{"id":"https://openalex.org/W4391848851","doi":"https://doi.org/10.1016/j.jsb.2024.108068","title":"A strategic approach for efficient cryo-EM grid optimization using design of experiments","display_name":"A strategic approach for efficient cryo-EM grid optimization using design of experiments","publication_year":2024,"publication_date":"2024-02-15","ids":{"openalex":"https://openalex.org/W4391848851","doi":"https://doi.org/10.1016/j.jsb.2024.108068","pmid":"https://pubmed.ncbi.nlm.nih.gov/38364988","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/12327211"},"language":"en","primary_location":{"id":"doi:10.1016/j.jsb.2024.108068","doi":"https://doi.org/10.1016/j.jsb.2024.108068","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.jsb.2024.108068","pdf_url":null,"source":{"id":"https://openalex.org/S89954039","display_name":"Journal of Structural Biology","issn_l":"1047-8477","issn":["1047-8477","1095-8657"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Structural Biology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/j.jsb.2024.108068","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5077117756","display_name":"Rose Marie Haynes","orcid":"https://orcid.org/0000-0002-7382-3169","observed_orcids":["https://orcid.org/0000-0002-7382-3169"]},"institutions":[{"id":"https://openalex.org/I142606810","display_name":"Pacific Northwest National Laboratory","ror":"https://ror.org/05h992307","country_code":"US","type":"facility","lineage":["https://openalex.org/I1325736334","https://openalex.org/I1330989302","https://openalex.org/I142606810","https://openalex.org/I39565521"]},{"id":"https://openalex.org/I165690674","display_name":"Oregon Health & Science University","ror":"https://ror.org/009avj582","country_code":"US","type":"education","lineage":["https://openalex.org/I165690674"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Rose Marie Haynes","raw_affiliation_strings":["Pacific Northwest Center for Cryo-Electron Microscopy, Oregon Health & Science University, Portland, OR 97201, USA; Pacific Northwest National Laboratory, Richland, WA 99354, USA. Electronic address: rose.haynes@pnnl.gov","Pacific Northwest Center for Cryo-Electron Microscopy, Oregon Health & Science University, Portland, OR 97201, USA","Pacific Northwest National Laboratory, Richland, WA 99354, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pacific Northwest Center for Cryo-Electron Microscopy, Oregon Health & Science University, Portland, OR 97201, USA; Pacific Northwest National Laboratory, Richland, WA 99354, USA. Electronic address: rose.haynes@pnnl.gov","institution_ids":["https://openalex.org/I142606810","https://openalex.org/I165690674"]},{"raw_affiliation_string":"Pacific Northwest Center for Cryo-Electron Microscopy, Oregon Health & Science University, Portland, OR 97201, USA","institution_ids":["https://openalex.org/I165690674"]},{"raw_affiliation_string":"Pacific Northwest National Laboratory, Richland, WA 99354, USA","institution_ids":["https://openalex.org/I142606810"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053345669","display_name":"Janette B. Myers","orcid":"https://orcid.org/0000-0002-7758-2649","observed_orcids":["https://orcid.org/0000-0002-7758-2649"]},"institutions":[{"id":"https://openalex.org/I165690674","display_name":"Oregon Health & Science University","ror":"https://ror.org/009avj582","country_code":"US","type":"education","lineage":["https://openalex.org/I165690674"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Janette Myers","raw_affiliation_strings":["Pacific Northwest Center for Cryo-Electron Microscopy, Oregon Health & Science University, Portland, OR 97201, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pacific Northwest Center for Cryo-Electron Microscopy, Oregon Health & Science University, Portland, OR 97201, USA","institution_ids":["https://openalex.org/I165690674"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5150009123","display_name":"Claudia S. L\u00f3pez","orcid":"https://orcid.org/0000-0003-4668-3524","observed_orcids":["https://orcid.org/0000-0003-4668-3524"]},"institutions":[{"id":"https://openalex.org/I165690674","display_name":"Oregon Health & Science University","ror":"https://ror.org/009avj582","country_code":"US","type":"education","lineage":["https://openalex.org/I165690674"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Claudia S. L\u00f3pez","raw_affiliation_strings":["Pacific Northwest Center for Cryo-Electron Microscopy, Oregon Health & Science University, Portland, OR 97201, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pacific Northwest Center for Cryo-Electron Microscopy, Oregon Health & Science University, Portland, OR 97201, USA","institution_ids":["https://openalex.org/I165690674"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5132564885","display_name":"James E. Evans","orcid":"https://orcid.org/0000-0002-7721-4782","observed_orcids":["https://orcid.org/0000-0002-7721-4782"]},"institutions":[{"id":"https://openalex.org/I142606810","display_name":"Pacific Northwest National Laboratory","ror":"https://ror.org/05h992307","country_code":"US","type":"facility","lineage":["https://openalex.org/I1325736334","https://openalex.org/I1330989302","https://openalex.org/I142606810","https://openalex.org/I39565521"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James Evans","raw_affiliation_strings":["Pacific Northwest National Laboratory, Richland, WA 99354, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pacific Northwest National Laboratory, Richland, WA 99354, USA","institution_ids":["https://openalex.org/I142606810"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088940545","display_name":"Omar Davulcu","orcid":"https://orcid.org/0000-0002-7056-6435","observed_orcids":["https://orcid.org/0000-0002-7056-6435"]},"institutions":[{"id":"https://openalex.org/I165690674","display_name":"Oregon Health & Science University","ror":"https://ror.org/009avj582","country_code":"US","type":"education","lineage":["https://openalex.org/I165690674"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Omar Davulcu","raw_affiliation_strings":["Pacific Northwest Center for Cryo-Electron Microscopy, Oregon Health & Science University, Portland, OR 97201, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pacific Northwest Center for Cryo-Electron Microscopy, Oregon Health & Science University, Portland, OR 97201, USA","institution_ids":["https://openalex.org/I165690674"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5013854148","display_name":"Craig Yoshioka","orcid":"https://orcid.org/0000-0002-0251-7316","observed_orcids":["https://orcid.org/0000-0002-0251-7316"]},"institutions":[{"id":"https://openalex.org/I165690674","display_name":"Oregon Health & Science University","ror":"https://ror.org/009avj582","country_code":"US","type":"education","lineage":["https://openalex.org/I165690674"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Craig Yoshioka","raw_affiliation_strings":["Pacific Northwest Center for Cryo-Electron Microscopy, Oregon Health & Science University, Portland, OR 97201, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pacific Northwest Center for Cryo-Electron Microscopy, Oregon Health & Science University, Portland, OR 97201, USA","institution_ids":["https://openalex.org/I165690674"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5077117756"],"corresponding_institution_ids":["https://openalex.org/I142606810","https://openalex.org/I165690674"],"apc_list":{"value":4220,"currency":"USD","value_usd":4220},"apc_paid":{"value":4220,"currency":"USD","value_usd":4220},"fwci":0.7884,"has_fulltext":true,"cited_by_count":5,"citation_normalized_percentile":{"value":0.68719965,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":97},"biblio":{"volume":"217","issue":"1","first_page":"108068","last_page":"108068"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10857","display_name":"Advanced Electron Microscopy Techniques and Applications","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/vitrification","display_name":"vitrification","score":0.6489999890327454},{"id":"https://openalex.org/keywords/experimental-design","display_name":"experimental design","score":0.7639999985694885},{"id":"https://openalex.org/keywords/fractional-factorial-design","display_name":"fractional factorial design","score":0.9950000047683716},{"id":"https://openalex.org/keywords/ice-thickness","display_name":"ice thickness","score":0.7279999852180481},{"id":"https://openalex.org/keywords/particle-distribution","display_name":"particle distribution","score":0.9850000143051147},{"id":"https://openalex.org/keywords/plunge-freezing","display_name":"plunge freezing","score":0.5630000233650208},{"id":"https://openalex.org/keywords/cryo-em-sample-preparation","display_name":"cryo-EM sample preparation","score":0.7120000123977661},{"id":"https://openalex.org/keywords/apoferritin","display_name":"apoferritin","score":0.7829999923706055},{"id":"https://openalex.org/keywords/l-glutamate-dehydrogenase","display_name":"L-glutamate dehydrogenase","score":0.953000009059906},{"id":"https://openalex.org/keywords/regression-models","display_name":"regression models","score":0.4690000116825104}],"concepts":[{"id":"https://openalex.org/C187691185","wikidata":"https://www.wikidata.org/wiki/Q2020720","display_name":"Grid","level":2,"score":0.6971365213394165},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.6239666938781738},{"id":"https://openalex.org/C2780513914","wikidata":"https://www.wikidata.org/wiki/Q18210350","display_name":"Bottleneck","level":2,"score":0.5602402091026306},{"id":"https://openalex.org/C198531522","wikidata":"https://www.wikidata.org/wiki/Q485146","display_name":"Sample (material)","level":2,"score":0.5097717642784119},{"id":"https://openalex.org/C177212765","wikidata":"https://www.wikidata.org/wiki/Q627335","display_name":"Workflow","level":2,"score":0.501082181930542},{"id":"https://openalex.org/C34559072","wikidata":"https://www.wikidata.org/wiki/Q2334061","display_name":"Design of experiments","level":2,"score":0.485780268907547},{"id":"https://openalex.org/C98045186","wikidata":"https://www.wikidata.org/wiki/Q205663","display_name":"Process (computing)","level":2,"score":0.470839262008667},{"id":"https://openalex.org/C129848803","wikidata":"https://www.wikidata.org/wiki/Q2564360","display_name":"Sample size determination","level":2,"score":0.44404393434524536},{"id":"https://openalex.org/C124101348","wikidata":"https://www.wikidata.org/wiki/Q172491","display_name":"Data mining","level":1,"score":0.39116472005844116},{"id":"https://openalex.org/C126255220","wikidata":"https://www.wikidata.org/wiki/Q141495","display_name":"Mathematical optimization","level":1,"score":0.370033323764801},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.1776050329208374},{"id":"https://openalex.org/C105795698","wikidata":"https://www.wikidata.org/wiki/Q12483","display_name":"Statistics","level":1,"score":0.16514793038368225},{"id":"https://openalex.org/C77088390","wikidata":"https://www.wikidata.org/wiki/Q8513","display_name":"Database","level":1,"score":0.10646820068359375},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.0},{"id":"https://openalex.org/C2524010","wikidata":"https://www.wikidata.org/wiki/Q8087","display_name":"Geometry","level":1,"score":0.0},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.0},{"id":"https://openalex.org/C149635348","wikidata":"https://www.wikidata.org/wiki/Q193040","display_name":"Embedded system","level":1,"score":0.0},{"id":"https://openalex.org/C111919701","wikidata":"https://www.wikidata.org/wiki/Q9135","display_name":"Operating system","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D001052","descriptor_name":"Apoferritins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013048","descriptor_name":"Specimen Handling","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D020285","descriptor_name":"Cryoelectron Microscopy","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":true},{"descriptor_ui":"D020285","descriptor_name":"Cryoelectron Microscopy","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D058989","descriptor_name":"Vitrification","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":6,"locations":[{"id":"doi:10.1016/j.jsb.2024.108068","doi":"https://doi.org/10.1016/j.jsb.2024.108068","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.jsb.2024.108068","pdf_url":null,"source":{"id":"https://openalex.org/S89954039","display_name":"Journal of Structural Biology","issn_l":"1047-8477","issn":["1047-8477","1095-8657"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Structural Biology","raw_type":"journal-article"},{"id":"doi:10.2139/ssrn.4647448","doi":"https://doi.org/10.2139/ssrn.4647448","is_oa":true,"landing_page_url":"https://doi.org/10.2139/ssrn.4647448","pdf_url":null,"source":{"id":"https://openalex.org/S4210172589","display_name":"SSRN Electronic Journal","issn_l":"1556-5068","issn":["1556-5068"],"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1318003438","host_organization_name":"RELX Group (Netherlands)","host_organization_lineage":["https://openalex.org/I1318003438"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"posted-content"},{"id":"pmid:38364988","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/38364988","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of structural biology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:12327211","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/12327211","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC12327211/pdf/nihms-2099330.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"J Struct Biol","raw_type":"Text"},{"id":"pmh:oai:osti.gov:2327069","doi":null,"is_oa":false,"landing_page_url":"https://www.osti.gov/biblio/2327069","pdf_url":null,"source":{"id":"https://openalex.org/S4306402487","display_name":"OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I139351228","host_organization_name":"Office of Scientific and Technical Information","host_organization_lineage":["https://openalex.org/I139351228"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null},{"id":"pmh:oai:osti.gov:2564668","doi":null,"is_oa":false,"landing_page_url":"https://www.osti.gov/biblio/2564668","pdf_url":null,"source":{"id":"https://openalex.org/S4306402487","display_name":"OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I139351228","host_organization_name":"Office of Scientific and Technical Information","host_organization_lineage":["https://openalex.org/I139351228"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"doi:10.1016/j.jsb.2024.108068","doi":"https://doi.org/10.1016/j.jsb.2024.108068","is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.jsb.2024.108068","pdf_url":null,"source":{"id":"https://openalex.org/S89954039","display_name":"Journal of Structural Biology","issn_l":"1047-8477","issn":["1047-8477","1095-8657"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Structural Biology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G5868986941","display_name":"Pacific Northwest Center for Cryo-EM","funder_award_id":"U24GM129547","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320306084","display_name":"U.S. Department of Energy","ror":"https://ror.org/01bj3aw27"},{"id":"https://openalex.org/F4320309233","display_name":"Oregon Health and Science University","ror":"https://ror.org/009avj582"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320332359","display_name":"Office of Science","ror":"https://ror.org/00mmn6b08"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337509","display_name":"Biological and Environmental Research","ror":"https://ror.org/0114b2m14"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4391848851.pdf","grobid_xml":"https://content.openalex.org/works/W4391848851.grobid-xml"},"referenced_works_count":35,"referenced_works":["https://openalex.org/W1528036327","https://openalex.org/W1883464583","https://openalex.org/W1989921222","https://openalex.org/W2034051496","https://openalex.org/W2041956358","https://openalex.org/W2094246236","https://openalex.org/W2140444174","https://openalex.org/W2142161929","https://openalex.org/W2178041119","https://openalex.org/W2212589734","https://openalex.org/W2253702229","https://openalex.org/W2409722811","https://openalex.org/W2496831902","https://openalex.org/W2508012814","https://openalex.org/W2587625522","https://openalex.org/W2777092774","https://openalex.org/W2801098531","https://openalex.org/W2804636455","https://openalex.org/W3006716706","https://openalex.org/W3027534552","https://openalex.org/W3094051077","https://openalex.org/W3097900553","https://openalex.org/W3155606786","https://openalex.org/W3160504190","https://openalex.org/W3179344552","https://openalex.org/W3185665678","https://openalex.org/W4200214531","https://openalex.org/W4200311841","https://openalex.org/W4285730949","https://openalex.org/W4291368660","https://openalex.org/W4312083688","https://openalex.org/W4319293815","https://openalex.org/W4387266165","https://openalex.org/W4388715001","https://openalex.org/W6774242788"],"related_works":["https://openalex.org/W4319880042","https://openalex.org/W4412910260","https://openalex.org/W4286470598","https://openalex.org/W3047162335","https://openalex.org/W4391044987","https://openalex.org/W2976378015","https://openalex.org/W3192148604","https://openalex.org/W2804636455","https://openalex.org/W4389280548","https://openalex.org/W4406090291"],"abstract_inverted_index":{"In":[0],"recent":[1],"years,":[2],"cryo-electron":[3],"microscopy":[4],"(cryo-EM)":[5],"has":[6],"become":[7],"a":[8,33,78,90,114,119,171,212,224,291,296,300],"practical":[9],"and":[10,26,57,71,122,156,192,197,217,254,263,279,302],"effective":[11],"method":[12],"of":[13,45,54,105,116,149,151,170,174,184,206,228],"determining":[14],"structures":[15],"at":[16],"previously":[17],"unattainable":[18],"resolutions":[19],"due":[20],"to":[21,59,128,154,204,222,237],"advances":[22],"in":[23,36,138,232,243],"detection,":[24],"automation,":[25],"data":[27,75],"processing.":[28],"However,":[29],"sample":[30,47,80],"preparation":[31],"remains":[32],"major":[34],"bottleneck":[35],"the":[37,42,61,131,158,168,180,229,239,259,264,269],"cryo-EM":[38,98,306],"workflow.":[39],"Even":[40],"after":[41],"arduous":[43],"process":[44,108,134],"biochemical":[46],"optimization,":[48],"it":[49],"often":[50],"takes":[51],"several":[52],"iterations":[53,141],"grid":[55,63,87,101,132,159,208,271,307],"vitrification":[56],"screening":[58],"determine":[60,238],"optimal":[62,246],"freezing":[64],"parameters":[65],"that":[66,130,282,295],"yield":[67,245],"suitable":[68,284],"ice":[69,198,278],"thickness":[70],"particle":[72,195,280],"distribution":[73,196,281],"for":[74,83,100,194,285,305],"collection.":[76],"Since":[77],"high-quality":[79,277],"is":[81,89,235,299],"imperative":[82],"high-resolution":[84],"structure":[85],"determination,":[86],"optimization":[88,107,133,160],"vital":[91],"step.":[92],"For":[93],"researchers":[94],"who":[95],"rely":[96],"on":[97,211,252,257,290],"facilities":[99],"screening,":[102],"each":[103,205],"iteration":[104],"this":[106,250],"may":[109],"delay":[110],"research":[111],"progress":[112],"by":[113],"matter":[115],"months.":[117],"Therefore,":[118],"more":[120],"strategic":[121],"efficient":[123],"approach":[124,251,298],"should":[125],"be":[126,136],"taken":[127],"ensure":[129],"can":[135,178],"completed":[137],"as":[139,142],"few":[140],"possible.":[143],"Here,":[144],"we":[145],"present":[146],"an":[147],"implementation":[148],"Design":[150,165],"Experiments":[152],"(DOE)":[153],"expedite":[155],"strategize":[157],"process.":[161],"A":[162],"Fractional":[163],"Factorial":[164],"(FFD)":[166],"guides":[167],"determination":[169],"limited":[172],"set":[173],"experimental":[175],"conditions":[176,191,216,240,272],"which":[177,234,241],"model":[179,227],"full":[181],"parameter":[182,230],"space":[183,231],"interest.":[185],"Grids":[186],"are":[187,202,220],"frozen":[188],"with":[189,276],"these":[190,207],"screened":[193],"thickness.":[199],"Quantitative":[200],"scores":[201,219],"assigned":[203],"characteristics":[209],"based":[210],"qualitative":[213],"rubric.":[214],"Input":[215],"response":[218],"used":[221,236],"generate":[223],"least-squares":[225],"regression":[226],"JMP,":[233],"should,":[242],"theory,":[244],"grids.":[247],"Upon":[248],"testing":[249],"apoferritin":[253],"L-glutamate":[255],"dehydrogenase":[256],"both":[258],"Vitrobot":[260],"Mark":[261],"IV":[262],"Leica":[265],"GP2":[266],"plunge":[267],"freezers,":[268],"resulting":[270],"reliably":[273],"yielded":[274],"grids":[275],"were":[283],"collecting":[286],"large":[287],"overnight":[288],"datasets":[289],"Krios.":[292],"We":[293],"conclude":[294],"DOE-based":[297],"cost-effective":[301],"time-saving":[303],"tool":[304],"preparation.":[308]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":2}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2024-02-16T00:00:00"}