{"id":"https://openalex.org/W4393093633","doi":"https://doi.org/10.1158/1538-7445.am2024-3488","title":"Abstract 3488: Decoding splicing isoforms and adaptive evolution in melanoma brain metastases through single cell long-read RNA sequencing","display_name":"Abstract 3488: Decoding splicing isoforms and adaptive evolution in melanoma brain metastases through single cell long-read RNA sequencing","publication_year":2024,"publication_date":"2024-03-22","ids":{"openalex":"https://openalex.org/W4393093633","doi":"https://doi.org/10.1158/1538-7445.am2024-3488"},"language":"en","primary_location":{"id":"doi:10.1158/1538-7445.am2024-3488","doi":"https://doi.org/10.1158/1538-7445.am2024-3488","is_oa":false,"landing_page_url":"http://dx.doi.org/10.1158/1538-7445.am2024-3488","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5109132395","display_name":"Lina Lu","orcid":"https://orcid.org/0000-0003-3138-1478","observed_orcids":["https://orcid.org/0000-0003-3138-1478"]},"institutions":[{"id":"https://openalex.org/I111979921","display_name":"Northwestern University","ror":"https://ror.org/000e0be47","country_code":"US","type":"education","lineage":["https://openalex.org/I111979921"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lina Lu","raw_affiliation_strings":["1Northwestern Univ. Feinberg School of Medicine, chicago, IL;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Northwestern Univ. Feinberg School of Medicine, chicago, IL;","institution_ids":["https://openalex.org/I111979921"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087667707","display_name":"Cheng-Kai Shiau","orcid":"https://orcid.org/0000-0003-0883-8660","observed_orcids":["https://orcid.org/0000-0003-0883-8660"]},"institutions":[{"id":"https://openalex.org/I111979921","display_name":"Northwestern University","ror":"https://ror.org/000e0be47","country_code":"US","type":"education","lineage":["https://openalex.org/I111979921"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Cheng-Kai Shiau","raw_affiliation_strings":["1Northwestern Univ. Feinberg School of Medicine, chicago, IL;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Northwestern Univ. Feinberg School of Medicine, chicago, IL;","institution_ids":["https://openalex.org/I111979921"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051997653","display_name":"Kazutaka Fukumura","orcid":"https://orcid.org/0000-0001-5975-4120","observed_orcids":["https://orcid.org/0000-0001-5975-4120"]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kazutaka Fukumura","raw_affiliation_strings":["2The University of Texas MD Anderson Cancer Center, houston, TX"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2The University of Texas MD Anderson Cancer Center, houston, TX","institution_ids":["https://openalex.org/I1343551460"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101555720","display_name":"Hsiao\u2010Yun Lin","orcid":"https://orcid.org/0000-0002-8295-4339","observed_orcids":["https://orcid.org/0000-0002-8295-4339"]},"institutions":[{"id":"https://openalex.org/I111979921","display_name":"Northwestern University","ror":"https://ror.org/000e0be47","country_code":"US","type":"education","lineage":["https://openalex.org/I111979921"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hsiaoyun Lin","raw_affiliation_strings":["1Northwestern Univ. Feinberg School of Medicine, chicago, IL;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Northwestern Univ. Feinberg School of Medicine, chicago, IL;","institution_ids":["https://openalex.org/I111979921"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5031511560","display_name":"Yueying He","orcid":"https://orcid.org/0000-0003-2082-3656","observed_orcids":["https://orcid.org/0000-0003-2082-3656"]},"institutions":[{"id":"https://openalex.org/I111979921","display_name":"Northwestern University","ror":"https://ror.org/000e0be47","country_code":"US","type":"education","lineage":["https://openalex.org/I111979921"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yueying He","raw_affiliation_strings":["1Northwestern Univ. Feinberg School of Medicine, chicago, IL;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Northwestern Univ. Feinberg School of Medicine, chicago, IL;","institution_ids":["https://openalex.org/I111979921"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065672694","display_name":"Timothy Pan","orcid":"https://orcid.org/0009-0006-4328-1174","observed_orcids":["https://orcid.org/0009-0006-4328-1174"]},"institutions":[{"id":"https://openalex.org/I111979921","display_name":"Northwestern University","ror":"https://ror.org/000e0be47","country_code":"US","type":"education","lineage":["https://openalex.org/I111979921"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Timothy Pan","raw_affiliation_strings":["1Northwestern Univ. Feinberg School of Medicine, chicago, IL;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Northwestern Univ. Feinberg School of Medicine, chicago, IL;","institution_ids":["https://openalex.org/I111979921"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5021372000","display_name":"Lisa M. Norberg","orcid":"https://orcid.org/0009-0007-5741-8773","observed_orcids":["https://orcid.org/0009-0007-5741-8773"]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Lisa Norberg","raw_affiliation_strings":["2The University of Texas MD Anderson Cancer Center, houston, TX"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2The University of Texas MD Anderson Cancer Center, houston, TX","institution_ids":["https://openalex.org/I1343551460"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5038697888","display_name":"Jennifer Ritchie","orcid":"https://orcid.org/0000-0003-2092-1387","observed_orcids":["https://orcid.org/0000-0003-2092-1387"]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jennifer Ritchie","raw_affiliation_strings":["2The University of Texas MD Anderson Cancer Center, houston, TX"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2The University of Texas MD Anderson Cancer Center, houston, TX","institution_ids":["https://openalex.org/I1343551460"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5024476694","display_name":"Jason T. Huse","orcid":"https://orcid.org/0000-0003-4514-0640","observed_orcids":["https://orcid.org/0000-0003-4514-0640"]},"institutions":[{"id":"https://openalex.org/I1343551460","display_name":"The University of Texas MD Anderson Cancer Center","ror":"https://ror.org/04twxam07","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1343551460"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jason T. Huse","raw_affiliation_strings":["2The University of Texas MD Anderson Cancer Center, houston, TX"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"2The University of Texas MD Anderson Cancer Center, houston, TX","institution_ids":["https://openalex.org/I1343551460"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5077837608","display_name":"Ruli Gao","orcid":"https://orcid.org/0000-0002-1137-4564","observed_orcids":["https://orcid.org/0000-0002-1137-4564"]},"institutions":[{"id":"https://openalex.org/I111979921","display_name":"Northwestern University","ror":"https://ror.org/000e0be47","country_code":"US","type":"education","lineage":["https://openalex.org/I111979921"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ruli Gao","raw_affiliation_strings":["1Northwestern Univ. Feinberg School of Medicine, chicago, IL;"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"1Northwestern Univ. Feinberg School of Medicine, chicago, IL;","institution_ids":["https://openalex.org/I111979921"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"84","issue":"6_Supplement","first_page":"3488","last_page":"3488"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.6298999786376953,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10604","display_name":"RNA Research and Splicing","score":0.6298999786376953,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11289","display_name":"Single-cell and spatial transcriptomics","score":0.19259999692440033,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10515","display_name":"Cancer-related molecular mechanisms research","score":0.0544000007212162,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/melanoma-brain-metastases","display_name":"melanoma brain metastases","score":0.9240000247955322},{"id":"https://openalex.org/keywords/alternative-splicing","display_name":"alternative splicing","score":0.9909999966621399},{"id":"https://openalex.org/keywords/splicing-isoforms","display_name":"splicing isoforms","score":0.9490000009536743},{"id":"https://openalex.org/keywords/extracranial-metastasis","display_name":"extracranial metastases","score":0.8379999995231628},{"id":"https://openalex.org/keywords/glucose-metabolism","display_name":"glucose metabolism","score":0.49000000953674316},{"id":"https://openalex.org/keywords/lipid-metabolism","display_name":"lipid metabolism","score":1.0},{"id":"https://openalex.org/keywords/spliceosome","display_name":"spliceosome","score":0.7739999890327454},{"id":"https://openalex.org/keywords/tumor-microenvironment","display_name":"tumor microenvironment","score":0.656000018119812}],"concepts":[{"id":"https://openalex.org/C54458228","wikidata":"https://www.wikidata.org/wiki/Q237218","display_name":"RNA splicing","level":4,"score":0.6799941658973694},{"id":"https://openalex.org/C194583182","wikidata":"https://www.wikidata.org/wiki/Q21114084","display_name":"Alternative splicing","level":4,"score":0.579413652420044},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5676580667495728},{"id":"https://openalex.org/C53345823","wikidata":"https://www.wikidata.org/wiki/Q5531554","display_name":"Gene isoform","level":3,"score":0.5147174000740051},{"id":"https://openalex.org/C57273362","wikidata":"https://www.wikidata.org/wiki/Q576722","display_name":"Decoding methods","level":2,"score":0.5036928057670593},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.490043044090271},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4742499887943268},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.36041033267974854},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3194260895252228},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.13758200407028198},{"id":"https://openalex.org/C11413529","wikidata":"https://www.wikidata.org/wiki/Q8366","display_name":"Algorithm","level":1,"score":0.1373593807220459}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1158/1538-7445.am2024-3488","doi":"https://doi.org/10.1158/1538-7445.am2024-3488","is_oa":false,"landing_page_url":"http://dx.doi.org/10.1158/1538-7445.am2024-3488","pdf_url":null,"source":{"id":"https://openalex.org/S168522863","display_name":"Cancer Research","issn_l":"0008-5472","issn":["0008-5472","1538-7445"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320273","host_organization_name":"American Association for Cancer Research","host_organization_lineage":["https://openalex.org/P4310320273"],"host_organization_lineage_names":["American Association for Cancer Research"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Cancer Research","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8371999859809875,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7039133488","https://openalex.org/W7155376835","https://openalex.org/W4416582158","https://openalex.org/W4402050312","https://openalex.org/W4282971794","https://openalex.org/W4205895577","https://openalex.org/W4396516138","https://openalex.org/W2939217416","https://openalex.org/W7148778600","https://openalex.org/W4379878423"],"abstract_inverted_index":{"Abstract":[0],"Melanoma":[1],"brain":[2,143,274],"metastasis":[3,12,98],"(MBM)":[4],"is":[5],"the":[6,14,59,104,216,225,286],"third":[7],"most":[8],"common":[9],"origin":[10],"of":[11,21,62,76,151,156,172,192,218,228,285],"to":[13,50,73,102,106,131,201],"brain,":[15],"with":[16,196],"a":[17,27],"median":[18],"overall":[19],"survival":[20],"8.9":[22],"months.":[23],"Alternative":[24],"splicing":[25,108,173,185,221,241,267],"plays":[26],"critical":[28],"role":[29],"in":[30,38,47,69,114,142,160,209,224,272],"cancer":[31],"pathogenesis":[32],"through":[33,276],"altered":[34],"protein":[35],"coding":[36],"complexity":[37],"tumor":[39,100,137,197,237],"cells,":[40],"which":[41,107],"however":[42],"has":[43,58],"been":[44],"poorly":[45],"studied":[46],"MBM":[48,95,120],"due":[49,72],"technical":[51],"limitations.":[52],"Long-read":[53],"single":[54,87,277],"cell":[55,88,198,278],"RNA":[56,90,280],"sequencing":[57,91,281],"unique":[60],"strength":[61],"measuring":[63,77],"transcript":[64],"isoforms":[65,109,157,268],"and":[66,96,126,162,184,220,239,269],"mutations":[67],"simultaneously":[68],"individual":[70],"cells":[71],"its":[74],"capacity":[75],"full-length":[78],"transcripts.":[79],"In":[80],"this":[81],"study,":[82],"we":[83,188],"employed":[84],"high":[85],"throughout":[86],"long-read":[89,279],"(scNanoRNAseq)":[92],"onto":[93],"patient-matched":[94],"extracranial":[97],"(ECM)":[99],"pairs":[101],"evaluate":[103],"extent":[105],"impact":[110,204],"adaptive":[111,206,226,270],"clonal":[112,207],"evolution":[113,227,271],"MBM.":[115,210],"Our":[116],"results":[117],"showed":[118],"that":[119,136,149,170],"tumors":[121],"had":[122],"distinct":[123],"genetic":[124],"alterations":[125],"ecological":[127],"cellular":[128],"compositions":[129],"compared":[130],"matched":[132],"ECM":[133],"tumors,":[134],"indicating":[135],"clones":[138],"were":[139,177],"adaptively":[140],"expanded":[141],"microenvironment.":[144],"Comparative":[145],"isoform":[146,193],"analysis":[147],"revealed":[148],"hundreds":[150],"genes":[152,175],"expressed":[153],"different":[154],"combinations":[155],"(DCIs)":[158],"involved":[159],"glucose":[161],"lipid":[163],"metabolic":[164],"pathways.":[165],"Interestingly,":[166],"our":[167,213],"data":[168],"demonstrated":[169],"~10%":[171],"related":[174],"themselves":[176],"alternatively":[178],"spliced":[179],"including":[180],"both":[181],"spliceosome":[182],"members":[183],"activators.":[186],"Furthermore,":[187],"calculated":[189],"temporal":[190],"changes":[191],"usages":[194],"along":[195],"evolutionary":[199],"lineages":[200],"quantify":[202],"their":[203],"on":[205],"expansion":[208],"Taken":[211],"together,":[212],"work":[214],"improves":[215],"understanding":[217],"transcriptional":[219],"dynamics":[222],"inherent":[223],"MBM,":[229],"providing":[230],"evidence":[231],"for":[232,289],"developing":[233],"new":[234],"therapeutics":[235],"targeting":[236],"cell-specific":[238],"MBM-specific":[240],"isoforms.":[242],"Citation":[243],"Format:":[244],"Lina":[245],"Lu,":[246],"Cheng-Kai":[247],"Shiau,":[248],"Kazutaka":[249],"Fukumura,":[250],"Hsiaoyun":[251],"Lin,":[252],"Yueying":[253],"He,":[254],"Timothy":[255],"Pan,":[256],"Lisa":[257],"Norberg,":[258],"Jennifer":[259],"Ritchie,":[260],"Jason":[261],"T.":[262],"Huse,":[263],"Ruli":[264],"Gao.":[265],"Decoding":[266],"melanoma":[273],"metastases":[275],"[abstract].":[282],"In:":[283],"Proceedings":[284],"American":[287],"Association":[288],"Cancer":[290,308],"Research":[291],"Annual":[292],"Meeting":[293],"2024;":[294],"Part":[295],"1":[296],"(Regular":[297],"Abstracts);":[298],"2024":[299],"Apr":[300],"5-10;":[301],"San":[302],"Diego,":[303],"CA.":[304],"Philadelphia":[305],"(PA):":[306],"AACR;":[307],"Res":[309],"2024;84(6_Suppl):Abstract":[310],"nr":[311],"3488.":[312]},"counts_by_year":[],"updated_date":"2026-09-22T06:50:58.402377","created_date":"2024-03-23T00:00:00"}