{"id":"https://openalex.org/W4394544549","doi":"https://doi.org/10.6084/m9.figshare.5121469","title":"Supplementary Material for: High Efficiency of a Sequential Recombinase-Mediated Cassette Exchange Reaction in Escherichia coli","display_name":"Supplementary Material for: High Efficiency of a Sequential Recombinase-Mediated Cassette Exchange Reaction in Escherichia coli","publication_year":2010,"publication_date":"2010-01-01","ids":{"openalex":"https://openalex.org/W4394544549","doi":"https://doi.org/10.6084/m9.figshare.5121469"},"language":"en","primary_location":{"id":"pmh:oai:figshare.com:article/5121469","doi":null,"is_oa":true,"landing_page_url":"https://figshare.com/articles/dataset/Supplementary_Material_for_High_Efficiency_of_a_Sequential_Recombinase-Mediated_Cassette_Exchange_Reaction_in_i_Escherichia_coli_i_/5121469","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dataset"},"volume":null,"type":"supplementary-materials","is_traditional":false,"indexed_in":["datacite"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://figshare.com/articles/dataset/Supplementary_Material_for_High_Efficiency_of_a_Sequential_Recombinase-Mediated_Cassette_Exchange_Reaction_in_i_Escherichia_coli_i_/5121469","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5049154173","display_name":"Natalia Malchin","orcid":"https://orcid.org/0000-0003-1103-476X","observed_orcids":["https://orcid.org/0000-0003-1103-476X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Malchin, N.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073247691","display_name":"Tatiana Molotsky","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Molotsky, T.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5066847239","display_name":"Ilya Borovok","orcid":"https://orcid.org/0000-0003-1602-4499","observed_orcids":["https://orcid.org/0000-0003-1602-4499"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Borovok, I.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015175999","display_name":"Yuri Voziyanov","orcid":"https://orcid.org/0000-0003-0232-3105","observed_orcids":["https://orcid.org/0000-0003-0232-3105"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Voziyanov, Y.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035669040","display_name":"Alexander B. Kotlyar","orcid":"https://orcid.org/0000-0003-0713-6499","observed_orcids":["https://orcid.org/0000-0003-0713-6499"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kotlyar, A.B.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037697790","display_name":"Ezra Yagil","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yagil, E.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5060263553","display_name":"Mikhail N. Kolot","orcid":"https://orcid.org/0000-0002-3473-2790","observed_orcids":["https://orcid.org/0000-0002-3473-2790"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kolot, M.","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9355000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.9355000257492065,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.055799998342990875,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10123","display_name":"DNA Repair Mechanisms","score":0.005100000184029341,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/recombinase-mediated-cassette-exchange","display_name":"recombinase-mediated cassette exchange","score":0.9919999837875366},{"id":"https://openalex.org/keywords/flp-recombinase","display_name":"Flp recombinase","score":0.8830000162124634},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8669999837875366},{"id":"https://openalex.org/keywords/recombination-efficiency","display_name":"recombination efficiency","score":0.5989999771118164},{"id":"https://openalex.org/keywords/synaptic-complex","display_name":"synaptic complex","score":0.6949999928474426},{"id":"https://openalex.org/keywords/frt-sites","display_name":"FRT sites","score":0.9309999942779541},{"id":"https://openalex.org/keywords/site-specific-recombination","display_name":"site-specific recombination","score":0.824999988079071}],"concepts":[{"id":"https://openalex.org/C102414288","wikidata":"https://www.wikidata.org/wiki/Q1172461","display_name":"Recombinase","level":4,"score":0.6988965272903442},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.6879684925079346},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4536910653114319},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.34930509328842163},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3419922888278961},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2915979027748108},{"id":"https://openalex.org/C156695909","wikidata":"https://www.wikidata.org/wiki/Q3373825","display_name":"Recombination","level":3,"score":0.14273491501808167},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.07046744227409363}],"mesh":[],"locations_count":3,"locations":[{"id":"pmh:oai:figshare.com:article/5121469","doi":null,"is_oa":true,"landing_page_url":"https://figshare.com/articles/dataset/Supplementary_Material_for_High_Efficiency_of_a_Sequential_Recombinase-Mediated_Cassette_Exchange_Reaction_in_i_Escherichia_coli_i_/5121469","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dataset"},{"id":"doi:10.6084/m9.figshare.5121469","doi":"https://doi.org/10.6084/m9.figshare.5121469","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.5121469","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dataset"},{"id":"doi:10.6084/m9.figshare.5121469.v1","doi":"https://doi.org/10.6084/m9.figshare.5121469.v1","is_oa":true,"landing_page_url":"https://doi.org/10.6084/m9.figshare.5121469.v1","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"Dataset"}],"best_oa_location":{"id":"pmh:oai:figshare.com:article/5121469","doi":null,"is_oa":true,"landing_page_url":"https://figshare.com/articles/dataset/Supplementary_Material_for_High_Efficiency_of_a_Sequential_Recombinase-Mediated_Cassette_Exchange_Reaction_in_i_Escherichia_coli_i_/5121469","pdf_url":null,"source":{"id":"https://openalex.org/S4377196282","display_name":"Figshare","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210132348","host_organization_name":"Figshare (United Kingdom)","host_organization_lineage":["https://openalex.org/I4210132348"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Dataset"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2101502234","https://openalex.org/W2122033230","https://openalex.org/W2156913716","https://openalex.org/W1591997984","https://openalex.org/W2095463884","https://openalex.org/W2135607706","https://openalex.org/W2127633067","https://openalex.org/W4205719890","https://openalex.org/W2748671686","https://openalex.org/W1988797860"],"abstract_inverted_index":{"A":[0],"comparison":[1],"between":[2],"the":[3,16,61,70,88,113,119],"efficiency":[4],"of":[5,20,24,50,56,63,87,94,105],"recombinase-mediated":[6],"cassette":[7],"exchange":[8],"(RMCE)":[9],"reactions":[10],"catalyzed":[11,40,59,73],"in":[12,60,79],"Escherichia":[13],"coli":[14],"by":[15,74],"site-specific":[17],"recombinases":[18,65],"Flp":[19,95],"yeast":[21],"and":[22,53],"Int":[23],"coliphage":[25],"HK022":[26],"has":[27],"revealed":[28],"that":[29,101,118],"an":[30,38,44],"Flp-catalyzed":[31],"RMCE":[32,45,120],"reaction":[33,46,72,121],"is":[34,66,83,122],"more":[35],"efficient":[36],"than":[37],"Int-HK022":[39],"reaction.":[41],"In":[42],"contrast,":[43],"with":[47,96,112],"1":[48,54,103],"pair":[49,55,104],"frt":[51],"sites":[52,58,107],"att":[57],"presence":[62],"both":[64],"very":[67,84],"inefficient.":[68],"However,":[69],"same":[71],"each":[75],"recombinase":[76],"individually":[77],"supplied":[78],"a":[80,109],"sequential":[81],"order":[82],"efficient,":[85],"regardless":[86],"order.":[89],"Atomic":[90],"force":[91],"microscopy":[92],"images":[93],"its":[97],"DNA":[98],"substrates":[99],"show":[100],"only":[102],"recombination":[106],"forms":[108],"synaptic":[110],"complex":[111],"recombinase.":[114],"The":[115],"results":[116],"suggest":[117],"sequential.":[123]},"counts_by_year":[],"updated_date":"2026-10-08T09:06:37.294493","created_date":"2024-04-08T00:00:00"}