{"id":"https://openalex.org/W4391406738","doi":"https://doi.org/10.1089/regen.2023.0026","title":"Direct Plasmid DNA Transfection into Differentiated Mouse C2C12 Myotubes","display_name":"Direct Plasmid DNA Transfection into Differentiated Mouse C2C12 Myotubes","publication_year":2024,"publication_date":"2024-01-01","ids":{"openalex":"https://openalex.org/W4391406738","doi":"https://doi.org/10.1089/regen.2023.0026"},"language":"en","primary_location":{"id":"doi:10.1089/regen.2023.0026","doi":"https://doi.org/10.1089/regen.2023.0026","is_oa":true,"landing_page_url":"http://dx.doi.org/10.1089/regen.2023.0026","pdf_url":null,"source":{"id":"https://openalex.org/S4210214794","display_name":"Re GEN Open","issn_l":"2766-2705","issn":["2766-2705"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Re:GEN Open","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://dx.doi.org/10.1089/regen.2023.0026","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5010262841","display_name":"Amanda Sales Conniff","orcid":"https://orcid.org/0000-0002-6151-1897","observed_orcids":["https://orcid.org/0000-0002-6151-1897"]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Amanda Sales Conniff","raw_affiliation_strings":["Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida","Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida, Tampa, FL 33612, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida","institution_ids":["https://openalex.org/I2613432"]},{"raw_affiliation_string":"Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida, Tampa, FL 33612, USA","institution_ids":["https://openalex.org/I2613432"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Aneeshea Cason","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Aneeshea Cason","raw_affiliation_strings":["Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida","Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida, Tampa, FL 33612, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida","institution_ids":["https://openalex.org/I2613432"]},{"raw_affiliation_string":"Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida, Tampa, FL 33612, USA","institution_ids":["https://openalex.org/I2613432"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108897904","display_name":"Juliana Gonzalez Alvarado","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Juliana Gonzalez Alvarado","raw_affiliation_strings":["Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida","Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida, Tampa, FL 33612, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida","institution_ids":["https://openalex.org/I2613432"]},{"raw_affiliation_string":"Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida, Tampa, FL 33612, USA","institution_ids":["https://openalex.org/I2613432"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5042865435","display_name":"Manya Bhandary","orcid":"https://orcid.org/0000-0002-6742-1550","observed_orcids":["https://orcid.org/0000-0002-6742-1550"]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Manya Bhandary","raw_affiliation_strings":["Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida","Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida, Tampa, FL 33612, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida","institution_ids":["https://openalex.org/I2613432"]},{"raw_affiliation_string":"Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida, Tampa, FL 33612, USA","institution_ids":["https://openalex.org/I2613432"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102445318","display_name":"Shreena Patel","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Shreena Patel","raw_affiliation_strings":["Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida","Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida, Tampa, FL 33612, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida","institution_ids":["https://openalex.org/I2613432"]},{"raw_affiliation_string":"Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida, Tampa, FL 33612, USA","institution_ids":["https://openalex.org/I2613432"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5006574635","display_name":"Julie Pratibha Singh","orcid":"https://orcid.org/0009-0004-4159-6995","observed_orcids":["https://orcid.org/0009-0004-4159-6995"]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Julie Singh","raw_affiliation_strings":["Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida","Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida, Tampa, FL 33612, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida","institution_ids":["https://openalex.org/I2613432"]},{"raw_affiliation_string":"Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida, Tampa, FL 33612, USA","institution_ids":["https://openalex.org/I2613432"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057362562","display_name":"Richard F. Heller","orcid":"https://orcid.org/0000-0003-1899-3859","observed_orcids":["https://orcid.org/0000-0003-1899-3859"]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Richard Heller","raw_affiliation_strings":["Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida","Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida, Tampa, FL 33612, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida","institution_ids":["https://openalex.org/I2613432"]},{"raw_affiliation_string":"Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida, Tampa, FL 33612, USA","institution_ids":["https://openalex.org/I2613432"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5048547302","display_name":"Lore\u00e9 C. Heller","orcid":"https://orcid.org/0000-0001-8084-0787","observed_orcids":["https://orcid.org/0000-0001-8084-0787"]},"institutions":[{"id":"https://openalex.org/I2613432","display_name":"University of South Florida","ror":"https://ror.org/032db5x82","country_code":"US","type":"education","lineage":["https://openalex.org/I2613432"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Loree C. Heller","raw_affiliation_strings":["Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida","Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida, Tampa, FL 33612, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida","institution_ids":["https://openalex.org/I2613432"]},{"raw_affiliation_string":"Department of Medical Engineering, Morsani College of Medicine and College of Engineering, University of South Florida, Tampa, FL 33612, USA","institution_ids":["https://openalex.org/I2613432"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5048547302"],"corresponding_institution_ids":["https://openalex.org/I2613432"],"apc_list":{"value":2500,"currency":"USD","value_usd":2500},"apc_paid":{"value":2500,"currency":"USD","value_usd":2500},"fwci":0.0,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":{"value":0.00791903,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"4","issue":"1","first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.7445999979972839,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.7445999979972839,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10441","display_name":"Muscle Physiology and Disorders","score":0.2094999998807907,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12400","display_name":"Neurogenetic and Muscular Disorders Research","score":0.011699999682605267,"subfield":{"id":"https://openalex.org/subfields/2716","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/plasmid-transfection","display_name":"plasmid transfection","score":0.9900000095367432},{"id":"https://openalex.org/keywords/c2c12-myotubes","display_name":"C2C12 myotubes","score":0.984000027179718},{"id":"https://openalex.org/keywords/skeletal-muscle-model","display_name":"skeletal muscle model","score":0.5699999928474426},{"id":"https://openalex.org/keywords/electroporation","display_name":"electroporation","score":0.9300000071525574},{"id":"https://openalex.org/keywords/transfection-efficiency","display_name":"transfection efficiency","score":0.9710000157356262},{"id":"https://openalex.org/keywords/cell-death","display_name":"cell death","score":0.9589999914169312},{"id":"https://openalex.org/keywords/proinflammatory-chemokines","display_name":"inflammatory chemokines","score":0.9829999804496765},{"id":"https://openalex.org/keywords/muscle-cell-culture","display_name":"muscle cell culture","score":0.5360000133514404},{"id":"https://openalex.org/keywords/reporter-plasmid","display_name":"reporter plasmid","score":0.8149999976158142}],"concepts":[{"id":"https://openalex.org/C60278653","wikidata":"https://www.wikidata.org/wiki/Q5008075","display_name":"C2C12","level":4,"score":0.902930498123169},{"id":"https://openalex.org/C3763915","wikidata":"https://www.wikidata.org/wiki/Q3331437","display_name":"Myogenesis","level":3,"score":0.8854870796203613},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.7660881280899048},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.7303409576416016},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.47044745087623596},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3834117650985718},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.3670947849750519},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.33275893330574036},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.32349759340286255},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.2825278043746948},{"id":"https://openalex.org/C207200792","wikidata":"https://www.wikidata.org/wiki/Q428914","display_name":"Myocyte","level":2,"score":0.2728824019432068},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.19187778234481812}],"mesh":[],"locations_count":3,"locations":[{"id":"doi:10.1089/regen.2023.0026","doi":"https://doi.org/10.1089/regen.2023.0026","is_oa":true,"landing_page_url":"http://dx.doi.org/10.1089/regen.2023.0026","pdf_url":null,"source":{"id":"https://openalex.org/S4210214794","display_name":"Re GEN Open","issn_l":"2766-2705","issn":["2766-2705"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Re:GEN Open","raw_type":"journal-article"},{"id":"pmh:oai:doaj.org/article:2abf949102394603b2ab57d98fcce18f","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/2abf949102394603b2ab57d98fcce18f","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Re:GEN Open, Vol 4, Iss 1, Pp 1-8 (2024)","raw_type":"article"},{"id":"pmh:oai:doaj.org/article:e2e1260046754ded9ad3ab39116d0be1","doi":null,"is_oa":false,"landing_page_url":"https://doaj.org/article/e2e1260046754ded9ad3ab39116d0be1","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Re:GEN Open, Vol 4, Iss 1, Pp 1-8 (2024)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1089/regen.2023.0026","doi":"https://doi.org/10.1089/regen.2023.0026","is_oa":true,"landing_page_url":"http://dx.doi.org/10.1089/regen.2023.0026","pdf_url":null,"source":{"id":"https://openalex.org/S4210214794","display_name":"Re GEN Open","issn_l":"2766-2705","issn":["2766-2705"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320443","host_organization_name":"Mary Ann Liebert, Inc.","host_organization_lineage":["https://openalex.org/P4310320443","https://openalex.org/P4310320017"],"host_organization_lineage_names":["Mary Ann Liebert, Inc.","SAGE Publishing"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Re:GEN Open","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.8199999928474426,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":37,"referenced_works":["https://openalex.org/W1655460658","https://openalex.org/W1904722209","https://openalex.org/W1968284072","https://openalex.org/W1970244619","https://openalex.org/W1982770605","https://openalex.org/W1983487014","https://openalex.org/W1986063846","https://openalex.org/W1991227528","https://openalex.org/W1997069349","https://openalex.org/W1999674600","https://openalex.org/W2026718882","https://openalex.org/W2029635184","https://openalex.org/W2031892511","https://openalex.org/W2035896578","https://openalex.org/W2049346145","https://openalex.org/W2055866192","https://openalex.org/W2057388489","https://openalex.org/W2061142961","https://openalex.org/W2063785259","https://openalex.org/W2074939226","https://openalex.org/W2079187308","https://openalex.org/W2096173059","https://openalex.org/W2122322408","https://openalex.org/W2131738138","https://openalex.org/W2163517119","https://openalex.org/W2318823488","https://openalex.org/W2325056821","https://openalex.org/W2580020392","https://openalex.org/W2580035316","https://openalex.org/W2791955556","https://openalex.org/W2793221955","https://openalex.org/W2948325732","https://openalex.org/W2966261467","https://openalex.org/W2973145297","https://openalex.org/W3022838598","https://openalex.org/W3112376646","https://openalex.org/W4303962147"],"related_works":["https://openalex.org/W4306928302","https://openalex.org/W4286855968","https://openalex.org/W4255478201","https://openalex.org/W2101160857","https://openalex.org/W2086721145","https://openalex.org/W1991227528","https://openalex.org/W1983415286","https://openalex.org/W2809966715","https://openalex.org/W2009870936","https://openalex.org/W7097698397"],"abstract_inverted_index":{"Background:":[0],"Models":[1],"of":[2,26,61,64,102,151,192],"muscle":[3,20,50],"cell":[4,28,84,130,147],"culture":[5,29],"allow":[6],"simple":[7],"manipulation":[8],"to":[9,18,57,188],"optimize":[10],"gene":[11,36],"transfer":[12,37],"experiments,":[13],"offering":[14],"a":[15,32,48,124,140,179],"significant":[16],"opportunity":[17],"model":[19,194],"effects":[21],"by":[22,67,92,160],"allowing":[23],"precise":[24],"control":[25],"the":[27,59,100,190],"environment":[30],"in":[31,40,195],"cost-effective":[33],"manner.":[34],"Often,":[35],"is":[38],"performed":[39],"myoblasts,":[41],"which":[42,157,176],"are":[43,90,186],"then":[44],"differentiated":[45,65,168],"into":[46],"myotubes,":[47],"skeletal":[49,161],"model.":[51,182],"In":[52,108],"this":[53,109,193],"study,":[54,110],"we":[55,111],"aimed":[56],"compare":[58],"efficiency":[60,82,143],"direct":[62],"transfection":[63,142],"myotubes":[66,75,115,132,169],"chemical":[68,125],"and":[69,83,94],"physical":[70],"plasmid":[71],"methods.":[72,122],"Methods:":[73],"Differentiated":[74],"were":[76,86,133],"transfected":[77,119,173],"with":[78,145],"reporter":[79],"plasmids.":[80],"Transfection":[81],"death":[85],"quantified.":[87,106],"Proinflammatory":[88],"proteins":[89,154],"produced":[91,139],"cells":[93],"tissues":[95],"after":[96],"DNA":[97],"transfection;":[98],"therefore,":[99],"secretion":[101,159],"proinflammatory":[103,152],"chemokines":[104],"was":[105,155],"Results:":[107],"demonstrate":[112],"that":[113,167],"C2C12":[114],"can":[116,170],"be":[117,171],"effectively":[118],"using":[120],"multiple":[121],"While":[123],"method":[126],"did":[127],"not":[128,134],"cause":[129],"death,":[131],"efficiently":[135],"transfected.":[136],"Electroporation":[137],"delivery":[138],"higher":[141,146],"coupled":[144],"death.":[148],"A":[149],"subset":[150],"chemokine":[153],"secreted,":[156],"replicated":[158],"muscle.":[162],"Discussion:":[163],"These":[164],"results":[165],"suggest":[166],"directly":[172],"invitro":[174],",":[175],"may":[177],"represent":[178],"useful":[180],"experimental":[181],"However,":[183],"more":[184],"studies":[185],"needed":[187],"assess":[189],"value":[191],"predicting":[196],"invivo":[197],"responses.":[198]},"counts_by_year":[],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2024-02-01T00:00:00"}