{"id":"https://openalex.org/W4394714871","doi":"https://doi.org/10.1016/j.crmeth.2024.100756","title":"Mutational rescue of the activity of high-fidelity Cas9 enzymes","display_name":"Mutational rescue of the activity of high-fidelity Cas9 enzymes","publication_year":2024,"publication_date":"2024-04-01","ids":{"openalex":"https://openalex.org/W4394714871","doi":"https://doi.org/10.1016/j.crmeth.2024.100756","pmid":"https://pubmed.ncbi.nlm.nih.gov/38608689","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/11046035"},"language":"en","primary_location":{"id":"doi:10.1016/j.crmeth.2024.100756","doi":"https://doi.org/10.1016/j.crmeth.2024.100756","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.crmeth.2024.100756","pdf_url":null,"source":{"id":"https://openalex.org/S4210214705","display_name":"Cell Reports Methods","issn_l":"2667-2375","issn":["2667-2375"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell Reports Methods","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.1016/j.crmeth.2024.100756","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5052274545","display_name":"Pascal D. Vos","orcid":"https://orcid.org/0000-0002-5770-8283","observed_orcids":["https://orcid.org/0000-0002-5770-8283"]},"institutions":[{"id":"https://openalex.org/I205640436","display_name":"Curtin University","ror":"https://ror.org/02n415q13","country_code":"AU","type":"education","lineage":["https://openalex.org/I205640436"]},{"id":"https://openalex.org/I2799971621","display_name":"Harry Perkins Institute of Medical Research","ror":"https://ror.org/02xz7d723","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I2799971621"]},{"id":"https://openalex.org/I4210088912","display_name":"Queen Elizabeth II Medical Centre","ror":"https://ror.org/0071a2k97","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I4210088912"]},{"id":"https://openalex.org/I4210118210","display_name":"ARC Centre of Excellence in Synthetic Biology","ror":"https://ror.org/01p2zg436","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1337719021","https://openalex.org/I2801453606","https://openalex.org/I4210118210","https://openalex.org/I4210132349","https://openalex.org/I99043593"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Pascal D. Vos","raw_affiliation_strings":["Curtin Medical School, Curtin University, Bentley, WA 6102, Australia; Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia; Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia; ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia","Curtin Medical School, Curtin University, Bentley, WA 6102, Australia","Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Curtin Medical School, Curtin University, Bentley, WA 6102, Australia; Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia; Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia; ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I205640436","https://openalex.org/I2799971621","https://openalex.org/I4210088912","https://openalex.org/I4210118210"]},{"raw_affiliation_string":"ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I4210088912","https://openalex.org/I4210118210"]},{"raw_affiliation_string":"Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia","institution_ids":["https://openalex.org/I205640436"]},{"raw_affiliation_string":"Curtin Medical School, Curtin University, Bentley, WA 6102, Australia","institution_ids":["https://openalex.org/I205640436"]},{"raw_affiliation_string":"Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I2799971621","https://openalex.org/I4210088912"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082221111","display_name":"Andrianto P. Gandadireja","orcid":"https://orcid.org/0000-0002-1847-2079","observed_orcids":["https://orcid.org/0000-0002-1847-2079"]},"institutions":[{"id":"https://openalex.org/I205640436","display_name":"Curtin University","ror":"https://ror.org/02n415q13","country_code":"AU","type":"education","lineage":["https://openalex.org/I205640436"]},{"id":"https://openalex.org/I2799971621","display_name":"Harry Perkins Institute of Medical Research","ror":"https://ror.org/02xz7d723","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I2799971621"]},{"id":"https://openalex.org/I4210088912","display_name":"Queen Elizabeth II Medical Centre","ror":"https://ror.org/0071a2k97","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I4210088912"]},{"id":"https://openalex.org/I4210118210","display_name":"ARC Centre of Excellence in Synthetic Biology","ror":"https://ror.org/01p2zg436","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1337719021","https://openalex.org/I2801453606","https://openalex.org/I4210118210","https://openalex.org/I4210132349","https://openalex.org/I99043593"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Andrianto P. Gandadireja","raw_affiliation_strings":["Curtin Medical School, Curtin University, Bentley, WA 6102, Australia; Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia; Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia; ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia","Curtin Medical School, Curtin University, Bentley, WA 6102, Australia","Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Curtin Medical School, Curtin University, Bentley, WA 6102, Australia; Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia; Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia; ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I205640436","https://openalex.org/I2799971621","https://openalex.org/I4210088912","https://openalex.org/I4210118210"]},{"raw_affiliation_string":"ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I4210088912","https://openalex.org/I4210118210"]},{"raw_affiliation_string":"Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia","institution_ids":["https://openalex.org/I205640436"]},{"raw_affiliation_string":"Curtin Medical School, Curtin University, Bentley, WA 6102, Australia","institution_ids":["https://openalex.org/I205640436"]},{"raw_affiliation_string":"Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I2799971621","https://openalex.org/I4210088912"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101579227","display_name":"Giulia Rossetti","orcid":"https://orcid.org/0000-0002-7502-1768","observed_orcids":["https://orcid.org/0000-0002-7502-1768"]},"institutions":[{"id":"https://openalex.org/I175234967","display_name":"The Kids Research Institute Australia","ror":"https://ror.org/01dbmzx78","country_code":"AU","type":"nonprofit","lineage":["https://openalex.org/I175234967"]},{"id":"https://openalex.org/I177877127","display_name":"The University of Western Australia","ror":"https://ror.org/047272k79","country_code":"AU","type":"education","lineage":["https://openalex.org/I177877127"]},{"id":"https://openalex.org/I2799971621","display_name":"Harry Perkins Institute of Medical Research","ror":"https://ror.org/02xz7d723","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I2799971621"]},{"id":"https://openalex.org/I2800419323","display_name":"Princess Margaret Hospital for Children","ror":"https://ror.org/04ec98z42","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I2799506148","https://openalex.org/I2800419323","https://openalex.org/I2800882159","https://openalex.org/I4387155440"]},{"id":"https://openalex.org/I4210088912","display_name":"Queen Elizabeth II Medical Centre","ror":"https://ror.org/0071a2k97","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I4210088912"]},{"id":"https://openalex.org/I4210118210","display_name":"ARC Centre of Excellence in Synthetic Biology","ror":"https://ror.org/01p2zg436","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1337719021","https://openalex.org/I2801453606","https://openalex.org/I4210118210","https://openalex.org/I4210132349","https://openalex.org/I99043593"]},{"id":"https://openalex.org/I4387155440","display_name":"Perth Children's Hospital","ror":"https://ror.org/015zx6n37","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I4387155440"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Giulia Rossetti","raw_affiliation_strings":["Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia; ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia; Centre for Medical Research, The University of Western Australia, Nedlands, WA 6009, Australia; Telethon Kids Institute, Northern Entrance, Perth Children's Hospital, 15 Hospital Avenue, Nedlands, WA 6009, Australia","ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","Centre for Medical Research, The University of Western Australia, Nedlands, WA 6009, Australia","Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia","Telethon Kids Institute, Northern Entrance, Perth Children's Hospital, 15 Hospital Avenue, Nedlands, WA 6009, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia; ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia; Centre for Medical Research, The University of Western Australia, Nedlands, WA 6009, Australia; Telethon Kids Institute, Northern Entrance, Perth Children's Hospital, 15 Hospital Avenue, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I175234967","https://openalex.org/I177877127","https://openalex.org/I2799971621","https://openalex.org/I4210088912","https://openalex.org/I4210118210","https://openalex.org/I4387155440"]},{"raw_affiliation_string":"ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I4210088912","https://openalex.org/I4210118210"]},{"raw_affiliation_string":"Centre for Medical Research, The University of Western Australia, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I177877127"]},{"raw_affiliation_string":"Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I2799971621","https://openalex.org/I4210088912"]},{"raw_affiliation_string":"Telethon Kids Institute, Northern Entrance, Perth Children's Hospital, 15 Hospital Avenue, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I175234967","https://openalex.org/I2800419323","https://openalex.org/I4387155440"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5003589882","display_name":"Stefan J. Siira","orcid":"https://orcid.org/0000-0002-8391-0143","observed_orcids":["https://orcid.org/0000-0002-8391-0143"]},"institutions":[{"id":"https://openalex.org/I175234967","display_name":"The Kids Research Institute Australia","ror":"https://ror.org/01dbmzx78","country_code":"AU","type":"nonprofit","lineage":["https://openalex.org/I175234967"]},{"id":"https://openalex.org/I177877127","display_name":"The University of Western Australia","ror":"https://ror.org/047272k79","country_code":"AU","type":"education","lineage":["https://openalex.org/I177877127"]},{"id":"https://openalex.org/I205640436","display_name":"Curtin University","ror":"https://ror.org/02n415q13","country_code":"AU","type":"education","lineage":["https://openalex.org/I205640436"]},{"id":"https://openalex.org/I2800419323","display_name":"Princess Margaret Hospital for Children","ror":"https://ror.org/04ec98z42","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I2799506148","https://openalex.org/I2800419323","https://openalex.org/I2800882159","https://openalex.org/I4387155440"]},{"id":"https://openalex.org/I4210088912","display_name":"Queen Elizabeth II Medical Centre","ror":"https://ror.org/0071a2k97","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I4210088912"]},{"id":"https://openalex.org/I4210118210","display_name":"ARC Centre of Excellence in Synthetic Biology","ror":"https://ror.org/01p2zg436","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1337719021","https://openalex.org/I2801453606","https://openalex.org/I4210118210","https://openalex.org/I4210132349","https://openalex.org/I99043593"]},{"id":"https://openalex.org/I4387155440","display_name":"Perth Children's Hospital","ror":"https://ror.org/015zx6n37","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I4387155440"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Stefan J. Siira","raw_affiliation_strings":["Curtin Medical School, Curtin University, Bentley, WA 6102, Australia; ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia; Centre for Medical Research, The University of Western Australia, Nedlands, WA 6009, Australia; Telethon Kids Institute, Northern Entrance, Perth Children's Hospital, 15 Hospital Avenue, Nedlands, WA 6009, Australia","ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","Centre for Medical Research, The University of Western Australia, Nedlands, WA 6009, Australia","Curtin Medical School, Curtin University, Bentley, WA 6102, Australia","Telethon Kids Institute, Northern Entrance, Perth Children's Hospital, 15 Hospital Avenue, Nedlands, WA 6009, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Curtin Medical School, Curtin University, Bentley, WA 6102, Australia; ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia; Centre for Medical Research, The University of Western Australia, Nedlands, WA 6009, Australia; Telethon Kids Institute, Northern Entrance, Perth Children's Hospital, 15 Hospital Avenue, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I175234967","https://openalex.org/I177877127","https://openalex.org/I205640436","https://openalex.org/I4210088912","https://openalex.org/I4210118210","https://openalex.org/I4387155440"]},{"raw_affiliation_string":"ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I4210088912","https://openalex.org/I4210118210"]},{"raw_affiliation_string":"Centre for Medical Research, The University of Western Australia, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I177877127"]},{"raw_affiliation_string":"Curtin Medical School, Curtin University, Bentley, WA 6102, Australia","institution_ids":["https://openalex.org/I205640436"]},{"raw_affiliation_string":"Telethon Kids Institute, Northern Entrance, Perth Children's Hospital, 15 Hospital Avenue, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I175234967","https://openalex.org/I2800419323","https://openalex.org/I4387155440"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027154660","display_name":"Jessica L. Mantegna","orcid":"https://orcid.org/0000-0002-4647-619X","observed_orcids":["https://orcid.org/0000-0002-4647-619X"]},"institutions":[{"id":"https://openalex.org/I205640436","display_name":"Curtin University","ror":"https://ror.org/02n415q13","country_code":"AU","type":"education","lineage":["https://openalex.org/I205640436"]},{"id":"https://openalex.org/I2799971621","display_name":"Harry Perkins Institute of Medical Research","ror":"https://ror.org/02xz7d723","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I2799971621"]},{"id":"https://openalex.org/I4210088912","display_name":"Queen Elizabeth II Medical Centre","ror":"https://ror.org/0071a2k97","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I4210088912"]},{"id":"https://openalex.org/I4210118210","display_name":"ARC Centre of Excellence in Synthetic Biology","ror":"https://ror.org/01p2zg436","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1337719021","https://openalex.org/I2801453606","https://openalex.org/I4210118210","https://openalex.org/I4210132349","https://openalex.org/I99043593"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Jessica L. Mantegna","raw_affiliation_strings":["Curtin Medical School, Curtin University, Bentley, WA 6102, Australia; Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia; Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia; ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia","Curtin Medical School, Curtin University, Bentley, WA 6102, Australia","Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Curtin Medical School, Curtin University, Bentley, WA 6102, Australia; Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia; Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia; ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I205640436","https://openalex.org/I2799971621","https://openalex.org/I4210088912","https://openalex.org/I4210118210"]},{"raw_affiliation_string":"ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I4210088912","https://openalex.org/I4210118210"]},{"raw_affiliation_string":"Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia","institution_ids":["https://openalex.org/I205640436"]},{"raw_affiliation_string":"Curtin Medical School, Curtin University, Bentley, WA 6102, Australia","institution_ids":["https://openalex.org/I205640436"]},{"raw_affiliation_string":"Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I2799971621","https://openalex.org/I4210088912"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5050949179","display_name":"Aleksandra Filipovska","orcid":"https://orcid.org/0000-0002-6998-8403","observed_orcids":["https://orcid.org/0000-0002-6998-8403"]},"institutions":[{"id":"https://openalex.org/I175234967","display_name":"The Kids Research Institute Australia","ror":"https://ror.org/01dbmzx78","country_code":"AU","type":"nonprofit","lineage":["https://openalex.org/I175234967"]},{"id":"https://openalex.org/I177877127","display_name":"The University of Western Australia","ror":"https://ror.org/047272k79","country_code":"AU","type":"education","lineage":["https://openalex.org/I177877127"]},{"id":"https://openalex.org/I2800419323","display_name":"Princess Margaret Hospital for Children","ror":"https://ror.org/04ec98z42","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I2799506148","https://openalex.org/I2800419323","https://openalex.org/I2800882159","https://openalex.org/I4387155440"]},{"id":"https://openalex.org/I4210088912","display_name":"Queen Elizabeth II Medical Centre","ror":"https://ror.org/0071a2k97","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I4210088912"]},{"id":"https://openalex.org/I4210118210","display_name":"ARC Centre of Excellence in Synthetic Biology","ror":"https://ror.org/01p2zg436","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1337719021","https://openalex.org/I2801453606","https://openalex.org/I4210118210","https://openalex.org/I4210132349","https://openalex.org/I99043593"]},{"id":"https://openalex.org/I4387155440","display_name":"Perth Children's Hospital","ror":"https://ror.org/015zx6n37","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I4387155440"]}],"countries":["AU"],"is_corresponding":false,"raw_author_name":"Aleksandra Filipovska","raw_affiliation_strings":["ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia; Centre for Medical Research, The University of Western Australia, Nedlands, WA 6009, Australia; Telethon Kids Institute, Northern Entrance, Perth Children's Hospital, 15 Hospital Avenue, Nedlands, WA 6009, Australia","ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","Centre for Medical Research, The University of Western Australia, Nedlands, WA 6009, Australia","Telethon Kids Institute, Northern Entrance, Perth Children's Hospital, 15 Hospital Avenue, Nedlands, WA 6009, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia; Centre for Medical Research, The University of Western Australia, Nedlands, WA 6009, Australia; Telethon Kids Institute, Northern Entrance, Perth Children's Hospital, 15 Hospital Avenue, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I175234967","https://openalex.org/I177877127","https://openalex.org/I2800419323","https://openalex.org/I4210088912","https://openalex.org/I4210118210","https://openalex.org/I4387155440"]},{"raw_affiliation_string":"ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I4210088912","https://openalex.org/I4210118210"]},{"raw_affiliation_string":"Centre for Medical Research, The University of Western Australia, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I177877127"]},{"raw_affiliation_string":"Telethon Kids Institute, Northern Entrance, Perth Children's Hospital, 15 Hospital Avenue, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I175234967","https://openalex.org/I2800419323","https://openalex.org/I4387155440"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5070261696","display_name":"Oliver Rackham","orcid":"https://orcid.org/0000-0002-5301-9624","observed_orcids":["https://orcid.org/0000-0002-5301-9624"]},"institutions":[{"id":"https://openalex.org/I175234967","display_name":"The Kids Research Institute Australia","ror":"https://ror.org/01dbmzx78","country_code":"AU","type":"nonprofit","lineage":["https://openalex.org/I175234967"]},{"id":"https://openalex.org/I205640436","display_name":"Curtin University","ror":"https://ror.org/02n415q13","country_code":"AU","type":"education","lineage":["https://openalex.org/I205640436"]},{"id":"https://openalex.org/I2799971621","display_name":"Harry Perkins Institute of Medical Research","ror":"https://ror.org/02xz7d723","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I2799971621"]},{"id":"https://openalex.org/I2800419323","display_name":"Princess Margaret Hospital for Children","ror":"https://ror.org/04ec98z42","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I2799506148","https://openalex.org/I2800419323","https://openalex.org/I2800882159","https://openalex.org/I4387155440"]},{"id":"https://openalex.org/I4210088912","display_name":"Queen Elizabeth II Medical Centre","ror":"https://ror.org/0071a2k97","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I4210088912"]},{"id":"https://openalex.org/I4210118210","display_name":"ARC Centre of Excellence in Synthetic Biology","ror":"https://ror.org/01p2zg436","country_code":"AU","type":"facility","lineage":["https://openalex.org/I1337719021","https://openalex.org/I2801453606","https://openalex.org/I4210118210","https://openalex.org/I4210132349","https://openalex.org/I99043593"]},{"id":"https://openalex.org/I4387155440","display_name":"Perth Children's Hospital","ror":"https://ror.org/015zx6n37","country_code":"AU","type":"healthcare","lineage":["https://openalex.org/I4387155440"]}],"countries":["AU"],"is_corresponding":true,"raw_author_name":"Oliver Rackham","raw_affiliation_strings":["Curtin Medical School, Curtin University, Bentley, WA 6102, Australia; Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia; Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia; ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia; Telethon Kids Institute, Northern Entrance, Perth Children's Hospital, 15 Hospital Avenue, Nedlands, WA 6009, Australia. Electronic address: oliver.rackham@curtin.edu.au","ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia","Curtin Medical School, Curtin University, Bentley, WA 6102, Australia","Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia","Telethon Kids Institute, Northern Entrance, Perth Children's Hospital, 15 Hospital Avenue, Nedlands, WA 6009, Australia"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Curtin Medical School, Curtin University, Bentley, WA 6102, Australia; Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia; Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia; ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia; Telethon Kids Institute, Northern Entrance, Perth Children's Hospital, 15 Hospital Avenue, Nedlands, WA 6009, Australia. Electronic address: oliver.rackham@curtin.edu.au","institution_ids":["https://openalex.org/I175234967","https://openalex.org/I205640436","https://openalex.org/I2799971621","https://openalex.org/I4210088912","https://openalex.org/I4387155440"]},{"raw_affiliation_string":"ARC Centre of Excellence in Synthetic Biology, QEII Medical Centre, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I4210088912","https://openalex.org/I4210118210"]},{"raw_affiliation_string":"Curtin Health Innovation Research Institute, Curtin University, Bentley, WA 6102, Australia","institution_ids":["https://openalex.org/I205640436"]},{"raw_affiliation_string":"Curtin Medical School, Curtin University, Bentley, WA 6102, Australia","institution_ids":["https://openalex.org/I205640436"]},{"raw_affiliation_string":"Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I2799971621","https://openalex.org/I4210088912"]},{"raw_affiliation_string":"Telethon Kids Institute, Northern Entrance, Perth Children's Hospital, 15 Hospital Avenue, Nedlands, WA 6009, Australia","institution_ids":["https://openalex.org/I175234967","https://openalex.org/I2800419323","https://openalex.org/I4387155440"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":8,"corresponding_author_ids":["https://openalex.org/A5070261696"],"corresponding_institution_ids":["https://openalex.org/I175234967","https://openalex.org/I205640436","https://openalex.org/I2799971621","https://openalex.org/I2800419323","https://openalex.org/I4210088912","https://openalex.org/I4210118210","https://openalex.org/I4387155440"],"apc_list":{"value":5200,"currency":"USD","value_usd":5200},"apc_paid":{"value":5200,"currency":"USD","value_usd":5200},"fwci":1.3031,"has_fulltext":true,"cited_by_count":12,"citation_normalized_percentile":{"value":0.78103197,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":98},"biblio":{"volume":"4","issue":"4","first_page":"100756","last_page":"100756"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12610","display_name":"RNA regulation and disease","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11491","display_name":"CAR-T cell therapy research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cas9","display_name":"Cas9","score":0.7039999961853027},{"id":"https://openalex.org/keywords/crispr-cas9","display_name":"CRISPR/Cas9","score":0.9190000295639038},{"id":"https://openalex.org/keywords/off-target-effects","display_name":"off-target effects","score":0.6050000190734863},{"id":"https://openalex.org/keywords/genome-editing","display_name":"genome editing","score":0.7129999995231628},{"id":"https://openalex.org/keywords/mammalian-cells","display_name":"mammalian cells","score":0.5400000214576721},{"id":"https://openalex.org/keywords/insertion-deletion-mutations","display_name":"insertion-deletion mutations","score":0.5839999914169312}],"concepts":[{"id":"https://openalex.org/C98108389","wikidata":"https://www.wikidata.org/wiki/Q412563","display_name":"CRISPR","level":3,"score":0.8640527725219727},{"id":"https://openalex.org/C144501496","wikidata":"https://www.wikidata.org/wiki/Q5533489","display_name":"Genome editing","level":4,"score":0.8460155129432678},{"id":"https://openalex.org/C132455925","wikidata":"https://www.wikidata.org/wiki/Q16965677","display_name":"Cas9","level":4,"score":0.8257954716682434},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.6354615092277527},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5223561525344849},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5183554887771606},{"id":"https://openalex.org/C197129107","wikidata":"https://www.wikidata.org/wiki/Q1921621","display_name":"Merge (version control)","level":2,"score":0.4565200209617615},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4393481910228729},{"id":"https://openalex.org/C2777028655","wikidata":"https://www.wikidata.org/wiki/Q415601","display_name":"Endonuclease","level":3,"score":0.4268520772457123},{"id":"https://openalex.org/C2776459999","wikidata":"https://www.wikidata.org/wiki/Q2119376","display_name":"Fidelity","level":2,"score":0.4123813211917877},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.33275914192199707},{"id":"https://openalex.org/C23123220","wikidata":"https://www.wikidata.org/wiki/Q816826","display_name":"Information retrieval","level":1,"score":0.0},{"id":"https://openalex.org/C76155785","wikidata":"https://www.wikidata.org/wiki/Q418","display_name":"Telecommunications","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000072669","descriptor_name":"Gene Editing","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D000076987","descriptor_name":"CRISPR-Associated Protein 9","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000076987","descriptor_name":"CRISPR-Associated Protein 9","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004720","descriptor_name":"Endonucleases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004720","descriptor_name":"Endonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D057809","descriptor_name":"HEK293 Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D064113","descriptor_name":"CRISPR-Cas Systems","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1016/j.crmeth.2024.100756","doi":"https://doi.org/10.1016/j.crmeth.2024.100756","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.crmeth.2024.100756","pdf_url":null,"source":{"id":"https://openalex.org/S4210214705","display_name":"Cell Reports Methods","issn_l":"2667-2375","issn":["2667-2375"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell Reports Methods","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:11046035","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/11046035","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC11046035/pdf/main.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell Rep Methods","raw_type":"Text"},{"id":"pmid:38608689","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/38608689","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell reports methods","raw_type":"Journal Article"},{"id":"pmh:oai:pure.atira.dk:openaire/e0c33cdc-8343-45d7-9a73-4e3d0dc5c61a","doi":null,"is_oa":true,"landing_page_url":"https://admin.research-repository.uwa.edu.au/en/publications/e0c33cdc-8343-45d7-9a73-4e3d0dc5c61a","pdf_url":null,"source":{"id":"https://openalex.org/S4306402492","display_name":"UWA Profiles and Research Repository (UWA)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I177877127","host_organization_name":"The University of Western Australia","host_organization_lineage":["https://openalex.org/I177877127"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Vos, P D, Gandadireja, A P, Rossetti, G, Siira, S J, Mantegna, J L, Filipovska, A & Rackham, O 2024, 'Mutational rescue of the activity of high-fidelity Cas9 enzymes', Cell Reports Methods, vol. 4, no. 4, 100756. https://doi.org/10.1016/j.crmeth.2024.100756","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"doi:10.1016/j.crmeth.2024.100756","doi":"https://doi.org/10.1016/j.crmeth.2024.100756","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.crmeth.2024.100756","pdf_url":null,"source":{"id":"https://openalex.org/S4210214705","display_name":"Cell Reports Methods","issn_l":"2667-2375","issn":["2667-2375"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","oarl","poland-20"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cell Reports Methods","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.4043000042438507,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G2082644232","display_name":"Engineering improved and multifunctional gene editing systems","funder_award_id":"DP210103816","funder_id":"https://openalex.org/F4320334704","funder_display_name":"Australian Research Council"},{"id":"https://openalex.org/G2182782424","display_name":"Designing new therapeutics using medical synthetic biology","funder_award_id":"1154932","funder_id":"https://openalex.org/F4320334705","funder_display_name":"National Health and Medical Research Council"},{"id":"https://openalex.org/G833214861","display_name":"Targeting mitochondrial dysfunction in cardiovascular disease and cancer","funder_award_id":"1154646","funder_id":"https://openalex.org/F4320334705","funder_display_name":"National Health and Medical Research Council"},{"id":"https://openalex.org/G8811412286","display_name":"ARC Centre of Excellence in Synthetic Biology","funder_award_id":"CE200100029","funder_id":"https://openalex.org/F4320334704","funder_display_name":"Australian Research Council"}],"funders":[{"id":"https://openalex.org/F4320315074","display_name":"Australian Genome Research Facility","ror":"https://ror.org/053v53919"},{"id":"https://openalex.org/F4320320991","display_name":"University of Western Australia","ror":"https://ror.org/047272k79"},{"id":"https://openalex.org/F4320334704","display_name":"Australian Research Council","ror":"https://ror.org/05mmh0f86"},{"id":"https://openalex.org/F4320334705","display_name":"National Health and Medical Research Council","ror":"https://ror.org/011kf5r70"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4394714871.pdf","grobid_xml":"https://content.openalex.org/works/W4394714871.grobid-xml"},"referenced_works_count":35,"referenced_works":["https://openalex.org/W2003797386","https://openalex.org/W2036897871","https://openalex.org/W2077659966","https://openalex.org/W2131271579","https://openalex.org/W2136145671","https://openalex.org/W2157107905","https://openalex.org/W2188223075","https://openalex.org/W2224398381","https://openalex.org/W2346279274","https://openalex.org/W2554763217","https://openalex.org/W2726053863","https://openalex.org/W2736253687","https://openalex.org/W2766354636","https://openalex.org/W2766874121","https://openalex.org/W2781931843","https://openalex.org/W2787206763","https://openalex.org/W2793309516","https://openalex.org/W2886819502","https://openalex.org/W2896098390","https://openalex.org/W2953019923","https://openalex.org/W2961875637","https://openalex.org/W2985045444","https://openalex.org/W3006116138","https://openalex.org/W3009572786","https://openalex.org/W3010215023","https://openalex.org/W3011375666","https://openalex.org/W3034029360","https://openalex.org/W3042624753","https://openalex.org/W3153102876","https://openalex.org/W3214328950","https://openalex.org/W4214881544","https://openalex.org/W4245053291","https://openalex.org/W4281757975","https://openalex.org/W4307891585","https://openalex.org/W6780370582"],"related_works":["https://openalex.org/W4281757975","https://openalex.org/W4324129494","https://openalex.org/W2726053863","https://openalex.org/W3003386920","https://openalex.org/W3007126393","https://openalex.org/W3137982617","https://openalex.org/W2115495815","https://openalex.org/W2952406481","https://openalex.org/W2979046344","https://openalex.org/W2224398381"],"abstract_inverted_index":{"Programmable":[0],"DNA":[1],"endonucleases":[2],"derived":[3],"from":[4],"bacterial":[5],"genetic":[6],"defense":[7],"systems,":[8],"exemplified":[9],"by":[10],"CRISPR-Cas9,":[11],"have":[12,29,56],"made":[13],"it":[14],"significantly":[15],"easier":[16],"to":[17,59,83],"perform":[18],"genomic":[19,113],"modifications":[20,27],"in":[21,102,132],"living":[22],"cells.":[23],"However,":[24],"unprogrammed,":[25],"off-target":[26,119],"can":[28],"serious":[30],"consequences,":[31],"as":[32],"they":[33],"often":[34],"disrupt":[35],"the":[36,45,74,93],"function":[37],"or":[38],"regulation":[39],"of":[40,47,54,76,96],"non-targeted":[41],"genes":[42],"and":[43,79,105,108,125,134],"compromise":[44],"safety":[46],"therapeutic":[48],"gene":[49,63,129],"editing":[50,130],"applications.":[51],"High-fidelity":[52],"mutants":[53],"Cas9":[55,78,81],"been":[57],"established":[58],"enable":[60],"more":[61],"accurate":[62],"editing,":[64],"but":[65],"these":[66],"are":[67],"typically":[68],"less":[69],"efficient.":[70],"Here,":[71],"we":[72,88],"merge":[73],"strengths":[75],"high-fidelity":[77],"hyperactive":[80],"variants":[82],"provide":[84],"an":[85],"enzyme,":[86],"which":[87],"dub":[89],"HyperDriveCas9,":[90],"that":[91],"yields":[92],"desirable":[94],"properties":[95],"both":[97],"parents.":[98],"HyperDriveCas9":[99,121],"functions":[100],"efficiently":[101],"mammalian":[103],"cells":[104],"introduces":[106],"insertion":[107],"deletion":[109],"mutations":[110],"into":[111],"targeted":[112],"regions":[114],"while":[115],"maintaining":[116],"a":[117,123],"favorable":[118],"profile.":[120],"is":[122],"precise":[124],"efficient":[126],"tool":[127],"for":[128],"applications":[131],"science":[133],"medicine.":[135]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":2}],"updated_date":"2026-11-12T12:28:57.059296","created_date":"2024-04-12T00:00:00"}