{"id":"https://openalex.org/W4390914192","doi":"https://doi.org/10.1007/s10811-023-03179-6","title":"Semi-continuous cultivation for enhanced protein production using indigenous green microalgae and synthetic municipal wastewater","display_name":"Semi-continuous cultivation for enhanced protein production using indigenous green microalgae and synthetic municipal wastewater","publication_year":2024,"publication_date":"2024-01-16","ids":{"openalex":"https://openalex.org/W4390914192","doi":"https://doi.org/10.1007/s10811-023-03179-6"},"language":"en","primary_location":{"id":"doi:10.1007/s10811-023-03179-6","doi":"https://doi.org/10.1007/s10811-023-03179-6","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s10811-023-03179-6","pdf_url":"https://link.springer.com/content/pdf/10.1007/s10811-023-03179-6.pdf","source":{"id":"https://openalex.org/S4121844","display_name":"Journal of Applied Phycology","issn_l":"0921-8971","issn":["0921-8971","1573-5176"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Applied Phycology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://link.springer.com/content/pdf/10.1007/s10811-023-03179-6.pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5120916346","display_name":"Umetani Ikumi","orcid":"https://orcid.org/0000-0003-3197-9277","observed_orcids":["https://orcid.org/0000-0003-3197-9277"]},"institutions":[{"id":"https://openalex.org/I4210143403","display_name":"Norwegian Institute of Bioeconomy Research","ror":"https://ror.org/04aah1z61","country_code":"NO","type":"funder","lineage":["https://openalex.org/I4210143403"]}],"countries":["NO"],"is_corresponding":true,"raw_author_name":"Ikumi Umetani","raw_affiliation_strings":["Norwegian Institute of Bioeconomy Research, \u00c5s, Norway"],"raw_orcid":"https://orcid.org/0000-0003-3197-9277","affiliations":[{"raw_affiliation_string":"Norwegian Institute of Bioeconomy Research, \u00c5s, Norway","institution_ids":["https://openalex.org/I4210143403"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5019325494","display_name":"Micha\u0142 Spos\u00f3b","orcid":"https://orcid.org/0000-0001-6656-8203","observed_orcids":["https://orcid.org/0000-0001-6656-8203"]},"institutions":[{"id":"https://openalex.org/I4210143403","display_name":"Norwegian Institute of Bioeconomy Research","ror":"https://ror.org/04aah1z61","country_code":"NO","type":"funder","lineage":["https://openalex.org/I4210143403"]}],"countries":["NO"],"is_corresponding":false,"raw_author_name":"Micha\u0142 Spos\u00f3b","raw_affiliation_strings":["Norwegian Institute of Bioeconomy Research, \u00c5s, Norway"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Norwegian Institute of Bioeconomy Research, \u00c5s, Norway","institution_ids":["https://openalex.org/I4210143403"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5037929361","display_name":"Olga Tiron","orcid":"https://orcid.org/0000-0001-9810-4999","observed_orcids":["https://orcid.org/0000-0001-9810-4999"]},"institutions":[{"id":"https://openalex.org/I4210134874","display_name":"National Institute for Research and Development for Industrial Ecology","ror":"https://ror.org/02scqqt19","country_code":"RO","type":"education","lineage":["https://openalex.org/I4210134874"]}],"countries":["RO"],"is_corresponding":false,"raw_author_name":"Olga Tiron","raw_affiliation_strings":["National Research and Development Institute for Industrial Ecology, Bucharest, Romania"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"National Research and Development Institute for Industrial Ecology, Bucharest, Romania","institution_ids":["https://openalex.org/I4210134874"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5120916346"],"corresponding_institution_ids":["https://openalex.org/I4210143403"],"apc_list":{"value":3690,"currency":"USD","value_usd":3690},"apc_paid":{"value":3690,"currency":"USD","value_usd":3690},"fwci":0.8831,"has_fulltext":true,"cited_by_count":13,"citation_normalized_percentile":{"value":0.65900407,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":93,"max":98},"biblio":{"volume":"36","issue":"3","first_page":"1105","last_page":"1116"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10476","display_name":"Algal biology and biofuel production","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2105","display_name":"Renewable Energy, Sustainability and the Environment"},"field":{"id":"https://openalex.org/fields/21","display_name":"Energy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10476","display_name":"Algal biology and biofuel production","score":0.9994999766349792,"subfield":{"id":"https://openalex.org/subfields/2105","display_name":"Renewable Energy, Sustainability and the Environment"},"field":{"id":"https://openalex.org/fields/21","display_name":"Energy"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10236","display_name":"Aquatic Ecosystems and Phytoplankton Dynamics","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2304","display_name":"Environmental Chemistry"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10450","display_name":"Aquaculture Nutrition and Growth","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1104","display_name":"Aquatic Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/semi-continuous-cultivation","display_name":"semi-continuous cultivation","score":0.9369999766349792},{"id":"https://openalex.org/keywords/klebsormidium-flaccidum","display_name":"Klebsormidium flaccidum","score":1.0},{"id":"https://openalex.org/keywords/municipal-wastewater-treatment","display_name":"municipal wastewater treatment","score":0.7390000224113464},{"id":"https://openalex.org/keywords/nutrient-removal","display_name":"nutrient removal","score":0.7480000257492065},{"id":"https://openalex.org/keywords/nitrogen-removal","display_name":"nitrogen removal","score":0.9039999842643738},{"id":"https://openalex.org/keywords/phosphorus-removal","display_name":"phosphorus removal","score":0.953000009059906},{"id":"https://openalex.org/keywords/chlorophyta","display_name":"Chlorophyta","score":0.5960000157356262},{"id":"https://openalex.org/keywords/biomass-productivity","display_name":"biomass productivity","score":0.7310000061988831},{"id":"https://openalex.org/keywords/amino-acid-composition","display_name":"amino acid composition","score":0.6579999923706055},{"id":"https://openalex.org/keywords/glutamate","display_name":"glutamate","score":0.8579999804496765},{"id":"https://openalex.org/keywords/nitrogen-and-phosphorus-deficiency","display_name":"nitrogen and phosphorus deficiency","score":0.5450000166893005}],"concepts":[{"id":"https://openalex.org/C94061648","wikidata":"https://www.wikidata.org/wiki/Q336191","display_name":"Wastewater","level":2,"score":0.7564905881881714},{"id":"https://openalex.org/C115540264","wikidata":"https://www.wikidata.org/wiki/Q2945560","display_name":"Biomass (ecology)","level":2,"score":0.6755294799804688},{"id":"https://openalex.org/C142796444","wikidata":"https://www.wikidata.org/wiki/Q181394","display_name":"Nutrient","level":2,"score":0.6346558332443237},{"id":"https://openalex.org/C42566525","wikidata":"https://www.wikidata.org/wiki/Q652439","display_name":"Single-cell protein","level":3,"score":0.5428526401519775},{"id":"https://openalex.org/C510538283","wikidata":"https://www.wikidata.org/wiki/Q674","display_name":"Phosphorus","level":2,"score":0.48277416825294495},{"id":"https://openalex.org/C528095902","wikidata":"https://www.wikidata.org/wiki/Q2283886","display_name":"Pulp and paper industry","level":1,"score":0.47549259662628174},{"id":"https://openalex.org/C57442070","wikidata":"https://www.wikidata.org/wiki/Q221275","display_name":"Sewage treatment","level":2,"score":0.4439815580844879},{"id":"https://openalex.org/C204983608","wikidata":"https://www.wikidata.org/wiki/Q2111958","display_name":"Productivity","level":2,"score":0.4248961806297302},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.39737945795059204},{"id":"https://openalex.org/C31903555","wikidata":"https://www.wikidata.org/wiki/Q1637030","display_name":"Food science","level":1,"score":0.3971063494682312},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.3595923185348511},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3441844582557678},{"id":"https://openalex.org/C6557445","wikidata":"https://www.wikidata.org/wiki/Q173113","display_name":"Agronomy","level":1,"score":0.2708236277103424},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.20874953269958496},{"id":"https://openalex.org/C39432304","wikidata":"https://www.wikidata.org/wiki/Q188847","display_name":"Environmental science","level":0,"score":0.20456495881080627},{"id":"https://openalex.org/C87717796","wikidata":"https://www.wikidata.org/wiki/Q146326","display_name":"Environmental engineering","level":1,"score":0.204012930393219},{"id":"https://openalex.org/C18903297","wikidata":"https://www.wikidata.org/wiki/Q7150","display_name":"Ecology","level":1,"score":0.10420697927474976},{"id":"https://openalex.org/C139719470","wikidata":"https://www.wikidata.org/wiki/Q39680","display_name":"Macroeconomics","level":1,"score":0.0},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.0},{"id":"https://openalex.org/C162324750","wikidata":"https://www.wikidata.org/wiki/Q8134","display_name":"Economics","level":0,"score":0.0}],"mesh":[],"locations_count":1,"locations":[{"id":"doi:10.1007/s10811-023-03179-6","doi":"https://doi.org/10.1007/s10811-023-03179-6","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s10811-023-03179-6","pdf_url":"https://link.springer.com/content/pdf/10.1007/s10811-023-03179-6.pdf","source":{"id":"https://openalex.org/S4121844","display_name":"Journal of Applied Phycology","issn_l":"0921-8971","issn":["0921-8971","1573-5176"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Applied Phycology","raw_type":"journal-article"}],"best_oa_location":{"id":"doi:10.1007/s10811-023-03179-6","doi":"https://doi.org/10.1007/s10811-023-03179-6","is_oa":true,"landing_page_url":"https://doi.org/10.1007/s10811-023-03179-6","pdf_url":"https://link.springer.com/content/pdf/10.1007/s10811-023-03179-6.pdf","source":{"id":"https://openalex.org/S4121844","display_name":"Journal of Applied Phycology","issn_l":"0921-8971","issn":["0921-8971","1573-5176"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","jufo-1","ki-jl-1","norway-1","poland-70","russia-white-list-1"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Applied Phycology","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Clean water and sanitation","id":"https://metadata.un.org/sdg/6","score":1.0},{"display_name":"Responsible consumption and production","id":"https://metadata.un.org/sdg/12","score":1.0}],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320336013","display_name":"Norsk institutt for Bio\u00f8konomi","ror":null}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4390914192.pdf","grobid_xml":"https://content.openalex.org/works/W4390914192.grobid-xml"},"referenced_works_count":37,"referenced_works":["https://openalex.org/W108293693","https://openalex.org/W1775749144","https://openalex.org/W1844053279","https://openalex.org/W1940503652","https://openalex.org/W1969756922","https://openalex.org/W1980066432","https://openalex.org/W2006317131","https://openalex.org/W2036414628","https://openalex.org/W2048265950","https://openalex.org/W2147484531","https://openalex.org/W2162336404","https://openalex.org/W2171520441","https://openalex.org/W2434397641","https://openalex.org/W2465916950","https://openalex.org/W2503603657","https://openalex.org/W2732565512","https://openalex.org/W2763176525","https://openalex.org/W2905027421","https://openalex.org/W2986546288","https://openalex.org/W2999995902","https://openalex.org/W3002518526","https://openalex.org/W3094915189","https://openalex.org/W3107753211","https://openalex.org/W3152653058","https://openalex.org/W3156041323","https://openalex.org/W3167487540","https://openalex.org/W3188189453","https://openalex.org/W4200321590","https://openalex.org/W4214526623","https://openalex.org/W4220883291","https://openalex.org/W4221018586","https://openalex.org/W4223450741","https://openalex.org/W4224287240","https://openalex.org/W4224994256","https://openalex.org/W4280495362","https://openalex.org/W4283652189","https://openalex.org/W4377115873"],"related_works":["https://openalex.org/W2015785904","https://openalex.org/W3037847011","https://openalex.org/W3157396736","https://openalex.org/W4385520159","https://openalex.org/W2793887079","https://openalex.org/W2157614933","https://openalex.org/W4377115873","https://openalex.org/W3087914285","https://openalex.org/W2807644587","https://openalex.org/W2901803430"],"abstract_inverted_index":{"Abstract":[0],"Cultivation":[1],"of":[2,34,65,128,132,170,194,229],"microalgae":[3],"has":[4],"gained":[5],"significant":[6],"interest":[7],"as":[8],"an":[9,226],"alternative":[10],"protein":[11,32,126,149,222],"source,":[12],"potentially":[13],"becoming":[14],"a":[15,26,58,70,98,139,214],"target":[16],"commodity":[17],"recovered":[18],"from":[19,48,231],"microalgae-based":[20],"wastewater":[21,49],"treatment.":[22],"This":[23],"study":[24],"examined":[25],"semi-continuous":[27],"cultivation":[28,67,89,210],"strategy":[29],"to":[30,84],"optimize":[31],"accumulation":[33],"the":[35,63,85,124,163,167,208,232],"indigenous":[36],"freshwater":[37],"chlorophytes,":[38],"Lobochlamys":[39,102],"segnis":[40,103,155],"and":[41,44,78,104,117,130,145,159,172,220],"Klebsormidium":[42],"flaccidum,":[43],"simultaneously":[45],"remove":[46],"nutrients":[47],"efficiently.":[50],"A":[51],"strain-specific":[52],"regime":[53,211],"was":[54,115,147,151,197],"made":[55],"based":[56],"on":[57],"fixed":[59],"biomass":[60,113,216],"concentration":[61],"at":[62],"start":[64],"24-h":[66],"cycle,":[68],"i.e.,":[69],"constant":[71,215],"initial":[72],"cell":[73,134],"density,":[74],"which":[75,123],"regulated":[76],"harvesting":[77],"fresh":[79],"medium":[80,141,164],"supply":[81],"volume":[82],"according":[83],"dilution":[86],"rate.":[87],"Six":[88],"cycles":[90],"were":[91],"conducted":[92],"in":[93,109,122,143],"lab-scale":[94],"1L":[95],"reactors":[96],"with":[97,218,225],"synthetic":[99],"municipal":[100],"wastewater.":[101,233],"K.":[105,178],"flaccidum":[106,179],"grew":[107],"exponentially":[108],"all":[110,157],"cycles.":[111],"The":[112,190],"productivity":[114],"573":[116],"580":[118],"mg":[119,174,182],"L\u20131":[120,175,183],"day\u20131,":[121],"total":[125],"consisted":[127],"62":[129],"45%":[131],"dry":[133],"weight":[135],"(dw),":[136],"respectively.":[137],"When":[138],"culture":[140],"deficient":[142],"nitrogen":[144],"phosphorus":[146],"used,":[148],"level":[150],"significantly":[152],"reduced.":[153],"L.":[154],"consumed":[156],"NH4+":[158,185],"PO43\u2013":[160,188],"supplied":[161],"by":[162,199],"replacement,":[165],"giving":[166],"removal":[168,228],"rate":[169],"9.2":[171],"5.2":[173],"day\u20131.":[176],"Whereas":[177],"removed":[180],"13.8":[181],"day\u20131":[184],"without":[186],"completing":[187],"removal.":[189],"amino":[191],"acid":[192],"profile":[193],"both":[195],"strains":[196],"characterized":[198],"glutamic":[200],"acids":[201],"content":[202],"(4\u20135%":[203],"dw).":[204],"We":[205],"concluded":[206],"that":[207],"designed":[209],"would":[212],"support":[213],"production":[217],"stable":[219],"high":[221],"content,":[223],"along":[224],"efficient":[227],"nutrient":[230]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":6},{"year":2024,"cited_by_count":2}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2024-01-17T00:00:00"}