{"id":"https://openalex.org/W4392887366","doi":"https://doi.org/10.1227/neu.0000000000002809_305","title":"305 Extracellular Vesicular mRNA and miRNA Biomarkers in Patients With Glioblastoma","display_name":"305 Extracellular Vesicular mRNA and miRNA Biomarkers in Patients With Glioblastoma","publication_year":2024,"publication_date":"2024-03-15","ids":{"openalex":"https://openalex.org/W4392887366","doi":"https://doi.org/10.1227/neu.0000000000002809_305"},"language":"en","primary_location":{"id":"doi:10.1227/neu.0000000000002809_305","doi":"https://doi.org/10.1227/neu.0000000000002809_305","is_oa":false,"landing_page_url":"https://doi.org/10.1227/neu.0000000000002809_305","pdf_url":null,"source":{"id":"https://openalex.org/S111727011","display_name":"Neurosurgery","issn_l":"0148-396X","issn":["0148-396X","1081-1281","1524-4040"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Neurosurgery","raw_type":"journal-article"},"volume":null,"type":"conference-abstract","indexed_in":["crossref"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5087286844","display_name":"Kristin Michelle Huntoon","orcid":"https://orcid.org/0000-0002-7211-8986","observed_orcids":["https://orcid.org/0000-0002-7211-8986"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Kristin Huntoon","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100339335","display_name":"Hong Li","orcid":"https://orcid.org/0000-0001-5679-9980","observed_orcids":["https://orcid.org/0000-0001-5679-9980"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Li Hong","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5094174136","display_name":"Jingjing Zhnag","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jingjing Zhnag","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5017997501","display_name":"Betty Y.S. Kim","orcid":"https://orcid.org/0000-0001-6890-8355","observed_orcids":["https://orcid.org/0000-0001-6890-8355"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Betty Y.S. Kim","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090210760","display_name":"Wen Jiang","orcid":"https://orcid.org/0000-0001-9154-633X","observed_orcids":["https://orcid.org/0000-0001-9154-633X","https://orcid.org/0000-0003-0612-2917","https://orcid.org/0000-0002-6073-3260","https://orcid.org/0000-0001-6858-2666"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Wen Jiang","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":null,"display_name":"James Lee","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"James Lee","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5089531881","display_name":"Eduardo Re\u00e1tegui","orcid":"https://orcid.org/0000-0002-8271-8205","observed_orcids":["https://orcid.org/0000-0002-8271-8205"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Eduardo Reategui","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"70","issue":"Supplement_1","first_page":"88","last_page":"88"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10773","display_name":"Extracellular vesicles in disease","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10773","display_name":"Extracellular vesicles in disease","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10062","display_name":"MicroRNA in disease regulation","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1306","display_name":"Cancer Research"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10129","display_name":"Glioma Diagnosis and Treatment","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics (clinical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glioblastoma","display_name":"glioblastoma","score":0.9990000128746033},{"id":"https://openalex.org/keywords/extracellular-vesicles","display_name":"extracellular vesicles","score":1.0},{"id":"https://openalex.org/keywords/serum-biomarkers","display_name":"serum biomarkers","score":0.7059999704360962},{"id":"https://openalex.org/keywords/total-internal-reflection-fluorescence-microscopy","display_name":"total internal reflection fluorescence microscopy","score":0.7940000295639038},{"id":"https://openalex.org/keywords/crossflow-filtration","display_name":"cross-flow filtration","score":0.7929999828338623},{"id":"https://openalex.org/keywords/non-invasive-diagnosis","display_name":"non-invasive diagnosis","score":0.6800000071525574}],"concepts":[{"id":"https://openalex.org/C145059251","wikidata":"https://www.wikidata.org/wiki/Q310899","display_name":"microRNA","level":3,"score":0.7679897546768188},{"id":"https://openalex.org/C2781197716","wikidata":"https://www.wikidata.org/wiki/Q864574","display_name":"Biomarker","level":2,"score":0.6098001003265381},{"id":"https://openalex.org/C2992929900","wikidata":"https://www.wikidata.org/wiki/Q21097485","display_name":"Extracellular vesicles","level":2,"score":0.607235848903656},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.5605239868164062},{"id":"https://openalex.org/C87892846","wikidata":"https://www.wikidata.org/wiki/Q864256","display_name":"Biochip","level":2,"score":0.5092734098434448},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.4946279227733612},{"id":"https://openalex.org/C2908689518","wikidata":"https://www.wikidata.org/wiki/Q21097485","display_name":"Extracellular vesicle","level":5,"score":0.4649238586425781},{"id":"https://openalex.org/C121608353","wikidata":"https://www.wikidata.org/wiki/Q12078","display_name":"Cancer","level":2,"score":0.42566633224487305},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.41908717155456543},{"id":"https://openalex.org/C502942594","wikidata":"https://www.wikidata.org/wiki/Q3421914","display_name":"Cancer research","level":1,"score":0.4169881343841553},{"id":"https://openalex.org/C20518536","wikidata":"https://www.wikidata.org/wiki/Q6840469","display_name":"Microvesicles","level":4,"score":0.3849494159221649},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.23462170362472534},{"id":"https://openalex.org/C60644358","wikidata":"https://www.wikidata.org/wiki/Q128570","display_name":"Bioinformatics","level":1,"score":0.23028290271759033},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.20224681496620178},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.14219734072685242},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.11319577693939209},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1058204174041748}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1227/neu.0000000000002809_305","doi":"https://doi.org/10.1227/neu.0000000000002809_305","is_oa":false,"landing_page_url":"https://doi.org/10.1227/neu.0000000000002809_305","pdf_url":null,"source":{"id":"https://openalex.org/S111727011","display_name":"Neurosurgery","issn_l":"0148-396X","issn":["0148-396X","1081-1281","1524-4040"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310315671","host_organization_name":"Lippincott Williams & Wilkins","host_organization_lineage":["https://openalex.org/P4310315671","https://openalex.org/P4310318547"],"host_organization_lineage_names":["Lippincott Williams & Wilkins","Wolters Kluwer"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Neurosurgery","raw_type":"journal-article"}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3","score":0.9537000060081482}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W3180499128","https://openalex.org/W7154651397","https://openalex.org/W3103756951","https://openalex.org/W3041135638","https://openalex.org/W4416141178","https://openalex.org/W7017734159","https://openalex.org/W4403510780","https://openalex.org/W4416916030","https://openalex.org/W2150309717","https://openalex.org/W2122676495"],"abstract_inverted_index":{"INTRODUCTION:":[0],"Glioblastoma":[1],"(GBM)":[2],"is":[3,11,19],"a":[4,20,35,117,124,128,214,233],"highly":[5],"lethal":[6],"primary":[7],"brain":[8],"cancer":[9,85],"that":[10],"resistant":[12],"to":[13,104,232],"conventional":[14],"immunotherapies.":[15],"However,":[16],"currently,":[17],"there":[18],"lack":[21],"of":[22,64,171,187,200,207,224,226,235,266],"reliable":[23],"biomarkers":[24,163,189],"for":[25,38,164,195,256],"clinical":[26],"practice.":[27],"Extracellular":[28],"vesicles":[29],"(EVs)":[30],"present":[31],"in":[32,112,192,205,264],"biofluids":[33],"are":[34,100],"potential":[36,161,272],"biomarker":[37],"GBM":[39,50,78,172,273],"diagnosis.":[40],"METHODS:":[41],"EVs":[42,65,73,113,167,267],"from":[43,74,168,237],"cell":[44],"culture":[45],"medium,":[46],"healthy":[47,75,238],"donor":[48,76],"and":[49,62,70,77,91,97,109,147,150,155,184,241,262],"patient":[51,79],"serum":[52,80,170,236],"were":[53,66,81,114,133,158,177,190,230],"purified":[54],"using":[55,88,123,135],"tangential":[56],"flow":[57],"filtration":[58],"(TFF).":[59],"Morphology,":[60],"size,":[61],"numbers":[63],"characterized":[67],"by":[68,116],"TEM":[69],"qNano,":[71],"respectively.":[72],"compared":[82,231],"against":[83],"other":[84],"EV":[86,257],"samples":[87],"bulk":[89,145],"mRNA":[90,96,108,146,157,183],"miRNA":[92,98,110,148,154,185],"sequencing":[93,149],"analysis.":[94],"Selected":[95,260],"name":[99],"not":[101],"mentioned":[102],"due":[103],"patents":[105],"being":[106],"drafted.":[107],"expression":[111,186,225],"quantified":[115],"fluorescent":[118],"nucleic":[119],"acid":[120],"detection":[121],"method":[122,217],"biochip":[125,181,252],"platform":[126,210],"at":[127],"single-EV":[129],"level.":[130],"Fluorescence":[131],"images":[132],"recorded":[134],"high-resolution":[136],"total":[137],"internal":[138],"reflection":[139],"fluorescence":[140],"(TIRF)":[141],"microscopy.":[142],"RESULTS:":[143],"From":[144],"bioinformatics":[151],"analysis,":[152],"three":[153],"two":[156,247],"selected":[159],"as":[160,271],"candidate":[162],"GBM.":[165],"Subsequently,":[166],"the":[169,227,246],"patients":[173],"(n":[174],"=":[175],"39)":[176],"evaluated":[178],"through":[179],"our":[180],"platform.":[182],"these":[188],"positive":[191],"single":[193],"EVs,":[194],"which":[196],"we":[197],"detected":[198],"colocalization":[199],"protein/RNAs":[201],"or":[202],"multiple":[203],"RNAs":[204,229],"subpopulations":[206,265],"EVs.":[208],"Our":[209,250],"was":[211],"benchmarked":[212],"with":[213],"standard":[215],"PCR":[216],"showing":[218,242],"100X":[219],"more":[220],"sensitivity.":[221],"Moreover,":[222],"levels":[223],"different":[228],"cohort":[234],"donors":[239],"(n=20),":[240],"significant":[243],"differences":[244],"between":[245],"groups.":[248],"CONCLUSIONS:":[249],"non-invasive":[251],"assay":[253],"outperformed":[254],"qRT-PCR":[255],"RNA":[258],"detection.":[259],"mRNAs":[261],"miRNAs":[263],"could":[268],"be":[269],"used":[270],"biomarkers.":[274]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2024-03-17T00:00:00"}