{"id":"https://openalex.org/W4393095460","doi":"https://doi.org/10.1158/1538-7445.am2024-7486","title":"Abstract 7486: Development and analytic validation of an ARv7/PSMA dual biomarker circulating tumor cell assay","display_name":"Abstract 7486: Development and analytic validation of an ARv7/PSMA dual biomarker circulating tumor cell assay","publication_year":2024,"publication_date":"2024-03-22","ids":{"openalex":"https://openalex.org/W4393095460","doi":"https://doi.org/10.1158/1538-7445.am2024-7486"},"language":"en","primary_location":{"id":"doi:10.1158/1538-7445.am2024-7486","doi":"https://doi.org/10.1158/1538-7445.am2024-7486","is_oa":false,"landing_page_url":"http://dx.doi.org/10.1158/1538-7445.am2024-7486","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085355092","display_name":"Jon J. Ladd","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jon Ladd","raw_affiliation_strings":["1RareCyte, Inc., Seattle, WA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1RareCyte, Inc., Seattle, WA","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"Erin Bayer","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Erin Bayer","raw_affiliation_strings":["1RareCyte, Inc., Seattle, WA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1RareCyte, Inc., Seattle, WA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5094224118","display_name":"Brock Bartels","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Brock Bartels","raw_affiliation_strings":["1RareCyte, Inc., Seattle, WA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1RareCyte, Inc., Seattle, WA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5010999026","display_name":"Eric Peter Kaldjian","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Eric Kaldjian","raw_affiliation_strings":["1RareCyte, Inc., Seattle, WA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1RareCyte, Inc., Seattle, WA","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5112840706","display_name":"Arturo B. Ramirez","orcid":"https://orcid.org/0000-0001-8528-2569","observed_orcids":["https://orcid.org/0000-0001-8528-2569"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Arturo Ramirez","raw_affiliation_strings":["1RareCyte, Inc., Seattle, WA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1RareCyte, Inc., Seattle, WA","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"84","issue":"6_Supplement","first_page":"7486","last_page":"7486"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10543","display_name":"Prostate Cancer Treatment and Research","score":0.9101999998092651,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10543","display_name":"Prostate Cancer Treatment and Research","score":0.9101999998092651,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11287","display_name":"Cancer Genomics and Diagnostics","score":0.06589999794960022,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10124","display_name":"Prostate Cancer Diagnosis and Treatment","score":0.004800000227987766,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/circulating-tumor-cells","display_name":"circulating tumor cells","score":0.8389999866485596},{"id":"https://openalex.org/keywords/ar-v7","display_name":"AR-V7","score":0.8939999938011169},{"id":"https://openalex.org/keywords/prostate-specific-membrane-antigen","display_name":"prostate-specific membrane antigen","score":0.8389999866485596},{"id":"https://openalex.org/keywords/prostate-cancer","display_name":"prostate cancer","score":0.8769999742507935},{"id":"https://openalex.org/keywords/liquid-biopsy","display_name":"liquid biopsy","score":0.7519999742507935},{"id":"https://openalex.org/keywords/ar-splice-variants","display_name":"androgen receptor splice variants","score":0.7839999794960022},{"id":"https://openalex.org/keywords/androgen-receptor-antagonists","display_name":"androgen receptor antagonists","score":0.7440000176429749},{"id":"https://openalex.org/keywords/drug-resistance","display_name":"drug resistance","score":0.4959999918937683},{"id":"https://openalex.org/keywords/stage-iv-prostate-cancer","display_name":"stage IV prostate cancer","score":0.6940000057220459},{"id":"https://openalex.org/keywords/mean-fluorescence-intensity","display_name":"mean fluorescence intensity","score":0.5099999904632568},{"id":"https://openalex.org/keywords/epcam","display_name":"EpCAM","score":0.4230000078678131}],"concepts":[{"id":"https://openalex.org/C2781197716","wikidata":"https://www.wikidata.org/wiki/Q864574","display_name":"Biomarker","level":2,"score":0.7269994616508484},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.5819498300552368},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4196245074272156},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.40419095754623413},{"id":"https://openalex.org/C143998085","wikidata":"https://www.wikidata.org/wiki/Q162555","display_name":"Oncology","level":1,"score":0.3669184446334839},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.322989821434021},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2910715937614441},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.11476010084152222}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1538-7445.am2024-7486","doi":"https://doi.org/10.1158/1538-7445.am2024-7486","is_oa":false,"landing_page_url":"http://dx.doi.org/10.1158/1538-7445.am2024-7486","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9922999739646912,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[{"score":0.8299999833106995,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3083646176","https://openalex.org/W4393093280","https://openalex.org/W3093699595","https://openalex.org/W2887406141","https://openalex.org/W7167819683","https://openalex.org/W4392004791","https://openalex.org/W7148986187","https://openalex.org/W7162651857","https://openalex.org/W2954906007","https://openalex.org/W2972806868"],"abstract_inverted_index":{"Abstract":[0],"Background":[1],"Prostate":[2],"cancer":[3,80,282],"drug":[4,25],"targets":[5],"and":[6,30,51,61,110,139,147,154,159,165,168,201,228,256,266,311,326,357,364,386],"biomarkers":[7,243,349],"may":[8],"be":[9],"investigated":[10,275],"non-invasively":[11],"on":[12,65,174,197,306,350],"circulating":[13,394],"tumor":[14,395],"cells":[15,114,126,163],"(CTCs)":[16],"obtained":[17],"via":[18],"liquid":[19,368],"biopsies.":[20],"PSMA":[21,62,167,327,365],"is":[22,32,355],"an":[23,390],"important":[24],"target":[26],"in":[27,276,289,318,370],"prostate":[28,79,281],"cancer,":[29],"ARv7":[31,60,169,316,363],"a":[33,55,88,338],"splice":[34],"variant":[35],"that":[36,188,342],"confers":[37],"resistance":[38],"to":[39,58,81,121,151,156,170,324,332],"second-generation":[40],"androgen":[41],"receptor":[42],"inhibitors.":[43],"In":[44],"this":[45],"study,":[46],"we":[47],"describe":[48],"the":[49,134,182,216,219,272,360,402],"development":[50],"analytic":[52,224,387],"validation":[53,388],"of":[54,215,226,233,239,248,271,309,347,362,389,401],"novel":[56],"assay":[57,68,92,222,273,341,354,397],"quantify":[59],"protein":[63,91,172,345],"expression":[64,173,213,346,366],"CTCs.":[66,124,176,352],"The":[67,177,236,353],"was":[69,244,253,263,274],"validated":[70],"with":[71,76,149,166,181],"blood":[72,120,162,284],"samples":[73,132,292,320],"from":[74,129,322,330],"patients":[75],"Stage":[77,279],"IV":[78,280],"demonstrate":[82],"clinical":[83,291,319,371],"feasibility.":[84],"To":[85],"our":[86],"knowledge,":[87],"PSMA/ARv7":[89],"dual":[90,220,339,392],"for":[93,191,241,300,359,405],"CTCs":[94,158,190,286],"has":[95],"not":[96],"previously":[97],"been":[98],"reported.":[99],"Methods":[100],"LNCaP":[101],"(PSMA":[102,105],"positive),":[103,109],"PC3":[104],"negative),":[106],"22Rv1":[107],"(ARv7":[108,112],"BT474":[111],"negative)":[113],"were":[115,127,145,179,287,303],"spiked":[116],"into":[117],"healthy":[118],"donor":[119],"generate":[122],"model":[123,217],"Nucleated":[125],"collected":[128],"these":[130],"spike-in":[131],"using":[133],"AccuCyte\u00ae":[135],"sample":[136],"preparation":[137],"kit":[138],"spread":[140],"onto":[141],"microscope":[142,187],"slides.":[143],"Slides":[144],"fixed":[146],"stained":[148],"antibodies":[150],"cytokeratin,":[152],"EpCAM,":[153],"CD45":[155],"detect":[157],"exclude":[160],"white":[161],"(WBCs),":[164],"evaluate":[171],"identified":[175,288],"slides":[178],"imaged":[180],"CyteFinder\u00ae":[183],"automated":[184],"immunofluorescence":[185],"scanning":[186],"identifies":[189],"confirmation":[192],"by":[193,367],"trained":[194],"reviewers":[195],"based":[196,305],"machine":[198],"learning":[199],"algorithms":[200],"quantitatively":[202],"measures":[203,344],"biomarker":[204,221,301,393],"signal":[205],"mean":[206,261],"fluorescence":[207],"intensity":[208],"(MFI).":[209],"Results":[210],"For":[211],"ARv7/PSMA":[212,340,391],"classification":[214],"CTCs,":[218],"showed":[223],"sensitivity":[225],"83%/97%":[227],"specificity":[229],"98%/99%,":[230],"yielding":[231],"accuracy":[232],"90.2%/97.9%,":[234],"respectively.":[235],"dynamic":[237],"range":[238,295],"MFI":[240,307],"both":[242,348],"approximately":[245],"three":[246],"orders":[247],"magnitude.":[249],"Inter-run":[250],"reproducibility":[251],"CV":[252,262],"13.6%":[254],"(ARv7)":[255,265],"6.9%":[257],"(PSMA).":[258,268],"Slide":[259],"repeatability":[260],"11.1%":[264],"12.0%":[267],"Clinical":[269],"feasibility":[270],"9":[277],"progressing":[278],"patient":[283],"samples.":[285],"all":[290],"(median":[293],"4,":[294],"1":[296,412],"-":[297],"384).":[298],"Thresholds":[299],"positivity":[302,317,328],"established":[304],"measurements":[308],"positive":[310],"negative":[312],"control":[313],"cell":[314,396],"lines.":[315],"ranged":[321,329],"33%":[323],"100%,":[325],"0%":[331],"75%.":[333],"Conclusions":[334],"We":[335],"have":[336],"developed":[337],"simultaneously":[343],"individual":[351],"accurate":[356],"reproducible":[358],"assessment":[361],"biopsy":[369],"studies.":[372],"Citation":[373],"Format:":[374],"Jon":[375],"Ladd,":[376],"Erin":[377],"Bayer,":[378],"Brock":[379],"Bartels,":[380],"Eric":[381],"Kaldjian,":[382],"Arturo":[383],"Ramirez.":[384],"Development":[385],"[abstract].":[398],"In:":[399],"Proceedings":[400],"American":[403],"Association":[404],"Cancer":[406,424],"Research":[407],"Annual":[408],"Meeting":[409],"2024;":[410],"Part":[411],"(Regular":[413],"Abstracts);":[414],"2024":[415],"Apr":[416],"5-10;":[417],"San":[418],"Diego,":[419],"CA.":[420],"Philadelphia":[421],"(PA):":[422],"AACR;":[423],"Res":[425],"2024;84(6_Suppl):Abstract":[426],"nr":[427],"7486.":[428]},"counts_by_year":[],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2024-03-23T00:00:00"}