{"id":"https://openalex.org/W4399126403","doi":"https://doi.org/10.1093/ismeco/ycae075","title":"Hackflex library preparation enables low-cost metagenomic profiling","display_name":"Hackflex library preparation enables low-cost metagenomic profiling","publication_year":2024,"publication_date":"2024-01-01","ids":{"openalex":"https://openalex.org/W4399126403","doi":"https://doi.org/10.1093/ismeco/ycae075","pmid":"https://pubmed.ncbi.nlm.nih.gov/38712258","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/11071439"},"language":"en","primary_location":{"id":"doi:10.1093/ismeco/ycae075","doi":"https://doi.org/10.1093/ismeco/ycae075","is_oa":true,"landing_page_url":"https://doi.org/10.1093/ismeco/ycae075","pdf_url":null,"source":{"id":"https://openalex.org/S4210240812","display_name":"ISME Communications","issn_l":"2730-6151","issn":["2730-6151"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-1","norway-1","oarl","vabb-shw"],"host_organization":"https://openalex.org/P4310319965","host_organization_name":"Springer Nature","host_organization_lineage":["https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ISME Communications","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1093/ismeco/ycae075","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5050326612","display_name":"Samantha L. Goldman","orcid":"https://orcid.org/0000-0001-9577-6626","observed_orcids":["https://orcid.org/0000-0001-9577-6626"]},"institutions":[{"id":"https://openalex.org/I20089843","display_name":"Princeton University","ror":"https://ror.org/00hx57361","country_code":"US","type":"education","lineage":["https://openalex.org/I20089843"]},{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Samantha L Goldman","raw_affiliation_strings":["Department of Ecology and Evolutionary Biology, Cornell University , 215 Tower Rd, Ithaca, NY 14850 , United States","Department of Ecology and Evolutionary Biology, Princeton University , 301 Guyot, Princeton, NJ 08540 , United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ecology and Evolutionary Biology, Cornell University , 215 Tower Rd, Ithaca, NY 14850 , United States","institution_ids":["https://openalex.org/I205783295"]},{"raw_affiliation_string":"Department of Ecology and Evolutionary Biology, Princeton University , 301 Guyot, Princeton, NJ 08540 , United States","institution_ids":["https://openalex.org/I20089843"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5069073018","display_name":"Jon G. Sanders","orcid":"https://orcid.org/0000-0001-6077-4014","observed_orcids":["https://orcid.org/0000-0001-6077-4014"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jon G Sanders","raw_affiliation_strings":["Department of Ecology and Evolutionary Biology, Cornell University , 215 Tower Rd, Ithaca, NY 14850 , United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ecology and Evolutionary Biology, Cornell University , 215 Tower Rd, Ithaca, NY 14850 , United States","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037232354","display_name":"Daniel D. Sprockett","orcid":"https://orcid.org/0000-0003-2620-7555","observed_orcids":["https://orcid.org/0000-0003-2620-7555"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Daniel D Sprockett","raw_affiliation_strings":["Department of Ecology and Evolutionary Biology, Cornell University , 215 Tower Rd, Ithaca, NY 14850 , United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ecology and Evolutionary Biology, Cornell University , 215 Tower Rd, Ithaca, NY 14850 , United States","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5040576494","display_name":"Abigail A. Landers","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Abigail Landers","raw_affiliation_strings":["Department of Ecology and Evolutionary Biology, Cornell University , 215 Tower Rd, Ithaca, NY 14850 , United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ecology and Evolutionary Biology, Cornell University , 215 Tower Rd, Ithaca, NY 14850 , United States","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055932687","display_name":"Weiwei Yan","orcid":"https://orcid.org/0000-0002-8640-6700","observed_orcids":["https://orcid.org/0000-0002-8640-6700"]},"institutions":[{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Weiwei Yan","raw_affiliation_strings":["Department of Ecology and Evolutionary Biology, Cornell University , 215 Tower Rd, Ithaca, NY 14850 , United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ecology and Evolutionary Biology, Cornell University , 215 Tower Rd, Ithaca, NY 14850 , United States","institution_ids":["https://openalex.org/I205783295"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5039951389","display_name":"Andrew H. Moeller","orcid":"https://orcid.org/0000-0002-8377-4647","observed_orcids":["https://orcid.org/0000-0002-8377-4647"]},"institutions":[{"id":"https://openalex.org/I20089843","display_name":"Princeton University","ror":"https://ror.org/00hx57361","country_code":"US","type":"education","lineage":["https://openalex.org/I20089843"]},{"id":"https://openalex.org/I205783295","display_name":"Cornell University","ror":"https://ror.org/05bnh6r87","country_code":"US","type":"education","lineage":["https://openalex.org/I205783295"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Andrew H Moeller","raw_affiliation_strings":["Department of Ecology and Evolutionary Biology, Cornell University , 215 Tower Rd, Ithaca, NY 14850 , United States","Department of Ecology and Evolutionary Biology, Princeton University , 301 Guyot, Princeton, NJ 08540 , United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Ecology and Evolutionary Biology, Cornell University , 215 Tower Rd, Ithaca, NY 14850 , United States","institution_ids":["https://openalex.org/I205783295"]},{"raw_affiliation_string":"Department of Ecology and Evolutionary Biology, Princeton University , 301 Guyot, Princeton, NJ 08540 , United States","institution_ids":["https://openalex.org/I20089843"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5039951389","https://openalex.org/A5050326612"],"corresponding_institution_ids":["https://openalex.org/I20089843","https://openalex.org/I205783295"],"apc_list":{"value":2426,"currency":"EUR","value_usd":2625},"apc_paid":{"value":2426,"currency":"EUR","value_usd":2625},"fwci":0.3881,"has_fulltext":true,"cited_by_count":3,"citation_normalized_percentile":{"value":0.50302662,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":97,"max":98},"biblio":{"volume":"4","issue":"1","first_page":"ycae075","last_page":"ycae075"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.9477999806404114,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.9477999806404114,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10066","display_name":"Gut microbiota and health","score":0.02630000002682209,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.011900000274181366,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/low-cost-sequencing","display_name":"low-cost sequencing","score":0.7799999713897705},{"id":"https://openalex.org/keywords/mock-microbial-community","display_name":"mock microbial community","score":0.5799999833106995},{"id":"https://openalex.org/keywords/inbred-mouse-strains","display_name":"inbred mouse strains","score":0.7760000228881836},{"id":"https://openalex.org/keywords/bacterial-community-composition","display_name":"bacterial community composition","score":0.675000011920929},{"id":"https://openalex.org/keywords/sequencing-cost-reduction","display_name":"sequencing cost reduction","score":0.4309999942779541}],"concepts":[{"id":"https://openalex.org/C15151743","wikidata":"https://www.wikidata.org/wiki/Q903778","display_name":"Metagenomics","level":3,"score":0.9658243656158447},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6128532886505127},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5908730030059814},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.570292055606842},{"id":"https://openalex.org/C101985253","wikidata":"https://www.wikidata.org/wiki/Q1073526","display_name":"Shotgun sequencing","level":4,"score":0.49846768379211426},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.32313811779022217},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2585690915584564},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.20755130052566528},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1390063464641571}],"mesh":[],"locations_count":6,"locations":[{"id":"doi:10.1093/ismeco/ycae075","doi":"https://doi.org/10.1093/ismeco/ycae075","is_oa":true,"landing_page_url":"https://doi.org/10.1093/ismeco/ycae075","pdf_url":null,"source":{"id":"https://openalex.org/S4210240812","display_name":"ISME Communications","issn_l":"2730-6151","issn":["2730-6151"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-1","norway-1","oarl","vabb-shw"],"host_organization":"https://openalex.org/P4310319965","host_organization_name":"Springer Nature","host_organization_lineage":["https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ISME Communications","raw_type":"journal-article"},{"id":"doi:10.1101/2024.04.23.590092","doi":"https://doi.org/10.1101/2024.04.23.590092","is_oa":true,"landing_page_url":"https://doi.org/10.1101/2024.04.23.590092","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2024/04/23/2024.04.23.590092.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmh:oai:pubmedcentral.nih.gov:11190725","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11190725","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11190725/pdf/ycae075.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ISME Commun","raw_type":"Text"},{"id":"pmid:38712258","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/38712258","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"bioRxiv : the preprint server for biology","raw_type":"Preprint"},{"id":"pmid:38912052","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/38912052","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ISME communications","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:11071439","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11071439","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11071439/pdf/nihpp-2024.04.23.590092v1.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"Text"}],"best_oa_location":{"id":"doi:10.1093/ismeco/ycae075","doi":"https://doi.org/10.1093/ismeco/ycae075","is_oa":true,"landing_page_url":"https://doi.org/10.1093/ismeco/ycae075","pdf_url":null,"source":{"id":"https://openalex.org/S4210240812","display_name":"ISME Communications","issn_l":"2730-6151","issn":["2730-6151"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-d","jufo-1","norway-1","oarl","vabb-shw"],"host_organization":"https://openalex.org/P4310319965","host_organization_name":"Springer Nature","host_organization_lineage":["https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ISME Communications","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1436913737","display_name":"Host-Microbe Interactions that Determine Host Traits and Disease","funder_award_id":"T32AI145821","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G2403277600","display_name":"Uncovering the modes and genetics of gut bacterial transmission","funder_award_id":"R35GM138284","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320313550","display_name":"Society for the Study of Evolution","ror":"https://ror.org/057kr0a20"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4399126403.pdf","grobid_xml":"https://content.openalex.org/works/W4399126403.grobid-xml"},"referenced_works_count":21,"referenced_works":["https://openalex.org/W2136678168","https://openalex.org/W2789843538","https://openalex.org/W2797794199","https://openalex.org/W2914348147","https://openalex.org/W2990618091","https://openalex.org/W3047400193","https://openalex.org/W3110491465","https://openalex.org/W3163198128","https://openalex.org/W3164517331","https://openalex.org/W3173669103","https://openalex.org/W3192642189","https://openalex.org/W3198597471","https://openalex.org/W3206629621","https://openalex.org/W3211927632","https://openalex.org/W3213970691","https://openalex.org/W3214104032","https://openalex.org/W4205280965","https://openalex.org/W4224943843","https://openalex.org/W4282000167","https://openalex.org/W4283461083","https://openalex.org/W4304692376"],"related_works":["https://openalex.org/W3154437000","https://openalex.org/W7221380055","https://openalex.org/W4205280965","https://openalex.org/W2974299855","https://openalex.org/W3170803369","https://openalex.org/W1863739124","https://openalex.org/W4220737584","https://openalex.org/W7097813300","https://openalex.org/W7136571382","https://openalex.org/W4255150620"],"abstract_inverted_index":{"Shotgun":[0],"metagenomic":[1,51,60,137,158],"sequencing":[2,59],"provides":[3],"valuable":[4],"insights":[5],"into":[6],"microbial":[7],"communities,":[8],"but":[9],"the":[10,88,113,118,134],"high":[11],"cost":[12],"of":[13,87,108,133,154],"library":[14,39,141],"preparation":[15,40,142],"with":[16,96],"standard":[17],"kits":[18],"and":[19,78,122],"protocols":[20],"is":[21,149],"a":[22,130],"barrier":[23],"for":[24,50,94,151],"many.":[25],"New":[26],"methods":[27,44],"such":[28],"as":[29,66,68],"Hackflex":[30,56,82,102,148],"use":[31],"diluted":[32],"commercially":[33],"available":[34],"reagents":[35],"to":[36,105],"greatly":[37],"reduce":[38],"costs.":[41],"However,":[42],"these":[43],"have":[45],"not":[46],"been":[47],"systematically":[48],"validated":[49],"sequencing.":[52,159],"Here,":[53],"we":[54],"evaluate":[55],"performance":[57],"by":[58,73],"libraries":[61],"from":[62,112],"known":[63],"mock":[64,90],"communities":[65,156],"well":[67],"mouse":[69,120,127],"fecal":[70],"samples":[71,95],"prepared":[72],"Hackflex,":[74],"Illumina":[75,79],"DNA":[76,97],"Prep,":[77],"TruSeq":[80],"methods.":[81],"successfully":[83],"recovered":[84],"all":[85],"members":[86],"Zymo":[89],"community,":[91],"performing":[92],"best":[93],"concentrations":[98],"<1":[99],"ng/uL.":[100],"Furthermore,":[101],"was":[103],"able":[104],"delineate":[106],"microbiota":[107],"individual":[109],"inbred":[110],"mice":[111],"same":[114,119],"breeding":[115],"stock":[116],"at":[117],"facility,":[121],"statistical":[123],"modeling":[124],"indicated":[125],"that":[126,147],"ID":[128],"explained":[129],"greater":[131],"fraction":[132],"variance":[135],"in":[136],"composition":[138],"than":[139],"did":[140],"method.":[143],"These":[144],"results":[145],"show":[146],"suitable":[150],"generating":[152],"inventories":[153],"bacterial":[155],"through":[157]},"counts_by_year":[{"year":2026,"cited_by_count":3}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2024-05-30T00:00:00"}
