{"id":"https://openalex.org/W4390880856","doi":"https://doi.org/10.3390/microorganisms12010169","title":"Parenteral Vaccination with a Live Brucella melitensis Mutant Protects against Wild-Type B. melitensis 16M Challenge","display_name":"Parenteral Vaccination with a Live Brucella melitensis Mutant Protects against Wild-Type B. melitensis 16M Challenge","publication_year":2024,"publication_date":"2024-01-15","ids":{"openalex":"https://openalex.org/W4390880856","doi":"https://doi.org/10.3390/microorganisms12010169","pmid":"https://pubmed.ncbi.nlm.nih.gov/38257995","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/10820470"},"language":"en","primary_location":{"id":"doi:10.3390/microorganisms12010169","doi":"https://doi.org/10.3390/microorganisms12010169","is_oa":true,"landing_page_url":"https://doi.org/10.3390/microorganisms12010169","pdf_url":"https://www.mdpi.com/2076-2607/12/1/169/pdf?version=1705293389","source":{"id":"https://openalex.org/S4210222090","display_name":"Microorganisms","issn_l":"2076-2607","issn":["2076-2607"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microorganisms","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://www.mdpi.com/2076-2607/12/1/169/pdf?version=1705293389","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5026875926","display_name":"Xinghong Yang","orcid":"https://orcid.org/0000-0001-5629-3357","observed_orcids":["https://orcid.org/0000-0001-5629-3357"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Xinghong Yang","raw_affiliation_strings":["Department of Infectious Diseases & Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Infectious Diseases & Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054119420","display_name":"Zakia I. Goodwin","orcid":"https://orcid.org/0000-0003-0217-2343","observed_orcids":["https://orcid.org/0000-0003-0217-2343"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Zakia I. Goodwin","raw_affiliation_strings":["Department of Infectious Diseases & Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Infectious Diseases & Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012252242","display_name":"Ella Bhagyaraj","orcid":"https://orcid.org/0000-0002-5887-9228","observed_orcids":["https://orcid.org/0000-0002-5887-9228"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Ella Bhagyaraj","raw_affiliation_strings":["Department of Infectious Diseases & Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Infectious Diseases & Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5014310365","display_name":"Carol A. Hoffman","orcid":"https://orcid.org/0000-0003-3355-7174","observed_orcids":["https://orcid.org/0000-0003-3355-7174"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Carol Hoffman","raw_affiliation_strings":["Department of Infectious Diseases & Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Infectious Diseases & Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5103066905","display_name":"David W. Pascual","orcid":"https://orcid.org/0000-0002-6572-6916","observed_orcids":["https://orcid.org/0000-0002-6572-6916"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"David W. Pascual","raw_affiliation_strings":["Department of Infectious Diseases & Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Infectious Diseases & Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA","institution_ids":["https://openalex.org/I33213144"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5103066905"],"corresponding_institution_ids":["https://openalex.org/I33213144"],"apc_list":{"value":2700,"currency":"CHF","value_usd":3069},"apc_paid":{"value":2700,"currency":"CHF","value_usd":3069},"fwci":1.4483,"has_fulltext":true,"cited_by_count":3,"citation_normalized_percentile":{"value":0.75583044,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":97},"biblio":{"volume":"12","issue":"1","first_page":"169","last_page":"169"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11899","display_name":"Brucella: diagnosis, epidemiology, treatment","score":1.0,"subfield":{"id":"https://openalex.org/subfields/3404","display_name":"Small Animals"},"field":{"id":"https://openalex.org/fields/34","display_name":"Veterinary"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11899","display_name":"Brucella: diagnosis, epidemiology, treatment","score":1.0,"subfield":{"id":"https://openalex.org/subfields/3404","display_name":"Small Animals"},"field":{"id":"https://openalex.org/fields/34","display_name":"Veterinary"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10873","display_name":"Bacterial Infections and Vaccines","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11688","display_name":"Microbial infections and disease research","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/brucella-melitensis","display_name":"Brucella melitensis","score":0.9779999852180481},{"id":"https://openalex.org/keywords/live-attenuated-vaccine","display_name":"live attenuated vaccine","score":0.8659999966621399},{"id":"https://openalex.org/keywords/parenteral-immunization","display_name":"parenteral immunization","score":0.8299999833106995},{"id":"https://openalex.org/keywords/brucella-abortus-s19","display_name":"Brucella abortus S19","score":0.925000011920929},{"id":"https://openalex.org/keywords/brucellosis-vaccine","display_name":"brucellosis vaccine","score":0.9200000166893005},{"id":"https://openalex.org/keywords/systemic-immunity","display_name":"systemic immunity","score":0.5849999785423279},{"id":"https://openalex.org/keywords/cd8-t-cell-immunity","display_name":"CD8+ T cell immunity","score":0.7289999723434448},{"id":"https://openalex.org/keywords/cd4-t-cell-immunity","display_name":"CD4+ T cell immunity","score":0.9139999747276306},{"id":"https://openalex.org/keywords/effector-memory-t-cells","display_name":"effector memory T cells","score":0.8949999809265137},{"id":"https://openalex.org/keywords/interferon-gamma","display_name":"interferon-gamma","score":0.7170000076293945},{"id":"https://openalex.org/keywords/macrophage-replication","display_name":"macrophage replication","score":0.6909999847412109},{"id":"https://openalex.org/keywords/livestock-vaccination","display_name":"livestock vaccination","score":0.4480000138282776}],"concepts":[{"id":"https://openalex.org/C2777692091","wikidata":"https://www.wikidata.org/wiki/Q3512031","display_name":"Brucella melitensis","level":4,"score":0.9479057192802429},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6388314962387085},{"id":"https://openalex.org/C22070199","wikidata":"https://www.wikidata.org/wiki/Q192995","display_name":"Vaccination","level":2,"score":0.5130680799484253},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.5058355331420898},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.472187876701355},{"id":"https://openalex.org/C207583985","wikidata":"https://www.wikidata.org/wiki/Q128723","display_name":"Wild type","level":4,"score":0.46447598934173584},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4361557364463806},{"id":"https://openalex.org/C2780767784","wikidata":"https://www.wikidata.org/wiki/Q313358","display_name":"Brucella","level":3,"score":0.40762627124786377},{"id":"https://openalex.org/C2781429187","wikidata":"https://www.wikidata.org/wiki/Q156050","display_name":"Brucellosis","level":2,"score":0.1989448070526123},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.1605949103832245},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08178222179412842}],"mesh":[],"locations_count":4,"locations":[{"id":"doi:10.3390/microorganisms12010169","doi":"https://doi.org/10.3390/microorganisms12010169","is_oa":true,"landing_page_url":"https://doi.org/10.3390/microorganisms12010169","pdf_url":"https://www.mdpi.com/2076-2607/12/1/169/pdf?version=1705293389","source":{"id":"https://openalex.org/S4210222090","display_name":"Microorganisms","issn_l":"2076-2607","issn":["2076-2607"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microorganisms","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:10820470","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/10820470","pdf_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC10820470/pdf/microorganisms-12-00169.pdf","source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microorganisms","raw_type":"Text"},{"id":"pmid:38257995","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/38257995","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microorganisms","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:0221299f62f04ea1a3868c239421af3f","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/0221299f62f04ea1a3868c239421af3f","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Microorganisms, Vol 12, Iss 1, p 169 (2024)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.3390/microorganisms12010169","doi":"https://doi.org/10.3390/microorganisms12010169","is_oa":true,"landing_page_url":"https://doi.org/10.3390/microorganisms12010169","pdf_url":"https://www.mdpi.com/2076-2607/12/1/169/pdf?version=1705293389","source":{"id":"https://openalex.org/S4210222090","display_name":"Microorganisms","issn_l":"2076-2607","issn":["2076-2607"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","norway-1","poland-40","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310310987","host_organization_name":"Multidisciplinary Digital Publishing Institute","host_organization_lineage":["https://openalex.org/P4310310987"],"host_organization_lineage_names":["Multidisciplinary Digital Publishing Institute"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microorganisms","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being","score":0.9391000270843506}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3082329728","display_name":"Mucosal Vaccines for Brucellosis","funder_award_id":"R01AI123244","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G8309725131","display_name":"Naso-oropharyngeal Brucella Infections and Mucosal Immune Protection","funder_award_id":"R01AI125516","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W4390880856.pdf","grobid_xml":"https://content.openalex.org/works/W4390880856.grobid-xml"},"referenced_works_count":49,"referenced_works":["https://openalex.org/W335465506","https://openalex.org/W1723305916","https://openalex.org/W1771527499","https://openalex.org/W1841679394","https://openalex.org/W1856892166","https://openalex.org/W1970338615","https://openalex.org/W1979310596","https://openalex.org/W1984027820","https://openalex.org/W1988026832","https://openalex.org/W1988645306","https://openalex.org/W1995222343","https://openalex.org/W1997800481","https://openalex.org/W2002024993","https://openalex.org/W2009273089","https://openalex.org/W2010635077","https://openalex.org/W2013034108","https://openalex.org/W2019260263","https://openalex.org/W2032013011","https://openalex.org/W2109293528","https://openalex.org/W2125042173","https://openalex.org/W2133176880","https://openalex.org/W2135715402","https://openalex.org/W2135847852","https://openalex.org/W2142622505","https://openalex.org/W2146612401","https://openalex.org/W2153484185","https://openalex.org/W2167016442","https://openalex.org/W2170936249","https://openalex.org/W2188979598","https://openalex.org/W2237942997","https://openalex.org/W2327762713","https://openalex.org/W2520352594","https://openalex.org/W2602299919","https://openalex.org/W2749212869","https://openalex.org/W2808768879","https://openalex.org/W2905337655","https://openalex.org/W2961226555","https://openalex.org/W2999621125","https://openalex.org/W3023843036","https://openalex.org/W3082788765","https://openalex.org/W3127121858","https://openalex.org/W3154941305","https://openalex.org/W4211033510","https://openalex.org/W4294225486","https://openalex.org/W4300963144","https://openalex.org/W4312187566","https://openalex.org/W4313333789","https://openalex.org/W4320913880","https://openalex.org/W4363646103"],"related_works":["https://openalex.org/W4300963144","https://openalex.org/W2520352594","https://openalex.org/W3089022109","https://openalex.org/W2107896108","https://openalex.org/W2756143854","https://openalex.org/W2130785720","https://openalex.org/W2115617534","https://openalex.org/W2888148578","https://openalex.org/W2042575699","https://openalex.org/W2035694088"],"abstract_inverted_index":{"Susceptibility":[0],"to":[1,15,53,84,130,144,196],"brucellosis":[2,19],"remains":[3],"prevalent,":[4],"even":[5],"in":[6],"herds":[7],"vaccinated":[8,115],"with":[9,78,93,121],"conventional":[10],"vaccines.":[11],"Efforts":[12],"are":[13,30,193],"underway":[14],"develop":[16],"an":[17],"improved":[18],"vaccine,":[20,25],"and":[21,44,47,71,81,153,199,207],"possibly":[22],"a":[23,205],"universal":[24],"given":[26],"that":[27,188],"Brucella":[28],"species":[29],"highly":[31,208],"homologous.":[32],"To":[33,62,104],"this":[34],"end,":[35],"two":[36],"B.":[37,59,125],"melitensis":[38,60],"mutants":[39,192],"were":[40,48,76,114],"developed,":[41],"znBM-lacZ":[42],"(znBMZ)":[43],"znBM-mCherry":[45],"(znBM-mC),":[46],"tested":[49],"for":[50,106,146,215],"their":[51,67],"ability":[52],"confer":[54],"systemic":[55,109],"immunity":[56,203],"against":[57,108],"virulent":[58],"challenge.":[61],"assess":[63],"the":[64,82,99,118,131,189],"extent":[65],"of":[66,96,112],"attenuation,":[68],"bone-marrow-derived":[69],"macrophages":[70],"human":[72],"TF-1":[73],"myeloid":[74],"cells":[75,88,177],"infected":[77,92],"both":[79,151,170],"mutants,":[80],"inability":[83],"replicate":[85],"within":[86,101],"these":[87,185],"was":[89],"noted.":[90],"Mice":[91],"varying":[94],"doses":[95],"znBM-mC":[97],"cleared":[98],"brucellae":[100,139],"6\u201310":[102],"weeks.":[103],"test":[105],"efficacy":[107],"disease,":[110],"groups":[111],"mice":[113],"once":[116],"by":[117,141],"intraperitoneal":[119],"route":[120],"either":[122],"znBMZ":[123,134,152,163,180],"or":[124],"abortus":[126],"S19":[127,154],"vaccine.":[128],"Relative":[129],"PBS-dosed":[132],"mice,":[133],"vaccination":[135],"greatly":[136],"reduced":[137],"splenic":[138],"colonization":[140],"~25,000-fold":[142],"compared":[143],"700-fold":[145],"S19-vaccinated":[147],"mice.":[148],"Not":[149],"surprisingly,":[150],"strains":[155,171],"induced":[156,164,172,181],"IFN-\u03b3+":[157,165],"CD4+":[158,173,198],"T":[159,167,176,201],"cells,":[160],"yet":[161],"only":[162,179],"CD8+":[166,182,200],"cells.":[168],"While":[169],"effector":[174],"memory":[175],"(Tems),":[178],"Tems.":[183],"Thus,":[184],"results":[186],"show":[187],"described":[190],"znBM":[191],"safe,":[194],"able":[195],"elicit":[197],"cell":[202],"without":[204],"boost,":[206],"effective,":[209],"rendering":[210],"them":[211],"promising":[212],"vaccine":[213],"candidates":[214],"livestock.":[216]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2024-01-16T00:00:00"}