{"id":"https://openalex.org/W4398205403","doi":"https://doi.org/10.3390/jcm13113028","title":"Good Manufacturing Practice-Compliant Cryopreserved and Thawed Native Adipose Tissue Ready for Fat Grafting","display_name":"Good Manufacturing Practice-Compliant Cryopreserved and Thawed Native Adipose Tissue Ready for Fat Grafting","publication_year":2024,"publication_date":"2024-05-21","ids":{"openalex":"https://openalex.org/W4398205403","doi":"https://doi.org/10.3390/jcm13113028","pmid":"https://pubmed.ncbi.nlm.nih.gov/38892739","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/11172459"},"language":"en","primary_location":{"id":"doi:10.3390/jcm13113028","doi":"https://doi.org/10.3390/jcm13113028","is_oa":true,"landing_page_url":"https://doi.org/10.3390/jcm13113028","pdf_url":null,"source":{"id":"https://openalex.org/S2737969411","display_name":"Journal of Clinical Medicine","issn_l":"2077-0383","issn":["2077-0383"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","ki-jl-1","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Medicine","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.3390/jcm13113028","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085040906","display_name":"Giulio Rusconi","orcid":"https://orcid.org/0000-0002-0987-042X","observed_orcids":["https://orcid.org/0000-0002-0987-042X"]},"institutions":[{"id":"https://openalex.org/I4210134281","display_name":"Swiss Stem Cell Foundation","ror":"https://ror.org/03sf5ja42","country_code":"CH","type":"nonprofit","lineage":["https://openalex.org/I4210134281"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Giulio Rusconi","raw_affiliation_strings":["Swiss Stem Cell Foundation, 6900 Lugano, Switzerland"],"raw_orcid":"https://orcid.org/0000-0002-0987-042X","affiliations":[{"raw_affiliation_string":"Swiss Stem Cell Foundation, 6900 Lugano, Switzerland","institution_ids":["https://openalex.org/I4210134281"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046025479","display_name":"Martina Cremona","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210134281","display_name":"Swiss Stem Cell Foundation","ror":"https://ror.org/03sf5ja42","country_code":"CH","type":"nonprofit","lineage":["https://openalex.org/I4210134281"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Martina Cremona","raw_affiliation_strings":["Swiss Stem Cell Foundation, 6900 Lugano, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Swiss Stem Cell Foundation, 6900 Lugano, Switzerland","institution_ids":["https://openalex.org/I4210134281"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009460285","display_name":"Matteo Gallazzi","orcid":"https://orcid.org/0000-0001-6662-9352","observed_orcids":["https://orcid.org/0000-0001-6662-9352"]},"institutions":[{"id":"https://openalex.org/I4210134281","display_name":"Swiss Stem Cell Foundation","ror":"https://ror.org/03sf5ja42","country_code":"CH","type":"nonprofit","lineage":["https://openalex.org/I4210134281"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Matteo Gallazzi","raw_affiliation_strings":["Swiss Stem Cell Foundation, 6900 Lugano, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Swiss Stem Cell Foundation, 6900 Lugano, Switzerland","institution_ids":["https://openalex.org/I4210134281"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5029900336","display_name":"Luca Mariotta","orcid":"https://orcid.org/0000-0002-0456-3885","observed_orcids":["https://orcid.org/0000-0002-0456-3885"]},"institutions":[{"id":"https://openalex.org/I4210134281","display_name":"Swiss Stem Cell Foundation","ror":"https://ror.org/03sf5ja42","country_code":"CH","type":"nonprofit","lineage":["https://openalex.org/I4210134281"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Luca Mariotta","raw_affiliation_strings":["Swiss Stem Cell Foundation, 6900 Lugano, Switzerland","Swiss Stem Cells Biotech AG, 8008 Zurich, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Swiss Stem Cell Foundation, 6900 Lugano, Switzerland","institution_ids":["https://openalex.org/I4210134281"]},{"raw_affiliation_string":"Swiss Stem Cells Biotech AG, 8008 Zurich, Switzerland","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038976158","display_name":"Mauro Gola","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210134281","display_name":"Swiss Stem Cell Foundation","ror":"https://ror.org/03sf5ja42","country_code":"CH","type":"nonprofit","lineage":["https://openalex.org/I4210134281"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Mauro Gola","raw_affiliation_strings":["Swiss Stem Cell Foundation, 6900 Lugano, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Swiss Stem Cell Foundation, 6900 Lugano, Switzerland","institution_ids":["https://openalex.org/I4210134281"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078599596","display_name":"Eugenio Gandolfi","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Eugenio Gandolfi","raw_affiliation_strings":["Clinica Sant\u2019Anna, Swiss Medical Network, 6924 Sorengo, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Clinica Sant\u2019Anna, Swiss Medical Network, 6924 Sorengo, Switzerland","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"Matteo Malacco","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Matteo Malacco","raw_affiliation_strings":["Clinica Sant\u2019Anna, Swiss Medical Network, 6924 Sorengo, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Clinica Sant\u2019Anna, Swiss Medical Network, 6924 Sorengo, Switzerland","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5043068469","display_name":"Gianni Soldati","orcid":"https://orcid.org/0000-0001-6309-9456","observed_orcids":["https://orcid.org/0000-0001-6309-9456"]},"institutions":[{"id":"https://openalex.org/I4210134281","display_name":"Swiss Stem Cell Foundation","ror":"https://ror.org/03sf5ja42","country_code":"CH","type":"nonprofit","lineage":["https://openalex.org/I4210134281"]}],"countries":["CH"],"is_corresponding":true,"raw_author_name":"Gianni Soldati","raw_affiliation_strings":["Swiss Stem Cell Foundation, 6900 Lugano, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Swiss Stem Cell Foundation, 6900 Lugano, Switzerland","institution_ids":["https://openalex.org/I4210134281"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5043068469"],"corresponding_institution_ids":["https://openalex.org/I4210134281"],"apc_list":{"value":2600,"currency":"CHF","value_usd":2955},"apc_paid":{"value":2600,"currency":"CHF","value_usd":2955},"fwci":0.5693,"has_fulltext":true,"cited_by_count":4,"citation_normalized_percentile":{"value":0.54367767,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":96},"biblio":{"volume":"13","issue":"11","first_page":"3028","last_page":"3028"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.9965999722480774,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11457","display_name":"Adipose Tissue and Metabolism","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/2737","display_name":"Physiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12264","display_name":"Tissue Engineering and Regenerative Medicine","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/good-manufacturing-practices","display_name":"good manufacturing practices","score":0.7450000047683716},{"id":"https://openalex.org/keywords/fat-grafting","display_name":"fat grafting","score":0.7459999918937683},{"id":"https://openalex.org/keywords/stromal-vascular-fraction","display_name":"stromal vascular fraction","score":0.906000018119812},{"id":"https://openalex.org/keywords/adipose-derived-stem-cells","display_name":"adipose-derived stem cells","score":0.9380000233650208},{"id":"https://openalex.org/keywords/cell-viability-loss","display_name":"cell viability loss","score":0.6549999713897705},{"id":"https://openalex.org/keywords/liquid-nitrogen-storage","display_name":"liquid nitrogen storage","score":0.6930000185966492},{"id":"https://openalex.org/keywords/regenerative-medicine","display_name":"regenerative medicine","score":0.9010000228881836}],"concepts":[{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.9346740245819092},{"id":"https://openalex.org/C171089720","wikidata":"https://www.wikidata.org/wiki/Q193583","display_name":"Adipose tissue","level":2,"score":0.8490651249885559},{"id":"https://openalex.org/C179933525","wikidata":"https://www.wikidata.org/wiki/Q1144664","display_name":"Cryopreservation","level":3,"score":0.7640503644943237},{"id":"https://openalex.org/C2775937945","wikidata":"https://www.wikidata.org/wiki/Q222958","display_name":"Grafting","level":3,"score":0.5785858631134033},{"id":"https://openalex.org/C141071460","wikidata":"https://www.wikidata.org/wiki/Q40821","display_name":"Surgery","level":1,"score":0.3246237635612488},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.12878161668777466},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.10612824559211731},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.10257378220558167},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.0},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.0},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.0}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.3390/jcm13113028","doi":"https://doi.org/10.3390/jcm13113028","is_oa":true,"landing_page_url":"https://doi.org/10.3390/jcm13113028","pdf_url":null,"source":{"id":"https://openalex.org/S2737969411","display_name":"Journal of Clinical Medicine","issn_l":"2077-0383","issn":["2077-0383"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","ki-jl-1","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Medicine","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:11172459","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11172459","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11172459/pdf/jcm-13-03028.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Clin Med","raw_type":"Text"},{"id":"pmid:38892739","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/38892739","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of clinical medicine","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.3390/jcm13113028","doi":"https://doi.org/10.3390/jcm13113028","is_oa":true,"landing_page_url":"https://doi.org/10.3390/jcm13113028","pdf_url":null,"source":{"id":"https://openalex.org/S2737969411","display_name":"Journal of Clinical Medicine","issn_l":"2077-0383","issn":["2077-0383"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","ki-jl-1","norway-1","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Medicine","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.9391000270843506,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4398205403.pdf","grobid_xml":"https://content.openalex.org/works/W4398205403.grobid-xml"},"referenced_works_count":22,"referenced_works":["https://openalex.org/W1978330100","https://openalex.org/W1987445901","https://openalex.org/W1989225880","https://openalex.org/W2024683933","https://openalex.org/W2025371271","https://openalex.org/W2027413537","https://openalex.org/W2028485108","https://openalex.org/W2034104567","https://openalex.org/W2046156745","https://openalex.org/W2053925160","https://openalex.org/W2091695136","https://openalex.org/W2113358240","https://openalex.org/W2149567755","https://openalex.org/W2171835251","https://openalex.org/W2558926284","https://openalex.org/W2756376978","https://openalex.org/W2918444259","https://openalex.org/W3139400144","https://openalex.org/W3159820173","https://openalex.org/W3185809634","https://openalex.org/W4225817378","https://openalex.org/W4376630220"],"related_works":["https://openalex.org/W2149567755","https://openalex.org/W7125178537","https://openalex.org/W2756376978","https://openalex.org/W2794154788","https://openalex.org/W7160917238","https://openalex.org/W969720518","https://openalex.org/W4300447618","https://openalex.org/W1978246677","https://openalex.org/W4409086827","https://openalex.org/W4403923797"],"abstract_inverted_index":{"Background:":[0],"As":[1],"adipose":[2,35,69,166,186],"tissue-derived":[3],"mesenchymal":[4],"stem":[5],"cells":[6,141,199],"are":[7,20,150,179],"becoming":[8],"the":[9,29,49,53,143,163,182,213],"tool":[10,47],"of":[11,33,52,57,68,113,135,138,165,198],"choice":[12],"for":[13,48,152,200],"many":[14],"clinical":[15],"applications;":[16],"standardized":[17],"cryopreservation":[18,30,102,164,170],"protocols":[19],"necessary":[21],"to":[22,110,161],"deliver":[23],"high-quality":[24],"samples.":[25,219],"For":[26],"this":[27,64,177],"purpose,":[28],"and":[31,45,55,73,76,92,100,121,147,191,217],"thawing":[32],"native":[34,185],"tissue":[36,70,167],"under":[37],"GMP":[38,148],"conditions":[39],"could":[40,193],"represent":[41],"an":[42],"extremely":[43],"useful":[44,196],"powerful":[46],"direct":[50],"reinfusion":[51],"tissue,":[54,187],"consequently,":[56],"its":[58],"stromal":[59],"vascular":[60],"fraction.":[61],"Methods:":[62],"In":[63],"study,":[65],"19":[66,85],"samples":[67,115,149],"were":[71,88,116],"cryopreserved":[72],"characterized":[74,122],"before":[75],"after":[77],"storage":[78],"in":[79,90,94,176,204,212],"liquid":[80],"nitrogen":[81],"vapors.":[82],"Of":[83],"these":[84],"samples,":[86],"14":[87],"processed":[89,190],"research":[91],"5":[93],"a":[95,133,195,209],"GMP-compliant":[96],"environment.":[97],"Storage":[98],"with":[99,123,181],"without":[101,168],"medium":[103],"was":[104],"also":[105,158],"evaluated.":[106],"After":[107],"one":[108],"week":[109],"three":[111],"months":[112],"storage,":[114],"thawed,":[117],"washed,":[118],"enzymatically":[119],"digested,":[120],"flow":[124],"cytometry.":[125],"Results:":[126],"The":[127,173],"results":[128],"show":[129],"that":[130,184,206],"there":[131,207],"is":[132,208],"loss":[134],"nearly":[136],"50%":[137],"total":[139],"nucleated":[140],"during":[142],"cryopreservation/thawing":[144],"process.":[145],"Non-GMP":[146],"comparable":[151],"all":[153],"parameters":[154],"analyzed.":[155],"This":[156],"study":[157],"allowed":[159],"us":[160],"exclude":[162],"any":[169],"medium.":[171],"Conclusions:":[172],"data":[174],"shown":[175],"work":[178],"consistent":[180],"idea":[183],"if":[188],"properly":[189],"controlled,":[192],"be":[194],"source":[197],"regenerative":[201],"medicine,":[202],"keeping":[203],"mind":[205],"clear":[210],"difference":[211],"quality":[214],"between":[215],"fresh":[216],"thawed":[218]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2024-05-23T00:00:00"}