{"id":"https://openalex.org/W4396642201","doi":"https://doi.org/10.1002/cyto.a.24840","title":"Simple, fast, cost\u2010efficient, reliable, and highly automated DNA content analysis of cells in adherent cultures","display_name":"Simple, fast, cost\u2010efficient, reliable, and highly automated DNA content analysis of cells in adherent cultures","publication_year":2024,"publication_date":"2024-05-03","ids":{"openalex":"https://openalex.org/W4396642201","doi":"https://doi.org/10.1002/cyto.a.24840","pmid":"https://pubmed.ncbi.nlm.nih.gov/38702936"},"language":"en","primary_location":{"id":"doi:10.1002/cyto.a.24840","doi":"https://doi.org/10.1002/cyto.a.24840","is_oa":true,"landing_page_url":"https://doi.org/10.1002/cyto.a.24840","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/cyto.a.24840","source":{"id":"https://openalex.org/S86468533","display_name":"Cytometry Part A","issn_l":"1552-4922","issn":["1552-4922","1552-4930"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cytometry Part A","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/cyto.a.24840","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5005538169","display_name":"Jana \u010c\u00ed\u017ekov\u00e1","orcid":"https://orcid.org/0000-0003-3204-3091","observed_orcids":["https://orcid.org/0000-0003-3204-3091"]},"institutions":[{"id":"https://openalex.org/I96415178","display_name":"University of Defence","ror":"https://ror.org/04arkmn57","country_code":"CZ","type":"education","lineage":["https://openalex.org/I96415178"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Jana \u010c\u00ed\u017ekov\u00e1","raw_affiliation_strings":["Department of Radiobiology, Military Faculty of Medicine, University of Defence, Hradec Kralove, Czech Republic"],"raw_orcid":"https://orcid.org/0000-0003-3204-3091","affiliations":[{"raw_affiliation_string":"Department of Radiobiology, Military Faculty of Medicine, University of Defence, Hradec Kralove, Czech Republic","institution_ids":["https://openalex.org/I96415178"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073753102","display_name":"Al\u017eb\u011bta Filipov\u00e1","orcid":"https://orcid.org/0000-0002-3849-6449","observed_orcids":["https://orcid.org/0000-0002-3849-6449"]},"institutions":[{"id":"https://openalex.org/I96415178","display_name":"University of Defence","ror":"https://ror.org/04arkmn57","country_code":"CZ","type":"education","lineage":["https://openalex.org/I96415178"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Al\u017eb\u011bta Filipov\u00e1","raw_affiliation_strings":["Department of Radiobiology, Military Faculty of Medicine, University of Defence, Hradec Kralove, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Radiobiology, Military Faculty of Medicine, University of Defence, Hradec Kralove, Czech Republic","institution_ids":["https://openalex.org/I96415178"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087734367","display_name":"Anna Lierov\u00e1","orcid":"https://orcid.org/0000-0003-0620-9588","observed_orcids":["https://orcid.org/0000-0003-0620-9588"]},"institutions":[{"id":"https://openalex.org/I96415178","display_name":"University of Defence","ror":"https://ror.org/04arkmn57","country_code":"CZ","type":"education","lineage":["https://openalex.org/I96415178"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Anna Carrillo","raw_affiliation_strings":["Department of Radiobiology, Military Faculty of Medicine, University of Defence, Hradec Kralove, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Radiobiology, Military Faculty of Medicine, University of Defence, Hradec Kralove, Czech Republic","institution_ids":["https://openalex.org/I96415178"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5034078040","display_name":"Marie Ehrlichov\u00e1","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210136939","display_name":"National Institute of Public Health","ror":"https://ror.org/04ftj7e51","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I4210098783","https://openalex.org/I4210136939"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Marie Ehrlichov\u00e1","raw_affiliation_strings":["Toxicogenomics Unit, Centre of Toxicology and Health Safety, National Institute of Public Health, Prague, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Toxicogenomics Unit, Centre of Toxicology and Health Safety, National Institute of Public Health, Prague, Czech Republic","institution_ids":["https://openalex.org/I4210136939"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053574452","display_name":"Al\u017eb\u011bta Sp\u00e1lenkov\u00e1","orcid":"https://orcid.org/0000-0002-4704-0283","observed_orcids":["https://orcid.org/0000-0002-4704-0283"]},"institutions":[{"id":"https://openalex.org/I4210136939","display_name":"National Institute of Public Health","ror":"https://ror.org/04ftj7e51","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I4210098783","https://openalex.org/I4210136939"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Al\u017eb\u011bta Sp\u00e1lenkov\u00e1","raw_affiliation_strings":["Toxicogenomics Unit, Centre of Toxicology and Health Safety, National Institute of Public Health, Prague, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Toxicogenomics Unit, Centre of Toxicology and Health Safety, National Institute of Public Health, Prague, Czech Republic","institution_ids":["https://openalex.org/I4210136939"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079667154","display_name":"Al\u017eb\u011bta Magdolenov\u00e1","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I202391551","display_name":"Czech Academy of Sciences","ror":"https://ror.org/053avzc18","country_code":"CZ","type":"government","lineage":["https://openalex.org/I202391551"]},{"id":"https://openalex.org/I4210145889","display_name":"Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry","ror":"https://ror.org/04nfjn472","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I202391551","https://openalex.org/I4210145889"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Al\u017ebeta Magdolenov\u00e1","raw_affiliation_strings":["Biochemical Pharmacology, Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biochemical Pharmacology, Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic","institution_ids":["https://openalex.org/I202391551","https://openalex.org/I4210145889"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5055867874","display_name":"Miroslav H\u00e1jek","orcid":"https://orcid.org/0000-0002-8906-243X","observed_orcids":["https://orcid.org/0000-0002-8906-243X"]},"institutions":[{"id":"https://openalex.org/I202391551","display_name":"Czech Academy of Sciences","ror":"https://ror.org/053avzc18","country_code":"CZ","type":"government","lineage":["https://openalex.org/I202391551"]},{"id":"https://openalex.org/I4210145889","display_name":"Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry","ror":"https://ror.org/04nfjn472","country_code":"CZ","type":"facility","lineage":["https://openalex.org/I202391551","https://openalex.org/I4210145889"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Miroslav H\u00e1jek","raw_affiliation_strings":["Biochemical Pharmacology, Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biochemical Pharmacology, Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic","institution_ids":["https://openalex.org/I202391551","https://openalex.org/I4210145889"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101479177","display_name":"Pavel Hor\u00e1k","orcid":"https://orcid.org/0000-0002-4638-1529","observed_orcids":["https://orcid.org/0000-0002-4638-1529"]},"institutions":[{"id":"https://openalex.org/I21250087","display_name":"Charles University","ror":"https://ror.org/024d6js02","country_code":"CZ","type":"education","lineage":["https://openalex.org/I21250087"]},{"id":"https://openalex.org/I2803076161","display_name":"Fakultn\u00ed nemocnice Bulovka","ror":"https://ror.org/009e9xr64","country_code":"CZ","type":"healthcare","lineage":["https://openalex.org/I2803076161"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Pavel Hor\u00e1k","raw_affiliation_strings":["Surgical Department, 1st Medical Faculty of Charles University and Faculty Hospital Bulovka, Prague, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Surgical Department, 1st Medical Faculty of Charles University and Faculty Hospital Bulovka, Prague, Czech Republic","institution_ids":["https://openalex.org/I21250087","https://openalex.org/I2803076161"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5096199094","display_name":"Aneta Erbenova","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I21250087","display_name":"Charles University","ror":"https://ror.org/024d6js02","country_code":"CZ","type":"education","lineage":["https://openalex.org/I21250087"]},{"id":"https://openalex.org/I2803076161","display_name":"Fakultn\u00ed nemocnice Bulovka","ror":"https://ror.org/009e9xr64","country_code":"CZ","type":"healthcare","lineage":["https://openalex.org/I2803076161"]}],"countries":["CZ"],"is_corresponding":false,"raw_author_name":"Aneta Erbenova","raw_affiliation_strings":["Surgical Department, 1st Medical Faculty of Charles University and Faculty Hospital Bulovka, Prague, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Surgical Department, 1st Medical Faculty of Charles University and Faculty Hospital Bulovka, Prague, Czech Republic","institution_ids":["https://openalex.org/I21250087","https://openalex.org/I2803076161"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5013420163","display_name":"Zuzana \u0160inkorov\u00e1","orcid":"https://orcid.org/0000-0003-0396-7447","observed_orcids":["https://orcid.org/0000-0003-0396-7447"]},"institutions":[{"id":"https://openalex.org/I96415178","display_name":"University of Defence","ror":"https://ror.org/04arkmn57","country_code":"CZ","type":"education","lineage":["https://openalex.org/I96415178"]}],"countries":["CZ"],"is_corresponding":true,"raw_author_name":"Zuzana \u0160inkorov\u00e1","raw_affiliation_strings":["Department of Radiobiology, Military Faculty of Medicine, University of Defence, Hradec Kralove, Czech Republic"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Radiobiology, Military Faculty of Medicine, University of Defence, Hradec Kralove, Czech Republic","institution_ids":["https://openalex.org/I96415178"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":6,"corresponding_author_ids":["https://openalex.org/A5013420163"],"corresponding_institution_ids":["https://openalex.org/I96415178"],"apc_list":{"value":3780,"currency":"USD","value_usd":3780},"apc_paid":null,"fwci":0.3284,"has_fulltext":true,"cited_by_count":2,"citation_normalized_percentile":{"value":0.44782448,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"105","issue":"6","first_page":"474","last_page":"479"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.7588000297546387,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.7588000297546387,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.02329999953508377,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10433","display_name":"Carcinogens and Genotoxicity Assessment","score":0.02280000038444996,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/dna-content-analysis","display_name":"DNA content analysis","score":0.9779999852180481},{"id":"https://openalex.org/keywords/adherent-cell-culture","display_name":"adherent cell culture","score":0.9010000228881836},{"id":"https://openalex.org/keywords/flow-cytometry","display_name":"flow cytometry","score":0.8640000224113464},{"id":"https://openalex.org/keywords/propidium-iodide-staining","display_name":"propidium iodide staining","score":0.7979999780654907},{"id":"https://openalex.org/keywords/sample-preparation-simplification","display_name":"sample preparation simplification","score":0.5410000085830688},{"id":"https://openalex.org/keywords/high-throughput-assay","display_name":"high-throughput assay","score":0.7160000205039978},{"id":"https://openalex.org/keywords/cell-cycle-analysis","display_name":"cell cycle analysis","score":0.7139999866485596}],"concepts":[{"id":"https://openalex.org/C2775934118","wikidata":"https://www.wikidata.org/wiki/Q424169","display_name":"Propidium iodide","level":4,"score":0.6295510530471802},{"id":"https://openalex.org/C57409179","wikidata":"https://www.wikidata.org/wiki/Q1218293","display_name":"Lysis","level":2,"score":0.5194467902183533},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5160300731658936},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.466006338596344},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.37554746866226196},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3731289505958557},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3495730757713318},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.34513071179389954},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.17797669768333435},{"id":"https://openalex.org/C31573885","wikidata":"https://www.wikidata.org/wiki/Q304484","display_name":"Programmed cell death","level":3,"score":0.0},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000782","descriptor_name":"Aneuploidy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001331","descriptor_name":"Automation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002448","descriptor_name":"Cell Adhesion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002453","descriptor_name":"Cell Cycle","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015203","descriptor_name":"Reproducibility of Results","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/cyto.a.24840","doi":"https://doi.org/10.1002/cyto.a.24840","is_oa":true,"landing_page_url":"https://doi.org/10.1002/cyto.a.24840","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/cyto.a.24840","source":{"id":"https://openalex.org/S86468533","display_name":"Cytometry Part A","issn_l":"1552-4922","issn":["1552-4922","1552-4930"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cytometry Part A","raw_type":"journal-article"},{"id":"pmid:38702936","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/38702936","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cytometry. Part A : the journal of the International Society for Analytical Cytology","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1002/cyto.a.24840","doi":"https://doi.org/10.1002/cyto.a.24840","is_oa":true,"landing_page_url":"https://doi.org/10.1002/cyto.a.24840","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/cyto.a.24840","source":{"id":"https://openalex.org/S86468533","display_name":"Cytometry Part A","issn_l":"1552-4922","issn":["1552-4922","1552-4930"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cytometry Part A","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G3822909593","display_name":"The role of Notch signaling pathway in mechanism of action of novel taxanes derivatives","funder_award_id":"GA21-14082S","funder_id":"https://openalex.org/F4320321006","funder_display_name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky"}],"funders":[{"id":"https://openalex.org/F4320321005","display_name":"Ministerstvo \u0160kolstv\u00ed, Ml\u00e1de\u017ee a T\u011blov\u00fdchovy","ror":"https://ror.org/037n8p820"},{"id":"https://openalex.org/F4320321006","display_name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","ror":"https://ror.org/01pv73b02"},{"id":"https://openalex.org/F4320321798","display_name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","ror":"https://ror.org/00y6khe77"},{"id":"https://openalex.org/F4320328734","display_name":"Ministerstvo Obrany \u010cesk\u00e9 Republiky","ror":"https://ror.org/00r7raa16"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4396642201.pdf","grobid_xml":"https://content.openalex.org/works/W4396642201.grobid-xml"},"referenced_works_count":8,"referenced_works":["https://openalex.org/W1767073733","https://openalex.org/W1989063000","https://openalex.org/W2019145355","https://openalex.org/W2032319737","https://openalex.org/W2056793762","https://openalex.org/W2083072213","https://openalex.org/W2782729392","https://openalex.org/W4235958526"],"related_works":["https://openalex.org/W2150341677","https://openalex.org/W2405009236","https://openalex.org/W2740319795","https://openalex.org/W4402205047","https://openalex.org/W2616287426","https://openalex.org/W4327665309","https://openalex.org/W151714794","https://openalex.org/W4254045749","https://openalex.org/W2887619755","https://openalex.org/W1967097378"],"abstract_inverted_index":{"The":[0],"most":[1],"commonly":[2],"used":[3],"flow":[4],"cytometric":[5],"(FCM)":[6],"analysis":[7,136],"of":[8,57,66,91,117],"cellular":[9],"DNA":[10,67,129,155],"content":[11],"relies":[12],"on":[13],"ethanol":[14,78],"fixation":[15,79],"followed":[16],"by":[17,73],"RNA":[18],"digestion":[19],"and":[20,32,44,83,137,142,151],"propidium":[21],"iodide":[22],"(PI)":[23],"intercalation":[24],"into":[25],"double-stranded":[26],"DNA.":[27],"This":[28],"is":[29,36],"a":[30,63,102,111,122,147],"laborious":[31],"time-consuming":[33],"procedure":[34],"that":[35,75],"subject":[37],"to":[38,42],"systematic":[39],"errors":[40],"due":[41],"centrifugation":[43],"washing":[45,82],"steps":[46],"associated":[47],"with":[48,121],"sample":[49],"preparation.":[50],"It":[51],"can":[52],"adversely":[53],"affect":[54],"the":[55,58,115],"reliability":[56],"results.":[59],"Here,":[60],"we":[61,145],"present":[62],"modified":[64],"concept":[65],"quantification":[68],"in":[69,114,140,157],"adherent":[70,92,158],"cell":[71,84,93,134,159],"lines":[72,94],"FCM":[74],"involves":[76],"neither":[77],"nor":[80],"any":[81],"transferring":[85],"steps.":[86],"Our":[87],"high":[88],"throughput":[89],"assay":[90],"reduces":[95],"sample-processing":[96],"time,":[97],"requires":[98],"minimal":[99],"workload,":[100],"provides":[101],"possibility":[103],"for":[104,133,154],"automation,":[105],"and,":[106],"if":[107],"needed,":[108],"also":[109],"allows":[110],"significant":[112],"reduction":[113],"size":[116],"individual":[118],"samples.":[119],"Working":[120],"well-proven":[123],"commercial":[124],"tool-The":[125],"BD":[126],"Cycletest\u2122":[127],"Plus":[128],"Reagent":[130],"Kit-primarily":[131],"designed":[132],"cycle":[135],"aneuploidy":[138],"determination":[139],"experimental":[141],"clinical":[143],"samples,":[144],"suggest":[146],"novel,":[148],"very":[149],"efficient,":[150],"robust":[152],"approach":[153],"research":[156],"cultures.":[160]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2024-05-05T00:00:00"}