{"id":"https://openalex.org/W4393096020","doi":"https://doi.org/10.1158/1538-7445.am2024-3767","title":"Abstract 3767: A novel method to minimize HIER-induced alterations on H&E staining in an integrated mIF-H&E workflow","display_name":"Abstract 3767: A novel method to minimize HIER-induced alterations on H&E staining in an integrated mIF-H&E workflow","publication_year":2024,"publication_date":"2024-03-22","ids":{"openalex":"https://openalex.org/W4393096020","doi":"https://doi.org/10.1158/1538-7445.am2024-3767"},"language":"en","primary_location":{"id":"doi:10.1158/1538-7445.am2024-3767","doi":"https://doi.org/10.1158/1538-7445.am2024-3767","is_oa":false,"landing_page_url":"http://dx.doi.org/10.1158/1538-7445.am2024-3767","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","is_traditional":false,"indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101808657","display_name":"Kevin Hwang","orcid":"https://orcid.org/0000-0003-4210-0694","observed_orcids":["https://orcid.org/0000-0003-4210-0694"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kevin Hwang","raw_affiliation_strings":["1Ultivue, Inc., Cambridge, MA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Ultivue, Inc., Cambridge, MA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060525331","display_name":"Grace E. Vezeau","orcid":"https://orcid.org/0000-0001-6087-3338","observed_orcids":["https://orcid.org/0000-0001-6087-3338"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Grace Vezeau","raw_affiliation_strings":["1Ultivue, Inc., Cambridge, MA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Ultivue, Inc., Cambridge, MA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5094224783","display_name":"Edyta Olejnik","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Edyta Olejnik","raw_affiliation_strings":["1Ultivue, Inc., Cambridge, MA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Ultivue, Inc., Cambridge, MA","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"Douglas Wood","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Douglas Wood","raw_affiliation_strings":["1Ultivue, Inc., Cambridge, MA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Ultivue, Inc., Cambridge, MA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5009961099","display_name":"Rub\u00e9n C\u00e1rdenes","orcid":"https://orcid.org/0000-0002-3693-453X","observed_orcids":["https://orcid.org/0000-0002-3693-453X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Ruben Cardenes","raw_affiliation_strings":["1Ultivue, Inc., Cambridge, MA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Ultivue, Inc., Cambridge, MA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5094219186","display_name":"Lauren Duro","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lauren Duro","raw_affiliation_strings":["1Ultivue, Inc., Cambridge, MA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Ultivue, Inc., Cambridge, MA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5081639937","display_name":"Gourab Chatterjee","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Gourab Chatterjee","raw_affiliation_strings":["1Ultivue, Inc., Cambridge, MA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Ultivue, Inc., Cambridge, MA","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5045665190","display_name":"Je Hyuk Lee","orcid":"https://orcid.org/0000-0001-6947-7446","observed_orcids":["https://orcid.org/0000-0001-6947-7446"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Je H. Lee","raw_affiliation_strings":["1Ultivue, Inc., Cambridge, MA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Ultivue, Inc., Cambridge, MA","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"84","issue":"6_Supplement","first_page":"3767","last_page":"3767"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10862","display_name":"AI in cancer detection","score":0.21310000121593475,"subfield":{"id":"https://openalex.org/subfields/1702","display_name":"Artificial Intelligence"},"field":{"id":"https://openalex.org/fields/17","display_name":"Computer Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10862","display_name":"AI in cancer detection","score":0.21310000121593475,"subfield":{"id":"https://openalex.org/subfields/1702","display_name":"Artificial Intelligence"},"field":{"id":"https://openalex.org/fields/17","display_name":"Computer Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10158","display_name":"Cancer Immunotherapy and Biomarkers","score":0.19120000302791595,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T13921","display_name":"Biological Stains and Phytochemicals","score":0.09359999746084213,"subfield":{"id":"https://openalex.org/subfields/2734","display_name":"Pathology and Forensic Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/heat-induced-epitope-retrieval","display_name":"heat-induced epitope retrieval","score":0.8600000143051147},{"id":"https://openalex.org/keywords/multiplex-immunofluorescence","display_name":"multiplex immunofluorescence","score":0.9929999709129333},{"id":"https://openalex.org/keywords/hematoxylin-and-eosin","display_name":"hematoxylin and eosin","score":0.7559999823570251},{"id":"https://openalex.org/keywords/destaining","display_name":"destaining","score":0.4230000078678131},{"id":"https://openalex.org/keywords/co-registration","display_name":"co-registration","score":0.5120000243186951},{"id":"https://openalex.org/keywords/tissue-microenvironment","display_name":"tissue microenvironment","score":0.7160000205039978},{"id":"https://openalex.org/keywords/cell-density-quantification","display_name":"cell density quantification","score":0.6779999732971191},{"id":"https://openalex.org/keywords/cellular-phenotyping","display_name":"cellular phenotyping","score":0.5519999861717224},{"id":"https://openalex.org/keywords/tissue-architecture-preservation","display_name":"tissue architecture preservation","score":0.30799999833106995},{"id":"https://openalex.org/keywords/workflow-optimization","display_name":"workflow optimization","score":0.3100000023841858}],"concepts":[{"id":"https://openalex.org/C177212765","wikidata":"https://www.wikidata.org/wiki/Q627335","display_name":"Workflow","level":2,"score":0.6681568622589111},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4786173701286316},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.45183759927749634},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.360245943069458},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.25050199031829834},{"id":"https://openalex.org/C77088390","wikidata":"https://www.wikidata.org/wiki/Q8513","display_name":"Database","level":1,"score":0.16072458028793335},{"id":"https://openalex.org/C142724271","wikidata":"https://www.wikidata.org/wiki/Q7208","display_name":"Pathology","level":1,"score":0.14192569255828857}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1538-7445.am2024-3767","doi":"https://doi.org/10.1158/1538-7445.am2024-3767","is_oa":false,"landing_page_url":"http://dx.doi.org/10.1158/1538-7445.am2024-3767","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.6240000128746033,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3098281821","https://openalex.org/W4409631112","https://openalex.org/W4388042801","https://openalex.org/W4385851685","https://openalex.org/W4394848536","https://openalex.org/W4406086366","https://openalex.org/W7167578729","https://openalex.org/W4388085886","https://openalex.org/W7148899836","https://openalex.org/W4410392037"],"abstract_inverted_index":{"Abstract":[0],"Background:":[1],"Hematoxylin":[2],"and":[3,18,39,130,179,198,215,218,226,233,248,266,295,301,310,320,350,357,387],"eosin":[4,296],"staining":[5,87,193,239,316,411,441],"(H&E)":[6],"is":[7,347,351,379],"widely":[8,348],"used":[9,221,349],"as":[10,28,98],"an":[11,118,140,443],"anatomical":[12,52,356],"assay":[13,402],"for":[14,194,390,454],"clinical":[15],"diagnosis.":[16],"Researchers":[17],"clinicians":[19],"also":[20],"rely":[21],"on":[22,36,439],"molecular":[23,55],"in":[24,50,84,237,292,313,361,372,381,409,442],"situ":[25],"techniques,":[26,57],"such":[27],"multiplexed":[29,137],"immunofluorescence":[30,138],"(mIF),":[31],"to":[32,81,126,209,222,279,344,367,435],"gain":[33],"deeper":[34],"insights":[35],"cellular":[37,333,358],"phenotypes":[38],"tissue":[40,146,267,274],"microenvironment.":[41],"As":[42],"a":[43,62,71,114,151,156,162,170,185,252,259,336],"result,":[44],"there":[45],"has":[46,78],"been":[47,79],"significant":[48],"interest":[49],"combining":[51,117],"stains":[53],"with":[54,122,139,246,251,325],"imaging":[56],"most":[58,307,373],"commonly":[59],"by":[60,161],"using":[61,150,169,184,207,328],"terminal":[63,106,253],"H&E":[64,86,107,120,142,164,181,238,247,254,299,338,345,363,410,440],"stain":[65,108,121,182,364],"after":[66,109,255,281],"mIF":[67,158,192,230,261,304,315,343,374,391],"staining.":[68,305],"This":[69],"allows":[70],"combination":[72,400],"of":[73,102,104,134,201,353,370,401,450],"the":[74,85,99,105,180,202,342,362,368,397,406,451],"two":[75],"assays":[76],"but":[77,272,404],"observed":[80,241],"show":[82],"alterations":[83,103,129,360,408,438],"pattern.":[88,412],"In":[89],"this":[90],"work":[91],"we":[92],"identify":[93],"heat-induced":[94],"epitope":[95],"retrieval":[96],"(HIER)":[97],"root":[100],"cause":[101],"mIF.":[110,286],"We":[111],"further":[112],"demonstrate":[113],"new":[115],"workflow":[116,346,446],"initial":[119],"subsequent":[123,303],"InSituPlex":[124,314],"assay,":[125,159],"avoid":[127],"these":[128],"thus":[131],"enable":[132],"co-registration":[133],"highly":[135],"sensitive":[136],"unaltered":[141],"stain.":[143,165],"Methods:":[144],"FFPE":[145],"slides":[147,176,243,249,300,384],"were":[148,167,177,205,240,269,317,323],"stained":[149,245,250],"standard":[152,163],"HIER":[153,256,283,371],"protocol":[154,187,289],"or":[155,258,284,392],"full":[157,260,285],"followed":[160],"Slides":[166],"imaged":[168],"Zeiss":[171],"Axioscan.Z1":[172],"scanner.":[173],"Additional":[174],"H&E-stained":[175,383],"prepared,":[178],"removed":[183],"destaining":[186,288],"before":[188],"carrying":[189],"out":[190],"InSituPlex\u00ae":[191],"multiple":[195],"markers.":[196],"Fluorescence":[197],"brightfield":[199],"images":[200,322],"same":[203,398],"slide":[204],"overlaid":[206],"UltiStacker\u00ae":[208],"assess":[210],"qualitative":[211,309],"differences":[212,312],"between":[213,229,242],"pre-":[214,232],"post-mIF":[216],"H&E,":[217],"UltiAnalyzer.AI\u00ae":[219],"was":[220,290],"quantify":[223],"cell":[224,264],"densities":[225],"signal":[227],"intensities":[228],"image":[231],"post-H&E.":[234],"Results:":[235],"Alterations":[236],"directly":[244],"alone":[257],"assay.":[262],"Calculated":[263],"counts":[265],"area":[268],"consistent":[270],"throughout,":[271],"some":[273],"microstructures":[275],"could":[276],"be":[277],"difficult":[278],"resolve":[280],"either":[282],"Our":[287],"successful":[291],"removing":[293],"hematoxylin":[294],"from":[297],"directly-stained":[298],"allowing":[302,354],"For":[306],"biomarkers,":[308],"quantitative":[311],"minimal.":[318],"Brightfield":[319],"fluorescent":[321],"co-registered":[324],"sub-micron":[326],"accuracy":[327],"UltiStacker,":[329],"enabling":[330],"molecularly":[331],"defined":[332],"phenotyping":[334],"within":[335],"traditional":[337],"image.":[339],"Conclusions:":[340],"While":[341],"capable":[352],"combined":[355],"phenotyping,":[359],"exist":[365],"due":[366],"use":[369],"protocols.":[375],"An":[376],"alternative":[377],"method":[378,434],"possible":[380],"which":[382],"are":[385],"destained":[386],"then":[388],"restained":[389],"other":[393],"spatial":[394],"assays,":[395],"providing":[396],"valuable":[399],"information":[403],"without":[405],"HIER-induced":[407,437],"Citation":[413],"Format:":[414],"Kevin":[415],"Hwang,":[416],"Grace":[417],"Vezeau,":[418],"Edyta":[419],"Olejnik,":[420],"Douglas":[421],"Wood,":[422],"Ruben":[423],"Cardenes,":[424],"Lauren":[425],"Duro,":[426],"Gourab":[427],"Chatterjee,":[428],"Je":[429],"H.":[430],"Lee.":[431],"A":[432],"novel":[433],"minimize":[436],"integrated":[444],"mIF-H&E":[445],"[abstract].":[447],"In:":[448],"Proceedings":[449],"American":[452],"Association":[453],"Cancer":[455,473],"Research":[456],"Annual":[457],"Meeting":[458],"2024;":[459],"Part":[460],"1":[461],"(Regular":[462],"Abstracts);":[463],"2024":[464],"Apr":[465],"5-10;":[466],"San":[467],"Diego,":[468],"CA.":[469],"Philadelphia":[470],"(PA):":[471],"AACR;":[472],"Res":[474],"2024;84(6_Suppl):Abstract":[475],"nr":[476],"3767.":[477]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2024-03-23T00:00:00"}