{"id":"https://openalex.org/W4396990331","doi":"https://doi.org/10.1681/asn.20223311s1624b","title":"Identification of Enhancers and Their Impact on the Kidney Injury Regulator Klotho","display_name":"Identification of Enhancers and Their Impact on the Kidney Injury Regulator Klotho","publication_year":2022,"publication_date":"2022-11-01","ids":{"openalex":"https://openalex.org/W4396990331","doi":"https://doi.org/10.1681/asn.20223311s1624b"},"language":"en","primary_location":{"id":"doi:10.1681/asn.20223311s1624b","doi":"https://doi.org/10.1681/asn.20223311s1624b","is_oa":false,"landing_page_url":"https://doi.org/10.1681/asn.20223311s1624b","pdf_url":null,"source":{"id":"https://openalex.org/S115693375","display_name":"Journal of the American Society of Nephrology","issn_l":"1046-6673","issn":["1046-6673","1533-3450"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","doyens","jufo-3","ki-jl-3","medline","norway-2"],"host_organization":"https://openalex.org/P4310315620","host_organization_name":"American Society of Nephrology","host_organization_lineage":["https://openalex.org/P4310315620"],"host_organization_lineage_names":["American Society of Nephrology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Society of Nephrology","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5042715983","display_name":"Jakub Jankowski","orcid":"https://orcid.org/0000-0002-1661-079X","observed_orcids":["https://orcid.org/0000-0002-1661-079X"]},"institutions":[{"id":"https://openalex.org/I150540706","display_name":"University of Veterinary Medicine Vienna","ror":"https://ror.org/01w6qp003","country_code":"AT","type":"education","lineage":["https://openalex.org/I150540706"]},{"id":"https://openalex.org/I4210090567","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}],"countries":["AT","US"],"is_corresponding":false,"raw_author_name":"Jakub Jankowski","raw_affiliation_strings":["National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD","Veterinarmedizinische Universitat Wien, Wien, Austria"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD","institution_ids":["https://openalex.org/I4210090567"]},{"raw_affiliation_string":"Veterinarmedizinische Universitat Wien, Wien, Austria","institution_ids":["https://openalex.org/I150540706"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100615724","display_name":"Hye\u2010Kyung Lee","orcid":"https://orcid.org/0000-0002-7785-5942","observed_orcids":["https://orcid.org/0000-0002-7785-5942"]},"institutions":[{"id":"https://openalex.org/I4210090567","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hye kyung Lee","raw_affiliation_strings":["National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD","institution_ids":["https://openalex.org/I4210090567"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080158744","display_name":"Julia Wilflingseder","orcid":"https://orcid.org/0000-0002-0230-9349","observed_orcids":["https://orcid.org/0000-0002-0230-9349"]},"institutions":[{"id":"https://openalex.org/I150540706","display_name":"University of Veterinary Medicine Vienna","ror":"https://ror.org/01w6qp003","country_code":"AT","type":"education","lineage":["https://openalex.org/I150540706"]}],"countries":["AT"],"is_corresponding":false,"raw_author_name":"Julia Wilflingseder","raw_affiliation_strings":["Veterinarmedizinische Universitat Wien, Wien, Austria"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Veterinarmedizinische Universitat Wien, Wien, Austria","institution_ids":["https://openalex.org/I150540706"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5046319105","display_name":"Lothar G. Hennighausen","orcid":"https://orcid.org/0000-0001-8319-9841","observed_orcids":["https://orcid.org/0000-0001-8319-9841"]},"institutions":[{"id":"https://openalex.org/I4210090567","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210090567"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lothar Hennighausen","raw_affiliation_strings":["National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, MD","institution_ids":["https://openalex.org/I4210090567"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"33","issue":"11S","first_page":"624","last_page":"624"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10516","display_name":"Parathyroid Disorders and Treatments","score":0.5454000234603882,"subfield":{"id":"https://openalex.org/subfields/2727","display_name":"Nephrology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10516","display_name":"Parathyroid Disorders and Treatments","score":0.5454000234603882,"subfield":{"id":"https://openalex.org/subfields/2727","display_name":"Nephrology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.32910001277923584,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10684","display_name":"Acute Kidney Injury Research","score":0.013799999840557575,"subfield":{"id":"https://openalex.org/subfields/2727","display_name":"Nephrology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/klotho","display_name":"Klotho","score":0.9890000224113464},{"id":"https://openalex.org/keywords/enhancer-deletion","display_name":"enhancer deletion","score":0.6330000162124634},{"id":"https://openalex.org/keywords/kidney-injury","display_name":"kidney injury","score":0.7609999775886536},{"id":"https://openalex.org/keywords/hnf1b","display_name":"HNF1B","score":0.8080000281333923},{"id":"https://openalex.org/keywords/transcription-factor-binding-sites","display_name":"transcription factor binding sites","score":0.878000020980835},{"id":"https://openalex.org/keywords/gene-regulatory-elements","display_name":"gene regulatory elements","score":0.7689999938011169},{"id":"https://openalex.org/keywords/histone-modifications","display_name":"histone modifications","score":0.7889999747276306},{"id":"https://openalex.org/keywords/fgf23","display_name":"fibroblast growth factor 23","score":0.8650000095367432}],"concepts":[{"id":"https://openalex.org/C9832595","wikidata":"https://www.wikidata.org/wiki/Q414050","display_name":"Klotho","level":3,"score":0.8386787176132202},{"id":"https://openalex.org/C6929976","wikidata":"https://www.wikidata.org/wiki/Q3771881","display_name":"Regulator","level":3,"score":0.8093481063842773},{"id":"https://openalex.org/C111936080","wikidata":"https://www.wikidata.org/wiki/Q913367","display_name":"Enhancer","level":4,"score":0.6596884727478027},{"id":"https://openalex.org/C116834253","wikidata":"https://www.wikidata.org/wiki/Q2039217","display_name":"Identification (biology)","level":2,"score":0.6364885568618774},{"id":"https://openalex.org/C2991844318","wikidata":"https://www.wikidata.org/wiki/Q476921","display_name":"Renal injury","level":3,"score":0.5584179759025574},{"id":"https://openalex.org/C2778653478","wikidata":"https://www.wikidata.org/wiki/Q1054718","display_name":"Kidney disease","level":2,"score":0.4631476104259491},{"id":"https://openalex.org/C2780091579","wikidata":"https://www.wikidata.org/wiki/Q9377","display_name":"Kidney","level":2,"score":0.45786526799201965},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.3942570090293884},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3917819857597351},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.3647930324077606},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.1049731969833374},{"id":"https://openalex.org/C86339819","wikidata":"https://www.wikidata.org/wiki/Q407384","display_name":"Transcription factor","level":3,"score":0.08549979329109192},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0746811032295227},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1681/asn.20223311s1624b","doi":"https://doi.org/10.1681/asn.20223311s1624b","is_oa":false,"landing_page_url":"https://doi.org/10.1681/asn.20223311s1624b","pdf_url":null,"source":{"id":"https://openalex.org/S115693375","display_name":"Journal of the American Society of Nephrology","issn_l":"1046-6673","issn":["1046-6673","1533-3450"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","doyens","jufo-3","ki-jl-3","medline","norway-2"],"host_organization":"https://openalex.org/P4310315620","host_organization_name":"American Society of Nephrology","host_organization_lineage":["https://openalex.org/P4310315620"],"host_organization_lineage_names":["American Society of Nephrology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of the American Society of Nephrology","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.8371999859809875,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2886250310","https://openalex.org/W2315930086","https://openalex.org/W2810892720","https://openalex.org/W2368104841","https://openalex.org/W1545032698","https://openalex.org/W1994814634","https://openalex.org/W3127226428","https://openalex.org/W2922154562","https://openalex.org/W4385230569","https://openalex.org/W2589129978"],"abstract_inverted_index":{"Background:":[0],"Enhancer":[1,22],"activity":[2,98,221],"provides":[3,305],"new":[4,306,314],"insights":[5],"into":[6,115,308],"the":[7,71,116,127,132,135,161,187,212,249,318,324,343],"gene":[8,72,122,128,184,320],"expression":[9,185,258,294,335],"machinery":[10],"and":[11,20,46,56,79,142,180,189,194,215,222,281,351],"is":[12,24,49,62,339],"emerging":[13],"as":[14,36,275,321,323],"a":[15,37,50,63,100,109,190,313],"valuable":[16],"target":[17],"of":[18,28,66,70,94,112,118,134,145,171,227,248,256,295,317,342],"research":[19],"therapy.":[21],"disruption":[23],"involved":[25],"in":[26,60,73,76,99,160,186,206,234,260],"development":[27],"human":[29],"disease,":[30,68],"but":[31,229,239],"can":[32],"also":[33,200],"be":[34,349],"used":[35,108,150,178,201],"tool":[38],"to":[39,130,152,182,196,203,218,288,345,348],"investigate":[40,131,183,197],"genes":[41,297],"key":[42],"for":[43,328],"disease":[44],"progression":[45],"recovery.":[47],"There":[48],"well-known":[51],"connection":[52],"between":[53],"kidney":[54,67,101,300],"function":[55],"Klotho.":[57,119],"While":[58],"decrease":[59,259],"Klotho":[61,85,140,166,213,237,266,272,309,319,334],"known":[64],"sign":[65],"deletion":[69,170],"mice":[74,268,344],"results":[75],"severe":[77],"phenotype":[78,338],"greatly":[80],"shortened":[81],"lifespan,":[82,276],"making":[83],"investigating":[84],"knockouts":[86],"challenging.":[87],"Previously,":[88],"we":[89,107],"identified":[90,312],"two":[91,136],"putative":[92,137],"enhancers":[93,138],"Klotho,":[95],"which":[96],"lost":[97],"injury":[102,346],"setting.":[103],"In":[104],"this":[105],"study,":[106],"novel":[110],"way":[111],"gaining":[113],"insight":[114,307],"role":[117],"We":[120,149,177,199,311],"alteried":[121],"regulatory":[123,315],"elements,":[124],"rather":[125],"than":[126],"itself,":[129],"impact":[133,356],"on":[139,211,357],"expression,":[141,238],"physiological":[143],"effects":[144],"their":[146,220],"deletion.":[147],"Methods:":[148],"Crispr/Cas9":[151],"generate":[153],"six":[154],"viable":[155],"mouse":[156],"lines":[157],"with":[158,209,299],"deletions":[159],"distal,":[162,228],"proximal":[163,231,250],"or":[164],"both":[165],"enhancers,":[167],"including":[168],"selective":[169],"HNF1b":[172,242],"transcription":[173,325],"factor":[174,326],"binding":[175,243],"site.":[176],"qPCR":[179],"RNA-seq":[181,290],"kidney,":[188],"blood":[191],"biochemistry":[192],"panel":[193],"ELISA":[195],"phenotype.":[198],"Chip-seq":[202],"analyze":[204],"changes":[205],"histone":[207],"modifications":[208],"focus":[210],"promoter":[214],"enhancer":[216,232,251],"regions":[217],"assess":[219],"verify":[223],"deletions.":[224],"Results:":[225],"Deletion":[226],"not":[230,270,285],"resulted":[233],"50%":[235],"lower":[236,333],"only":[240],"when":[241],"site":[244],"was":[245,252],"impacted.":[246],"Inactivity":[247],"confirmed":[253],"by":[254],"lack":[255],"further":[257,353],"double":[261],"knockout":[262,273],"mice.":[263],"Despite":[264,331],"significant":[265],"depletion,":[267],"did":[269],"display":[271],"phenotype,":[274],"weight,":[277],"serum":[278],"phosphate,":[279],"calcium":[280],"FGF23":[282],"levels":[283],"were":[284],"different":[286],"compared":[287],"controls.":[289],"analysis":[291],"revealed":[292],"altered":[293],"several":[296],"associated":[298],"disease.":[301],"Conclusions:":[302],"Our":[303],"study":[304],"regulation.":[310],"element":[316],"well":[322],"responsible":[327],"its":[329],"activity.":[330],"significantly":[332],"levels,":[336],"normal":[337],"preserved.":[340],"Susceptibility":[341],"remains":[347],"investigated":[350],"will":[352],"elucidate":[354],"Klotho's":[355],"renal":[358],"health.":[359],"Funding:":[360],"NIDDK":[361],"Support":[362]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2025-10-10T00:00:00"}