866,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.52291702,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":94,"max":96},"biblio":{"volume":"49","issue":"17","first_page":"4938","last_page":"4938"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11484","display_name":"Adaptive optics and wavefront sensing","score":0.0,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10923","display_name":"Force Microscopy Techniques and Applications","score":0.0,"subfield":{"id":"https://openalex.org/subfields/3107","display_name":"Atomic and Molecular Physics, and Optics"},"field":{"id":"https://openalex.org/fields/31","display_name":"Physics and Astronomy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/localization-precision","display_name":"localization precision","score":0.8539999723434448},{"id":"https://openalex.org/keywords/multiparameter-estimation","display_name":"multiparameter estimation","score":0.949999988079071},{"id":"https://openalex.org/keywords/photobleaching","display_name":"photobleaching","score":0.7400000095367432},{"id":"https://openalex.org/keywords/background-noise","display_name":"background noise","score":0.7580000162124634},{"id":"https://openalex.org/keywords/beam-separation","display_name":"beam separation","score":0.7179999947547913},{"id":"https://openalex.org/keywords/stimulated-emission-depletion","display_name":"stimulated emission depletion","score":0.7670000195503235}],"concepts":[{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.7885488271713257},{"id":"https://openalex.org/C147080431","wikidata":"https://www.wikidata.org/wiki/Q1074953","display_name":"Microscopy","level":2,"score":0.6779266595840454},{"id":"https://openalex.org/C116919411","wikidata":"https://www.wikidata.org/wiki/Q1522462","display_name":"Photoactivated localization microscopy","level":5,"score":0.6250193119049072},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.5931552648544312},{"id":"https://openalex.org/C113976765","wikidata":"https://www.wikidata.org/wiki/Q1823502","display_name":"Light sheet fluorescence microscopy","level":4,"score":0.5404973030090332},{"id":"https://openalex.org/C12713177","wikidata":"https://www.wikidata.org/wiki/Q1900281","display_name":"Perspective (graphical)","level":2,"score":0.5043367147445679},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.46837812662124634},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.43339216709136963},{"id":"https://openalex.org/C166936260","wikidata":"https://www.wikidata.org/wiki/Q7642959","display_name":"Super-resolution microscopy","level":4,"score":0.3771839439868927},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.28539296984672546},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.20382806658744812},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.10357478260993958}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.1364/ol.530358","doi":"https://doi.org/10.1364/ol.530358","is_oa":false,"landing_page_url":"https://doi.org/10.1364/ol.530358","pdf_url":null,"source":{"id":"https://openalex.org/S134390580","display_name":"Optics Letters","issn_l":"0146-9592","issn":["0146-9592","1071-2763","1539-4794"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315679","host_organization_name":"Optica Publishing Group","host_organization_lineage":["https://openalex.org/P4310315679"],"host_organization_lineage_names":["Optica Publishing Group"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Optics Letters","raw_type":"journal-article"},{"id":"pmid:39208007","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/39208007","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Optics letters","raw_type":"Journal Article"},{"id":"pmh:oai:iris.uniroma3.it:11590/481527","doi":null,"is_oa":false,"landing_page_url":"https://hdl.handle.net/11590/481527","pdf_url":null,"source":{"id":"https://openalex.org/S4377196120","display_name":"Iris (Roma Tre University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I119003972","host_organization_name":"Roma Tre University","host_organization_lineage":["https://openalex.org/I119003972"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G6329346359","display_name":null,"funder_award_id":"STORMYTUNE 899587","funder_id":"https://openalex.org/F4320338336","funder_display_name":"H2020 Future and Emerging Technologies"},{"id":"https://openalex.org/G8352141449","display_name":null,"funder_award_id":"816000-2022-SQUID - CUP F83C22002390007","funder_id":"https://openalex.org/F4320331528","funder_display_name":"Ministero dell'Universit\u00e0 e della Ricerca"}],"funders":[{"id":"https://openalex.org/F4320331528","display_name":"Ministero dell'Universit\u00e0 e della Ricerca","ror":"https://ror.org/0341vw408"},{"id":"https://openalex.org/F4320338336","display_name":"H2020 Future and Emerging Technologies","ror":null}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W1650079475","https://openalex.org/W2062552264","https://openalex.org/W2075030758","https://openalex.org/W2078380944","https://openalex.org/W2115737236","https://openalex.org/W2152708992","https://openalex.org/W2169791141","https://openalex.org/W2171820152","https://openalex.org/W2174811772","https://openalex.org/W2316993869","https://openalex.org/W2465598250","https://openalex.org/W2554260837","https://openalex.org/W2808888199","https://openalex.org/W2901691361","https://openalex.org/W2903629831","https://openalex.org/W2998779366","https://openalex.org/W3138916068","https://openalex.org/W3167557279","https://openalex.org/W3196337473","https://openalex.org/W4213267971","https://openalex.org/W4245030170","https://openalex.org/W4281480406","https://openalex.org/W4281489135","https://openalex.org/W4308458879","https://openalex.org/W4310098528","https://openalex.org/W4311679901","https://openalex.org/W4312754905","https://openalex.org/W4378070234","https://openalex.org/W4388078038"],"related_works":["https://openalex.org/W4382618943","https://openalex.org/W4403578067","https://openalex.org/W2809085822","https://openalex.org/W2887557100","https://openalex.org/W7122810251","https://openalex.org/W3133879440","https://openalex.org/W7222635990","https://openalex.org/W4400503023","https://openalex.org/W4229062061","https://openalex.org/W3187607029"],"abstract_inverted_index":{"Over":[0],"the":[1,12,38,48,67,73,82,88,97,107,112,133],"past":[2],"years,":[3],"fluorescence":[4],"microscopy":[5],"(FM)":[6],"has":[7],"steadily":[8],"progressed":[9],"in":[10,18,102],"increasing":[11,87],"localization":[13,55,74,108],"precision":[14,75,109],"of":[15,40,51,90,104,132],"fluorescent":[16],"emitters":[17],"biological":[19],"samples":[20],"and":[21,92,100],"led":[22],"to":[23,37,120,140],"new":[24],"claims,":[25],"whose":[26],"rigorous":[27],"validation":[28],"remains":[29],"an":[30],"outstanding":[31],"problem.":[32],"We":[33,57],"present":[34],"a":[35,52,130],"novel,":[36],"best":[39],"our":[41,59,118],"knowledge,":[42],"multi-parameter":[43],"estimation":[44],"framework":[45],"that":[46,71,129],"captures":[47],"full":[49],"complexity":[50],"single-emitter":[53],"FM":[54],"experiment.":[56],"showcase":[58],"method":[60,119],"with":[61,111],"Minimum":[62,121],"Flux":[63,122],"(MINFLUX)":[64],"microscopy,":[65,127],"among":[66],"highest-resolution":[68],"approaches,":[69],"demonstrating":[70],"(i)":[72],"can":[76,136],"be":[77],"increased":[78],"only":[79],"by":[80],"turning":[81],"illumination":[83],"intensity":[84],"up,":[85],"thus":[86],"risk":[89],"photo-bleaching,":[91],"it":[93],"is":[94],"independent":[95],"from":[96],"beams'":[98,113],"separation,":[99],"(ii)":[101],"presence":[103],"background":[105],"noise,":[106],"decreases":[110],"separation.":[114],"Finally,":[115],"we":[116],"apply":[117],"Stimulated":[123],"Emission":[124],"Depletion":[125],"(MINSTED)":[126],"showing":[128],"reduction":[131],"beam":[134],"width":[135],"provide":[137],"similar":[138],"performance":[139],"MINFLUX.":[141]},"counts_by_year":[{"year":2025,"cited_by_count":2}],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2024-08-09T00:00:00"}