{"id":"https://openalex.org/W4399809339","doi":"https://doi.org/10.1038/s41598-024-64767-1","title":"RNASeq highlights ATF6 pathway regulators for CHO cell engineering with different impacts of ATF6\u03b2 and WFS1 knockdown on fed-batch production of IgG1","display_name":"RNASeq highlights ATF6 pathway regulators for CHO cell engineering with different impacts of ATF6\u03b2 and WFS1 knockdown on fed-batch production of IgG1","publication_year":2024,"publication_date":"2024-06-19","ids":{"openalex":"https://openalex.org/W4399809339","doi":"https://doi.org/10.1038/s41598-024-64767-1","pmid":"https://pubmed.ncbi.nlm.nih.gov/38898154","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/11187196"},"language":"en","primary_location":{"id":"doi:10.1038/s41598-024-64767-1","doi":"https://doi.org/10.1038/s41598-024-64767-1","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-024-64767-1","pdf_url":null,"source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1038/s41598-024-64767-1","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5047625661","display_name":"Dyllan Rives","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I8078737","display_name":"Clemson University","ror":"https://ror.org/037s24f05","country_code":"US","type":"education","lineage":["https://openalex.org/I8078737"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Dyllan Rives","raw_affiliation_strings":["Department of Chemical & Biomolecular Engineering, Clemson University, 206 S. Palmetto Blvd., Clemson, SC, 29634-0909, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical & Biomolecular Engineering, Clemson University, 206 S. Palmetto Blvd., Clemson, SC, 29634-0909, USA","institution_ids":["https://openalex.org/I8078737"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5092558990","display_name":"Caroline Peak","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I8078737","display_name":"Clemson University","ror":"https://ror.org/037s24f05","country_code":"US","type":"education","lineage":["https://openalex.org/I8078737"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Caroline Peak","raw_affiliation_strings":["Department of Chemical & Biomolecular Engineering, Clemson University, 206 S. Palmetto Blvd., Clemson, SC, 29634-0909, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical & Biomolecular Engineering, Clemson University, 206 S. Palmetto Blvd., Clemson, SC, 29634-0909, USA","institution_ids":["https://openalex.org/I8078737"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5006285317","display_name":"Mark Blenner","orcid":"https://orcid.org/0000-0001-9274-3749","observed_orcids":["https://orcid.org/0000-0001-9274-3749"]},"institutions":[{"id":"https://openalex.org/I8078737","display_name":"Clemson University","ror":"https://ror.org/037s24f05","country_code":"US","type":"education","lineage":["https://openalex.org/I8078737"]},{"id":"https://openalex.org/I86501945","display_name":"University of Delaware","ror":"https://ror.org/01sbq1a82","country_code":"US","type":"education","lineage":["https://openalex.org/I86501945"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Mark A. Blenner","raw_affiliation_strings":["Department of Chemical & Biomolecular Engineering, Clemson University, 206 S. Palmetto Blvd., Clemson, SC, 29634-0909, USA. blenner@udel.edu","Department of Chemical & Biomolecular Engineering, University of Delaware, 590 Avenue 1743, Newark, DE, 19713, USA. blenner@udel.edu","Department of Chemical & Biomolecular Engineering, Clemson University, 206 S. Palmetto Blvd., Clemson, SC, 29634-0909, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemical & Biomolecular Engineering, Clemson University, 206 S. Palmetto Blvd., Clemson, SC, 29634-0909, USA. blenner@udel.edu","institution_ids":["https://openalex.org/I8078737"]},{"raw_affiliation_string":"Department of Chemical & Biomolecular Engineering, University of Delaware, 590 Avenue 1743, Newark, DE, 19713, USA. blenner@udel.edu","institution_ids":["https://openalex.org/I86501945"]},{"raw_affiliation_string":"Department of Chemical & Biomolecular Engineering, Clemson University, 206 S. Palmetto Blvd., Clemson, SC, 29634-0909, USA","institution_ids":["https://openalex.org/I8078737"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5006285317"],"corresponding_institution_ids":["https://openalex.org/I8078737","https://openalex.org/I86501945"],"apc_list":{"value":2590,"currency":"USD","value_usd":2590},"apc_paid":{"value":2590,"currency":"USD","value_usd":2590},"fwci":2.756,"has_fulltext":true,"cited_by_count":13,"citation_normalized_percentile":{"value":0.90869842,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":98,"max":99},"biblio":{"volume":"14","issue":"1","first_page":"14141","last_page":"14141"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11150","display_name":"Endoplasmic Reticulum Stress and Disease","score":0.6654999852180481,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11150","display_name":"Endoplasmic Reticulum Stress and Disease","score":0.6654999852180481,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.33180001378059387,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/wfs1","display_name":"WFS1","score":0.9980000257492065},{"id":"https://openalex.org/keywords/unfolded-protein-response","display_name":"unfolded protein response","score":0.9670000076293945},{"id":"https://openalex.org/keywords/endoplasmic-reticulum-stress","display_name":"endoplasmic reticulum stress","score":0.9369999766349792},{"id":"https://openalex.org/keywords/cho-cell-engineering","display_name":"CHO cell engineering","score":0.8050000071525574},{"id":"https://openalex.org/keywords/fed-batch-fermentation","display_name":"fed-batch fermentation","score":0.8859999775886536},{"id":"https://openalex.org/keywords/atf6alpha","display_name":"ATF6\u03b1","score":0.6100000143051147},{"id":"https://openalex.org/keywords/er-chaperones","display_name":"ER chaperones","score":0.699999988079071},{"id":"https://openalex.org/keywords/differential-gene-expression","display_name":"differential gene expression","score":0.6710000038146973},{"id":"https://openalex.org/keywords/recombinant-antibody-production","display_name":"recombinant antibody production","score":0.5759999752044678}],"concepts":[{"id":"https://openalex.org/C173396325","wikidata":"https://www.wikidata.org/wiki/Q1501275","display_name":"Gene knockdown","level":3,"score":0.7435240745544434},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.5138676166534424},{"id":"https://openalex.org/C2778997996","wikidata":"https://www.wikidata.org/wiki/Q18036598","display_name":"ATF6","level":4,"score":0.4845077395439148},{"id":"https://openalex.org/C2778348673","wikidata":"https://www.wikidata.org/wiki/Q739302","display_name":"Production (economics)","level":2,"score":0.4559992849826813},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.4063730239868164},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.38313764333724976},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.32613563537597656},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.19576847553253174},{"id":"https://openalex.org/C158617107","wikidata":"https://www.wikidata.org/wiki/Q79927","display_name":"Endoplasmic reticulum","level":2,"score":0.12941691279411316},{"id":"https://openalex.org/C139447449","wikidata":"https://www.wikidata.org/wiki/Q14633900","display_name":"Unfolded protein response","level":3,"score":0.11868453025817871},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.10836362838745117},{"id":"https://openalex.org/C162324750","wikidata":"https://www.wikidata.org/wiki/Q8134","display_name":"Economics","level":0,"score":0.0},{"id":"https://openalex.org/C139719470","wikidata":"https://www.wikidata.org/wiki/Q39680","display_name":"Macroeconomics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003412","descriptor_name":"Cricetulus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D007074","descriptor_name":"Immunoglobulin G","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D007074","descriptor_name":"Immunoglobulin G","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016466","descriptor_name":"CHO Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051702","descriptor_name":"Activating Transcription Factor 6","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D051702","descriptor_name":"Activating Transcription Factor 6","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D055785","descriptor_name":"Gene Knockdown Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D056811","descriptor_name":"Unfolded Protein Response","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D059865","descriptor_name":"Endoplasmic Reticulum Stress","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D060846","descriptor_name":"Cell Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D061249","descriptor_name":"Batch Cell Culture Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1038/s41598-024-64767-1","doi":"https://doi.org/10.1038/s41598-024-64767-1","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-024-64767-1","pdf_url":null,"source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:11187196","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11187196","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11187196/pdf/41598_2024_Article_64767.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Sci Rep","raw_type":"Text"},{"id":"pmid:38898154","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/38898154","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific reports","raw_type":"Journal Article"},{"id":"pmh:oai:udspace.udel.edu:19716/34569","doi":null,"is_oa":true,"landing_page_url":"https://udspace.udel.edu/handle/19716/34569","pdf_url":"https://udspace.udel.edu/bitstreams/6cd470dc-a43a-4d77-898c-be2651254f35/download","source":{"id":"https://openalex.org/S4377196565","display_name":"Library, Museums and Press - UDSpace (University of Delaware)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I86501945","host_organization_name":"University of Delaware","host_organization_lineage":["https://openalex.org/I86501945"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Article"},{"id":"pmh:oai:doaj.org/article:6b563b1546a6477e980be76921b2d10c","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/6b563b1546a6477e980be76921b2d10c","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Scientific Reports, Vol 14, Iss 1, Pp 1-18 (2024)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1038/s41598-024-64767-1","doi":"https://doi.org/10.1038/s41598-024-64767-1","is_oa":true,"landing_page_url":"https://doi.org/10.1038/s41598-024-64767-1","pdf_url":null,"source":{"id":"https://openalex.org/S196734849","display_name":"Scientific Reports","issn_l":"2045-2322","issn":["2045-2322"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310319908","host_organization_name":"Nature Portfolio","host_organization_lineage":["https://openalex.org/P4310319908","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Nature Portfolio","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Scientific Reports","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.5683000087738037}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1884235271","display_name":"UNS:Intergrating novel nutrient feeding strategies with computational glycosylation models to improve production of complex biotherapeutics from mammalian factories","funder_award_id":"1512265","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G2399695739","display_name":"Phase I I/UCRC Johns Hopkins University Site: Advanced Mammalian Biomanufacturing Innovation Center (AMBIC)","funder_award_id":"1624684","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320337390","display_name":"Division of Chemical, Bioengineering, Environmental, and Transport Systems","ror":"https://ror.org/0471zv972"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4399809339.pdf","grobid_xml":"https://content.openalex.org/works/W4399809339.grobid-xml"},"referenced_works_count":95,"referenced_works":["https://openalex.org/W1483951519","https://openalex.org/W1555361166","https://openalex.org/W1688835773","https://openalex.org/W1841746101","https://openalex.org/W1966913704","https://openalex.org/W1966927202","https://openalex.org/W1970090817","https://openalex.org/W1979733074","https://openalex.org/W1979759452","https://openalex.org/W1992604276","https://openalex.org/W1992748149","https://openalex.org/W1994772574","https://openalex.org/W1995041255","https://openalex.org/W1995562003","https://openalex.org/W2003209357","https://openalex.org/W2006260390","https://openalex.org/W2012726787","https://openalex.org/W2017355698","https://openalex.org/W2019192392","https://openalex.org/W2019613871","https://openalex.org/W2024414871","https://openalex.org/W2024753771","https://openalex.org/W2026209245","https://openalex.org/W2027490065","https://openalex.org/W2031735388","https://openalex.org/W2044920551","https://openalex.org/W2045267309","https://openalex.org/W2048086508","https://openalex.org/W2072339970","https://openalex.org/W2081132917","https://openalex.org/W2085856131","https://openalex.org/W2102493845","https://openalex.org/W2104988047","https://openalex.org/W2105405279","https://openalex.org/W2107277218","https://openalex.org/W2112902148","https://openalex.org/W2114360920","https://openalex.org/W2116657339","https://openalex.org/W2124121571","https://openalex.org/W2127906377","https://openalex.org/W2131210945","https://openalex.org/W2131271579","https://openalex.org/W2133003674","https://openalex.org/W2134526812","https://openalex.org/W2134941735","https://openalex.org/W2142850961","https://openalex.org/W2152709023","https://openalex.org/W2159898699","https://openalex.org/W2160301335","https://openalex.org/W2169456326","https://openalex.org/W2170725518","https://openalex.org/W2171228273","https://openalex.org/W2179438025","https://openalex.org/W2273999377","https://openalex.org/W2300282152","https://openalex.org/W2508827573","https://openalex.org/W2560134062","https://openalex.org/W2560195968","https://openalex.org/W2587557922","https://openalex.org/W2593194658","https://openalex.org/W2593546410","https://openalex.org/W2737895222","https://openalex.org/W2740874620","https://openalex.org/W2746989955","https://openalex.org/W2766900039","https://openalex.org/W2782858134","https://openalex.org/W2783150583","https://openalex.org/W2783701314","https://openalex.org/W2790262422","https://openalex.org/W2793350909","https://openalex.org/W2793445127","https://openalex.org/W2796227197","https://openalex.org/W2799744227","https://openalex.org/W2800755544","https://openalex.org/W2802411161","https://openalex.org/W2805107963","https://openalex.org/W2805436547","https://openalex.org/W2898614541","https://openalex.org/W2901990916","https://openalex.org/W2928209680","https://openalex.org/W2943593436","https://openalex.org/W2946114892","https://openalex.org/W2982073644","https://openalex.org/W3006342576","https://openalex.org/W3012203039","https://openalex.org/W3048397665","https://openalex.org/W3092388179","https://openalex.org/W3098260096","https://openalex.org/W3126528616","https://openalex.org/W3157936766","https://openalex.org/W3180737271","https://openalex.org/W4213267976","https://openalex.org/W4241385219","https://openalex.org/W4282918807","https://openalex.org/W4294991486"],"related_works":["https://openalex.org/W7024101143","https://openalex.org/W2587557922","https://openalex.org/W2045267309","https://openalex.org/W4412711596","https://openalex.org/W3016013842","https://openalex.org/W2783150583","https://openalex.org/W4376121499","https://openalex.org/W2578480980","https://openalex.org/W2412770778","https://openalex.org/W3002227902"],"abstract_inverted_index":{"Abstract":[0],"Secretion":[1],"levels":[2],"required":[3],"of":[4,24,50,58,71,111,136,143,156,160,186,202,212,235,247,253,266],"industrial":[5],"Chinese":[6],"hamster":[7],"ovary":[8],"(CHO)":[9],"cell":[10,56,85,113,131,198,205],"lines":[11],"can":[12,27,53],"challenge":[13],"endoplasmic":[14],"reticulum":[15],"(ER)":[16],"homeostasis,":[17],"and":[18,48,68,99,133,164,173,230,245,261],"ER":[19,46,108,248],"stress":[20,109],"caused":[21],"by":[22,222],"accumulation":[23],"misfolded":[25],"proteins":[26],"be":[28],"a":[29],"bottleneck":[30],"in":[31,43,122,147,192,237,273],"biomanufacturing.":[32],"The":[33,118],"unfolded":[34],"protein":[35],"response":[36,44,70,110],"(UPR)":[37],"is":[38],"initiated":[39],"to":[40,45,80,105,128,178,276],"restore":[41],"homeostasis":[42],"stress,":[47],"optimization":[49],"the":[51,63,107,123,129,148,157,161,269],"UPR":[52,119,259,270],"improve":[54],"CHO":[55,197],"production":[57,66],"therapeutic":[59],"proteins.":[60],"We":[61,87],"compared":[62,127],"fed-batch":[64,116,274],"growth,":[65],"characteristics,":[67],"transcriptomic":[69],"an":[72,193],"immunoglobulin":[73],"G":[74],"1":[75,77,125,150,195],"(IgG":[76],")":[78],"producer":[79,126],"its":[81],"parental,":[82],"non-producing":[83],"host":[84,130],"line.":[86,199],"conducted":[88],"differential":[89,137],"gene":[90],"expression":[91,138,220],"analysis":[92,135],"using":[93],"high":[94],"throughput":[95],"RNA":[96],"sequencing":[97],"(RNASeq)":[98],"quantitative":[100],"polymerase":[101],"chain":[102],"reaction":[103],"(qPCR)":[104],"study":[106,183],"each":[112],"line":[114,132],"during":[115],"culture.":[117],"was":[120,240],"activated":[121],"IgG":[124,149,194],"our":[134],"profiles":[139],"indicated":[140,224],"transient":[141],"upregulation":[142,234,246],"ATF6\u03b1":[144],"target":[145,188],"mRNAs":[146],"producer,":[151],"suggesting":[152],"two":[153],"upstream":[154],"regulators":[155],"ATF6":[158],"arm":[159],"UPR,":[162],"ATF6\u03b2":[163,172,203,229,267],"WFS1,":[165],"are":[166],"rational":[167],"engineering":[168],"targets.":[169],"Although":[170],"both":[171],"WFS1":[174,213,231,236,254],"have":[175],"been":[176],"reported":[177],"negatively":[179],"regulate":[180],"ATF6\u03b1,":[181],"this":[182],"shows":[184],"knockdown":[185,201,211,252,265],"either":[187],"elicits":[189],"different":[190],"effects":[191],"-producing":[196],"Stable":[200],"decreased":[204,214,243],"growth":[206,244],"without":[207,216],"decreasing":[208],"titer;":[209],"however,":[210],"titer":[215],"affecting":[217],"growth.":[218],"Relative":[219],"measured":[221],"qPCR":[223],"no":[225],"direct":[226],"relationship":[227],"between":[228],"expression,":[232,264],"but":[233],"one":[238],"pool":[239],"correlated":[241],"with":[242],"chaperone":[249],"mRNAs.":[250],"While":[251],"had":[255],"negative":[256],"impacts":[257],"on":[258],"activation":[260],"product":[262],"mRNA":[263],"improved":[268],"specifically":[271],"later":[272],"leading":[275],"increased":[277],"overall":[278],"productivity.":[279]},"counts_by_year":[{"year":2026,"cited_by_count":8},{"year":2025,"cited_by_count":5}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2024-06-20T00:00:00"}