{"id":"https://openalex.org/W4397046920","doi":"https://doi.org/10.1681/asn.20223311s1814c","title":"Identification of a Mechanosensor in Juxtaglomerular Cells for the Regulation of Renin Synthesis and Secretion","display_name":"Identification of a Mechanosensor in Juxtaglomerular Cells for the Regulation of Renin Synthesis and Secretion","publication_year":2022,"publication_date":"2022-11-01","ids":{"openalex":"https://openalex.org/W4397046920","doi":"https://doi.org/10.1681/asn.20223311s1814c"},"language":"en","primary_location":{"id":"doi:10.1681/asn.20223311s1814c","doi":"https://doi.org/10.1681/asn.20223311s1814c","is_oa":false,"landing_page_url":"https://doi.org/10.1681/asn.20223311s1814c","pdf_url":null,"source":{"id":"https://openalex.org/S115693375","display_name":"Journal of the American Society of Nephrology","issn_l":"1046-6673","issn":["1046-6673","1533-3450"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315620","host_organization_name":"American Society of Nephrology","host_organization_lineage":["https://openalex.org/P4310315620"],"host_organization_lineage_names":["American Society of Nephrology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Society of Nephrology","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5040630385","display_name":"Jin Wei","orcid":"https://orcid.org/0000-0001-8040-8126","observed_orcids":["https://orcid.org/0000-0001-8040-8126"]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jin Wei","raw_affiliation_strings":["USF Health Morsani College of Medicine, Tampa, FL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"USF Health Morsani College of Medicine, Tampa, FL","institution_ids":["https://openalex.org/I2613432"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100436618","display_name":"Jie Zhang","orcid":"https://orcid.org/0000-0001-6717-4118","observed_orcids":["https://orcid.org/0000-0001-6717-4118"]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jie Zhang","raw_affiliation_strings":["USF Health Morsani College of Medicine, Tampa, FL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"USF Health Morsani College of Medicine, Tampa, FL","institution_ids":["https://openalex.org/I2613432"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101786320","display_name":"Nikita Singh Yadav","orcid":"https://orcid.org/0000-0001-7818-0388","observed_orcids":["https://orcid.org/0000-0001-7818-0388"]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nikita S. Yadav","raw_affiliation_strings":["USF Health Morsani College of Medicine, Tampa, FL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"USF Health Morsani College of Medicine, Tampa, FL","institution_ids":["https://openalex.org/I2613432"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045818290","display_name":"J. C. M. Chan","orcid":"https://orcid.org/0009-0000-2285-2042","observed_orcids":["https://orcid.org/0009-0000-2285-2042"]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jenna Chan","raw_affiliation_strings":["USF Health Morsani College of Medicine, Tampa, FL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"USF Health Morsani College of Medicine, Tampa, FL","institution_ids":["https://openalex.org/I2613432"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078203955","display_name":"Kshama Patel","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kshama Patel","raw_affiliation_strings":["USF Health Morsani College of Medicine, Tampa, FL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"USF Health Morsani College of Medicine, Tampa, FL","institution_ids":["https://openalex.org/I2613432"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009906433","display_name":"Anish Thalakola","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Anish R. Thalakola","raw_affiliation_strings":["USF Health Morsani College of Medicine, Tampa, FL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"USF Health Morsani College of Medicine, Tampa, FL","institution_ids":["https://openalex.org/I2613432"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016193327","display_name":"Ridham Patel","orcid":"https://orcid.org/0000-0002-8216-4986","observed_orcids":["https://orcid.org/0000-0002-8216-4986"]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ridham Patel","raw_affiliation_strings":["USF Health Morsani College of Medicine, Tampa, FL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"USF Health Morsani College of Medicine, Tampa, FL","institution_ids":["https://openalex.org/I2613432"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5098690743","display_name":"Fady G. Attalla","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Fady G. Attalla","raw_affiliation_strings":["USF Health Morsani College of Medicine, Tampa, FL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"USF Health Morsani College of Medicine, Tampa, FL","institution_ids":["https://openalex.org/I2613432"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111199823","display_name":"Garrett D. Meredith","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Garrett D. Meredith","raw_affiliation_strings":["USF Health Morsani College of Medicine, Tampa, FL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"USF Health Morsani College of Medicine, Tampa, FL","institution_ids":["https://openalex.org/I2613432"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5045686015","display_name":"Ruisheng Liu","orcid":"https://orcid.org/0000-0002-2220-1888","observed_orcids":["https://orcid.org/0000-0002-2220-1888"]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ruisheng Liu","raw_affiliation_strings":["USF Health Morsani College of Medicine, Tampa, FL"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"USF Health Morsani College of Medicine, Tampa, FL","institution_ids":["https://openalex.org/I2613432"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I2613432"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"33","issue":"11S","first_page":"814","last_page":"814"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11001","display_name":"Renin-Angiotensin System Studies","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11001","display_name":"Renin-Angiotensin System Studies","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12067","display_name":"Renal and Vascular Pathologies","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/2727","display_name":"Nephrology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T14486","display_name":"Cardiovascular, Neuropeptides, and Oxidative Stress Research","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2746","display_name":"Surgery"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/juxtaglomerular-cells","display_name":"juxtaglomerular cells","score":0.9959999918937683},{"id":"https://openalex.org/keywords/renin-secretion","display_name":"renin secretion","score":0.7509999871253967},{"id":"https://openalex.org/keywords/renin-gene-expression","display_name":"renin gene expression","score":0.6079999804496765},{"id":"https://openalex.org/keywords/mechanosensors","display_name":"mechanosensors","score":0.8579999804496765},{"id":"https://openalex.org/keywords/syndecan-1","display_name":"syndecan-1","score":0.8209999799728394},{"id":"https://openalex.org/keywords/perfusion-pressure","display_name":"perfusion pressure","score":0.7369999885559082},{"id":"https://openalex.org/keywords/afferent-arteriole","display_name":"afferent arteriole","score":0.9490000009536743},{"id":"https://openalex.org/keywords/angiotensin-ii","display_name":"angiotensin II","score":0.843999981880188},{"id":"https://openalex.org/keywords/vascular-smooth-muscle-cells","display_name":"vascular smooth muscle cells","score":0.8130000233650208},{"id":"https://openalex.org/keywords/nr2f1","display_name":"NR2F1","score":0.7229999899864197}],"concepts":[{"id":"https://openalex.org/C49039625","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretion","level":2,"score":0.6650182604789734},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.622924268245697},{"id":"https://openalex.org/C198710026","wikidata":"https://www.wikidata.org/wiki/Q898218","display_name":"Renin\u2013angiotensin system","level":3,"score":0.5521501302719116},{"id":"https://openalex.org/C2779078858","wikidata":"https://www.wikidata.org/wiki/Q1358572","display_name":"Juxtaglomerular apparatus","level":4,"score":0.463714599609375},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4272312521934509},{"id":"https://openalex.org/C134018914","wikidata":"https://www.wikidata.org/wiki/Q162606","display_name":"Endocrinology","level":1,"score":0.38353195786476135},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.3530943989753723},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.33274179697036743},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.3256210684776306},{"id":"https://openalex.org/C84393581","wikidata":"https://www.wikidata.org/wiki/Q82642","display_name":"Blood pressure","level":2,"score":0.0},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1681/asn.20223311s1814c","doi":"https://doi.org/10.1681/asn.20223311s1814c","is_oa":false,"landing_page_url":"https://doi.org/10.1681/asn.20223311s1814c","pdf_url":null,"source":{"id":"https://openalex.org/S115693375","display_name":"Journal of the American Society of Nephrology","issn_l":"1046-6673","issn":["1046-6673","1533-3450"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315620","host_organization_name":"American Society of Nephrology","host_organization_lineage":["https://openalex.org/P4310315620"],"host_organization_lineage_names":["American Society of Nephrology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Society of Nephrology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8048999905586243,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W4225420108","https://openalex.org/W4397040823","https://openalex.org/W4387529236","https://openalex.org/W2605855239","https://openalex.org/W2990206839","https://openalex.org/W2408521579","https://openalex.org/W4403398045","https://openalex.org/W1991078847","https://openalex.org/W2917872542","https://openalex.org/W2158762844"],"abstract_inverted_index":{"Background:":[0],"Juxtaglomerular":[1],"(JG)":[2],"cells":[3,13,18,48,75,80,99,116,148,163,166,340],"are":[4],"a":[5,44,70,335],"group":[6],"of":[7,24,30,46,56,73,84,94,106,113,188,202,208,224,233],"specialized":[8],"vascular":[9],"smooth":[10],"muscle":[11,87],"(VSM)":[12],"but":[14],"distinguished":[15,102],"from":[16,33,52,103],"VSM":[17,79,107,150,165],"by":[19,81,251,260,295,303,311],"the":[20,27,92,111,144,186,195,200,206,258,271,315,343],"synthesis":[21,210,345],"and":[22,117,139,149,172,176,211,229,299,305,307,346],"secretion":[23,212],"renin.":[25],"However,":[26,270,314],"intracellular":[28],"mechanisms":[29],"renin":[31,209,290,298,344],"release":[32],"JG":[34,98,115,147,162,192,339],"remain":[35],"elusive.":[36],"Methods:":[37],"We":[38,109,141,239],"performed":[39],"single-cell":[40],"RNA":[41],"seq":[42],"on":[43,91],"total":[45],"9815":[47],"that":[49,126,156,183,241,332,341],"were":[50,100,127,134,157,167,279,318],"derived":[51],"renal":[53],"cortex":[54],"tissue":[55],"C57BL/6J":[57],"mice.":[58,269],"Results:":[59],"Unbiased":[60],"clustering":[61],"analysis":[62],"demonstrated":[63],"12":[64],"distinct":[65,82],"cell":[66],"clusters.":[67],"In":[68,194,327],"particular,":[69],"cluster":[71,105],"consisting":[72],"284":[74],"was":[76],"identified":[77],"as":[78],"expression":[83,133,190,250,259,278,291],"Acta2":[85],"(\u03b1-smooth":[86],"actin).":[88],"Moreover,":[89],"based":[90],"co-expression":[93],"Ren1":[95,132,189,248,277],"(renin),":[96],"34":[97],"further":[101],"this":[104,329],"cells.":[108,151,193],"profiled":[110],"transcriptome":[112,145],"these":[114],"correlated":[118,130],"it":[119],"with":[120,131,219],"Ren1.":[121],"The":[122,152],"top":[123,153],"5":[124,154],"genes":[125,155,182],"most":[128,158],"positively":[129],"Nr2f1,":[135],"Akr1b7,":[136,170],"Smim15,":[137],"Gng11,":[138],"Sdc1.":[140,173],"also":[142],"compared":[143,262],"between":[146],"differentially":[159],"expressed":[160],"in":[161,185,191,205,213,221,230,267,276,282,292,321,338],"vs.":[164],"Ren1,":[168],"Sfrp2,":[169],"Fam46a,":[171],"Thus,":[174],"Akr1b7":[175],"Sdc1":[177,203],"could":[178],"be":[179],"potential":[180],"candidate":[181],"participate":[184],"control":[187],"present":[196],"study,":[197],"we":[198],"examined":[199],"role":[201],"(syndecan-1)":[204],"regulation":[207],"response":[214],"to":[215,263],"perfusion":[216,243,255,265,272],"pressure":[217,244,256,266,273,310],"changes":[218,275,317],"both":[220],"vitro":[222],"model":[223,232],"isolated/perfused":[225],"afferent":[226],"arteriole":[227],"(Af-Art)":[228],"vivo":[231],"2":[234],"kidneys":[235],"1":[236],"clip":[237],"(2K1C).":[238],"found":[240],"low":[242],"through":[245],"Af-Art":[246],"increased":[247,289],"mRNA":[249],"140.7\u00b124.8%":[252],"while":[253],"high":[254],"decreased":[257],"66.2\u00b113.1%":[261],"normal":[264],"WT":[268],"alteration-induced":[274],"significantly":[280,319],"attenuated":[281],"SDC1KO":[283,322],"mice":[284,323],"(n=4-5;":[285],"p<0.05).":[286],"Additionally,":[287],"2K1C":[288],"low-pressure":[293],"kidney":[294],"163.3\u00b148.7%,":[296],"plasma":[297],"Ang":[300],"II":[301],"concentrations":[302],"118.2\u00b161.4%":[304],"162.8\u00b150.2%,":[306],"mean":[308],"arterial":[309],"31.6\u00b15.7":[312],"mmHg.":[313],"2K1C-induced":[316],"reduced":[320],"(n=5-7;":[324],"p<0.01).":[325],"Conclusions:":[326],"conclusion,":[328],"study":[330],"demonstrates":[331],"syndecan-1":[333],"is":[334],"key":[336],"mechano-sensor":[337],"regulates":[342],"secretion.":[347],"Funding:":[348],"Other":[349],"NIH":[350],"Support":[351],"-":[352],"NHLBI":[353]},"counts_by_year":[],"updated_date":"2026-10-16T09:59:22.235173","created_date":"2024-05-19T00:00:00"}