{"id":"https://openalex.org/W4401190302","doi":"https://doi.org/10.1016/j.xpro.2024.103221","title":"Protocol for establishing inducible CRISPR interference system for multiple-gene silencing in human pluripotent stem cells","display_name":"Protocol for establishing inducible CRISPR interference system for multiple-gene silencing in human pluripotent stem cells","publication_year":2024,"publication_date":"2024-07-31","ids":{"openalex":"https://openalex.org/W4401190302","doi":"https://doi.org/10.1016/j.xpro.2024.103221","pmid":"https://pubmed.ncbi.nlm.nih.gov/39083383","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/11342178"},"language":"en","primary_location":{"id":"doi:10.1016/j.xpro.2024.103221","doi":"https://doi.org/10.1016/j.xpro.2024.103221","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xpro.2024.103221","pdf_url":null,"source":{"id":"https://openalex.org/S4210212477","display_name":"STAR Protocols","issn_l":"2666-1667","issn":["2666-1667"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protocols","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1016/j.xpro.2024.103221","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Satoshi Matsui","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1285204247","display_name":"Cincinnati Children's Hospital Medical Center","ror":"https://ror.org/01hcyya48","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1285204247"]},{"id":"https://openalex.org/I63135867","display_name":"University of Cincinnati","ror":"https://ror.org/01e3m7079","country_code":"US","type":"education","lineage":["https://openalex.org/I63135867"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Satoshi Matsui","raw_affiliation_strings":["Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA","institution_ids":["https://openalex.org/I1285204247","https://openalex.org/I63135867"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004946112","display_name":"Joseph R Shiley","orcid":"https://orcid.org/0000-0001-8453-1738","observed_orcids":["https://orcid.org/0000-0001-8453-1738"]},"institutions":[{"id":"https://openalex.org/I1285204247","display_name":"Cincinnati Children's Hospital Medical Center","ror":"https://ror.org/01hcyya48","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1285204247"]},{"id":"https://openalex.org/I63135867","display_name":"University of Cincinnati","ror":"https://ror.org/01e3m7079","country_code":"US","type":"education","lineage":["https://openalex.org/I63135867"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joseph R. Shiley","raw_affiliation_strings":["Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA","institution_ids":["https://openalex.org/I1285204247","https://openalex.org/I63135867"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5064654485","display_name":"Marissa Granitto","orcid":"https://orcid.org/0000-0002-1758-9881","observed_orcids":["https://orcid.org/0000-0002-1758-9881"]},"institutions":[{"id":"https://openalex.org/I1285204247","display_name":"Cincinnati Children's Hospital Medical Center","ror":"https://ror.org/01hcyya48","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1285204247"]},{"id":"https://openalex.org/I63135867","display_name":"University of Cincinnati","ror":"https://ror.org/01e3m7079","country_code":"US","type":"education","lineage":["https://openalex.org/I63135867"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Marissa Granitto","raw_affiliation_strings":["Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA","institution_ids":["https://openalex.org/I1285204247","https://openalex.org/I63135867"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113092069","display_name":"Katie Ludwig","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1285204247","display_name":"Cincinnati Children's Hospital Medical Center","ror":"https://ror.org/01hcyya48","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1285204247"]},{"id":"https://openalex.org/I63135867","display_name":"University of Cincinnati","ror":"https://ror.org/01e3m7079","country_code":"US","type":"education","lineage":["https://openalex.org/I63135867"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Katie Ludwig","raw_affiliation_strings":["Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA","institution_ids":["https://openalex.org/I1285204247","https://openalex.org/I63135867"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037051019","display_name":"Morgan Buckley","orcid":"https://orcid.org/0009-0008-2015-7730","observed_orcids":["https://orcid.org/0009-0008-2015-7730"]},"institutions":[{"id":"https://openalex.org/I1285204247","display_name":"Cincinnati Children's Hospital Medical Center","ror":"https://ror.org/01hcyya48","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1285204247"]},{"id":"https://openalex.org/I63135867","display_name":"University of Cincinnati","ror":"https://ror.org/01e3m7079","country_code":"US","type":"education","lineage":["https://openalex.org/I63135867"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Morgan Buckley","raw_affiliation_strings":["Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA","institution_ids":["https://openalex.org/I1285204247","https://openalex.org/I63135867"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5093698800","display_name":"Sandra Koigi","orcid":"https://orcid.org/0009-0009-0786-0840","observed_orcids":["https://orcid.org/0009-0009-0786-0840"]},"institutions":[{"id":"https://openalex.org/I1285204247","display_name":"Cincinnati Children's Hospital Medical Center","ror":"https://ror.org/01hcyya48","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1285204247"]},{"id":"https://openalex.org/I63135867","display_name":"University of Cincinnati","ror":"https://ror.org/01e3m7079","country_code":"US","type":"education","lineage":["https://openalex.org/I63135867"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sandra Koigi","raw_affiliation_strings":["Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA","institution_ids":["https://openalex.org/I1285204247","https://openalex.org/I63135867"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079763732","display_name":"Gerardo Gabriel Mirizio","orcid":"https://orcid.org/0000-0003-4774-3445","observed_orcids":["https://orcid.org/0000-0003-4774-3445"]},"institutions":[{"id":"https://openalex.org/I1285204247","display_name":"Cincinnati Children's Hospital Medical Center","ror":"https://ror.org/01hcyya48","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1285204247"]},{"id":"https://openalex.org/I63135867","display_name":"University of Cincinnati","ror":"https://ror.org/01e3m7079","country_code":"US","type":"education","lineage":["https://openalex.org/I63135867"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gerardo Mirizio","raw_affiliation_strings":["Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA","institution_ids":["https://openalex.org/I1285204247","https://openalex.org/I63135867"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009851124","display_name":"Yueh\u2010Chiang Hu","orcid":"https://orcid.org/0000-0002-7992-0525","observed_orcids":["https://orcid.org/0000-0002-7992-0525"]},"institutions":[{"id":"https://openalex.org/I1285204247","display_name":"Cincinnati Children's Hospital Medical Center","ror":"https://ror.org/01hcyya48","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1285204247"]},{"id":"https://openalex.org/I63135867","display_name":"University of Cincinnati","ror":"https://ror.org/01e3m7079","country_code":"US","type":"education","lineage":["https://openalex.org/I63135867"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yueh-Chiang Hu","raw_affiliation_strings":["Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA","institution_ids":["https://openalex.org/I1285204247","https://openalex.org/I63135867"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068495574","display_name":"Christopher N. Mayhew","orcid":"https://orcid.org/0000-0002-7408-1726","observed_orcids":["https://orcid.org/0000-0002-7408-1726"]},"institutions":[{"id":"https://openalex.org/I1285204247","display_name":"Cincinnati Children's Hospital Medical Center","ror":"https://ror.org/01hcyya48","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1285204247"]},{"id":"https://openalex.org/I63135867","display_name":"University of Cincinnati","ror":"https://ror.org/01e3m7079","country_code":"US","type":"education","lineage":["https://openalex.org/I63135867"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Christopher N. Mayhew","raw_affiliation_strings":["Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA. Electronic address: christopher.mayhew@cchmc.org"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA. Electronic address: christopher.mayhew@cchmc.org","institution_ids":["https://openalex.org/I1285204247","https://openalex.org/I63135867"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5045877029","display_name":"Makiko Iwafuchi","orcid":"https://orcid.org/0000-0003-1291-6246","observed_orcids":["https://orcid.org/0000-0003-1291-6246"]},"institutions":[{"id":"https://openalex.org/I1285204247","display_name":"Cincinnati Children's Hospital Medical Center","ror":"https://ror.org/01hcyya48","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1285204247"]},{"id":"https://openalex.org/I63135867","display_name":"University of Cincinnati","ror":"https://ror.org/01e3m7079","country_code":"US","type":"education","lineage":["https://openalex.org/I63135867"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Makiko Iwafuchi","raw_affiliation_strings":["Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA. Electronic address: makiko.iwafuchi@cchmc.org"],"raw_orcid":"https://orcid.org/0000-0003-1291-6246","affiliations":[{"raw_affiliation_string":"Division of Developmental Biology, Center for Stem Cell & Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Department of Pediatrics, College of Medicine, University of Cincinnati, Cincinnati, OH 45229, USA. Electronic address: makiko.iwafuchi@cchmc.org","institution_ids":["https://openalex.org/I1285204247","https://openalex.org/I63135867"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5045877029","https://openalex.org/A5068495574"],"corresponding_institution_ids":["https://openalex.org/I1285204247","https://openalex.org/I63135867"],"apc_list":{"value":1500,"currency":"USD","value_usd":1500},"apc_paid":{"value":1500,"currency":"USD","value_usd":1500},"fwci":0.2302,"has_fulltext":true,"cited_by_count":2,"citation_normalized_percentile":{"value":0.41297487,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"5","issue":"3","first_page":"103221","last_page":"103221"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9883000254631042,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9883000254631042,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.011099999770522118,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crispr-interference","display_name":"CRISPR interference","score":0.9959999918937683},{"id":"https://openalex.org/keywords/human-induced-pluripotent-stem-cells","display_name":"human induced pluripotent stem cells","score":0.9309999942779541},{"id":"https://openalex.org/keywords/single-guide-rna-design","display_name":"single guide RNA design","score":0.9269999861717224},{"id":"https://openalex.org/keywords/loss-of-function-screen","display_name":"loss-of-function screen","score":0.7369999885559082}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.7687696218490601},{"id":"https://openalex.org/C107459253","wikidata":"https://www.wikidata.org/wiki/Q674925","display_name":"Induced pluripotent stem cell","level":4,"score":0.7657893896102905},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6448480486869812},{"id":"https://openalex.org/C166703698","wikidata":"https://www.wikidata.org/wiki/Q201993","display_name":"RNA interference","level":4,"score":0.5663228034973145},{"id":"https://openalex.org/C119056186","wikidata":"https://www.wikidata.org/wiki/Q1431332","display_name":"Gene silencing","level":3,"score":0.5457784533500671},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5136811137199402},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.48460492491722107},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.48153311014175415},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.44556888937950134},{"id":"https://openalex.org/C77957584","wikidata":"https://www.wikidata.org/wiki/Q1133550","display_name":"Gene knockout","level":3,"score":0.42720723152160645},{"id":"https://openalex.org/C97702854","wikidata":"https://www.wikidata.org/wiki/Q4039747","display_name":"Guide RNA","level":5,"score":0.4268030822277069},{"id":"https://openalex.org/C14036430","wikidata":"https://www.wikidata.org/wiki/Q3736076","display_name":"Function (biology)","level":2,"score":0.41697007417678833},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.41527673602104187},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.413105309009552},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.257972776889801},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.13628438115119934},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.10416185855865479},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000094704","descriptor_name":"RNA, Guide, CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004318","descriptor_name":"Doxycycline","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016086","descriptor_name":"Lentivirus","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D020868","descriptor_name":"Gene Silencing","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D039904","descriptor_name":"Pluripotent Stem Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D039904","descriptor_name":"Pluripotent Stem Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D057026","descriptor_name":"Induced Pluripotent Stem Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D057026","descriptor_name":"Induced Pluripotent Stem Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D064112","descriptor_name":"Clustered Regularly Interspaced Short Palindromic Repeats","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1016/j.xpro.2024.103221","doi":"https://doi.org/10.1016/j.xpro.2024.103221","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xpro.2024.103221","pdf_url":null,"source":{"id":"https://openalex.org/S4210212477","display_name":"STAR Protocols","issn_l":"2666-1667","issn":["2666-1667"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protocols","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:11342178","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11342178","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11342178/pdf/main.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protoc","raw_type":"Text"},{"id":"pmid:39083383","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/39083383","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR protocols","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/j.xpro.2024.103221","doi":"https://doi.org/10.1016/j.xpro.2024.103221","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.xpro.2024.103221","pdf_url":null,"source":{"id":"https://openalex.org/S4210212477","display_name":"STAR Protocols","issn_l":"2666-1667","issn":["2666-1667"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"STAR Protocols","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.504800021648407,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1702391210","display_name":"\u4eba\u5de5\u4ea4\u914d\u306b\u3088\u308b\u30ab\u30d6\u30c8\u30ac\u30cb\u73fe\u5b584\u7a2e\u306e\u7cfb\u7d71\u306b\u95a2\u3059\u308b\u7814\u7a76","funder_award_id":"354277","funder_id":"https://openalex.org/F4320334764","funder_display_name":"Japan Society for the Promotion of Science"},{"id":"https://openalex.org/G9000881299","display_name":"Digestive Health Center (DHC): Bench to Bedside Research in Pediatric Digestive Disease","funder_award_id":"P30DK078392","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G9000906653","display_name":"Dynamic regulation of lineage-specific Polycomb repressive landscapes by pioneer and PRDM transcription factors","funder_award_id":"R01GM143161","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320309575","display_name":"Cincinnati Children's Hospital Medical Center","ror":"https://ror.org/01hcyya48"},{"id":"https://openalex.org/F4320310760","display_name":"Uehara Memorial Foundation","ror":"https://ror.org/00gc20a07"},{"id":"https://openalex.org/F4320311959","display_name":"Cincinnati Children\u2019s Research Foundation","ror":"https://ror.org/01hcyya48"},{"id":"https://openalex.org/F4320320212","display_name":"Japan Society for the Promotion of Science London","ror":"https://ror.org/02m7axw05"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4401190302.pdf","grobid_xml":"https://content.openalex.org/works/W4401190302.grobid-xml"},"referenced_works_count":22,"referenced_works":["https://openalex.org/W1965813899","https://openalex.org/W1977709885","https://openalex.org/W2068427957","https://openalex.org/W2101749257","https://openalex.org/W2126259097","https://openalex.org/W2188223075","https://openalex.org/W2207370113","https://openalex.org/W2295326929","https://openalex.org/W2523099503","https://openalex.org/W2745367686","https://openalex.org/W2803479625","https://openalex.org/W3000424462","https://openalex.org/W3002468721","https://openalex.org/W3091008282","https://openalex.org/W3203843972","https://openalex.org/W3211497439","https://openalex.org/W4296116764","https://openalex.org/W4386636748","https://openalex.org/W4388516449","https://openalex.org/W4389436538","https://openalex.org/W4390705145","https://openalex.org/W4391026674"],"related_works":["https://openalex.org/W4401494130","https://openalex.org/W6995667547","https://openalex.org/W7075582604","https://openalex.org/W4412035106","https://openalex.org/W2734972576","https://openalex.org/W4406165189","https://openalex.org/W2295326929","https://openalex.org/W7162671705","https://openalex.org/W3017493802","https://openalex.org/W3181557762"],"abstract_inverted_index":{"Inducible":[0],"loss-of-function":[1],"strategies":[2],"are":[3],"crucial":[4],"for":[5,24,48,77],"understanding":[6],"gene":[7],"function.":[8],"However,":[9],"creating":[10],"inducible,":[11],"multiple-gene":[12],"knockout":[13],"models":[14],"is":[15],"challenging":[16],"and":[17,54,63,88],"time-consuming.":[18],"Here,":[19],"we":[20],"present":[21],"a":[22,26,60],"protocol":[23],"establishing":[25,49,64],"doxycycline-inducible":[27],"CRISPR":[28],"interference":[29],"(CRISPRi)":[30],"system":[31],"to":[32,95],"concurrently":[33],"silence":[34],"multiple":[35],"genes":[36],"in":[37],"human":[38],"induced":[39],"pluripotent":[40],"stem":[41],"cells":[42],"(hPSCs).":[43],"We":[44,72],"describe":[45],"the":[46,75,86],"steps":[47],"host":[50],"CRISPRi":[51,66,80],"hPSCs,":[52],"designing":[53],"cloning":[55],"single-guide":[56],"RNAs":[57],"(sgRNAs)":[58],"into":[59],"lentivirus":[61],"plasmid,":[62],"monoclonal":[65],"hPSC":[67],"lines":[68],"transduced":[69],"with":[70],"sgRNAs.":[71],"also":[73],"detail":[74],"procedures":[76],"selecting":[78],"effective":[79],"clones.":[81],"For":[82],"complete":[83],"details":[84],"on":[85],"use":[87],"execution":[89],"of":[90],"this":[91],"protocol,":[92],"please":[93],"refer":[94],"Matsui":[96],"et":[97],"al.(1)":[98]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1}],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2024-08-01T00:00:00"}