{"id":"https://openalex.org/W4396931473","doi":"https://doi.org/10.3390/genes15050624","title":"The Effect of Short- and Long-Term Cryopreservation on Chicken Primordial Germ Cells","display_name":"The Effect of Short- and Long-Term Cryopreservation on Chicken Primordial Germ Cells","publication_year":2024,"publication_date":"2024-05-14","ids":{"openalex":"https://openalex.org/W4396931473","doi":"https://doi.org/10.3390/genes15050624","pmid":"https://pubmed.ncbi.nlm.nih.gov/38790253","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/11121574"},"language":"en","primary_location":{"id":"doi:10.3390/genes15050624","doi":"https://doi.org/10.3390/genes15050624","is_oa":true,"landing_page_url":"https://doi.org/10.3390/genes15050624","pdf_url":null,"source":{"id":"https://openalex.org/S194631498","display_name":"Genes","issn_l":"2073-4425","issn":["2073-4425"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genes","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.3390/genes15050624","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5055712037","display_name":"Mariam Ibrahim","orcid":"https://orcid.org/0000-0001-6471-0237","observed_orcids":["https://orcid.org/0000-0001-6471-0237"]},"institutions":[{"id":"https://openalex.org/I1300393620","display_name":"Bydgoszcz University of Science and Technology","ror":"https://ror.org/049eq0c58","country_code":"PL","type":"education","lineage":["https://openalex.org/I1300393620"]}],"countries":["PL"],"is_corresponding":true,"raw_author_name":"Mariam Ibrahim","raw_affiliation_strings":["Department of Animal Biotechnology and Genetics, Bydgoszcz University of Science and Technology, Mazowiecka 28, 85-084 Bydgoszcz, Poland","PBS Doctoral School, Bydgoszcz University of Science and Technology, Aleje Prof. S. Kaliskiego 7, 85-796 Bydgoszcz, Poland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Animal Biotechnology and Genetics, Bydgoszcz University of Science and Technology, Mazowiecka 28, 85-084 Bydgoszcz, Poland","institution_ids":["https://openalex.org/I1300393620"]},{"raw_affiliation_string":"PBS Doctoral School, Bydgoszcz University of Science and Technology, Aleje Prof. S. Kaliskiego 7, 85-796 Bydgoszcz, Poland","institution_ids":["https://openalex.org/I1300393620"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101935500","display_name":"Ewa Grochowska","orcid":"https://orcid.org/0000-0001-5554-023X","observed_orcids":["https://orcid.org/0000-0001-5554-023X"]},"institutions":[{"id":"https://openalex.org/I1300393620","display_name":"Bydgoszcz University of Science and Technology","ror":"https://ror.org/049eq0c58","country_code":"PL","type":"education","lineage":["https://openalex.org/I1300393620"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Ewa Grochowska","raw_affiliation_strings":["Department of Animal Biotechnology and Genetics, Bydgoszcz University of Science and Technology, Mazowiecka 28, 85-084 Bydgoszcz, Poland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Animal Biotechnology and Genetics, Bydgoszcz University of Science and Technology, Mazowiecka 28, 85-084 Bydgoszcz, Poland","institution_ids":["https://openalex.org/I1300393620"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066313585","display_name":"Bence L\u00e1z\u00e1r","orcid":"https://orcid.org/0000-0002-0716-6113","observed_orcids":["https://orcid.org/0000-0002-0716-6113"]},"institutions":[{"id":"https://openalex.org/I1290523313","display_name":"Magyar Agr\u00e1r- \u00e9s \u00c9lettudom\u00e1nyi Egyetem","ror":"https://ror.org/01394d192","country_code":"HU","type":"education","lineage":["https://openalex.org/I1290523313"]},{"id":"https://openalex.org/I4405253082","display_name":"National Centre for Biodiversity and Gene Conservation","ror":"https://ror.org/009rdzd88","country_code":"HU","type":"government","lineage":["https://openalex.org/I4405253082"]}],"countries":["HU"],"is_corresponding":false,"raw_author_name":"Bence L\u00e1z\u00e1r","raw_affiliation_strings":["Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, Szent-Gyorgyi Albert Street 4, 2100 Godollo, Hungary","National Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, Isaszegi Street 200, 2100 Godollo, Hungary"],"raw_orcid":"https://orcid.org/0000-0002-0716-6113","affiliations":[{"raw_affiliation_string":"Institute of Genetics and Biotechnology, Hungarian University of Agriculture and Life Sciences, Szent-Gyorgyi Albert Street 4, 2100 Godollo, Hungary","institution_ids":["https://openalex.org/I1290523313"]},{"raw_affiliation_string":"National Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, Isaszegi Street 200, 2100 Godollo, Hungary","institution_ids":["https://openalex.org/I4405253082"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001349780","display_name":"Eszter Patakin\u00e9 V\u00e1rkonyi","orcid":"https://orcid.org/0000-0003-0114-5255","observed_orcids":["https://orcid.org/0000-0003-0114-5255"]},"institutions":[{"id":"https://openalex.org/I4405253082","display_name":"National Centre for Biodiversity and Gene Conservation","ror":"https://ror.org/009rdzd88","country_code":"HU","type":"government","lineage":["https://openalex.org/I4405253082"]}],"countries":["HU"],"is_corresponding":false,"raw_author_name":"Eszter V\u00e1rkonyi","raw_affiliation_strings":["National Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, Isaszegi Street 200, 2100 Godollo, Hungary"],"raw_orcid":"https://orcid.org/0000-0003-0114-5255","affiliations":[{"raw_affiliation_string":"National Centre for Biodiversity and Gene Conservation, Institute for Farm Animal Gene Conservation, Isaszegi Street 200, 2100 Godollo, Hungary","institution_ids":["https://openalex.org/I4405253082"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005669901","display_name":"Marek F. Bednarczyk","orcid":"https://orcid.org/0000-0003-4515-193X","observed_orcids":["https://orcid.org/0000-0003-4515-193X"]},"institutions":[{"id":"https://openalex.org/I1300393620","display_name":"Bydgoszcz University of Science and Technology","ror":"https://ror.org/049eq0c58","country_code":"PL","type":"education","lineage":["https://openalex.org/I1300393620"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Marek Bednarczyk","raw_affiliation_strings":["Department of Animal Biotechnology and Genetics, Bydgoszcz University of Science and Technology, Mazowiecka 28, 85-084 Bydgoszcz, Poland"],"raw_orcid":"https://orcid.org/0000-0003-4515-193X","affiliations":[{"raw_affiliation_string":"Department of Animal Biotechnology and Genetics, Bydgoszcz University of Science and Technology, Mazowiecka 28, 85-084 Bydgoszcz, Poland","institution_ids":["https://openalex.org/I1300393620"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5003240323","display_name":"Katarzyna Stadnicka","orcid":"https://orcid.org/0000-0002-2349-7203","observed_orcids":["https://orcid.org/0000-0002-2349-7203"]},"institutions":[{"id":"https://openalex.org/I3019271933","display_name":"Nicolaus Copernicus University","ror":"https://ror.org/0102mm775","country_code":"PL","type":"education","lineage":["https://openalex.org/I3019271933"]},{"id":"https://openalex.org/I4210148386","display_name":"Collegium Medicum in Bydgoszcz","ror":"https://ror.org/04c5jwj47","country_code":"PL","type":"education","lineage":["https://openalex.org/I3019271933","https://openalex.org/I4210148386"]}],"countries":["PL"],"is_corresponding":false,"raw_author_name":"Katarzyna Stadnicka","raw_affiliation_strings":["Faculty of Health Sciences, Collegium Medicum, Nicolaus Copernicus University, \u0141ukasiewicza 1, 85-821 Bydgoszcz, Poland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Health Sciences, Collegium Medicum, Nicolaus Copernicus University, \u0141ukasiewicza 1, 85-821 Bydgoszcz, Poland","institution_ids":["https://openalex.org/I3019271933","https://openalex.org/I4210148386"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":5,"corresponding_author_ids":["https://openalex.org/A5055712037"],"corresponding_institution_ids":["https://openalex.org/I1300393620"],"apc_list":{"value":2600,"currency":"CHF","value_usd":2955},"apc_paid":{"value":2600,"currency":"CHF","value_usd":2955},"fwci":2.7519,"has_fulltext":true,"cited_by_count":14,"citation_normalized_percentile":{"value":0.91053776,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"15","issue":"5","first_page":"624","last_page":"624"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.9398999810218811,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.9398999810218811,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.0210999995470047,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10116","display_name":"Sperm and Testicular Function","score":0.019300000742077827,"subfield":{"id":"https://openalex.org/subfields/2743","display_name":"Reproductive Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/primordial-germ-cells","display_name":"primordial germ cells","score":0.9589999914169312},{"id":"https://openalex.org/keywords/chickens","display_name":"chickens","score":0.8619999885559082},{"id":"https://openalex.org/keywords/cryopreservation","display_name":"cryopreservation","score":0.8500000238418579},{"id":"https://openalex.org/keywords/germ-cell-markers","display_name":"germ cell markers","score":0.8149999976158142},{"id":"https://openalex.org/keywords/cd15","display_name":"CD15","score":0.8270000219345093},{"id":"https://openalex.org/keywords/dazl","display_name":"DAZL","score":1.0},{"id":"https://openalex.org/keywords/oct4","display_name":"Oct4","score":0.8980000019073486},{"id":"https://openalex.org/keywords/nanog","display_name":"Nanog","score":1.0},{"id":"https://openalex.org/keywords/pluripotency","display_name":"pluripotency","score":0.722000002861023},{"id":"https://openalex.org/keywords/in-vitro-culture","display_name":"in vitro culture","score":0.7080000042915344},{"id":"https://openalex.org/keywords/germ-cell-maintenance","display_name":"germ cell maintenance","score":0.3529999852180481},{"id":"https://openalex.org/keywords/genetic-conservation","display_name":"genetic conservation","score":0.5550000071525574},{"id":"https://openalex.org/keywords/epigenetic-inheritance","display_name":"epigenetic inheritance","score":0.7200000286102295}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8656312823295593},{"id":"https://openalex.org/C179933525","wikidata":"https://www.wikidata.org/wiki/Q1144664","display_name":"Cryopreservation","level":3,"score":0.7987493872642517},{"id":"https://openalex.org/C207701140","wikidata":"https://www.wikidata.org/wiki/Q420522","display_name":"Homeobox protein NANOG","level":5,"score":0.6948143243789673},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.6626928448677063},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.5578335523605347},{"id":"https://openalex.org/C41091548","wikidata":"https://www.wikidata.org/wiki/Q26939","display_name":"Epigenetics","level":3,"score":0.5554295778274536},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.5230530500411987},{"id":"https://openalex.org/C2779122487","wikidata":"https://www.wikidata.org/wiki/Q281579","display_name":"Germ cell","level":3,"score":0.4829968214035034},{"id":"https://openalex.org/C112190416","wikidata":"https://www.wikidata.org/wiki/Q2124450","display_name":"RNA extraction","level":4,"score":0.4807741045951843},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4346328377723694},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.41764092445373535},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.3997018337249756},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3671504259109497},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.32195696234703064},{"id":"https://openalex.org/C107459253","wikidata":"https://www.wikidata.org/wiki/Q674925","display_name":"Induced pluripotent stem cell","level":4,"score":0.09642475843429565},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002642","descriptor_name":"Chick Embryo","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002645","descriptor_name":"Chickens","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005854","descriptor_name":"Germ Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D005854","descriptor_name":"Germ Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015415","descriptor_name":"Biomarkers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015925","descriptor_name":"Cryopreservation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.3390/genes15050624","doi":"https://doi.org/10.3390/genes15050624","is_oa":true,"landing_page_url":"https://doi.org/10.3390/genes15050624","pdf_url":null,"source":{"id":"https://openalex.org/S194631498","display_name":"Genes","issn_l":"2073-4425","issn":["2073-4425"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genes","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:11121574","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11121574","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11121574/pdf/genes-15-00624.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genes (Basel)","raw_type":"Text"},{"id":"pmid:38790253","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/38790253","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genes","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.3390/genes15050624","doi":"https://doi.org/10.3390/genes15050624","is_oa":true,"landing_page_url":"https://doi.org/10.3390/genes15050624","pdf_url":null,"source":{"id":"https://openalex.org/S194631498","display_name":"Genes","issn_l":"2073-4425","issn":["2073-4425"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Genes","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1138680913","display_name":null,"funder_award_id":"UMO-2017/27/B/NZ9/01510","funder_id":"https://openalex.org/F4320322650","funder_display_name":"Narodowym Centrum Nauki"},{"id":"https://openalex.org/G2382874029","display_name":null,"funder_award_id":"UMO-2020/37/B/NZ9/00497","funder_id":"https://openalex.org/F4320322650","funder_display_name":"Narodowym Centrum Nauki"},{"id":"https://openalex.org/G2570643425","display_name":null,"funder_award_id":"2020/37/B/NZ9/00497","funder_id":"https://openalex.org/F4320322650","funder_display_name":"Narodowym Centrum Nauki"}],"funders":[{"id":"https://openalex.org/F4320322650","display_name":"Narodowym Centrum Nauki","ror":"https://ror.org/03ha2q922"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4396931473.pdf","grobid_xml":"https://content.openalex.org/works/W4396931473.grobid-xml"},"referenced_works_count":52,"referenced_works":["https://openalex.org/W1847746664","https://openalex.org/W1912418335","https://openalex.org/W1965972575","https://openalex.org/W1981323595","https://openalex.org/W1992303587","https://openalex.org/W1995032020","https://openalex.org/W2004914865","https://openalex.org/W2005064969","https://openalex.org/W2010093125","https://openalex.org/W2014282876","https://openalex.org/W2015242108","https://openalex.org/W2020529579","https://openalex.org/W2021235728","https://openalex.org/W2021290198","https://openalex.org/W2032564299","https://openalex.org/W2042815855","https://openalex.org/W2055547316","https://openalex.org/W2061921583","https://openalex.org/W2071282863","https://openalex.org/W2100303227","https://openalex.org/W2107216847","https://openalex.org/W2117479891","https://openalex.org/W2126851387","https://openalex.org/W2137136678","https://openalex.org/W2161286469","https://openalex.org/W2173835926","https://openalex.org/W2339165406","https://openalex.org/W2399463045","https://openalex.org/W2406823956","https://openalex.org/W2560186525","https://openalex.org/W2748855237","https://openalex.org/W2762822370","https://openalex.org/W2796543623","https://openalex.org/W2802777998","https://openalex.org/W2901129118","https://openalex.org/W2921406196","https://openalex.org/W2978346470","https://openalex.org/W3087654154","https://openalex.org/W3109045550","https://openalex.org/W3135240616","https://openalex.org/W3157302156","https://openalex.org/W3162602453","https://openalex.org/W3174203494","https://openalex.org/W4223537584","https://openalex.org/W4235676496","https://openalex.org/W4280503455","https://openalex.org/W4322577006","https://openalex.org/W4322741244","https://openalex.org/W4327972269","https://openalex.org/W4365515292","https://openalex.org/W4382004338","https://openalex.org/W4389280675"],"related_works":["https://openalex.org/W2270972729","https://openalex.org/W2255496040","https://openalex.org/W7204461120","https://openalex.org/W2389268422","https://openalex.org/W1992303587","https://openalex.org/W2139451186","https://openalex.org/W2233489422","https://openalex.org/W2388477882","https://openalex.org/W4403158067","https://openalex.org/W2035766175"],"abstract_inverted_index":{"Primordial":[0],"germ":[1,185],"cells":[2,27],"(PGCs)":[3],"are":[4],"the":[5,11,70,75,86,128,180,222],"precursors":[6],"of":[7,16,77,88,156,183],"functional":[8],"gametes":[9],"and":[10,19,36,58,81,119,146,190,198,219],"only":[12],"cell":[13,186],"type":[14],"capable":[15],"transmitting":[17],"genetic":[18,34,37],"epigenetic":[20,42],"information":[21],"from":[22,99],"generation":[23],"to":[24,73,127,178,233],"generation.":[25],"These":[26],"offer":[28],"valuable":[29],"starting":[30],"material":[31],"for":[32,117,140],"cell-based":[33],"engineering":[35],"preservation,":[38],"as":[39,41,133,192,194],"well":[40,193],"studies.":[43],"While":[44],"chicken":[45,103],"PGCs":[46,96,131,158,207,230],"have":[47],"demonstrated":[48,225],"resilience":[49],"in":[50,82,148,229,236],"maintaining":[51],"their":[52,60,237],"germness":[53],"characteristics":[54],"during":[55],"both":[56],"culturing":[57],"cryopreservation,":[59],"handling":[61],"remains":[62],"a":[63,141],"complex":[64],"challenge":[65],"requiring":[66],"further":[67],"refinement.":[68],"Herein,":[69],"study":[71],"aimed":[72],"compare":[74],"effects":[76],"different":[78],"conditions":[79,125],"(freezing-thawing":[80],"vitro":[83,149],"cultivation)":[84],"on":[85],"expression":[87,181,205,227],"PGC-specific":[89],"marker":[90],"genes.":[91],"Embryonic":[92],"blood":[93,113,129],"containing":[94,130],"circulating":[95],"was":[97,114,166],"isolated":[98,239],"purebred":[100],"Green-legged":[101],"Partridgelike":[102],"embryos":[104],"at":[105],"14\u201316":[106],"Hamburger\u2013Hamilton":[107],"(HH)":[108],"embryonic":[109],"development":[110],"stage.":[111],"The":[112,124,200],"pooled":[115],"separately":[116],"males":[118,218],"females":[120],"following":[121],"sex":[122],"determination.":[123],"applied":[126],"were":[132],"follows:":[134],"(1)":[135],"fresh":[136],"isolation;":[137],"(2)":[138],"cryopreservation":[139,155],"short":[142],"term":[143],"(2":[144],"days);":[145],"(3)":[147],"culture":[150],"(3":[151],"months)":[152],"with":[153,212],"long-term":[154],"purified":[157],"(~2":[159],"years).":[160],"To":[161],"characterize":[162],"PGCs,":[163],"RNA":[164],"isolation":[165],"carried":[167],"out,":[168],"followed":[169],"by":[170],"quantitative":[171],"reverse":[172],"transcription":[173],"polymerase":[174],"chain":[175],"reaction":[176],"(RT-qPCR)":[177],"assess":[179],"levels":[182,228],"specific":[184],"markers":[187,196,224],"(SSEA1,":[188],"CVH,":[189],"DAZL),":[191],"pluripotency":[195],"(OCT4":[197],"NANOG).":[199],"investigated":[201],"genes":[202],"exhibited":[203],"consistent":[204],"among":[206],"maintained":[208],"under":[209],"diverse":[210],"conditions,":[211],"no":[213],"discernible":[214],"differences":[215],"observed":[216],"between":[217],"females.":[220],"Notably,":[221],"analyzed":[223],"higher":[226],"when":[231],"subjected":[232],"freezing":[234],"than":[235],"freshly":[238],"counterparts.":[240]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":8},{"year":2024,"cited_by_count":1}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2024-05-16T00:00:00"}