{"id":"https://openalex.org/W4401048937","doi":"https://doi.org/10.1134/s000368382460444x","title":"Bacterial Expression of Crm197: Investigation and Optimization of Gene Expression Factors for Effective Production in E. coli","display_name":"Bacterial Expression of Crm197: Investigation and Optimization of Gene Expression Factors for Effective Production in E. coli","publication_year":2024,"publication_date":"2024-07-27","ids":{"openalex":"https://openalex.org/W4401048937","doi":"https://doi.org/10.1134/s000368382460444x"},"language":"en","primary_location":{"id":"doi:10.1134/s000368382460444x","doi":"https://doi.org/10.1134/s000368382460444x","is_oa":false,"landing_page_url":"https://doi.org/10.1134/s000368382460444x","pdf_url":null,"source":{"id":"https://openalex.org/S37200030","display_name":"Applied Biochemistry and Microbiology","issn_l":"0003-6838","issn":["0003-6838","1608-3024","3034-574X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","norway-1","poland-20","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320267","host_organization_name":"Pleiades Publishing","host_organization_lineage":["https://openalex.org/P4310320267","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Pleiades Publishing","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Biochemistry and Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5007107410","display_name":"S. O. Rogozhkin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210103655","display_name":"Vyatka State University","ror":"https://ror.org/01anxqg21","country_code":"RU","type":"education","lineage":["https://openalex.org/I4210103655"]}],"countries":["RU"],"is_corresponding":true,"raw_author_name":"S. O. Rogozhkin","raw_affiliation_strings":["Vyatka State University, 610000, Kirov, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Vyatka State University, 610000, Kirov, Russia","institution_ids":["https://openalex.org/I4210103655"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5005373429","display_name":"Andrey S. Gerasimov","orcid":"https://orcid.org/0000-0002-3897-0622","observed_orcids":["https://orcid.org/0000-0002-3897-0622"]},"institutions":[{"id":"https://openalex.org/I4210103655","display_name":"Vyatka State University","ror":"https://ror.org/01anxqg21","country_code":"RU","type":"education","lineage":["https://openalex.org/I4210103655"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"A. S. Gerasimov","raw_affiliation_strings":["Vyatka State University, 610000, Kirov, Russia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Vyatka State University, 610000, Kirov, Russia","institution_ids":["https://openalex.org/I4210103655"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5007107410"],"corresponding_institution_ids":["https://openalex.org/I4210103655"],"apc_list":null,"apc_paid":null,"fwci":0.1183,"has_fulltext":false,"cited_by_count":1,"citation_normalized_percentile":{"value":0.34105629,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":92,"max":95},"biblio":{"volume":"60","issue":"4","first_page":"730","last_page":"739"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9761000275611877,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.9761000275611877,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.005900000222027302,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.0031999999191612005,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/crm197","display_name":"CRM197","score":0.996999979019165},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.8119999766349792},{"id":"https://openalex.org/keywords/sumo-fusion-protein","display_name":"SUMO fusion protein","score":0.7900000214576721},{"id":"https://openalex.org/keywords/inclusion-bodies","display_name":"inclusion bodies","score":0.9490000009536743},{"id":"https://openalex.org/keywords/protein-solubility","display_name":"protein solubility","score":0.8690000176429749},{"id":"https://openalex.org/keywords/heat-shock-proteins","display_name":"heat shock proteins","score":0.6980000138282776},{"id":"https://openalex.org/keywords/culture-temperature","display_name":"culture temperature","score":0.8059999942779541},{"id":"https://openalex.org/keywords/escherichia-coli-expression","display_name":"Escherichia coli expression","score":0.8529999852180481}],"concepts":[{"id":"https://openalex.org/C90559484","wikidata":"https://www.wikidata.org/wiki/Q778379","display_name":"Expression (computer science)","level":2,"score":0.5579320788383484},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.5163879990577698},{"id":"https://openalex.org/C2778348673","wikidata":"https://www.wikidata.org/wiki/Q739302","display_name":"Production (economics)","level":2,"score":0.4743596017360687},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.4444175958633423},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3996402621269226},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.39799076318740845},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.37869757413864136},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.26439934968948364},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.17753902077674866},{"id":"https://openalex.org/C162324750","wikidata":"https://www.wikidata.org/wiki/Q8134","display_name":"Economics","level":0,"score":0.06961983442306519},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0},{"id":"https://openalex.org/C139719470","wikidata":"https://www.wikidata.org/wiki/Q39680","display_name":"Macroeconomics","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1134/s000368382460444x","doi":"https://doi.org/10.1134/s000368382460444x","is_oa":false,"landing_page_url":"https://doi.org/10.1134/s000368382460444x","pdf_url":null,"source":{"id":"https://openalex.org/S37200030","display_name":"Applied Biochemistry and Microbiology","issn_l":"0003-6838","issn":["0003-6838","1608-3024","3034-574X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","jufo-1","norway-1","poland-20","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320267","host_organization_name":"Pleiades Publishing","host_organization_lineage":["https://openalex.org/P4310320267","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Pleiades Publishing","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Biochemistry and Microbiology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.4043000042438507,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":15,"referenced_works":["https://openalex.org/W1255199803","https://openalex.org/W1967385260","https://openalex.org/W2051956161","https://openalex.org/W2077285487","https://openalex.org/W2080434522","https://openalex.org/W2106246537","https://openalex.org/W2160029665","https://openalex.org/W2314225431","https://openalex.org/W2606560528","https://openalex.org/W3119984944","https://openalex.org/W3207325373","https://openalex.org/W4211024409","https://openalex.org/W4211112463","https://openalex.org/W4235506047","https://openalex.org/W4315628248"],"related_works":["https://openalex.org/W4211112463","https://openalex.org/W2592567520","https://openalex.org/W2883214122","https://openalex.org/W2318090358","https://openalex.org/W2984904569","https://openalex.org/W4417427950","https://openalex.org/W2040619357","https://openalex.org/W2367916724","https://openalex.org/W4315628248","https://openalex.org/W3160595304"],"abstract_inverted_index":{"Abstract":[0],"CRM197":[1,50,67],"(Cross":[2],"Reacting":[3],"Material":[4],"197)":[5],"is":[6,39],"an":[7,43,129,142],"inactive":[8],"form":[9,83],"of":[10,25,35,51,84,92,95,111,118,135,137,146,154,179,196,204],"the":[11,21,33,52,82,89,93,98,116,119,122,132,155,159,168,173,191,194,202],"C.":[12],"diphtheriae":[13],"exotoxin":[14],"used":[15],"as":[16,68],"a":[17,60,69,109,177,197],"carrier":[18],"protein":[19,125,170],"for":[20,193,201],"development":[22,34,195],"and":[23,29,45,103,115],"production":[24,203],"conjugated":[26],"polysaccharide":[27],"vaccines":[28],"immunotherapeutic":[30],"drugs.":[31],"However,":[32],"these":[36],"research":[37],"areas":[38],"not":[40],"possible":[41],"without":[42],"efficient":[44,62],"cost-effective":[46],"technology":[47,200],"to":[48,64,166],"produce":[49,65],"proper":[53],"quality.":[54],"In":[55],"this":[56],"study,":[57],"we":[58,127],"developed":[59],"highly":[61],"method":[63],"recombinant":[66],"fusion":[70],"with":[71,141,158,176],"SUMO":[72],"protein,":[73],"yielding":[74],"more":[75,138,147,180],"than":[76,139,148,181],"three":[77],"grams":[78,150,183],"per":[79,151,184],"liter":[80],"in":[81,131,172],"inclusion":[85],"bodies.":[86],"We":[87],"examined":[88],"significant":[90],"effect":[91],"type":[94],"expression":[96,101],"vector,":[97],"heterologous":[99],"gene":[100,120,157,163],"conditions,":[102],"cultivation":[104,113],"on":[105],"its":[106],"solubility.":[107],"Using":[108],"combination":[110],"reduced":[112],"temperature":[114],"promoter":[117],"encoding":[121],"heat":[123],"shock":[124],"CspA,":[126],"achieved":[128],"increase":[130],"solubility":[133],"level":[134,145],"SUMO-CRM197":[136],"30%,":[140],"overall":[143],"biosynthesis":[144],"two":[149],"liter.":[152,185],"Coexpression":[153],"target":[156,169],"DsbC":[160],"disulfide":[161],"isomerase":[162],"allowed":[164],"us":[165],"obtain":[167],"completely":[171],"soluble":[174],"state":[175],"yield":[178],"1.4":[182],"The":[186],"results":[187],"obtained":[188],"may":[189],"become":[190],"basis":[192],"promising":[198],"domestic":[199],"CRM197.":[205]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2024-07-31T00:00:00"}
